Difference between revisions of "Team:BostonU"

 
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<li><a href="https://2014.igem.org/Team:BostonU/Team">TEAM</a>
 
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<li><a href="">PROJECT</a>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:BostonU/Chimera">Overview</a></li>
 
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<li><a href="https://2014.igem.org/Team:BostonU/Encoder">Priority Encoder</a></li>
 
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<li><a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo Assembly Method</a></li>
 
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                        <li><a href="https://2014.igem.org/Team:BostonU/Backbones">Low Copy Backbones</a></li>
 
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<li><a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">Tandem Promoters</a></li>
 
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      <li><a href="https://2014.igem.org/Team:BostonU/FusionProteins">Fusion Proteins</a></li>
 
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      <li><a href="https://2014.igem.org/Team:BostonU/Repressors">Repressor Proteins</a></li>
 
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            <li><a href="https://2014.igem.org/Team:BostonU/Multiplexing">Multiplexing</a></li>
 
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<li><a href="https://2014.igem.org/Team:BostonU/Software">BioDesign Automation Tools</a></li>
 
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            <li><a href="https://2014.igem.org/Team:BostonU/Future">Future Work</a></li>
 
  
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<li><a href="">ACHIEVEMENTS</a>
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<h2 style="font-color:#000000; margin-left:50px;">Developing conditionally dimerizable split protein systems for genetic logic and genome editing applications </h2>
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        <li><a href="https://2014.igem.org/Team:BostonU/Data">Data Collected</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
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            <li><a href="https://2014.igem.org/Team:BostonU/Workflow">Chimera Workflow</a></li>
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            <li><a href="https://2014.igem.org/Team:BostonU/ChimeraExample">Chimera Example</a></li>
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            <li><a href="https://2014.igem.org/Team:BostonU/Medals">Medal Fulfillment</a></li>
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<p style="padding-bottom:50px;">The field of synthetic biology seeks to engineer desirable cellular functionalities by developing molecular technologies that enable precise genetic manipulation. A promising solution is to reliably control proteins that naturally execute genetic modifications. Current strategies to regulate activity of such proteins primarily rely on modulating protein expression level through transcriptional control; however, these methods are susceptible to slow response and leaky expression. In contrast, strategies that exploit post-translational regulation of activity, such as conditional dimerization of split protein halves, have been demonstrated to bypass these limitations. Here, we compare the relative efficiency of previously characterized dimerization domains in regulating activities of three important genetic manipulation proteins - integrases and recombination directionality factors for genetic logic applications, and saCas9 for in vivo genome editing applications. We also establish guidelines to rationally identify promising protein split sites. Our characterization of these systems in mammalian cells ultimately paves way for important biomedical applications.</p>
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<li><a href="">NOTEBOOK</a>
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      <li><a href="https://2014.igem.org/Team:BostonU/Training">Training</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Protocols">Protocols</a></li>
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                        <li><a href="https://2014.igem.org/Team:BostonU/BackbonesNotebook">Backbones</a></li>
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                        <li><a href="https://2014.igem.org/Team:BostonU/TandemPromoters">Tandem Promoters and Repressors</a></li>
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                        <li><a href="https://2014.igem.org/Team:BostonU/FusionProteinsNotebook">Fusion Proteins</a></li>
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<li><a href="">CONSIDERATIONS</a>
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<li><a href="https://2014.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Measurement">Measurement Track</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Safety">Safety</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Interlab">Interlab Study</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/NEGEM">NEGEM</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/HumanPractices">Policy and Practices</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Acknowledgements">ACKNOWLEDGEMENTS</a>
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<td><center><a href="https://2015.igem.org/Team:BostonU/Temporal_Control"><img style="height:85%; width:85%;" src="https://static.igem.org/mediawiki/2015/thumb/9/99/Active_and_inactive_protein.png/800px-Active_and_inactive_protein.png" /></a><center></td>
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<td><center><a href="https://2015.igem.org/Team:BostonU/App_1/Motivation"><img style="height:85%; width:85%; padding-left:30px; padding-right:30px;" src="https://static.igem.org/mediawiki/2015/thumb/8/86/Home_page_integrase_RDF.png/562px-Home_page_integrase_RDF.png" /></a></center></td>
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<td><center><a href="https://2015.igem.org/Team:BostonU/App_2/Motivation"><img style="height:60%; width:60%;" src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Crispr_cas9_labeled.png/800px-Crispr_cas9_labeled.png" /></a></center></td>
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<td><h3 align="center" style="padding-bottom:50px;">Controlling Protein Activity</h3></td>
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<td><h3 align="center" style="padding-bottom:50px;">Controlling Integrases and RDFs</h3></td>
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<td><h3 align="center" style="padding-bottom:50px;">Controlling saCas9</h3></td>
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<td><center><a href="https://2015.igem.org/Team:BostonU/Mammalian_synbio/Significance"><img style="height:50%; width:50%;" src="https://static.igem.org/mediawiki/2015/thumb/5/59/Mammalian_syn_bio_home_page.png/618px-Mammalian_syn_bio_home_page.png" /></a></center></td>
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<td><center><a align="center" href="https://2015.igem.org/Team:BostonU/Education/Building_with_Biology"><img style="height:95%; width:95%; padding-left:30px; padding-right:30px;" src="https://static.igem.org/mediawiki/2015/c/c5/Building_w_biology.jpg" /></a></center></td>
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<td><center><a href="https://2015.igem.org/Team:BostonU/Attributions"><img style="height:200px; width:200px;" src="https://static.igem.org/mediawiki/2015/3/39/Thank_you_attributions.png"/></a></center></td>
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<td><h3 align="center">Mammalian Synthetic Biology</h3></td>
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<td><h3 align="center">Education and Outreach</h3></td>
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<a href="#Lower" rel="" id="#Lower" class="anchorLink"><img src="https://static.igem.org/mediawiki/2014/9/9e/Start_bu14.png" width="166" height="48" alt="Start"></a>
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<td><h3 align="center">Attributions</h3></td>
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<br><br><br><br><br><br><br><br>
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<h4 style="font-size:25px; text-align:center;">iGEM Jamboree 2015 Results</h4>
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<ol style="font-size:23px;text-align:center">Medal Received: Gold.<br>Nominated for Best Foundational Advance Project</li>
  
  
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<header1>Chimera</header1><h3>an optimized characterization workflow for synthetic biology</h3>
 
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<h3>Abstract </h3>
 
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<strong>The Joy of Cloning: Chimera, a Recipe for Integrating Computational Tools with Experimental Protocols </strong><br>
 
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If BU can clone it, so can you! With a pinch of wet lab work and a dash of computational tools, we have developed a new recipe called <a href="https://2014.igem.org/Team:BostonU/Workflow">Chimera</a> that will help fellow synthetic biology cloners in the creation of their genetic devices! Chimera utilizes <a href="https://2014.igem.org/Team:BostonU/Software">bio-design automation software tools</a> with experimental protocols and builds upon a thoroughly characterized library of <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo</a> parts. This recipe, or workflow, integrates software tools to reduce human error and to structure the way device designs are chosen, assembled, and tested. To demonstrate that this workflow can be used by any level of cloner (beginner, intermediate, and advanced), we will highlight how we used Chimera to create:<br>
 
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<p class="tab"><li> individual genetic parts (namely <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>, <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>, and <a href="https://2014.igem.org/Team:BostonU/Backbones">different backbones</a>), </p>
 
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<p class="tab"><li> transcriptional units assembled from individual parts (with both new and previously made MoClo parts), and </p>
 
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<p class="tab"><li> a complex genetic device (our goal is a <a href="https://2014.igem.org/Team:BostonU/Encoder">priority encoder</a>).</p>
 
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<br><br><strong><a href="https://2014.igem.org/Team:BostonU/Chimera">Click here to explore our project!</a></strong></td></tr></table>
 
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<div class="sponsors" align="center"> <br><br><header1>Our Sponsors</header1><br><br><img src="https://static.igem.org/mediawiki/2014/c/c5/Sponsors_bu14.png" width="983" height="149"> </div>
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Latest revision as of 20:30, 3 October 2015

Home

Developing conditionally dimerizable split protein systems for genetic logic and genome editing applications

The field of synthetic biology seeks to engineer desirable cellular functionalities by developing molecular technologies that enable precise genetic manipulation. A promising solution is to reliably control proteins that naturally execute genetic modifications. Current strategies to regulate activity of such proteins primarily rely on modulating protein expression level through transcriptional control; however, these methods are susceptible to slow response and leaky expression. In contrast, strategies that exploit post-translational regulation of activity, such as conditional dimerization of split protein halves, have been demonstrated to bypass these limitations. Here, we compare the relative efficiency of previously characterized dimerization domains in regulating activities of three important genetic manipulation proteins - integrases and recombination directionality factors for genetic logic applications, and saCas9 for in vivo genome editing applications. We also establish guidelines to rationally identify promising protein split sites. Our characterization of these systems in mammalian cells ultimately paves way for important biomedical applications.

Controlling Protein Activity

Controlling Integrases and RDFs

Controlling saCas9

Mammalian Synthetic Biology

Education and Outreach

Attributions








iGEM Jamboree 2015 Results

    Medal Received: Gold.
    Nominated for Best Foundational Advance Project