Difference between revisions of "Team:Minnesota/Modeling"

(Prototype team page)
 
 
(10 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
{{Minnesota}}
+
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
−
<html>
+
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<meta name="generator" content="MediaWiki 1.16.5" />
 +
<link rel="shortcut icon" href="/favicon.ico" />
 +
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2015.igem.org (en)" />
 +
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:Minnesota/Project/Insulin&amp;action=creativecommons" rel="meta" />
 +
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
 +
<link rel="alternate" type="application/atom+xml" title="2015.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:Minnesota/Project/Insulin - 2015.igem.org</title>
 +
<style type="text/css" media="screen, projection">/*<![CDATA[*/
 +
@import "/wiki/skins/common/shared.css?270";
 +
@import "/wiki/skins/igem/main.css?270";
 +
/*]]>*/</style>
 +
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
 +
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
 +
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
 +
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
 +
 +
<script>
 +
var skin="igem",
 +
stylepath="/wiki/skins",
 +
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
 +
wgArticlePath="/$1",
 +
wgScriptPath="/wiki",
 +
wgScriptExtension=".php",
 +
wgScript="/wiki/index.php",
 +
wgVariantArticlePath=false,
 +
wgActionPaths={},
 +
wgServer="https://2015.igem.org",
 +
wgCanonicalNamespace="",
 +
wgCanonicalSpecialPageName=false,
 +
wgNamespaceNumber=0,
 +
wgPageName="Team:Minnesota/Project/Insulin",
 +
wgTitle="Team:Minnesota/Project/Insulin",
 +
wgAction="view",
 +
wgArticleId=12505,
 +
wgIsArticle=true,
 +
wgUserName=null,
 +
wgUserGroups=null,
 +
wgUserLanguage="en",
 +
wgContentLanguage="en",
 +
wgBreakFrames=false,
 +
wgCurRevisionId=241211,
 +
wgVersion="1.16.5",
 +
wgEnableAPI=true,
 +
wgEnableWriteAPI=true,
 +
wgSeparatorTransformTable=["", ""],
 +
wgDigitTransformTable=["", ""],
 +
wgMainPageTitle="Main Page",
 +
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2015.igem.org", "5": "2015.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
 +
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2015.igem.org": 4, "2015.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
 +
wgSiteName="2015.igem.org",
 +
wgCategories=[],
 +
wgMWSuggestTemplate="https://2015.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
 +
wgDBname="2015_igem_org",
 +
wgSearchNamespaces=[0],
 +
wgMWSuggestMessages=["with suggestions", "no suggestions"],
 +
wgRestrictionEdit=[],
 +
wgRestrictionMove=[];
 +
</script>               
 +
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 +
<!-- Head Scripts -->
 +
<script src="/wiki/skins/common/ajax.js?270"></script>
 +
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
 +
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 +
<!-- jQuery Javascript -->
 +
<script type='text/javascript'        src ='/common/jquery-latest.min.js'></script>
 +
<script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
 +
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
 +
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
 +
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
 +
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
 +
</head>
  
−
<h2> Modeling</h2>
+
<body              class="mediawiki  ltr ns-0 ns-subject page-Team_Minnesota_Project_Insulin">
 +
  <div id="globalWrapper">
  
 +
    <div id="top-section">
 +
<div id="p-logo">
 +
    <a href="/Main_Page"
 +
      title="Main Page">
 +
    <img src='/wiki/skins/common/images/wiki.jpg'>"
 +
    </a>
 +
</div>  <!-- end p-logo -->
 +
<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
  
−
<div class="highlightBox">
 
−
<h4>Note</h4>
 
−
<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Model award</a>, you must fill out this page.</p>
 
−
</div>
 
  
  
−
<p>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.</p>
+
<div id="menubar" class='left-menu noprint'>
 +
  <ul>
 +
                  <li
 +
                class='selected'       ><a href="/Team:Minnesota/Project/Insulin">Page              </a></li>
 +
              <li
 +
                class='new'       ><a href="/wiki/index.php?title=Talk:Team:Minnesota/Project/Insulin&amp;action=edit&amp;redlink=1">Discussion              </a></li>
 +
              <li
 +
                    ><a href="/wiki/index.php?title=Team:Minnesota/Project/Insulin&amp;action=edit">View source              </a></li>
 +
              <li
 +
                    ><a href="/wiki/index.php?title=Team:Minnesota/Project/Insulin&amp;action=history">History              </a></li>
 +
              <li style='color:white;cursor:default'>teams</li>
 +
  </ul>
 +
</div> <!-- end menubar (left) -->
  
−
<p>
+
<div class="right-menu noprint" id="menubar">
−
Here are a few examples from previous teams:
+
    <ul>
−
</p>
+
                <li id="pt-login"><a href="/wiki/index.php?title=Special:UserLogin&amp;returnto=Team:Minnesota/Project/Insulin" title="You are encouraged to log in; however, it is not mandatory [o]" accesskey="o">Log in</a></li>     </ul>
 +
</div><!-- end right menubar -->
 +
 
 +
<div id="search-controls" class="noprint">
 +
<form action="/Special:Search" id="searchform">
 +
<input id="searchInput" name="search" type="text" title="Search 2015.igem.org [f]" accesskey="f" value="" />
 +
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;
 +
      <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
 +
</form>
 +
</div> <!-- close search controls -->
 +
    </div> <!-- close top-section-->
 +
    <div id="content">
 +
<a name="top" id="top"></a>
 +
<h1 class="firstHeading">Team:Minnesota/Project/Insulin</h1>
 +
<div id="bodyContent">
 +
<h3 id="siteSub" class='noprint'>From 2015.igem.org</h3>
 +
<div id="contentSub"></div>
 +
<!--
 +
<div id="jump-to-nav">Jump to:                        <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
 +
<!-- start content -->
 +
<p>
 +
<head>
 +
<title>Team:Minnesota - Main Style Template</title>
 +
 
 +
<style type="text/css">
 +
 
 +
/*========general scrolling div for maincontentbox=====*/
 +
.ScrollDiv{
 +
position:absolute;
 +
top:-10px;
 +
width:720px;
 +
height:450px;
 +
margin-left:10px;
 +
overflow-y:scroll;
 +
text-align:left;
 +
}
 +
 
 +
 
 +
/*==========special table code============*/
 +
table.ScrollList{
 +
position:absolute;
 +
width:700px;
 +
border: #787878 solid 2px;
 +
background-color:#A3FFA3;
 +
}
 +
 
 +
table.ScrollList tr{
 +
background-color:#B0B0B0;
 +
height:40px;
 +
}
 +
 
 +
table.ScrollList tr td{
 +
padding-left:10px;
 +
width:auto;
 +
}
 +
 
 +
table.ScrollList tr.header{
 +
padding-left:0px;
 +
height:30px;
 +
background-color:transparent;
 +
font-weight:bold;
 +
}
 +
table.ScrollList tr.header td{
 +
padding-left:0px;
 +
}
 +
 
 +
 
 +
 
 +
 
 +
 
 +
/*============Main Layout code=====================*/
 +
 
 +
 
 +
#ContentAll{
 +
position:absolute;
 +
width:1000px;
 +
height:700px;
 +
/*autocenter horizontally*/
 +
left:50%;
 +
margin-left:-500px;
 +
 
 +
background-color:transparent;
 +
 
 +
}
 +
 
 +
 
 +
#MainBanner{
 +
/*set to be flush against content*/
 +
position:absolute;
 +
top:0px;
 +
left:0px;
 +
margin:0px;
 +
padding:0px;
 +
width:1000px;
 +
height:150px;
 +
background-color:#660000;//#7b111b;
 +
opacity:0.6;
 +
z-index:-1;
 +
}
 +
 
 +
#MainBannerImage{//outside and on top of main banner to allow for escape from opacity
 +
/*set to be flush against content*/
 +
position:absolute;
 +
top:0px;
 +
left:0px;
 +
margin:0px;
 +
padding:0px;
 +
width:1000px;
 +
height:150px;
 +
background-color:transparent;
 +
opacity:1.0;
 +
z-index:2;
 +
}
 +
 
 +
 
 +
#SideBarAll{
 +
/*set to be flush against left and MainBanner*/
 +
position:absolute;
 +
top:150px;
 +
left:0px;
 +
width:200px;
 +
height:550px;
 +
background-color:white;
 +
}
 +
 
 +
#MainBoxAll{
 +
/*set to be flush against MainBanner and SideBarAll*/
 +
position:absolute;
 +
top:150px;
 +
left:200px;
 +
width: 800px;
 +
height:550px;
 +
background-color:white;
 +
}
 +
 
 +
#MainBoxContent{
 +
position:absolute;
 +
width:750px;
 +
height:450px;
 +
background-color:gray;
 +
left:25px;
 +
top:25px;
 +
}
 +
 
 +
#MainBoxOtherLinks{
 +
position:absolute;
 +
width: 800px;
 +
height: 75px;
 +
background-color: white;
 +
top: 475px;
 +
}
 +
 
 +
#MainBoxOtherLinks p.otherLinks{
 +
position:absolute;
 +
left:100px;
 +
top:25px;
 +
background-color: none;
 +
color: gray;
 +
 
 +
}
 +
 
 +
#MainBoxOtherLinks p.otherLinks a{
 +
  text-decoration:none;
 +
  color: gray;
 +
}
 +
 
 +
#MainBoxOtherLinks p.otherLinks a:hover{
 +
  text-decoration:underline;
 +
}
 +
 
 +
 
 +
body{
 +
background-image:url("https://static.igem.org/mediawiki/2015/9/9d/MN_2015_BG.png");
 +
background-color:gray;
 +
}
 +
 
 +
/*end container boxs*/
 +
 
 +
/*Start Revised sidebar styles*/
 +
 
 +
.sideNavBar2
 +
{
 +
position:absolute;
 +
top:25px;
 +
left:0px;
 +
margin:0;
 +
padding:0;
 +
border:0px;
 +
}
 +
 
 +
.sideNavBar2 ul li
 +
{
 +
list-style:none;
 +
margin:0;
 +
padding:0;
 +
}
 +
 
 +
.sideNavBar2 a{
 +
    font-family: Trebuchet MS, Verdana, Century Gothic, Tahoma, sans-serif;
 +
    font-size:20px;
 +
    //font-weight:600;
 +
 
 +
    padding-top:5px;
 +
    padding-left:15px;
 +
 
 +
 
 +
    text-decoration:none;
 +
    background-color:white;
 +
    display:block;
 +
    width:150px;
 +
    height:35px;
 +
}
 +
 
 +
.sideNavBar2 a:link {
 +
    border-width:1px 1px 1px 3px;
 +
    border-style:none none none solid;
 +
    border-color:white;
 +
    color: gray;
 +
 
 +
}
 +
 
 +
.sideNavBar2 a:visited {
 +
    border-width:1px 1px 1px 3px;
 +
    border-style:none none none solid;
 +
    border-color:white;
 +
    color: gray;
 +
}
 +
 
 +
.sideNavBar2 a:hover { /*cool button*/
 +
    border-width:1px 1px 1px 3px;
 +
    border-style: none none none solid;
 +
    border-color:#660000 #660000 #660000 #660000;
 +
    color: #FBB917;
 +
}
 +
.sideNavBar2 a:active {
 +
    border-width:1px 1px 1px 3px;
 +
    border-style: none none none solid;
 +
    border-color:#660000 #660000 #660000 #660000;
 +
    color: #FBB917;
 +
}
 +
 
 +
.sideNavBar2 a.current{ /*force black for current button*/
 +
    color: #660000;
 +
}
 +
 
 +
.sideNavBar2 a.current:hover{ /*allow for gold to still happen for current button*/
 +
    color: #FBB917;
 +
}
 +
 
 +
.sideSocialMediaButtons{
 +
position:absolute;
 +
width:120px;
 +
height:60px;
 +
top:460px;
 +
left:30px;
 +
background-color:white;
 +
}
 +
 
 +
 
 +
/*End Revised sidebar styles*/
 +
 
 +
/*Start Main Content items*/
 +
.rightNavButtons {
 +
position:absolute;
 +
top:140px;
 +
left:695px;
 +
width:80px;
 +
height:220px;
 +
background-color:transparent;
 +
}
 +
 
 +
.rightNavButtons a{
 +
display:block;
 +
}
 +
 
 +
.mainContentTextBanner{
 +
position:absolute;
 +
top:350px;
 +
left:0px;
 +
width:730px;
 +
height:80px;
 +
background-color:#660000;
 +
opacity:0.7;
 +
font-size:20px;
 +
color:white;
 +
padding: 10px;
 +
}
 +
 
 +
/*============the following section is hacking the original wiki template============*/
 +
 
 +
#contentSub, #search-controls, .firstHeading, #footer-box, #catlinks, #p-logo {
 +
    display:none;
 +
}
 +
 
 +
#top-section {
 +
    border: none;
 +
    height: 0px;
 +
}
 +
 
 +
#content {
 +
    border: none;
 +
    background-color: transparent;
 +
}
 +
 
 +
 
 +
#menubar > ul > li:last-child {
 +
    display: none;
 +
}
 +
 
 +
#menubar { //resizes menu bar
 +
    width: auto;
 +
}
 +
 
 +
/*hacks menubar links to do cool things*/
 +
#menubar a:link {color:transparent;}
 +
#menubar a:visited {color:transparent;}
 +
#menubar a:hover {color:black;}
 +
#menubar a:active {color:gray;}
 +
 
 +
 
 +
#top-section { /*section with the buttons and search bar - might be bad to remove*/
 +
    border: 0px transparent solid;
 +
    position:absolute;
 +
    left:140px;
 +
    /*display:none;*/
 +
}
 +
 
 +
#menubar { /*main menubar settings*/
 +
    background-color: transparent;
 +
}
 +
#menubar ul li a { /*menubar subcategory items*/
 +
    color: black;
 +
}
 +
 
 +
.right-menu li a {
 +
    color: black;
 +
    background-color: transparent;
 +
}
 +
 
 +
/*============End original wiki mods============*/
 +
 
 +
.mainBox{
 +
position: absolute;
 +
top: 375px;
 +
left: 210px;
 +
width:700px;
 +
height:300px;
 +
text-align:center;
 +
}
 +
 
 +
 
 +
.mainBox td{
 +
  align:center;
 +
  height:130px;
 +
  padding:10px; /*between border &amp; content&amp;*/
 +
  border:solid #E0E0E0 1px;
 +
}
 +
.mainBox td.profpic{
 +
  width:150px;
 +
  padding:0px; /*between border &amp; content&amp;*/
 +
}
 +
 
 +
 
 +
p.filler
 +
{
 +
position:relative;
 +
padding-top:100px;
 +
font-family:arial;
 +
color:gray;
 +
font-size:20px;
 +
padding:50px;
 +
}
 +
 
 +
body
 +
{
 +
    background-color:transparent;
 +
    margin:0;
 +
    padding:0;
 +
}
 +
 
 +
table,td
 +
{
 +
    border:0px;
 +
}
 +
 
 +
/*=========Start side nav bar code==============*/
 +
.sideNavBar
 +
{
 +
position:absolute;
 +
top:320px;
 +
left:0px;
 +
background-color:white;
 +
margin:0;
 +
padding:0;
 +
border:0px;
 +
}
 +
 
 +
.sideNavBar ul li
 +
{
 +
list-style:none;
 +
margin:0;
 +
padding:0;
 +
}
 +
 
 +
.sideNavBar a{
 +
    font-family: Verdana, Century Gothic, Tahoma, sans-serif;
 +
    font-size:20px;
 +
    /*font-weight:600;*/
 +
    padding-top:15px;
 +
    padding-left:30px;
 +
    text-decoration:none;
 +
    background-color:white;
 +
    display:block;
 +
    width:170px;
 +
    height:45px;
 +
}
 +
 
 +
.sideNavBar a:link {
 +
    border-width:1px 1px 1px 3px;
 +
    border-style:none none none solid;
 +
    border-color:white;
 +
    color: gray;
 +
 
 +
}
 +
 
 +
.sideNavBar a:visited {
 +
    border-width:1px 1px 1px 3px;
 +
    border-style:none none none solid;
 +
    border-color:white;
 +
    color: gray;
 +
}
 +
 
 +
.sideNavBar a:hover { /*cool button*/
 +
    border-width:1px 1px 1px 3px;
 +
    border-style: none none none solid;
 +
    border-color:#660000 #660000 #660000 #660000;
 +
    color: gold;
 +
}
 +
.sideNavBar a:active {
 +
    border-width:1px 1px 1px 3px;
 +
    border-style: none none none solid;
 +
    border-color:#660000 #660000 #660000 #660000;
 +
    color: gold;
 +
}
 +
 
 +
.sideNavBar a.current{ /*force black for current button*/
 +
    color: black;
 +
}
 +
 
 +
.sideNavBar a.current:hover{ /*allow for gold to still happen for current button*/
 +
    color: #660000;
 +
}
 +
 
 +
/*=============End side nav bar code=================*/
 +
 
 +
/*=============Start top nav bar code===============*/
 +
.insideTopBar {
 +
    position:absolute;
 +
    top:300px;
 +
    left:230px;
 +
    background-color:white;
 +
 
 +
}
 +
.insideTopBar ul{
 +
    padding:0;
 +
    margin:0;
 +
    list-style:none;
 +
}
 +
.insideTopBar ul li{
 +
    float:right;
 +
    padding-left:7px;
 +
    margin:0px;
 +
 
 +
}
 +
 
 +
.insideTopBar a{
 +
 
 +
    display:block;
 +
    background-color:white;
 +
    text-decoration:none;
 +
}
 +
 
 +
.insideTopBar img{
 +
    opacity:0.4;
 +
    filter:alpha(opacity=40); /* For IE8 and earlier */
 +
}
 +
.insideTopBar img:hover{
 +
    opacity:1.0;
 +
    filter:alpha(opacity=100); /* For IE8 and earlier */
 +
}
 +
 
 +
/*=============End top nav bar code=================*/
 +
 
 +
 
 +
/*====I hate doing this, but this is a GREAT CSS photo gallery...==*/
 +
/* ================================================================
 +
This copyright notice must be untouched at all times.
 +
 
 +
The original version of this stylesheet and the associated (x)html
 +
is available at http://www.cssplay.co.uk/menu/lightbox.html
 +
Copyright (c) 2005-2007 Stu Nicholls. All rights reserved.
 +
This stylesheet and the associated (x)html may be modified in any
 +
way to fit your requirements.
 +
=================================================================== */
 +
 
 +
/* slides styling */
 +
 
 +
.photo {width:635px; text-align:left; position:relative; margin:0 auto;}
 +
 
 +
.photo ul.topic {padding:0; margin:0; list-style:none; width:635px; height:auto; position:relative; z-index:10;}
 +
 
 +
.photo ul.topic li {display:block; width:125px; height:31px; float:left;}
 +
.photo ul.topic li a.set {display:block; font-size:11px; width:124px; height:30px; text-align:center; line-height:30px; color:#000; text-decoration:none; border:1px solid #fff; border-width:1px 1px 0 0; background:#ccc; font-family:verdana, arial, sans-serif;}
 +
 
 +
.photo ul.topic li a ul,
 +
.photo ul.topic li ul
 +
{display:none;}
 +
 
 +
.photo ul.topic li.active a
 +
{color:#000; background:#bbb;}
 +
 
 +
.photo ul.topic li a:hover,
 +
.photo ul.topic li:hover a
 +
{color:#fff; background:#aaa;}
 +
 
 +
.photo ul.topic li.active ul
 +
{display:block; position:absolute; left:0; top:31px; list-style:none; padding:0; margin:0; height:375px; background:#ddd; width:464px; padding:40px 60px; border:20px solid #bbb; z-index:1;}
 +
 
 +
.photo ul.topic li a:hover ul,
 +
.photo ul.topic li:hover ul
 +
{display:block; position:absolute; left:0; top:31px; list-style:none; padding:0; margin:0; height:375px; background:#ddd; width:464px; padding:40px 60px; border:20px solid #aaa; z-index:100;}
 +
 
 +
.photo ul.topic li ul li
 +
{display:inline; width:112px; height:87px; float:left; border:1px solid #fff; margin:1px;}
 +
 
 +
.photo ul.topic li ul li a
 +
{display:block; width:110px; height:85px; cursor:default; float:left; text-decoration:none; background:#444; border:1px solid #888;}
 +
 
 +
.photo ul.topic li ul li a img
 +
{display:block; width:100px; height:75px; border:5px solid #eee;}
 +
 
 +
.photo ul.topic li a:hover ul li a:hover,
 +
.photo ul.topic li:hover ul li a:hover
 +
{white-space:normal; position:relative;}
 +
 
 +
.photo ul.topic li a:hover ul li a:hover img,
 +
.photo ul.topic li:hover ul li a:hover img
 +
{position:absolute; left:-50px; top:-32px; width:200px; height:150px; border-color:#fff;}
 +
 
 +
 
 +
 
 +
</style>
 +
</head>
 +
<body>
 +
 
 +
 
 +
</body>
 +
 
 +
 
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<title>Team:Minnesota - Template</title>
 +
 
 +
<script type="text/javascript" src="jquery.js"></script>
 +
<script type="text/javascript">
 +
 
 +
 
 +
$(document).ready(function(){
 +
 
 +
  // jQuery functions go here...
 +
 
 +
//vertical autocenter
 +
$(window).resize(function(){
 +
  $('#ContentAll').css({
 +
    position:'absolute',
 +
    top: ($(window).height() - $('#ContentAll').outerHeight())/2
 +
  });
 +
  $('#bgImageDiv').css({
 +
    position:'absolute',
 +
    top: -1*($(window).height() - $('#bgImageDiv').height())/2
 +
  });
 +
 
 +
});
 +
 
 +
$(window).resize();
 +
//end vertial autocenter
 +
 
 +
//background image centering
 +
 
 +
//main page image switcher
 +
 
 +
 
 +
 
 +
$("#mainRightButton2").hover(
 +
    function() {
 +
      $(this).attr("src","http://i1158.photobucket.com/albums/p607/iGEM_MN/caffeinatedbread_gold_txt.png");},         
 +
    function() {$(this).attr("src","http://i1158.photobucket.com/albums/p607/iGEM_MN/caffeinatedbread_gold_img.png");}
 +
);
 +
 
 +
$("#mainRightButton3").hover(
 +
    function() {
 +
      $(this).attr("src","http://i1158.photobucket.com/albums/p607/iGEM_MN/bacterialsunscreen_maroon_txt.png");},         
 +
    function() {$(this).attr("src","http://i1158.photobucket.com/albums/p607/iGEM_MN/bacterialsunscreen_maroon_img.png");}
 +
);
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
}); //ends .ready function
 +
 +
 
 +
 
 +
</script>
 +
 
 +
<style type="text/css">
 +
 
 +
 
 +
</style>
 +
 
 +
 
 +
</head>
 +
 
 +
 
 +
<body>
 +
 
 +
 
 +
<div id="ContentAll">
 +
 
 +
<div id="ContentAll">
 +
 
 +
<div id="MainBannerImage">
 +
<img src="https://static.igem.org/mediawiki/2015/b/bc/MN_2015_top_banner.png">
 +
</div>
 +
<div id="MainBanner">
 +
</div>
 +
 
 +
 
 +
<div id="SideBarAll">
 +
<div class="sideNavBar2">
 
<ul>
 
<ul>
−
<li><a href="https://2014.igem.org/Team:ETH_Zurich/modeling/overview">ETH Zurich 2014</a></li>
+
<li><a href='https://2015.igem.org/Team:Minnesota'>HOME</a></li>
−
<li><a href="https://2014.igem.org/Team:Waterloo/Math_Book">Waterloo 2014</a></li>
+
<li><a href='https://2015.igem.org/Team:Minnesota/Team'>TEAM</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Project'>PROJECT</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Software'>SOFTWARE</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Practices'>PRACTICES</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Notebook'>NOTEBOOK</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Attributions'>ATTRIBUTIONS</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Safety'>SAFETY</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Sponsor'>SPONSOR</a></li>
 
</ul>
 
</ul>
 +
</div>
 +
 +
 +
</div> <!--End sideBarAll-->
 +
 +
<div id="MainBoxAll">
 +
 +
<div id="MainBoxContent" style="background-color:white;">
 +
 +
<div style="position:absolute;top:0px;margin-left:50px; width:600px; height:450px; overflow-y:scroll; text-align:left; overflow-x:hidden;">
 +
<!---START HERE--->
 +
 +
 +
<h1> 2A Viral Tag Modeling for Biosynthesis </h1><br>
 +
 +
<i> &nbsp; &nbsp; &nbsp; &nbsp;  Building a 2A eukaryotic biochemical network can be hard, and these viral tags are not without a drawback. Previous research (Beekwilder 2014) has shown there was a decreased level of expression of enzymes downstream of the promoter. This can certainly affect the overall yield of the pathway, but we can certainly minimize this effect!
 +
</i><br><br>
 +
 +
 +
<br>
 +
&nbsp; &nbsp; &nbsp; &nbsp; We have designed a computation tool to combinatorically test all available rearrangements of your biochemical network and display the ideal ordering of your network to optimize reaction efficiency. The model utilizes the heavily studied kinetics of enzymes and implements their behavior in a differential equations model. This is nested within a permutation loop to test all possible orderings.
 +
<br><br>
 +
<br>
 +
 +
<center><i> To prove how easy it is to use, we even redesigned three 2014 iGEM teams circuits for how they would be optimally arranged in eukaryotic 2A systems! </center></i><br><br>
 +
 +
 +
 +
<center> <b> 2014 Arizona State Pyruvate to Fatty Acid Ethyl Esters </b></center><br>
 +
 +
<center> Promoter -> pyruvate dehydrogenase (1st)-> Long-chain acyl-ACP reductase (2nd) -> Alcohol dehydrogenase (3rd) -> Long-chain-fatty-acid-CoA ligase EC (4th) </center><br><br>
 +
 +
                             
 +
 +
<center> <b> 2014 BYU Denitrification </b></center><br>
 +
 +
<center> Promoter -> Nitrite reductase (1st) -> Nitrous-oxide reductase (2nd) -> Nitric Oxide reductase (3rd) ></center><br><br>
 +
 +
 +
 +
<center> <b> 2014UIUC caffeine degradation for dogs </b></center><br>
 +
 +
Promoter -> 7-methylxanthine demethylase (1st)-> Methylxanthine N1-demethylase (2nd) -> Methylxanthine N1-demethylase (3rd) ></center><br><br><br>
 +
 +
&nbsp; &nbsp; &nbsp; &nbsp; To emphasize the portability of this tool, the system was designed in MATLAB and embedded in a graphical user interface (GUI). If you want to test this tool out or use it to optimize your own experiments, head on over to:
 +
 +
 +
<br><br><center>https://github.com/PatrickHolec/Pathway2A</center><br>
 +
<center>or contact:</center><br>
 +
<center>Patrick V. Holec</center>
 +
<center>hole0077@umn.edu</center>
 +
<center>University of Minnesota</center><br>
 +
 +
<img src="https://static.igem.org/mediawiki/2015/f/ff/Model_1.png" width=96% height=86%>
 +
<br>
 +
 +
<!---END-->
 +
 +
</div>
 +
 +
</div> <!--end mainboxcontent-->
 +
 +
<div class="rightNavButtons">
 +
 +
 +
 +
</div><!--end rightNavButtons-->
 +
 +
 +
<div id="MainBoxOtherLinks">
 +
<p class="otherLinks">
 +
<center><a href="https://2015.igem.org/Main_Page">iGEM Home</a> | <a href="https://igem.org/2015_Judging_Form?id=1626">iGEM Judge-Click Here!</a> | <a href="https://igem.org/Team.cgi?id=1626">Team Minnesota Info</a> |
 +
<a href="https://twin-cities.umn.edu/">University of Minnesota</a> | <a href="https://2015.igem.org/Team:Minnesota/Contact">Contact Us!</a></center>
 +
</p>
 +
 +
</div> <!--end mainboxotherlinks-->
 +
 +
 +
 +
</div> <!--end mainboxall-->
 +
 +
 +
</div> <!--end content all-->
 +
 +
 +
 +
 +
 +
<div class="hidden">
 +
<script type="text/javascript">
 +
<!--//--><![CDATA[//><!--
 +
var images = new Array()
 +
function preload() {
 +
for (i = 0; i < preload.arguments.length; i++) {
 +
images[i] = new Image()
 +
images[i].src = preload.arguments[i]
 +
}
 +
}
 +
preload(
 +
"http://i1158.photobucket.com/albums/p607/iGEM_MN/image4_resized.png",
 +
 +
)
 +
//--><!]]>
 +
</script>
 +
</div>
 +
</body>
 +
 +
 +
 +
 +
</p>
 +
<!--
 +
NewPP limit report
 +
Preprocessor node count: 9/1000000
 +
Post-expand include size: 41/2097152 bytes
 +
Template argument size: 0/2097152 bytes
 +
Expensive parser function count: 0/100
 +
-->
 +
 +
<!-- Saved in parser cache with key 2015_igem_org:pcache:idhash:12505-0!1!0!!en!2!edit=0 and timestamp 20150917043130 -->
 +
<div class="printfooter">
 +
Retrieved from "<a href="C:\Documents and Settings\aj\Desktop\IGEM webpage/Policy And Practices.html">C:\Documents and Settings\aj\Desktop\IGEM webpage/Policy And Practices.html</a>"</div>
 +
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
 +
<div class="visualClear"></div>
 +
</div>
 +
    </div>
 +
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
 +
<div class="visualClear"></div>
 +
    <div id='footer-box' class='noprint'>
 +
        <div id="footer">
 +
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
 +
 +
 +
  <!-- Recentchanges is not handles well DEBUG -->
 +
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
 +
      title='Recent changes'>Recent changes</a></li>
 +
 +
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Minnesota/Project/Insulin"
 +
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
 +
 +
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Minnesota/Project/Insulin"
 +
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
 +
 +
 +
 +
                <li id="t-specialpages"><a href="/Special:SpecialPages"
 +
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
 +
                </li>
 +
                <li><a href='/Special:Preferences'>My preferences</a></li>
 +
            </ul>
 +
        </div> <!-- close footer -->
 +
        <div id='footer'>
 +
    <ul id="f-list">
 +
 +
            <li id="t-print"><a href="/wiki/index.php?title=Team:Minnesota/Project/Insulin&amp;printable=yes"
 +
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
 +
            </li>
 +
 +
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:Minnesota/Project/Insulin&amp;oldid=241211"
 +
              title="Permanent link to this revision of the page">Permanent link</a>
 +
            </li>
  
  
 +
        <li id="privacy"><a href="/2015.igem.org:Privacy_policy" title="2015.igem.org:Privacy policy">Privacy policy</a></li>
 +
        <li id="disclaimer"><a href="/2015.igem.org:General_disclaimer" title="2015.igem.org:General disclaimer">Disclaimers</a></li>
 +
    </ul>
 +
</div> <!-- close footer -->
 +
    </div> <!-- close footer-box -->
 +
 +
<script>if (window.runOnloadHook) runOnloadHook();</script>
 
</div>
 
</div>
 +
<!-- Served in 0.190 secs. --></body>
  
 
</html>
 
</html>

Latest revision as of 03:52, 19 September 2015

Team:Minnesota/Project/Insulin - 2015.igem.org

 

Team:Minnesota/Project/Insulin

From 2015.igem.org

Team:Minnesota - Main Style Template Team:Minnesota - Template

2A Viral Tag Modeling for Biosynthesis


        Building a 2A eukaryotic biochemical network can be hard, and these viral tags are not without a drawback. Previous research (Beekwilder 2014) has shown there was a decreased level of expression of enzymes downstream of the promoter. This can certainly affect the overall yield of the pathway, but we can certainly minimize this effect!


        We have designed a computation tool to combinatorically test all available rearrangements of your biochemical network and display the ideal ordering of your network to optimize reaction efficiency. The model utilizes the heavily studied kinetics of enzymes and implements their behavior in a differential equations model. This is nested within a permutation loop to test all possible orderings.


To prove how easy it is to use, we even redesigned three 2014 iGEM teams circuits for how they would be optimally arranged in eukaryotic 2A systems!


2014 Arizona State Pyruvate to Fatty Acid Ethyl Esters

Promoter -> pyruvate dehydrogenase (1st)-> Long-chain acyl-ACP reductase (2nd) -> Alcohol dehydrogenase (3rd) -> Long-chain-fatty-acid-CoA ligase EC (4th)


2014 BYU Denitrification

Promoter -> Nitrite reductase (1st) -> Nitrous-oxide reductase (2nd) -> Nitric Oxide reductase (3rd) >


2014UIUC caffeine degradation for dogs

Promoter -> 7-methylxanthine demethylase (1st)-> Methylxanthine N1-demethylase (2nd) -> Methylxanthine N1-demethylase (3rd) >


        To emphasize the portability of this tool, the system was designed in MATLAB and embedded in a graphical user interface (GUI). If you want to test this tool out or use it to optimize your own experiments, head on over to:

https://github.com/PatrickHolec/Pathway2A

or contact:

Patrick V. Holec
hole0077@umn.edu
University of Minnesota


Retrieved from "C:\Documents and Settings\aj\Desktop\IGEM webpage/Policy And Practices.html"