Difference between revisions of "Team:METU Turkey/Design"

 
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        <li>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey">Home</a>
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<td id="rcorners1" align=center > <a class="one" href="https://2015.igem.org/Team:METU_Turkey">HOME</a></td>
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<td id="rcorners1" align=center> <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Team">TEAM</a> </td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Project">PROJECT</a></td>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Team">Team</a>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Parts">PARTS</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Notebook">NOTEBOOK</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Attributions">ATTRIBUTIONS</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/interlab">INTERLAB</a></td>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Description">Project</a>
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            <ul>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Experiments">Experiments</a></li>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Results">Results</a></li>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Design">Design</a></li>
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            </ul>
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        </li>
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        <li>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Parts">Parts</a>
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            <ul>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Basic_Part">Basic Part</a></li>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Composite_Part">Composite Part</a></li>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Part_Collection">Part Collection</a></li>
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            </ul>
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            </li>
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        <li>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Notebook">Notebook</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Attributions">Attributions</a>
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        </li>
 +
        <li>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/interlab">Interlab</a>
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        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Collaborations">Collaborations</a>
 +
        </li>
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        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Practices">Human Practices</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Safety">Safety</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Gallery">Gallery</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Sponsors">Sponsors</a>
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        </li>
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 +
        </li>
 +
    </ul>
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<!-- First block of content -->
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<table  cellspacing="20px" height="150px" align="center">
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<td align="center" width="350px"><a href="https://igem.org/Main_Page" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="150px"></a></td>
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<img src="https://static.igem.org/mediawiki/2014/c/cc/Metuigem_logo.png" width="120px">
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<h3>Welcome!  <br> We are Team METU_Turkey! </h3>
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</td>
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<td align="center" width="162px"><a href="http://www.metu.edu.tr"color:#FFFFFF" target="_blank"><img src="https://static.igem.org/mediawiki/2014/9/91/Metulogo.jpg" width="300px"></a></td>
 
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Collaborations">COLLABORATIONS</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Practices">HUMAN PRACTICES</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Safety">SAFETY</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Modeling">MODELING</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Measurement">MEASUREMENT</a></td>
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<h4 align=center>Project Circuit</h4>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Galery">GALERY</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Sponsors">SPONSORS</a></td>
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/6/6a/K1606007.PNG" width="600px">
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/f/fb/K1606010.PNG" width="600px">
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/f/f9/K1606029.PNG" width="600px">
 
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<p align=center>We modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation.
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<img align=center src="https://static.igem.org/mediawiki/2015/f/f9/LO_ODTU_METU_ACIK_RENKLI_ZEMIN.png" width=100%></br>
 
  
  
 
<table>
 
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<h4 align=center>Kill Switch Circuit</h4>
 
<tr>
 
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/0/00/Celiac_stockphoto_1.jpg" width="600px">
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<div class="highlightBox">
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<h2 align=center>Project Description</h2>
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<p align=center>Strong promoter without thickener of LuxR and antiholin sequence:
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<p align=center>As an autoimmune disorder, celiac disorder depends on sensitivity to wheat ingredients such that gliadin which is a glycoprotein complex rich in proline and glutamine, especially alpha-2-gliadin. Alfa-2-glaidin basically bind to of specific receptor called HLA-DQ2 or HLA-DQ8 (because tTG alter the alfa-2-gliadin’s charge density which results in highly negative charges. This negative charge density create significant binding effect of alfa-2-gliadin to HLA-DQ2 OR HLA-DQ8 ). That receptor is an important key component of immune system, which have loci at chromosome 6. Epitopes of that receptor create cellular signaling pathway which results in arise of T and B-cells. These important immune cells eventually kill the specific antigens presented cells. Thus, in order to interfere this autoimmune disorder, protein’s (gluten) specific subunits, which have key roles at celiac disorder are going to be destroyed before the intake of protein in our project. Fundamentally, adding specific enzymes’ genes to E.coli’s genome. Then, E.coli will be added into dough while it is in fermentation process. So during the process of fermentation, specific enzymes’ genes will be transcribed and break down the gluten. To sum up, with the specific enzyme, which is expressed by E.coli, alters and break down the specific protein subunit which is key component of celiac disorder. In addition, we would like to improve the stiffness of gluten-free bread by letting bacteria to secrete additives. For the efficiency of the gluten destruction process, the homogenization of dough will be also improved by the bacteria secretion of additives. Last but not least, receptor complex within E.coli is going to be designed and enable bacteria to express receptor which is like HLA-DQ; in order to detect whether there is a gluten or not.</p>
+
For our kill switch model first we design a circuit that secrete antiholin LuxR gene to supress the activity of holin and endolysin enzymes for working under normal conditions by digesting gluten into its small particles. After this composite part we design a new circuit that our composite gene part secrete a thickener(Xanthan Gum) that dough doesn't lose its stability and viscose structure type for consumers' choice.
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<hr>
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/5/56/K1606020.PNG" width="600px">
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<tr>
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<div class="highlightBox">
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<p align=center>Lux promoter activated by thickener(Xanthan Gum) gene part of LuxR and it start to lyse the cell with holin and endolysin activity.
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</p>
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<div class="highlightBox">
 
</div>
 
</div>
 
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        <tr>
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<a href="http://www.metu.edu.tr" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/9/91/Metulogo.jpg" width="350px" align="center"></a>
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        </td>
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<table  id="firstblock" width="60%"  cellspacing="10px" height="200px" align="center">
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<td align="center" width="162px"><a href="http://odtuteknokent.com.tr/" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/4/47/Odtuteknokent.jpg" width="175px"></a></td>
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<td align="center" width="162px"><a href="http://www.kardiosis.com.tr/" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/d/d4/Kardiosis.jpg" width="175px"></a></td>
 +
 +
<td align="center" width="162px"><a href="http://www.tr-idea.com/" target="_blank"> <img src="https://static.igem.org/mediawiki/2015/c/c6/METU_Turkey_Idea.PNG" width="350px"></a></td>
 +
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</tr>
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</table>
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<table align="center" cellspacing="10px">
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<tr>
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<td align="center"><a href="http://www.sentegen.com/" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/d/dd/Sentegenlogoder.PNG" width="175px"></a></td>
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<td align="center"><a href="http://www.mathworks.com/" target="_blank"> <img src="https://static.igem.org/mediawiki/2015/a/ab/METU_Turkey_mathworks.png" width="175px"></a></td>
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Latest revision as of 13:29, 4 October 2015

Welcome!
We are Team METU_Turkey!

Project Circuit

We modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation.

Kill Switch Circuit

Strong promoter without thickener of LuxR and antiholin sequence: For our kill switch model first we design a circuit that secrete antiholin LuxR gene to supress the activity of holin and endolysin enzymes for working under normal conditions by digesting gluten into its small particles. After this composite part we design a new circuit that our composite gene part secrete a thickener(Xanthan Gum) that dough doesn't lose its stability and viscose structure type for consumers' choice.

Lux promoter activated by thickener(Xanthan Gum) gene part of LuxR and it start to lyse the cell with holin and endolysin activity.