Difference between revisions of "Team:KU Leuven/Research"

 
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We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation and tissue regeneration.  
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We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation.  
 
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  <p> Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.
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  <p> Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters.
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  <p> A list of all used biobricks, the modifications we performed on them and the new ones we designed.
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  <p> Our new, self-designed basic parts necessary to control cell-cell interactions and <i>E. Coli</i> motility.</p>
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  <p> A list of all used biobricks, the modifications we performed on them and the new ones we designed.
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  <p> Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.
 
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Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters.
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           A detailed description about the interaction between our two cells and the genetic circuit can be found here.
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           Our new, self-designed basic parts necessary to control cell-cell interactions and <i>E. Coli</i> motility.
 
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Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.
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          Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.
 
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       <a href="http://www.glasatelier-saillart.be/English/english.html"><img src="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_Sponsor_Saillard.png" alt="Glasatelier Saillart" width="95%"></a>
 
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       <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+&currentLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a>
 
       <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+&currentLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a>
 
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                <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_LOGO_LGC.png" alt="LGC Genomics" width="80%">
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       <a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a>
 
       <a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a>

Latest revision as of 09:33, 20 October 2015

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Research

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We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation.





Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Methods

Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters.

Read more
Read more

Basic Parts

Our new, self-designed basic parts necessary to control cell-cell interactions and E. Coli motility.

Composite Parts

Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.

Results

The results of our experiments will appear here.

Read more
Read more
Read more
Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Methods

Besides an overview of the performed steps to create two different cell types, you can also find a description of the detailed protein quantification methods to determine interesting parameters.

Basic Parts

Our new, self-designed basic parts necessary to control cell-cell interactions and E. Coli motility.

Composite Parts

Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.

Results

Click here to discover our results

Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be