Difference between revisions of "Team:Uppsala/Results"
| Line 94: | Line 94: | ||
| − | + | <!-- <h2 id="enz_deg">Enzymatic degradation</h2> | |
<hr> | <hr> | ||
<u><b><p>Restriction free cloning (RFC)</p></b></u> | <u><b><p>Restriction free cloning (RFC)</p></b></u> | ||
| Line 613: | Line 613: | ||
<p> | <p> | ||
The drop collapse test, CTAB test, TLC and mass spectrometry showed positive result and we could confirm that mono-rhamnolipids are expressed by our construct (<a href="http://parts.igem.org/Part:BBa_K1688000"><span class="res_link">BBa_K1688000</span></a>) with <i>E.coli</i> BL21DE3. However, we still need to study their expression in the presence of PAH degrading enzymes (dioxygenase and laccase) and PAHs, to know whether these may influence the mono-rhamnolipid synthesis. Our future plan is that biosurfactant strains will be used together with the strains that expresses the PAH degrading enzymes. The biosurfactants will break down the clustered PAHs and make them available to degrading enzymes for an efficient degradation. | The drop collapse test, CTAB test, TLC and mass spectrometry showed positive result and we could confirm that mono-rhamnolipids are expressed by our construct (<a href="http://parts.igem.org/Part:BBa_K1688000"><span class="res_link">BBa_K1688000</span></a>) with <i>E.coli</i> BL21DE3. However, we still need to study their expression in the presence of PAH degrading enzymes (dioxygenase and laccase) and PAHs, to know whether these may influence the mono-rhamnolipid synthesis. Our future plan is that biosurfactant strains will be used together with the strains that expresses the PAH degrading enzymes. The biosurfactants will break down the clustered PAHs and make them available to degrading enzymes for an efficient degradation. | ||
| − | </p> | + | </p>--> |
</div> | </div> | ||
Latest revision as of 03:55, 19 September 2015
Results
Please click on the links above to view the results for the different parts of our project.