Difference between revisions of "Team:KU Leuven/Research"
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− | We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation | + | We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation. |
</p> | </p> | ||
<br/> | <br/> | ||
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<div class="subimgrow"> | <div class="subimgrow"> | ||
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<div class="subimg"> | <div class="subimg"> | ||
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea"> | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea"> | ||
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/5/5e/KU_Leuven_Wiki_Button_-_Idea2.png" width="100%" > |
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− | <img src="https://static.igem.org/mediawiki/2015/ | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods"> |
+ | <img src="https://static.igem.org/mediawiki/2015/1/1a/KU_Leuven_Wiki_Button_-_Methods2.png" width="100%"> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="whitespaceside"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="subtextrow"> | ||
+ | <div class="whitespaceside"></div> | ||
+ | <div class="subtext"> | ||
+ | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea"> | ||
+ | <h2>Idea</h2> | ||
+ | <p> A detailed description about the interaction between our two cells and the genetic circuit can be found here. | ||
+ | <br/> | ||
+ | </p> | ||
+ | </a> | ||
</div> | </div> | ||
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− | <div class=" | + | <div class="subtext"> |
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods"> |
+ | <h2>Methods</h2> | ||
+ | <p> Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters. | ||
+ | </p> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="whitespaceside"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="subimgreadmore"> | ||
+ | <div class="whitespaceside"></div> | ||
+ | <div class="subimgrm"> | ||
+ | <a href=" https://2015.igem.org/Team:KU_Leuven/Research/Idea"> | ||
+ | <div id="more"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more"> | ||
+ | </div> | ||
+ | </a> | ||
</div> | </div> | ||
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− | <div class=" | + | <div class="subimgrm"> |
− | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods"> | |
+ | <div id="more"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="whitespaceside"></div> | ||
+ | </div> | ||
</div> | </div> | ||
+ | <div class="whiterow"></div> | ||
− | + | <div class="subsectionwrapper"> | |
+ | <div class="subimgrow"> | ||
+ | <div class="whitespace1"></div> | ||
+ | <div class="subimg"> | ||
+ | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_Wiki_Button_-_Parts2.png" width="100%"> | ||
+ | </a> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="whitespace"></div> |
− | <div class=" | + | |
− | <a href="https://2015.igem.org/Team:KU_Leuven/Research/ | + | <div class="subimg"> |
− | < | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part"> |
− | + | <img src="https://static.igem.org/mediawiki/2015/0/08/KU_Leuven_Wiki_Button_-_Composite_parts2.png" width="100%"> | |
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<div class="whitespace"> | <div class="whitespace"> | ||
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+ | |||
+ | <div class="subimg"> | ||
+ | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Results"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Results2.png" width="100%"> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="whitespace1"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="subtextrow"> | ||
+ | <div class="whitespace1"></div> | ||
<div class="subtext"> | <div class="subtext"> | ||
− | <a href=""> | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part"> |
− | <h2>Parts</h2> | + | <h2>Basic Parts</h2> |
− | <p> | + | <p> Our new, self-designed basic parts necessary to control cell-cell interactions and <i>E. Coli</i> motility.</p> |
− | < | + | |
− | + | ||
− | </ | + | |
− | </p> | + | |
</a> | </a> | ||
</div> | </div> | ||
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<div class="subtext"> | <div class="subtext"> | ||
− | <a href=""> | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part"> |
− | <h2> | + | <h2>Composite Parts</h2> |
− | <p> | + | <p> Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators. |
− | + | ||
− | + | ||
− | + | ||
</p> | </p> | ||
</a> | </a> | ||
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− | <a href=""> | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Results"> |
<h2>Results</h2> | <h2>Results</h2> | ||
<p> The results of our experiments will appear here. | <p> The results of our experiments will appear here. | ||
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− | |||
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</p> | </p> | ||
</a> | </a> | ||
</div> | </div> | ||
+ | <div class="whitespace1"></div> | ||
</div> | </div> | ||
<div class="subimgreadmore"> | <div class="subimgreadmore"> | ||
+ | <div class="whitespace1"></div> | ||
<div class="subimgrm"> | <div class="subimgrm"> | ||
− | <a href=" https://2015.igem.org/Team:KU_Leuven/Research/ | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part"> |
− | <div | + | <div id="more"> |
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more"> | <img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more"> | ||
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− | <a href=""> | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part"> |
− | <div | + | <div id="more"> |
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more"> | <img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more"> | ||
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<div class="subimgrm"> | <div class="subimgrm"> | ||
− | <a href=""> | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Results"> |
− | <div | + | <div id="more"> |
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more"> | <img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more"> | ||
</div> | </div> | ||
</a> | </a> | ||
+ | </div> | ||
+ | <div class="whitespace1"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="subimgrowm"> | ||
+ | <div class="subimgm"> | ||
+ | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea" > | ||
+ | <b>Idea</b> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/5/5e/KU_Leuven_Wiki_Button_-_Idea2.png" width="100%" ></a> | ||
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− | <div class=" | + | <div class="subtextm"> |
− | <a href | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea" > |
− | + | <p> | |
− | + | A detailed description about the interaction between our two cells and the genetic circuit can be found here. | |
+ | </p> | ||
+ | </a> | ||
+ | </div> | ||
</div> | </div> | ||
− | < | + | |
+ | <div class="subimgrowm"> | ||
+ | <div class="whiterow"> | ||
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<div class="subimgrowm"> | <div class="subimgrowm"> | ||
<div class="subimgm"> | <div class="subimgm"> | ||
− | <a href="https://2015.igem.org/Team:KU_Leuven/Research/ | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods" > |
− | <b> | + | <b>Methods</b> |
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/1/1a/KU_Leuven_Wiki_Button_-_Methods2.png" width="100%" ></a> |
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− | <a href="https://2015.igem.org/Team:KU_Leuven/Research/ | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods" > |
<p> | <p> | ||
− | + | Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters. | |
</p> | </p> | ||
</a> | </a> | ||
</div> | </div> | ||
− | |||
</div> | </div> | ||
+ | <div class="subimgrowm"> | ||
+ | <div class="whiterow"> | ||
+ | </div> | ||
+ | </div> | ||
<div class="subimgrowm"> | <div class="subimgrowm"> | ||
<div class="subimgm"> | <div class="subimgm"> | ||
− | <b>Parts</b> | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part" > |
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <b>Basic Parts</b> |
+ | <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_Wiki_Button_-_Parts2.png" width="100%" ></a> | ||
</div> | </div> | ||
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− | <div class="subtextm"> | + | <div class="subtextm"> |
− | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part" > | |
<p> | <p> | ||
− | + | Our new, self-designed basic parts necessary to control cell-cell interactions and <i>E. Coli</i> motility. | |
</p> | </p> | ||
</a> | </a> | ||
− | </div> | + | </div> |
</div> | </div> | ||
+ | <div class="subimgrowm"> | ||
+ | <div class="whiterow"> | ||
+ | </div> | ||
+ | </div> | ||
<div class="subimgrowm"> | <div class="subimgrowm"> | ||
<div class="subimgm"> | <div class="subimgm"> | ||
− | <b> | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part" > |
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <b>Composite Parts</b> |
+ | <img src="https://static.igem.org/mediawiki/2015/0/08/KU_Leuven_Wiki_Button_-_Composite_parts2.png" width="100%" ></a> | ||
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− | <div class="subtextm"> | + | <div class="subtextm"> |
− | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part" > | |
<p> | <p> | ||
− | + | Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators. | |
</p> | </p> | ||
</a> | </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="subimgrowm"> | ||
+ | <div class="whiterow"> | ||
</div> | </div> | ||
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<div class="subimgrowm"> | <div class="subimgrowm"> | ||
<div class="subimgm"> | <div class="subimgm"> | ||
+ | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Results" > | ||
<b>Results</b> | <b>Results</b> | ||
− | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Results2.png" width="100%" ></a> |
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− | + | <a href="https://2015.igem.org/Team:KU_Leuven/Research/Results" > | |
<p> | <p> | ||
− | + | Click here to discover our results | |
</p> | </p> | ||
</a> | </a> | ||
</div> | </div> | ||
</div> | </div> | ||
− | + | </div> | |
</div> | </div> | ||
</div> | </div> | ||
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<p style="font-size:1.3em; text-align: center"> | <p style="font-size:1.3em; text-align: center"> | ||
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee<br> | Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee<br> | ||
− | Telephone | + | Telephone: +32(0)16 32 73 19<br> |
− | + | Email: igem@chem.kuleuven.be<br> | |
</p> | </p> | ||
</div> | </div> | ||
+ | </div> | ||
+ | |||
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+ | <div class="whiterow"> | ||
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<div id="eppendorf"> | <div id="eppendorf"> | ||
<a href="https://www.eppendorf.com/BE-en/"><img src="https://static.igem.org/mediawiki/2015/9/96/KU_Leuven_Logo_Eppendorf_transparant.png" alt="Eppendorf" width="95%"></a> | <a href="https://www.eppendorf.com/BE-en/"><img src="https://static.igem.org/mediawiki/2015/9/96/KU_Leuven_Logo_Eppendorf_transparant.png" alt="Eppendorf" width="95%"></a> | ||
+ | </div> | ||
+ | <div id="saillart"> | ||
+ | <a href="http://www.glasatelier-saillart.be/English/english.html"><img src="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_Sponsor_Saillard.png" alt="Glasatelier Saillart" width="95%"></a> | ||
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− | <a | + | <a><img src="https://static.igem.org/mediawiki/2015/1/15/KUL_Ko-Lo_Instruments_logo_transparant.png" alt="Ko-Lo Instruments" width="95%"></a> |
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<a href="https://www.bioke.com/"><img src="https://static.igem.org/mediawiki/2015/e/e1/KUL_Biok%C3%A9_logo_transparant.png" alt="Bioké" width="95%"></a> | <a href="https://www.bioke.com/"><img src="https://static.igem.org/mediawiki/2015/e/e1/KUL_Biok%C3%A9_logo_transparant.png" alt="Bioké" width="95%"></a> | ||
</div> | </div> | ||
+ | <div class="logonormal"> | ||
+ | <div id="regensys"> | ||
+ | <a href="http://regenesys.eu/"><img src="https://static.igem.org/mediawiki/2015/e/eb/KU_Leuven_Logo_Regenesys_Transparant.png" alt="Regenesys" width="95%"></a> | ||
+ | </div> | ||
+ | <div class="whiterow"></div> | ||
<div id="thermofisher"> | <div id="thermofisher"> | ||
<a href="https://www.fishersci.com/us/en/home.html"><img src="https://static.igem.org/mediawiki/2015/a/aa/KUL_Fischer_Scientific_logo_transparant.png" alt="Thermo Fisher Scientific" width="95%"></a> | <a href="https://www.fishersci.com/us/en/home.html"><img src="https://static.igem.org/mediawiki/2015/a/aa/KUL_Fischer_Scientific_logo_transparant.png" alt="Thermo Fisher Scientific" width="95%"></a> | ||
</div> | </div> | ||
+ | </div> | ||
+ | <div class="logonormal2"> | ||
<div id="vwr"> | <div id="vwr"> | ||
<a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+¤tLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a> | <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+¤tLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a> | ||
</div> | </div> | ||
+ | <div class = "whiterow"></div> | ||
+ | <div id="lgc"> | ||
+ | <a href="http://www.lgcgroup.com/our-science/genomics-solutions/#.Vfx9V9yLTIU"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_LOGO_LGC.png" alt="LGC Genomics" width="80%"> | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
<div id="footerimg"> | <div id="footerimg"> | ||
<img src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png" width="95%"> | <img src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png" width="95%"> | ||
</div> | </div> | ||
+ | <div class="logonormal2"> | ||
<div id="gimv"> | <div id="gimv"> | ||
<a href="http://www.gimv.com/en"><img src="https://static.igem.org/mediawiki/2015/a/ac/KU_Leuven_Logo_Gimv_Transparant.png" alt="Gimv" width="95%"></a> | <a href="http://www.gimv.com/en"><img src="https://static.igem.org/mediawiki/2015/a/ac/KU_Leuven_Logo_Gimv_Transparant.png" alt="Gimv" width="95%"></a> | ||
</div> | </div> | ||
+ | <div class = "whiterow"></div> | ||
+ | <div id="sopach"> | ||
+ | <a href="http://www.sopachem.com/"><img src="https://static.igem.org/mediawiki/2015/5/55/KU_Leuven_Sopachem.jpeg" alt="Sopachem" width="95%"></a> | ||
+ | </div> | ||
+ | </div> | ||
<div id="machery"> | <div id="machery"> | ||
<a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a> | <a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a> | ||
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<a href="https://www.sigmaaldrich.com/belgium-nederlands.html"><img src="https://static.igem.org/mediawiki/2015/4/4b/KUL_Sigma-Aldrich_logo_transparant.png" alt="Sigma-Aldrich" width="95%"></a> | <a href="https://www.sigmaaldrich.com/belgium-nederlands.html"><img src="https://static.igem.org/mediawiki/2015/4/4b/KUL_Sigma-Aldrich_logo_transparant.png" alt="Sigma-Aldrich" width="95%"></a> | ||
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Latest revision as of 09:33, 20 October 2015
We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation.
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be