Difference between revisions of "Team:Birkbeck/Safety"
Srcraig1987 (Talk | contribs) |
Srcraig1987 (Talk | contribs) |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 24: | Line 24: | ||
<h2>Safety</h2> | <h2>Safety</h2> | ||
<p>The concept of clean as you go was enforced in both Labs that experiments by Owligo team was conducted. A clean lab is a safe lab.</p> | <p>The concept of clean as you go was enforced in both Labs that experiments by Owligo team was conducted. A clean lab is a safe lab.</p> | ||
| − | <h3>I. Safe Project Design</h3> | + | <h3>I. Safe Project Design</h3> |
| − | <p><img width="150px" src="https://static.igem.org/mediawiki/2015/3/3e/The_Birkbeck_COSHH_Store_iGEM_2015_Team_Birkbeck.jpeg" align="left" hspace="20" vspace="30">This year, the Birkbeck iGEM team did not handle any organism above Risk Group1. 2 strains of <i>E. coli</i> were used (DH5α & 10β). All transformantions (and subsequent transformants) were handled in a Cat 2 laminar flow hood as a precaution. None of the biobricks formed were of risk. </p> | + | <p><img min-width="72px"; max-width="150px" src="https://static.igem.org/mediawiki/2015/3/3e/The_Birkbeck_COSHH_Store_iGEM_2015_Team_Birkbeck.jpeg" align="left" hspace="20" vspace="30">This year, the Birkbeck iGEM team did not handle any organism above Risk Group1. 2 strains of <i>E. coli</i> were used (DH5α & 10β). All transformantions (and subsequent transformants) were handled in a Cat 2 laminar flow hood as a precaution. None of the biobricks formed were of risk. </p> |
| + | <p>For conducting experiments, standard operating procedure (SOPs) were drafted. Each SOP had to be approved by supervisors/advisors. After approval, a Control of Substances Hazardous to Health (COSHH) assessment was carried out. Every member of the team had to fully understandand and signed by each student which enters the lab.</p> | ||
<br> | <br> | ||
<br> | <br> | ||
| Line 34: | Line 35: | ||
<h3><a href="#">Chemical Bacterial Transformation COSHH Form</a></h3> | <h3><a href="#">Chemical Bacterial Transformation COSHH Form</a></h3> | ||
<div> | <div> | ||
| − | + | <iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/2/29/COSHH_Bacterial_chemical_transformation_iGEM_2015_Team_Birkbeck.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | |
</div> | </div> | ||
<br><hr><br> | <br><hr><br> | ||
| + | </div> | ||
<div class="accordion"> | <div class="accordion"> | ||
| − | |||
<h3><a href="#">Growth Curve & Culture Maintenance COSHH Form</a></h3> | <h3><a href="#">Growth Curve & Culture Maintenance COSHH Form</a></h3> | ||
<div> | <div> | ||
| − | + | <iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/c/c2/COSHH_Bacterial_growth_maintenance_iGEM_2015_Team_Birkbeck.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | |
</div> | </div> | ||
<br><hr><br> | <br><hr><br> | ||
| Line 48: | Line 49: | ||
<h3><a href="#">DNA Purification COSHH Form</a></h3> | <h3><a href="#">DNA Purification COSHH Form</a></h3> | ||
<div> | <div> | ||
| − | + | <iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/6/61/COSHH_DNA_purification_iGEM_2015_Team_Birkbeck.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | |
</div> | </div> | ||
<br><hr><br> | <br><hr><br> | ||
| Line 55: | Line 56: | ||
<h3><a href="#">PCR & Molecular Biology COSHH Form</a></h3> | <h3><a href="#">PCR & Molecular Biology COSHH Form</a></h3> | ||
<div> | <div> | ||
| − | + | <iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/8/8f/COSHH_PCR_and_molecular_biology_iGEM_2015_Team_Birkbeck.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | |
</div> | </div> | ||
<br><hr><br> | <br><hr><br> | ||
</div> | </div> | ||
| − | + | ||
<br> | <br> | ||
<br> | <br> | ||
| Line 66: | Line 67: | ||
<br> | <br> | ||
| − | <h3>II. Avoiding Environmental Contamination</h3> | + | <h3>II. Avoiding Environmental Contamination</h3> |
| − | <p><img width="150px" src="https://static.igem.org/mediawiki/2015/e/eb/Birkbeck_Biohazard_iGEM_2015_Team_Birkbeck.jpeg" align="right" hspace="20" vspace="30">In Lab 307 (Birkbeck Main Campus, Mallet Street), liquid cultures in glassware were autoclaved & washed. Cultures in plastic disposable tubes were decanted into glass tubes and autoclaved. The plastic were autoclaved & disposed of. Pipette tips used for transferring live cultures were bleached (10% vol/vol) prior to autoclaving.</p> | + | <p><img min-width="75px"; max-width="150px" src= "https://static.igem.org/mediawiki/2015/e/eb/Birkbeck_Biohazard_iGEM_2015_Team_Birkbeck.jpeg" align="right" hspace="20" vspace="30">In Lab 307 (Birkbeck Main Campus, Mallet Street), liquid cultures in glassware were autoclaved & washed. Cultures in plastic disposable tubes were decanted into glass tubes and autoclaved. The plastic were autoclaved & disposed of. Pipette tips used for transferring live cultures were bleached (10% vol/vol) prior to autoclaving.</p> |
<p>In Lab G09, glassware was soaked in virkon (30-60 minutes) prior to cleaning. In the case of disposable culture tubes, virkon was added for 30-60 minutes. Plastic tubes were rinsed & autoclaved.</p> | <p>In Lab G09, glassware was soaked in virkon (30-60 minutes) prior to cleaning. In the case of disposable culture tubes, virkon was added for 30-60 minutes. Plastic tubes were rinsed & autoclaved.</p> | ||
<br> | <br> | ||
| − | <h3>III. Extended Measures to Ensure Safety</h3> | + | <h3>III. Extended Measures to Ensure Safety</h3> |
<p>In each of the labs, a safety induction was carried out along with the distribution of the health & safety documentation for each of the labs. Prior to access being granted, all students MUST attend each induction (insert the documents).</p> | <p>In each of the labs, a safety induction was carried out along with the distribution of the health & safety documentation for each of the labs. Prior to access being granted, all students MUST attend each induction (insert the documents).</p> | ||
| − | <iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/c/c0/Birkbeck_Biosafety_Sheet_iGEM_2015_Team_Birkbeck.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | + | <div class="accordion"> |
| + | <h3><a href="#">Birkbeck Health & Safety (School of Biological Sciences)</a></h3> | ||
| + | <div> | ||
| + | <iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/c/c0/Birkbeck_Biosafety_Sheet_iGEM_2015_Team_Birkbeck.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | ||
| + | </div> | ||
| + | <br><hr><br> | ||
| + | </div> | ||
| + | |||
| + | <div class="accordion"> | ||
| + | <h3><a href="#">Birkbeck Health & Safety (Lab 307 Specific)</a></h3> | ||
| + | <div> | ||
<iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/8/83/Birkbeck_Biosafety_Sheet_iGEM_2015_Team_Birkbeck1.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | <iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/8/83/Birkbeck_Biosafety_Sheet_iGEM_2015_Team_Birkbeck1.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | ||
| + | </div> | ||
| + | <br><hr><br> | ||
| + | </div> | ||
| + | |||
| + | <div class="accordion"> | ||
| + | <h3><a href="#">UCL - Darwin Building Health & Safety Details With important Contact Details/Links.</a></h3> | ||
| + | <div> | ||
<iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/e/e0/UCL_Darwin_Building_Safety_Contacts_%26_Web_links.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | <iframe marginwidth="0px" allowfullscreen src="https://static.igem.org/mediawiki/2015/e/e0/UCL_Darwin_Building_Safety_Contacts_%26_Web_links.pdf" embedded="true" style="width:800px; height:1150px;" frameborder="0px"></iframe> | ||
| + | </div> | ||
| + | <br><hr><br> | ||
| + | </div> | ||
| + | |||
| − | |||
<br><hr><br> | <br><hr><br> | ||
<br> | <br> | ||
| − | + | ||
<!-- | <!-- | ||
| Line 92: | Line 113: | ||
| − | + | Biosafty birkbeck sign; edit URL - https://2015.igem.org/File:Birkbeck_Biohazard_iGEM_2015_Team_Birkbeck.jpeg | |
| − | + | image source - https://static.igem.org/mediawiki/2015/e/eb/Birkbeck_Biohazard_iGEM_2015_Team_Birkbeck.jpeg | |
| − | + | COSHH Store; Edit URL - https://2015.igem.org/File:The_Birkbeck_COSHH_Store_iGEM_2015_Team_Birkbeck.jpeg | |
| − | + | Image Source - https://static.igem.org/mediawiki/2015/3/3e/The_Birkbeck_COSHH_Store_iGEM_2015_Team_Birkbeck.jpeg | |
| Line 128: | Line 149: | ||
</div> | </div> | ||
| − | </div> | + | </div>--> |
| + | <!--button for Research--> | ||
| + | <a href="https://2015.igem.org/Team:Birkbeck/Research"><img width="400px" height="auto" src="https://static.igem.org/mediawiki/2015/c/c7/Rachel-elliot.jpg" border="0"/></a> | ||
| + | <p>Back to main Research page</p> | ||
</html> | </html> | ||
Latest revision as of 16:37, 17 October 2015
Safety
The concept of clean as you go was enforced in both Labs that experiments by Owligo team was conducted. A clean lab is a safe lab.
I. Safe Project Design
This year, the Birkbeck iGEM team did not handle any organism above Risk Group1. 2 strains of E. coli were used (DH5α & 10β). All transformantions (and subsequent transformants) were handled in a Cat 2 laminar flow hood as a precaution. None of the biobricks formed were of risk.
For conducting experiments, standard operating procedure (SOPs) were drafted. Each SOP had to be approved by supervisors/advisors. After approval, a Control of Substances Hazardous to Health (COSHH) assessment was carried out. Every member of the team had to fully understandand and signed by each student which enters the lab.
II. Avoiding Environmental Contamination
In Lab 307 (Birkbeck Main Campus, Mallet Street), liquid cultures in glassware were autoclaved & washed. Cultures in plastic disposable tubes were decanted into glass tubes and autoclaved. The plastic were autoclaved & disposed of. Pipette tips used for transferring live cultures were bleached (10% vol/vol) prior to autoclaving.
In Lab G09, glassware was soaked in virkon (30-60 minutes) prior to cleaning. In the case of disposable culture tubes, virkon was added for 30-60 minutes. Plastic tubes were rinsed & autoclaved.
III. Extended Measures to Ensure Safety
In each of the labs, a safety induction was carried out along with the distribution of the health & safety documentation for each of the labs. Prior to access being granted, all students MUST attend each induction (insert the documents).
Back to main Research page