Difference between revisions of "Team:Penn/Communication"

 
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    <p>When the New York fashion community notices your brand, the world soon follows. The widespread love for UGG extended to Europe in the mid-2000's along with the stylish casual movement and demand for premium casual fashion. UGG boots and shoes were now seen walking the streets of London, Paris and Amsterdam with regularity. To meet the rising demand from new fans, UGG opened flagship stores in the UK and an additional location in Moscow. As the love spread farther East, concept stores were opened in Beijing, Shanghai and Tokyo. UGG Australia is now an international brand that is loved by all. This love is a result of a magical combination of the amazing functional benefits of sheepskin and the heightened emotional feeling you get when you slip them on your feet. In short, you just feel better all over when you wear UGG boots, slippers, and shoes.</p>
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  <p><br>Up to this point, all the data collected, especially the approximate luminescence intensity of our sender made with the conversion sequence, suggested the luminescence of sender cultures would be sufficient for successful activation of receiver circuit. The next step was to demonstrate successful sender-receiver communication.</p>
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    <p class="margin-top-10">In 2011, UGG will go back to its roots and focus on bringing the active men that brought the brand to life back with new styles allowing them to love the brand again as well. Partnering with Super Bowl champion and NFL MVP Tom Brady, UGG will invite even more men to feel the love the rest of the world knows so well. UGG will also step into the world of high fashion with UGG Collection. The UGG Collection fuses the timeless craft of Italian shoemaking with the reliable magic of sheepskin, bringing the luxurious feel of UGG to high end fashion. As the love for UGG continues to spread across the world, we have continued to offer new and unexpected ways to experience the brand. The UGG journey continues on and the love for UGG continues to spread.</p>
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<p><br>The figure above shows the arrangement of the sender and receiver in this experiment. RFP fluorescence, luminescence, and O.D. at 600 nm was measured every 2 hours. With three trials per strain, the following data was procured: </p>
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<p><br>As suggested in our predictions, the receiver was induced by the bioluminescent signal from the sender. Looking at pDawn activation through RFP expression, SY104 was the strain which resulted in the most receiver activation over time. This observation falls in line with the team’s prediction before that a sender with sustained expression light-production will have a greater effect on a receiver which requires a sustained input, like pDawn, than a sender with a transient luminescence output. </p>
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<p class="margin-top-10"><br><b> NOT DONE YET…</b> </p>
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<p><br>Even though we demonstrated successful communication between sender and receiver in our proposed system, our results do not show a completely reliable communication system. We compared our senders approximated intensity output (8 uW/cm^2) to the previously recorded intensity for saturation of pDawn (around 14 uW/cm^2) (Ohlendorf R. et. al. 2010).  We are not reaching the saturation point with our sender culture yet. However, we are close. There is approximately a three-fold difference between our intensity and the proposed saturated intensity. Moving forward, it would be imperative to improve some parts of our circuits to diminish this three-fold difference. </p>
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<p><br>The addition of nonanal (a carbohydrate that serves as a substrate for the luciferase reaction) was shown to increased the luminescence output by Yagur-Kroll and Belkin (Yagur-Kroll and Belkin, 2010). Though it showed a three-fold difference for certain strains, it reduced OD600 readings. However, as we know uncover more familiar about light-sensitive circuits such as pDawn and the mechanism of luminescent genes in different strains, we hope to tune our system (e.g. swap a promoter, switch the receiver, etc.) to yield a sender that reliably activates the receiver to saturation. </p>
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Latest revision as of 03:56, 19 September 2015

University of Pennsylvania iGEM

PENN iGEM 2015



LIGHT BASED COMMUNICATION


Up to this point, all the data collected, especially the approximate luminescence intensity of our sender made with the conversion sequence, suggested the luminescence of sender cultures would be sufficient for successful activation of receiver circuit. The next step was to demonstrate successful sender-receiver communication.


The figure above shows the arrangement of the sender and receiver in this experiment. RFP fluorescence, luminescence, and O.D. at 600 nm was measured every 2 hours. With three trials per strain, the following data was procured:


As suggested in our predictions, the receiver was induced by the bioluminescent signal from the sender. Looking at pDawn activation through RFP expression, SY104 was the strain which resulted in the most receiver activation over time. This observation falls in line with the team’s prediction before that a sender with sustained expression light-production will have a greater effect on a receiver which requires a sustained input, like pDawn, than a sender with a transient luminescence output.


NOT DONE YET…


Even though we demonstrated successful communication between sender and receiver in our proposed system, our results do not show a completely reliable communication system. We compared our senders approximated intensity output (8 uW/cm^2) to the previously recorded intensity for saturation of pDawn (around 14 uW/cm^2) (Ohlendorf R. et. al. 2010). We are not reaching the saturation point with our sender culture yet. However, we are close. There is approximately a three-fold difference between our intensity and the proposed saturated intensity. Moving forward, it would be imperative to improve some parts of our circuits to diminish this three-fold difference.


The addition of nonanal (a carbohydrate that serves as a substrate for the luciferase reaction) was shown to increased the luminescence output by Yagur-Kroll and Belkin (Yagur-Kroll and Belkin, 2010). Though it showed a three-fold difference for certain strains, it reduced OD600 readings. However, as we know uncover more familiar about light-sensitive circuits such as pDawn and the mechanism of luminescent genes in different strains, we hope to tune our system (e.g. swap a promoter, switch the receiver, etc.) to yield a sender that reliably activates the receiver to saturation.