Difference between revisions of "Team:Chalmers-Gothenburg/BioBricks"

 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
{{Template_All_Teams}}
+
{{Chalmers-Gothenburg}}
  
<!-- Declare that you are going to use html code instead of wiki code -->
+
<h2>BioBricks</h2>
<html>
+
  
<!-- Start of CSS-->
+
<h3>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h3>
<style type="text/css">
+
<p>Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from <i>STE2</i> (<i>Saccharomyces cerevisiae</i>) and Pheromone P-factor receptor <i>MAM2</i> (<i>Schizosaccharomyces pombe</i>) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from <i>S.pombe</i> through the Pheromone pathway in <i>S.cerevisiae</i>.</p>
  
/* PAGE LAYOUT */
+
<h3>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h3>
 
+
/* Change Background color*/
+
body {
+
background-color: #fff;
+
}
+
 
+
/* Creates a container that will wrap all of the content inside your wiki pages. */
+
#mainContainer { 
+
width: 978px;
+
overflow:hidden;
+
float:left;
+
margin-left:20px;
+
margin-bottom: 10px;
+
background-color: #fff;
+
border-bottom: 14px solid #565656;
+
border-right: 2px solid #565656;
+
border-left: 2px solid #565656;
+
border-top: 2px solid #565656;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
}
+
 
+
/* Creates the container for the menu */
+
#menuContainer  {
+
float:left;
+
width: 134px;
+
padding: 20px 0px;
+
border-top: 14px solid #565656;
+
background-color: #E8E8E9;
+
}
+
 
+
/* Creates the container for the content */
+
#contentContainer {
+
padding-top:20px;
+
padding-right:10px;
+
margin-bottom: 20px;
+
width: 814px;
+
padding-left: 20px;
+
float: left;
+
background-color: #fff;
+
border-top: 14px solid #565656;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
}
+
 
+
/*Set up height place holder for the banner*/
+
#bannerContainer {
+
height:200px;
+
margin:auto;
+
text-align:center;
+
color: #24B694;
+
}
+
 
+
/*VISUAL STYLE (TABLES, FONTS, LINKS, IMAGES, UL) */
+
 
+
/*Change the styling of text for everything inside main container*/
+
#mainContainer p {
+
font-size: 13px;
+
color: #000000;
+
}
+
+
/*This changes the color and font family */
+
#contentContainer h1, h2, h3, h4, h5, h6 {
+
color: #565656;
+
border-bottom: none;
+
font-weight: bold;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
margin-top:10px;
+
}
+
 
+
/*Style of the links - links are different inside the menu */
+
#contentContainer a {
+
font-weight: bold;
+
color: #23b593;
+
}
+
 
+
  /* Styling links on hover- links are different inside the menu */
+
#contentContainer a:hover {
+
color: #59bf92;
+
}
+
 
+
/*Change the styling of tables */
+
        #contentContainer table {
+
    border: 1px solid #565656;
+
    border-collapse: collapse;
+
    width: 90%
+
    margin: auto;
+
    margin-bottom: 15px;
+
    margin-top: 15px;
+
    margin-right: 10px;
+
    margin-left: 10px;
+
    }
+
 
+
/*Change the styling of table cells*/
+
    #contentContainer  td {
+
    padding: 10px;
+
    border: 1px solid #565656;
+
    border-collapse: collapse;
+
    vertical-align: text-top;
+
    }
+
 
+
/*Change the styling of table headers */
+
    #contentContainer th {
+
    background-color: #E8E8E9;
+
    padding: 10px;
+
    border: 1px solid #565656;
+
    border-collapse: collapse;
+
    vertical-align: text-top;
+
    }
+
 
+
 
+
 
+
/*MENU STYLING */
+
 
+
/*Styling for the links in the menu */
+
#menuContainer a {
+
color: #565656;
+
text-decoration:none;
+
font-weight: bold;
+
}
+
 
+
/* Sets the style for lists inside menuContainer  */
+
#menuContainer ul {
+
list-style: none;
+
margin-left:0px;
+
}
+
 
+
/*Styles the list items to become menu buttons */
+
#menuContainer ul li {
+
text-align: center;
+
display: block;
+
width: 100%;
+
height:30px;
+
padding-top:10px;
+
}
+
 
+
/*For the menu buttons, changes the color when hovering*/
+
#menuContainer li:hover {
+
color:  #FFF;
+
background-color: #24B694;
+
}
+
 
+
/*Submenus are not displayed as default*/
+
#menuContainer li ul {
+
display: none;
+
padding-top:15px;
+
margin-left: -19px;
+
}
+
 
+
/*Submenus are displayed when hovering the menu button */
+
#menuContainer li:hover ul {
+
/*display: inline-block; */
+
display: block;
+
position: absolute;
+
float:right;
+
margin-left: 134px;
+
margin-top:-42px;
+
}
+
 
+
/*Style the submenu buttons*/
+
#menuContainer li ul li{
+
background-color: #59BF92;
+
padding-left:20px;
+
padding-right:20px;
+
height:30px;
+
padding-top:10px;
+
margin-top:-2px;
+
color: #565656;
+
width: 150px;
+
}
+
 
+
/*CLASSES */
+
 
+
/*Clear class for all the pages, adds spacing too*/
+
.clear{
+
clear:both;
+
height: 10px;
+
}
+
 
+
 
+
/* highlight box for special messages */
+
    .highlightBox {
+
        width:500px;
+
        margin:auto;
+
        background-color: #E8E8E9;
+
margin-bottom: 15px;
+
margin-top: 15px;
+
padding: 15px;
+
padding-top: 5px;
+
    }
+
 
+
 
+
</style>
+
<!-- End of CSS -->
+
 
+
 
+
<!-- Start of the template html elements. -->
+
<div id ="mainContainer"><!--The closing tag for mainContainer should be placed at the bottom of each content page.-->
+
 
+
 
+
<div id="bannerContainer">
+
<br><br>
+
<h2> Add a banner to your wiki! </h2>
+
+
<p>You can make the image 980px  by  200px</p>
+
<p> Remember to call the file: "<i>Team_Chalmers-Gothenburg_banner.jpg</i>" </p>
+
</div>
+
 
+
<!-- Start of menu -->
+
<div id="menuContainer">
+
 
+
<!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg"><li>HOME</li></a>
+
 
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Team"><li>TEAM
+
<ul> 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Students"><li>Students</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Supervisors"><li>Supervisors</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>   
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Gallery"><li>Gallery</li></a>
+
+
</ul>
+
 
+
</li></a>
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project"><li>PROJECT
+
<ul> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Theory"><li>Theory</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Constructs"><li>Constructs</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Protocols"><li>Protocols</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/OtherApplications"><li>Other applications</li></a>
+
</ul>
+
</li></a>
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Achievements"><li>ACHIEVEMENTS
+
<ul> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project Results"><li>Project Results</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/BioBricks"><li>BioBricks</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Medals"><li>Medals</li></a> 
+
+
</ul>
+
</li></a>
+
 
+
 
+
<a href="#"><li>MODELING
+
            <ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Detection"><li>Detection</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety Switch"><li>Safety Switch</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Simulation"><li>Simulation</li></a> 
+
+
</ul>
+
</li></a>
+
+
 
+
 
+
 
+
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Practices"><li>HUMAN PRACTICES
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/iGEMCommunity"><li>iGEM Community</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/NextGeneration"><li>Next Generation</li></a> 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Society"><li>Society</li></a> 
+
+
</ul></a></li>
+
 
+
 
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Notebook"><li>NOTEBOOK
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Schedule"><li>Schedule</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>   
+
+
</ul>
+
</li></a>
+
     
+
+
 
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety"><li>SAFETY
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/LabSafety"><li>Lab Safety</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Ethics"><li>Ethics</li></a> 
+
+
</ul>
+
 
+
</li></a>
+
 
+
+
 
+
 
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Entrepreneurship"><li>THANKS
+
 
+
<ul>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Sponsors"><li>Sponsors</li></a>
+
<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Acknowledgements"><li>Acknowledgements</li></a> 
+
+
</ul>
+
 
+
</li></a>
+
 
+
</ul>
+
</div>
+
<!-- End of menu  -->
+
 
+
<!-- Start of content -->
+
<div id="contentContainer"> <!--The closing tag for contentContainer should be placed at the bottom of each content page.-->
+
</html>
+
 
+
 
+
<h1>BioBricks</h1>
+
 
+
<h2>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h2>
+
<p>Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from <i>STE2</i> (<i>Saccharomyces cerevisiae</i>) and Pheromone P-factor receptor <i>MAM2</i> (<i>Schizosaccharomyces pombe</i>) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from <i>S.pombe</i> through the Pheromone pathway in </i>S.cerevisiae</i>.</p>
+
 
+
<h2>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h2>
+
 
<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p>
 
<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p>
  
<h2>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h2>
+
<h3>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h3>
 
<p>Promoter to TPI1.</p>
 
<p>Promoter to TPI1.</p>
  
<h2>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h2>
+
<h3>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h3>
 
<p>Recombinase A from <i>Deinococcus radiodurans</i>. Used in DNA-repair mechanisms.  
 
<p>Recombinase A from <i>Deinococcus radiodurans</i>. Used in DNA-repair mechanisms.  
 
Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
 
Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
  
<h2>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h2>
+
<h3>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h3>
 
<p>Single strand binding protein from <i>Deinococcus radiodurans</i>. Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
 
<p>Single strand binding protein from <i>Deinococcus radiodurans</i>. Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>

Latest revision as of 03:33, 19 September 2015

BioBricks

1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2

Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from STE2 (Saccharomyces cerevisiae) and Pheromone P-factor receptor MAM2 (Schizosaccharomyces pombe) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from S.pombe through the Pheromone pathway in S.cerevisiae.

2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2

The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.

3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1

Promoter to TPI1.

4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA

Recombinase A from Deinococcus radiodurans. Used in DNA-repair mechanisms. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.

5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB

Single strand binding protein from Deinococcus radiodurans. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.