Difference between revisions of "Team:KU Leuven/Research"

 
(12 intermediate revisions by 5 users not shown)
Line 103: Line 103:
 
<div class="summary">
 
<div class="summary">
 
<p>
 
<p>
−
We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation and tissue regeneration.  
+
We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation.  
 
</p>
 
</p>
 
<br/>
 
<br/>
Line 115: Line 115:
 
<div class="subsectionwrapper">
 
<div class="subsectionwrapper">
 
<div class="subimgrow">
 
<div class="subimgrow">
−
<div class="whitespace1"></div>
+
<div class="whitespaceside"></div>
 
<div class="subimg">
 
<div class="subimg">
 
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea">
 
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea">
Line 130: Line 130:
 
</a>
 
</a>
 
</div>
 
</div>
−
 
+
<div class="whitespaceside"></div>
−
<div class="whitespace">
+
−
</div>
+
−
 
+
−
<div class="subimg">
+
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Results">
+
−
<img src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Results2.png" width="100%">
+
−
</a>
+
−
</div>
+
−
<div class="whitespace1"></div>
+
 
</div>
 
</div>
  
 
<div class="subtextrow">
 
<div class="subtextrow">
−
<div class="whitespace"></div>
+
<div class="whitespaceside"></div>
 
<div class="subtext">
 
<div class="subtext">
 
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea">
 
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Idea">
Line 159: Line 150:
 
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods">
 
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods">
 
  <h2>Methods</h2>
 
  <h2>Methods</h2>
−
  <p> Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.
+
  <p> Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters.
−
</p>
+
−
</a>
+
−
</div>
+
−
 
+
−
<div class="whitespace">
+
−
</div>
+
−
 
+
−
<div class="subtext">
+
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Results">
+
−
<h2>Results</h2>
+
−
<p> The results of our experiments will appear here.
+
 
</p>
 
</p>
 
</a>
 
</a>
 
</div>
 
</div>
 +
<div class="whitespaceside"></div>
 
</div>
 
</div>
  
 
<div class="subimgreadmore">
 
<div class="subimgreadmore">
−
<div class="whitespace1"></div>
+
<div class="whitespaceside"></div>
 
<div class="subimgrm">
 
<div class="subimgrm">
 
<a href=" https://2015.igem.org/Team:KU_Leuven/Research/Idea">
 
<a href=" https://2015.igem.org/Team:KU_Leuven/Research/Idea">
Line 196: Line 177:
 
</a>
 
</a>
 
</div>
 
</div>
−
 
+
<div class="whitespaceside"></div>
−
<div class="whitespace">
+
−
</div>
+
−
 
+
−
<div class="subimgrm">
+
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Results">
+
−
<div id="more">
+
−
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
+
−
</div>
+
−
</a>
+
−
</div>
+
 
</div>
 
</div>
 
</div>
 
</div>
 +
 +
<div class="whiterow"></div>
  
 
<div class="subsectionwrapper">
 
<div class="subsectionwrapper">
Line 214: Line 187:
 
<div class="whitespace1"></div>
 
<div class="whitespace1"></div>
 
<div class="subimg">
 
<div class="subimg">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part">
 
  <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_Wiki_Button_-_Parts2.png" width="100%">
 
  <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_Wiki_Button_-_Parts2.png" width="100%">
 
</a>
 
</a>
Line 222: Line 195:
  
 
<div class="subimg">
 
<div class="subimg">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part">
−
  <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_Wiki_Button_-_Parts2.png" width="100%">
+
  <img src="https://static.igem.org/mediawiki/2015/0/08/KU_Leuven_Wiki_Button_-_Composite_parts2.png" width="100%">
 
</a>
 
</a>
 
</div>
 
</div>
  
−
<div class="whitespace1"></div>
+
<div class="whitespace">
 +
</div>
  
 
<div class="subimg">
 
<div class="subimg">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Results">
−
  <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_Wiki_Button_-_Parts2.png" width="100%">
+
  <img src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Results2.png" width="100%">
 
</a>
 
</a>
 
</div>
 
</div>
Line 241: Line 215:
  
 
<div class="subtext">
 
<div class="subtext">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part">
−
  <h2>Parts</h2>
+
  <h2>Basic Parts</h2>
−
  <p> A list of all used biobricks, the modifications we performed on them and the new ones we designed.
+
  <p> Our new, self-designed basic parts necessary to control cell-cell interactions and <i>E. Coli</i> motility.</p>
−
</p>
+
 
</a>
 
</a>
 
</div>
 
</div>
Line 252: Line 225:
  
 
<div class="subtext">
 
<div class="subtext">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part">
−
  <h2>Parts</h2>
+
  <h2>Composite Parts</h2>
−
  <p> A list of all used biobricks, the modifications we performed on them and the new ones we designed.
+
  <p> Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.
 
</p>
 
</p>
 
</a>
 
</a>
 
</div>
 
</div>
 +
  
 
<div class="whitespace">
 
<div class="whitespace">
Line 263: Line 237:
  
 
<div class="subtext">
 
<div class="subtext">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Results">
−
  <h2>Parts</h2>
+
  <h2>Results</h2>
−
  <p> A list of all used biobricks, the modifications we performed on them and the new ones we designed.
+
  <p> The results of our experiments will appear here.
 
</p>
 
</p>
 
</a>
 
</a>
Line 275: Line 249:
 
<div class="whitespace1"></div>
 
<div class="whitespace1"></div>
 
<div class="subimgrm">
 
<div class="subimgrm">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part">
 
<div id="more">
 
<div id="more">
 
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
 
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
Line 286: Line 260:
  
 
<div class="subimgrm">
 
<div class="subimgrm">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part">
 
<div id="more">
 
<div id="more">
 
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
 
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
Line 294: Line 268:
  
 
<div class="whitespace">
 
<div class="whitespace">
−
</div>  
+
</div>
  
 
<div class="subimgrm">
 
<div class="subimgrm">
−
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts">
+
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Results">
 
<div id="more">
 
<div id="more">
 
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
 
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
Line 325: Line 299:
 
</div>
 
</div>
  
 +
<div class="subimgrowm">
 +
<div class="whiterow">
 +
</div>
 +
</div>
  
 +
<div class="subimgrowm">
 +
<div class="subimgm">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods" >
 +
    <b>Methods</b>
 +
<img src="https://static.igem.org/mediawiki/2015/1/1a/KU_Leuven_Wiki_Button_-_Methods2.png" width="100%" ></a>
 +
</div>
  
 
<div class="whitespace">
 
<div class="whitespace">
 +
</div>
 +
 +
  <div class="subtextm">
 +
      <a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods" >
 +
        <p>
 +
Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters.
 +
        </p>
 +
    </a>
 +
  </div>
 
</div>
 
</div>
  
Line 337: Line 330:
 
<div class="subimgrowm">
 
<div class="subimgrowm">
 
<div class="subimgm">
 
<div class="subimgm">
−
  <a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts" >
+
  <a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part" >
−
     <b>Parts</b>
+
     <b>Basic Parts</b>
 
  <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_Wiki_Button_-_Parts2.png" width="100%" ></a>
 
  <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_Wiki_Button_-_Parts2.png" width="100%" ></a>
 
</div>
 
</div>
Line 346: Line 339:
  
 
   <div class="subtextm">
 
   <div class="subtextm">
−
       <a href="https://2015.igem.org/Team:KU_Leuven/Research/Parts" >
+
       <a href="https://2015.igem.org/Team:KU_Leuven/Research/Basic_Part" >
 
         <p>
 
         <p>
−
           A detailed description about the interaction between our two cells and the genetic circuit can be found here.
+
           Our new, self-designed basic parts necessary to control cell-cell interactions and <i>E. Coli</i> motility.
 
         </p>
 
         </p>
 
     </a>  
 
     </a>  
Line 361: Line 354:
 
<div class="subimgrowm">
 
<div class="subimgrowm">
 
<div class="subimgm">
 
<div class="subimgm">
−
  <a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods" >
+
  <a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part" >
−
     <b>Methods</b>
+
     <b>Composite Parts</b>
−
  <img src="https://static.igem.org/mediawiki/2015/1/1a/KU_Leuven_Wiki_Button_-_Methods2.png" width="100%" ></a>
+
  <img src="https://static.igem.org/mediawiki/2015/0/08/KU_Leuven_Wiki_Button_-_Composite_parts2.png" width="100%" ></a>
 
</div>
 
</div>
  
Line 370: Line 363:
  
 
   <div class="subtextm">
 
   <div class="subtextm">
−
       <a href="https://2015.igem.org/Team:KU_Leuven/Research/Methods" >
+
       <a href="https://2015.igem.org/Team:KU_Leuven/Research/Composite_Part" >
 
         <p>
 
         <p>
−
Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.
+
          Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.
 
         </p>
 
         </p>
 
     </a>  
 
     </a>  
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
  
 
<div class="subimgrowm">
 
<div class="subimgrowm">
Line 459: Line 453:
 
   </div>
 
   </div>
 
  <div id="saillart">
 
  <div id="saillart">
−
       <a href="http://www.glasatelier-saillart.be/"><img src="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_Sponsor_Saillard.png" alt="Glasatelier Saillart" width="95%"></a>
+
       <a href="http://www.glasatelier-saillart.be/English/english.html"><img src="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_Sponsor_Saillard.png" alt="Glasatelier Saillart" width="95%"></a>
 
   </div>
 
   </div>
 
<div id="kuleuven">
 
<div id="kuleuven">
Line 487: Line 481:
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
<div class="logonormal2">
 
<div id="vwr">
 
<div id="vwr">
 
       <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+&currentLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a>
 
       <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+&currentLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a>
 
   </div>
 
   </div>
 +
<div class = "whiterow"></div>
 +
<div id="lgc">
 +
<a href="http://www.lgcgroup.com/our-science/genomics-solutions/#.Vfx9V9yLTIU">
 +
                <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_LOGO_LGC.png" alt="LGC Genomics" width="80%">
 +
</a>
 +
</div>
 +
</div>
 
  <div id="footerimg">
 
  <div id="footerimg">
 
   <img src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png" width="95%">
 
   <img src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png" width="95%">
 
   </div>
 
   </div>
 +
<div class="logonormal2">
 
  <div id="gimv">
 
  <div id="gimv">
 
       <a href="http://www.gimv.com/en"><img src="https://static.igem.org/mediawiki/2015/a/ac/KU_Leuven_Logo_Gimv_Transparant.png" alt="Gimv" width="95%"></a>
 
       <a href="http://www.gimv.com/en"><img src="https://static.igem.org/mediawiki/2015/a/ac/KU_Leuven_Logo_Gimv_Transparant.png" alt="Gimv" width="95%"></a>
 
   </div>
 
   </div>
 +
<div class = "whiterow"></div>
 +
<div id="sopach">
 +
      <a href="http://www.sopachem.com/"><img src="https://static.igem.org/mediawiki/2015/5/55/KU_Leuven_Sopachem.jpeg" alt="Sopachem" width="95%"></a>
 +
  </div>
 +
</div>
 
   <div id="machery">
 
   <div id="machery">
 
       <a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a>
 
       <a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a>

Latest revision as of 09:33, 20 October 2015

Logo

Logo

Research

Twitter
mail
mail

We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation.





Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Methods

Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters.

Read more
Read more

Basic Parts

Our new, self-designed basic parts necessary to control cell-cell interactions and E. Coli motility.

Composite Parts

Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.

Results

The results of our experiments will appear here.

Read more
Read more
Read more
Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Methods

Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters.

Basic Parts

Our new, self-designed basic parts necessary to control cell-cell interactions and E. Coli motility.

Composite Parts

Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.

Results

Click here to discover our results

Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be