Difference between revisions of "Team:KU Leuven/InterLabStudy"

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<head>
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<link rel="stylesheet" type="text/css"  
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<link rel="icon" href="https://static.igem.org/mediawiki/2015/9/9c/Ku_Leuven_Favicon.gif" />
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href="https://2015.igem.org/Template:KU_Leuven/Lightbox/CSS?action=raw&ctype=text/css" />
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<div id="topimg">
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    <h2> Interlab study </h2>
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<!------------------------------------------------------Begin Content--------------------------------------------------------->
 
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<div class="summarytext1">
 
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<div class="part">
 
  
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<h2>Results</h2>
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</div>
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<p>
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
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</p>
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<br>
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<p>
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<b>Table 1</b>
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−
Fluorescence measurements
+
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</p>
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</br>
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.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#fff;background-color:#f38630;}
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<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%">
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  <tr>
+
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    <th class="tg-031e">LB + Cam</th>
+
−
    <th class="tg-031e">LB+Cam+Cells</th>
+
−
    <th class="tg-s6z2" colspan="3">D1</th>
+
−
    <th class="tg-s6z2" colspan="3">D2</th>
+
−
    <th class="tg-s6z2" colspan="3">D3</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">512</td>
+
−
    <td class="tg-031e">601</td>
+
−
    <td class="tg-031e">737</td>
+
−
    <td class="tg-031e">685</td>
+
−
    <td class="tg-031e">751</td>
+
−
    <td class="tg-031e">9425</td>
+
−
    <td class="tg-031e">9322</td>
+
−
    <td class="tg-031e">9737</td>
+
−
    <td class="tg-031e">22786</td>
+
−
    <td class="tg-031e">25895</td>
+
−
    <td class="tg-031e">25048</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">546</td>
+
−
    <td class="tg-031e">594</td>
+
−
    <td class="tg-031e">754</td>
+
−
    <td class="tg-031e">641</td>
+
−
    <td class="tg-031e">641</td>
+
−
    <td class="tg-031e">9669</td>
+
−
    <td class="tg-031e">9545</td>
+
−
    <td class="tg-031e">9876</td>
+
−
    <td class="tg-031e">23159</td>
+
−
    <td class="tg-031e">26460</td>
+
−
    <td class="tg-031e">24785</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">549</td>
+
−
    <td class="tg-031e">587</td>
+
−
    <td class="tg-031e">722</td>
+
−
    <td class="tg-031e">660</td>
+
−
    <td class="tg-031e">697</td>
+
−
    <td class="tg-031e">9407</td>
+
−
    <td class="tg-031e">9321</td>
+
−
    <td class="tg-031e">9677</td>
+
−
    <td class="tg-031e">22719</td>
+
−
    <td class="tg-031e">25931</td>
+
−
    <td class="tg-031e">24935</td>
+
−
  </tr>
+
−
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
+
−
<br>
+
−
</table></div>
+
  
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<div id="summarywrapper">
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<div id="logowrapper">
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<div id="logoiGEMKUL">
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  <img src="https://static.igem.org/mediawiki/2015/2/23/Team_KU_Leuven_banner.jpg"  alt="Logo" width="100%" >
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</div>
 +
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<div id="center">
 +
<div id="pagetitle">
 +
<h2>Interlab Study</h2>
 +
</div>
 +
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<div id="scrolldown" class="center">
 +
<img src="https://static.igem.org/mediawiki/2015/1/1d/KU_Leuven_Arrows_wiki.png" alt="Scroll down" width="100%">
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<a href="https://twitter.com/kuleuven_igem" height="100%"><img src="https://static.igem.org/mediawiki/2015/b/b8/KU_Leuven_Twitter-logo.png" alt="Twitter" width="100%"></a> 
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<div id="summarylogofacebook">
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<a href="https://www.facebook.com/KULeuveniGEM?fref=ts" alt="Facebook" height="50px"><img src="https://static.igem.org/mediawiki/2015/d/df/KU_Leuven_Fblogo.png" alt="mail" width="100%"></a> 
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</div>
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<div id="summarylogomail">
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<a href="mailto:igem@chem.kuleuven.be"><img src="https://static.igem.org/mediawiki/2015/1/11/KU_Leuven_mail.png" alt="mail" width="100%"></a> 
 +
</div>
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</div>
 +
</div>
 +
<div class="summary">
 
<p>
 
<p>
−
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
+
This year the KU Leuven iGEM team decided to share its enthusiasm with the other teams to participate in the Interlab Measurement Study. The aim of this study was to construct specific devices combining a promoter with GFP and subsequently measure and analyse the fluorescence. By carrying out this study we want to contribute to the worldwide fluorescence database.
 +
 
 
</p>
 
</p>
 
<br>
 
<br>
−
<p>
 
−
<b>Table 2 </b> Absorbance measurements
 
−
</p>
 
−
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.tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#333;background-color:#fff;}
 
−
.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#fff;background-color:#f38630;}
 
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<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%" >
 
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−
    <th class="tg-031e">LB + Cam</th>
 
−
    <th class="tg-031e">LB+Cam+Cells</th>
 
−
    <th class="tg-s6z2" colspan="3">D1</th>
 
−
    <th class="tg-s6z2" colspan="3">D2</th>
 
−
    <th class="tg-s6z2" colspan="3">D3</th>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">0.0434</td>
 
−
    <td class="tg-031e">0.2052</td>
 
−
    <td class="tg-031e">0.2174</td>
 
−
    <td class="tg-031e">0.1942</td>
 
−
    <td class="tg-031e">0.2201</td>
 
−
    <td class="tg-031e">0.2088</td>
 
−
    <td class="tg-031e">0.2082</td>
 
−
    <td class="tg-031e">0.2154</td>
 
−
    <td class="tg-031e">0.2139</td>
 
−
    <td class="tg-031e">0.2210</td>
 
−
    <td class="tg-031e">0.2218</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">0.0405</td>
 
−
    <td class="tg-031e">0.2000</td>
 
−
    <td class="tg-031e">0.2182</td>
 
−
    <td class="tg-031e">0.1974</td>
 
−
    <td class="tg-031e">0.1980</td>
 
−
    <td class="tg-031e">0.2108</td>
 
−
    <td class="tg-031e">0.2111</td>
 
−
    <td class="tg-031e">0.2123</td>
 
−
    <td class="tg-031e">0.2143</td>
 
−
    <td class="tg-031e">0.2179</td>
 
−
    <td class="tg-031e">0.2056</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">0.0379</td>
 
−
    <td class="tg-031e">0.2064</td>
 
−
    <td class="tg-031e">0.2141</td>
 
−
    <td class="tg-031e">0.1961</td>
 
−
    <td class="tg-031e">0.2036</td>
 
−
    <td class="tg-031e">0.2137</td>
 
−
    <td class="tg-031e">0.2117</td>
 
−
    <td class="tg-031e">0.2164</td>
 
−
    <td class="tg-031e">0.2202</td>
 
−
    <td class="tg-031e">0.2261</td>
 
−
    <td class="tg-031e">0.2083</td>
 
−
  </tr>
 
−
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
 
 
<br>
 
<br>
−
</table>
 
−
<p>
 
−
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
 
−
</p>
 
 
<br>
 
<br>
−
 
−
<p>
 
−
<b>Table 3</b> Values for Biological replicates
 
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</p>
 
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.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#fff;background-color:#f38630;}
 
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<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%">
 
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−
    <th class="tg-031e"></th>
 
−
    <th class="tg-s6z2" colspan="3">D1</th>
 
−
    <th class="tg-s6z2" colspan="3">D2</th>
 
−
    <th class="tg-s6z2" colspan="3">D3</th>
 
−
    <th class="tg-s6z2">LB+Cam+Cells</th>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e"></td>
 
−
    <td class="tg-031e">T1</td>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e">T3</td>
 
−
    <td class="tg-031e">T1</td>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e">T3</td>
 
−
    <td class="tg-031e">T1</td>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e"></td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">B1</td>
 
−
    <td class="tg-031e">3390</td>
 
−
    <td class="tg-031e">3456</td>
 
−
    <td class="tg-031e">3372</td>
 
−
    <td class="tg-031e">45139</td>
 
−
    <td class="tg-031e">45868</td>
 
−
    <td class="tg-031e">44020</td>
 
−
    <td class="tg-031e">106526</td>
 
−
    <td class="tg-031e">108068</td>
 
−
    <td class="tg-031e">103174</td>
 
−
    <td class="tg-031e">2929</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">B2</td>
 
−
    <td class="tg-031e">3527</td>
 
−
    <td class="tg-031e">3247</td>
 
−
    <td class="tg-031e">3366</td>
 
−
    <td class="tg-031e">44774</td>
 
−
    <td class="tg-031e">45216</td>
 
−
    <td class="tg-031e">44029</td>
 
−
    <td class="tg-031e">117172</td>
 
−
    <td class="tg-031e">121432</td>
 
−
    <td class="tg-031e">114688</td>
 
−
    <td class="tg-031e">2970</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">B3</td>
 
−
    <td class="tg-031e">3399</td>
 
−
    <td class="tg-031e">3237</td>
 
−
    <td class="tg-031e">3426</td>
 
−
    <td class="tg-031e">45204</td>
 
−
    <td class="tg-031e">46519</td>
 
−
    <td class="tg-031e">44718</td>
 
−
    <td class="tg-031e">112931</td>
 
−
    <td class="tg-031e">120550</td>
 
−
    <td class="tg-031e">119707</td>
 
−
    <td class="tg-031e">2844</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">Brep Avg</td>
 
−
    <td class="tg-031e">3439</td>
 
−
    <td class="tg-031e">3313</td>
 
−
    <td class="tg-031e">3388</td>
 
−
    <td class="tg-031e">45039</td>
 
−
    <td class="tg-031e">45868</td>
 
−
    <td class="tg-031e">44256</td>
 
−
    <td class="tg-031e">112210</td>
 
−
    <td class="tg-031e">116683</td>
 
−
    <td class="tg-031e">112523</td>
 
−
    <td class="tg-031e" rowspan="2"></td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">BRep SD</td>
 
−
    <td class="tg-031e">270</td>
 
−
    <td class="tg-031e">101</td>
 
−
    <td class="tg-031e">162</td>
 
−
    <td class="tg-031e">189</td>
 
−
    <td class="tg-031e">532</td>
 
−
    <td class="tg-031e">327</td>
 
−
    <td class="tg-031e">4376</td>
 
−
    <td class="tg-031e">6102</td>
 
−
    <td class="tg-031e">6921</td>
 
−
  </tr>
 
−
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
 
−
<p>B1, B2, B3  <span id="tab2"> Biological replicates of the respective devices</span></p>
 
−
<p>T1, T2,  T3  <span id="tab3"> Technical replicates</span></p>
 
−
</br>
 
−
</table>
 
−
<p>
 
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−
</p>
 
 
<br>
 
<br>
−
<p>
+
</div>
−
<b> Table 4 </b> Values for Technical replicates
+
</div>
−
</p>
+
−
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+
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+
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+
−
.tg .tg-s6z2{text-align:center}
+
−
</style>
+
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<table class="tg" style="width:100%">
+
−
  <tr>
+
−
    <th class="tg-031e"></th>
+
−
    <th class="tg-s6z2" colspan="3">D1</th>
+
−
    <th class="tg-s6z2" colspan="3">D2</th>
+
−
    <th class="tg-s6z2" colspan="3">D3</th>
+
−
    <th class="tg-031e">LB+Cam+ Cells</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e"></td>
+
−
    <td class="tg-031e">B1</td>
+
−
    <td class="tg-031e">B2</td>
+
−
    <td class="tg-031e">B3</td>
+
−
    <td class="tg-031e">B1</td>
+
−
    <td class="tg-031e">B2</td>
+
−
    <td class="tg-031e">B3</td>
+
−
    <td class="tg-031e">B1</td>
+
−
    <td class="tg-031e">B2</td>
+
−
    <td class="tg-031e">B3</td>
+
−
    <td class="tg-031e"></td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">T1</td>
+
−
    <td class="tg-031e">3390</td>
+
−
    <td class="tg-031e">3527</td>
+
−
    <td class="tg-031e">3399</td>
+
−
    <td class="tg-031e">45139</td>
+
−
    <td class="tg-031e">44774</td>
+
−
    <td class="tg-031e">45204</td>
+
−
    <td class="tg-031e">106526</td>
+
−
    <td class="tg-031e">117172</td>
+
−
    <td class="tg-031e">112931</td>
+
−
    <td class="tg-031e">2929</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">T2</td>
+
−
    <td class="tg-031e">3456</td>
+
−
    <td class="tg-031e">3247</td>
+
−
    <td class="tg-031e">3237</td>
+
−
    <td class="tg-031e">45868</td>
+
−
    <td class="tg-031e">45216</td>
+
−
    <td class="tg-031e">46519</td>
+
−
    <td class="tg-031e">108068</td>
+
−
    <td class="tg-031e">121432</td>
+
−
    <td class="tg-031e">120550</td>
+
−
    <td class="tg-031e">2970</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">T3</td>
+
−
    <td class="tg-031e">3372</td>
+
−
    <td class="tg-031e">3366</td>
+
−
    <td class="tg-031e">3426</td>
+
−
    <td class="tg-031e">44020</td>
+
−
    <td class="tg-031e">44029</td>
+
−
    <td class="tg-031e">44718</td>
+
−
    <td class="tg-031e">103174</td>
+
−
    <td class="tg-031e">114688</td>
+
−
    <td class="tg-031e">119707</td>
+
−
    <td class="tg-031e">2844</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">TRep Avg</td>
+
−
    <td class="tg-031e">3406</td>
+
−
    <td class="tg-031e">3380</td>
+
−
    <td class="tg-031e">3354</td>
+
−
    <td class="tg-031e">45009</td>
+
−
    <td class="tg-031e">44673</td>
+
−
    <td class="tg-031e">45480</td>
+
−
    <td class="tg-031e">105923</td>
+
−
    <td class="tg-031e">117764</td>
+
−
    <td class="tg-031e">117729</td>
+
−
    <td class="tg-031e">2914</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">TRep SD</td>
+
−
    <td class="tg-031e">36</td>
+
−
    <td class="tg-031e">115</td>
+
−
    <td class="tg-031e">140</td>
+
−
    <td class="tg-031e">760</td>
+
−
    <td class="tg-031e">490</td>
+
−
    <td class="tg-031e">761</td>
+
−
    <td class="tg-031e">2043</td>
+
−
    <td class="tg-031e">2785</td>
+
−
    <td class="tg-031e">3410</td>
+
−
    <td class="tg-031e">52</td>
+
−
  </tr>
+
  
−
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
+
<div class="subsections">
−
<p>B1, B2, B3  <span id="tab2"> Biological replicates of the respective devices</span></p>
+
<div class="subsectionwrapper">
−
<p>T1, T2,  T3  <span id="tab3"> Technical replicates</span></p>
+
<div class="subimgrow">
−
</br>
+
<div class="whitespaceside"></div>
−
</table>
+
<div class="subimg">
−
<div>
+
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
−
<p>
+
<img src="https://static.igem.org/mediawiki/2015/1/1a/KU_Leuven_Wiki_Button_-_Methods2.png" width="100%" >
−
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+
</a>
−
</p>
+
</div>
−
<br>
+
−
<p>
+
−
<b> Table 5 </b> Standard graph
+
−
</p>
+
−
</br>
+
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+
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+
−
.tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#333;background-color:#fff;}
+
−
.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#fff;background-color:#f38630;}
+
−
.tg .tg-s6z2{text-align:center}
+
−
</style>
+
−
<table class="tg" style="width:100%">
+
−
  <tr>
+
−
    <th class="tg-s6z2" colspan="7">Fluorescein standard</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">concentration (ng/mL)</td>
+
−
    <td class="tg-031e">F1</td>
+
−
    <td class="tg-031e">F2</td>
+
−
    <td class="tg-031e">F3</td>
+
−
    <td class="tg-031e">average F</td>
+
−
    <td class="tg-031e">average F'</td>
+
−
    <td class="tg-031e">SD</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">0</td>
+
−
    <td class="tg-031e">9</td>
+
−
    <td class="tg-031e">13</td>
+
−
    <td class="tg-031e">12</td>
+
−
    <td class="tg-031e">11</td>
+
−
    <td class="tg-031e">0</td>
+
−
    <td class="tg-031e">1.699673171</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">10</td>
+
−
    <td class="tg-031e">1140</td>
+
−
    <td class="tg-031e">1168</td>
+
−
    <td class="tg-031e">1151</td>
+
−
    <td class="tg-031e">1153</td>
+
−
    <td class="tg-031e">1142</td>
+
−
    <td class="tg-031e">11.5181017</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">25</td>
+
−
    <td class="tg-031e">2543</td>
+
−
    <td class="tg-031e">2600</td>
+
−
    <td class="tg-031e">2631</td>
+
−
    <td class="tg-031e">2591</td>
+
−
    <td class="tg-031e">2580</td>
+
−
    <td class="tg-031e">36.4447832</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">50</td>
+
−
    <td class="tg-031e">4822</td>
+
−
    <td class="tg-031e">5097</td>
+
−
    <td class="tg-031e">5154</td>
+
−
    <td class="tg-031e">5024</td>
+
−
    <td class="tg-031e">5013</td>
+
−
    <td class="tg-031e">144.9513328</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">125</td>
+
−
    <td class="tg-031e">12879</td>
+
−
    <td class="tg-031e">12902</td>
+
−
    <td class="tg-031e">12931</td>
+
−
    <td class="tg-031e">12904</td>
+
−
    <td class="tg-031e">12893</td>
+
−
    <td class="tg-031e">21.27596453</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">250</td>
+
−
    <td class="tg-031e">25494</td>
+
−
    <td class="tg-031e">25761</td>
+
−
    <td class="tg-031e">25765</td>
+
−
    <td class="tg-031e">25673</td>
+
−
    <td class="tg-031e">25662</td>
+
−
    <td class="tg-031e">126.8183303</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">375</td>
+
−
    <td class="tg-031e">37474</td>
+
−
    <td class="tg-031e">37505</td>
+
−
    <td class="tg-031e">37899</td>
+
−
    <td class="tg-031e">37626</td>
+
−
    <td class="tg-031e">37615</td>
+
−
    <td class="tg-031e">193.4545597</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">500</td>
+
−
    <td class="tg-031e">46698</td>
+
−
    <td class="tg-031e">49051</td>
+
−
    <td class="tg-031e">48798</td>
+
−
    <td class="tg-031e">48182</td>
+
−
    <td class="tg-031e">48171</td>
+
−
    <td class="tg-031e">1054.652023</td>
+
−
  </tr>
+
  
−
</table>
+
<div class="whitespace">
−
<p>
+
</div>
−
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
+
−
</p>
+
−
<br>
+
−
<p>
+
−
<b> Table 6 </b> Extrapolation of GFP concentration using fluorescence measurements and fluorescein standard
+
−
</p>
+
−
</br>
+
−
<style type="text/css">
+
−
.tg  {border-collapse:collapse;border-spacing:0;border-color:#aaa; margin: 0px auto 100px auto; }
+
−
.tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#333;background-color:#fff;}
+
−
.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#aaa;color:#fff;background-color:#f38630;}
+
−
.tg .tg-s6z2{text-align:center}
+
−
</style>
+
−
<table class="tg" style="width:100%">
+
−
  <tr>
+
−
    <th class="tg-031e"></th>
+
−
    <th class="tg-s6z2" colspan="3">D1</th>
+
−
    <th class="tg-s6z2" colspan="3">D2</th>
+
−
    <th class="tg-s6z2" colspan="3">D3</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">Brep Avg</td>
+
−
    <td class="tg-031e">3439</td>
+
−
    <td class="tg-031e">3313</td>
+
−
    <td class="tg-031e">3388</td>
+
−
    <td class="tg-031e">45039</td>
+
−
    <td class="tg-031e">45868</td>
+
−
    <td class="tg-031e">44256</td>
+
−
    <td class="tg-031e">112210</td>
+
−
    <td class="tg-031e">116683</td>
+
−
    <td class="tg-031e">112523</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">(Brep Avg) - (LB+cam+cells avg)</td>
+
−
    <td class="tg-031e">525</td>
+
−
    <td class="tg-031e">399</td>
+
−
    <td class="tg-031e">474</td>
+
−
    <td class="tg-031e">42125</td>
+
−
    <td class="tg-031e">42954</td>
+
−
    <td class="tg-031e">41342</td>
+
−
    <td class="tg-031e">109296</td>
+
−
    <td class="tg-031e">113769</td>
+
−
    <td class="tg-031e">109609</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">Concentration (ng/mL)</td>
+
−
    <td class="tg-031e">1.929</td>
+
−
    <td class="tg-031e">0.639</td>
+
−
    <td class="tg-031e">1.407</td>
+
−
    <td class="tg-031e">427.88</td>
+
−
    <td class="tg-031e">436.36</td>
+
−
    <td class="tg-031e">419.88</td>
+
−
    <td class="tg-031e">1115.64</td>
+
−
    <td class="tg-031e">1161.44</td>
+
−
    <td class="tg-031e">1118.85</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">TRep Avg</td>
+
−
    <td class="tg-031e">3406</td>
+
−
    <td class="tg-031e">3380</td>
+
−
    <td class="tg-031e">3354</td>
+
−
    <td class="tg-031e">45009</td>
+
−
    <td class="tg-031e">44673</td>
+
−
    <td class="tg-031e">45480</td>
+
−
    <td class="tg-031e">105923</td>
+
−
    <td class="tg-031e">117764</td>
+
−
    <td class="tg-031e">117729</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">(Trep avg) - (LB+cam+cellsavg)</td>
+
−
    <td class="tg-031e">492</td>
+
−
    <td class="tg-031e">466</td>
+
−
    <td class="tg-031e">440</td>
+
−
    <td class="tg-031e">42095</td>
+
−
    <td class="tg-031e">41759</td>
+
−
    <td class="tg-031e">42566</td>
+
−
    <td class="tg-031e">103009</td>
+
−
    <td class="tg-031e">114850</td>
+
−
    <td class="tg-031e">114815</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">Concentration (ng/mL)</td>
+
−
    <td class="tg-031e">1.591</td>
+
−
    <td class="tg-031e">1.325</td>
+
−
    <td class="tg-031e">1.059</td>
+
−
    <td class="tg-031e">427.56</td>
+
−
    <td class="tg-031e">424.12</td>
+
−
    <td class="tg-031e">432.39</td>
+
−
    <td class="tg-031e">1051.27</td>
+
−
    <td class="tg-031e">1172.51</td>
+
−
    <td class="tg-031e">1172.15</td>
+
−
  </tr>
+
−
</table>
+
  
 +
<div class="subimg">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
 +
<img src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Results2.png" width="100%">
 +
</a>
 +
</div>
 +
<div class="whitespaceside"></div>
 +
</div>
  
 +
<div class="subtextrow">
 +
<div class="whitespaceside"></div>
 +
<div class="subtext">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
 +
<h2>Protocols</h2>
 +
<p>Detailed protocols and the worksheet can be found here.</p>
 +
</a>
 
</div>
 
</div>
 +
 +
<div class="whitespace">
 
</div>
 
</div>
  
 +
<div class="subtext">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
 +
<h2>Results</h2>
 +
<p>Click here to discover our results.</p>
 +
</a>
 +
</div>
 +
<div class="whitespaceside"></div>
 +
</div>
  
−
<div class="summarytext1">
+
<div class="subimgreadmore">
−
<div class="part">
+
<div class="whitespaceside"></div>
−
<h2>Protocols</h2>
+
<div class="subimgrm">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
 +
<div id="more">
 +
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
 +
</div>
 +
</a>
 +
</div>
  
−
<p>
+
<div class="whitespace">
 +
</div>  
  
−
<br>
+
<div class="subimgrm">
−
<br>
+
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
−
<br>
+
<div id="more">
−
<br>
+
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<b>Protocols</b>
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<img src="https://static.igem.org/mediawiki/2015/1/1a/KU_Leuven_Wiki_Button_-_Methods2.png" width="100%" >
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<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
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<p>Detailed protocols and the worksheet can be found here.</p>
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<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
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<p>Click here to discover our results.<br/></p>
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Latest revision as of 09:35, 20 October 2015

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Interlab Study

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This year the KU Leuven iGEM team decided to share its enthusiasm with the other teams to participate in the Interlab Measurement Study. The aim of this study was to construct specific devices combining a promoter with GFP and subsequently measure and analyse the fluorescence. By carrying out this study we want to contribute to the worldwide fluorescence database.





Protocols

Detailed protocols and the worksheet can be found here.

Results

Click here to discover our results.
Read more
Read more
Protocols

Detailed protocols and the worksheet can be found here.
Results

Click here to discover our results.

Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be