Difference between revisions of "Team:TCU Taiwan/Modeling"
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<tr><td align="center"><h1><span style="font-family:Arial Black;"><font size="6"><font-weight: 700;>Disinfection Efficiency</font></span></h1></td></tr> | <tr><td align="center"><h1><span style="font-family:Arial Black;"><font size="6"><font-weight: 700;>Disinfection Efficiency</font></span></h1></td></tr> | ||
<tr><td><h1><span style="font-family:Calibri;text-align:justify;"><font size="5"> | <tr><td><h1><span style="font-family:Calibri;text-align:justify;"><font size="5"> | ||
| − | We had tested the disinfection ability of our AMPs and proved our system is really worked.First, we prepared 1 ml LB medium of <I>S. aureus</I> concentration of OD 0.01 at 600 nm. Than we added 1 ml sample into tube and measured the OD at 600 nm once in two hours. We had analyzed the data and made it into chart. | + | We had tested the disinfection ability of our AMPs and proved our system is really worked.First, we prepared 1 ml LB medium of <I>S. aureus</I> concentration of OD 0.01 at 600 nm. Than we added 1 ml sample into tube and measured the OD at 600 nm once in two hours.The experiments were carried out for 24 hours, and the result were collected at 0, 2nd, 4th, 6th and 24th hours. We had analyzed the data and made it into chart. |
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Revision as of 09:02, 12 October 2015
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We had collected the data of disinfection experiments and in vitro wound healing assay. Than we analyzed it into charts to represent our experimental achievement. |
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We had tested the disinfection ability of our AMPs and proved our system is really worked.First, we prepared 1 ml LB medium of S. aureus concentration of OD 0.01 at 600 nm. Than we added 1 ml sample into tube and measured the OD at 600 nm once in two hours.The experiments were carried out for 24 hours, and the result were collected at 0, 2nd, 4th, 6th and 24th hours. We had analyzed the data and made it into chart. |
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Conclusions: As the chart we can see that both Signiferin and Epinecidin-1 have ability to kill S. aureus. But the effect is not obvious. We speculated the concentration is not enough to see the best efficiency. |
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After cell migration experiments, we use ImageJ to measure the distance of the migration then compared them to interpret our results. We had collected data and analyze it to made charts. |
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| fig.1 HaCat cell tests for Signiferin | fig.1-1 HaCat cell tests for Signiferin |
In this group, Fig-1 shows there are no significant result, except Mock. The result of mock didn't show our expected. However, Fig. 1-1 has proven of significant effectiveness. When HaCat in 50l of the signiferin rarefied LB medium, the result indirect prove it has ability to help wound healing. |
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| fig.2 HaCat cell tests for Epi-1 and Signiferin | fig.2-1 HaCat cell tests for Epi-1 and Signiferin |
In the second group, there are no significant result in Fig. 2. Furthermore, in the compare of mock and HaCat in 100l signiferin rarefied LB medium, the Fig. 2-1, shows significant effectiveness. It's means Epi-1 and signiferin have proven wound healing indirectly. |
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| fig.3 HaCat cell tests for Epi-1 | fig.3-1 HaCat cell tests for Epi-1 |
In the this group, there are no significant result in the Mock and three control group. Additionally, the HaCat in 50l of Epi-1 rarefied LB medium, the Fig. 3-1, shows significant effectiveness. In other words, 50l Epi-1 has proven can help wound healing indirectly. |
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| Contact us tcutaiwan@gmail.com No.701, Sec. 3, Zhongyang Rd. Hualien 97004, Taiwan |