Difference between revisions of "Team:KU Leuven/InterLabStudy"

 
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#interlabstudy{
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<div id="logowrapper">
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<div id="center">
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<div class="summaryheader">
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<div id="pagetitle">
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  <div class="summaryimg">
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<h2>Interlab Study</h2>
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  <img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Researchbanner.jpg" width="100%">
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  <div class="head">
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    <h2> Interlab study </h2>
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<div id="scrolldown" class="center">
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<img src="https://static.igem.org/mediawiki/2015/1/1d/KU_Leuven_Arrows_wiki.png" alt="Scroll down" width="100%">
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</div>
  
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<!------------------------------------------------------Begin Content--------------------------------------------------------->
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</div>
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<div class="summarytext1">
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<div class="part">
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<h2>Results</h2>
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<div id="summarylogos">
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<p>
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
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</p>
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<br>
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<p>
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<b>Table 1</b>
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−
Fluorescence measurements
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</p>
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</br>
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<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%">
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−
  <tr>
+
−
    <th class="tg-031e">LB + Cam</th>
+
−
    <th class="tg-031e">LB+Cam+Cells</th>
+
−
    <th class="tg-s6z2" colspan="3">D1</th>
+
−
    <th class="tg-s6z2" colspan="3">D2</th>
+
−
    <th class="tg-s6z2" colspan="3">D3</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">512</td>
+
−
    <td class="tg-031e">601</td>
+
−
    <td class="tg-031e">737</td>
+
−
    <td class="tg-031e">685</td>
+
−
    <td class="tg-031e">751</td>
+
−
    <td class="tg-031e">9425</td>
+
−
    <td class="tg-031e">9322</td>
+
−
    <td class="tg-031e">9737</td>
+
−
    <td class="tg-031e">22786</td>
+
−
    <td class="tg-031e">25895</td>
+
−
    <td class="tg-031e">25048</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">546</td>
+
−
    <td class="tg-031e">594</td>
+
−
    <td class="tg-031e">754</td>
+
−
    <td class="tg-031e">641</td>
+
−
    <td class="tg-031e">641</td>
+
−
    <td class="tg-031e">9669</td>
+
−
    <td class="tg-031e">9545</td>
+
−
    <td class="tg-031e">9876</td>
+
−
    <td class="tg-031e">23159</td>
+
−
    <td class="tg-031e">26460</td>
+
−
    <td class="tg-031e">24785</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">549</td>
+
−
    <td class="tg-031e">587</td>
+
−
    <td class="tg-031e">722</td>
+
−
    <td class="tg-031e">660</td>
+
−
    <td class="tg-031e">697</td>
+
−
    <td class="tg-031e">9407</td>
+
−
    <td class="tg-031e">9321</td>
+
−
    <td class="tg-031e">9677</td>
+
−
    <td class="tg-031e">22719</td>
+
−
    <td class="tg-031e">25931</td>
+
−
    <td class="tg-031e">24935</td>
+
−
  </tr>
+
−
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
+
−
<br>
+
−
</table></div>
+
  
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<p>
+
<div id="summarylogotwitter">
−
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
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<a href="https://twitter.com/kuleuven_igem" height="100%"><img src="https://static.igem.org/mediawiki/2015/b/b8/KU_Leuven_Twitter-logo.png" alt="Twitter" width="100%"></a>
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</p>
+
</div>
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<br>
+
  
−
<p>
+
<div id="summarylogofacebook">
−
<b>Table 2 </b> Absorbance measurements
+
<a href="https://www.facebook.com/KULeuveniGEM?fref=ts" alt="Facebook" height="50px"><img src="https://static.igem.org/mediawiki/2015/d/df/KU_Leuven_Fblogo.png" alt="mail" width="100%"></a>   
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</p>
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</div>
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</br>
+
−
 
+
−
<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%" >
+
−
  <tr>
+
−
    <th class="tg-031e">LB + Cam</th>
+
−
    <th class="tg-031e">LB+Cam+Cells</th>
+
−
    <th class="tg-s6z2" colspan="3">D1</th>
+
−
    <th class="tg-s6z2" colspan="3">D2</th>
+
−
    <th class="tg-s6z2" colspan="3">D3</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">0.0434</td>
+
−
    <td class="tg-031e">0.2052</td>
+
−
    <td class="tg-031e">0.2174</td>
+
−
    <td class="tg-031e">0.1942</td>
+
−
    <td class="tg-031e">0.2201</td>
+
−
    <td class="tg-031e">0.2088</td>
+
−
    <td class="tg-031e">0.2082</td>
+
−
    <td class="tg-031e">0.2154</td>
+
−
    <td class="tg-031e">0.2139</td>
+
−
    <td class="tg-031e">0.2210</td>
+
−
    <td class="tg-031e">0.2218</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">0.0405</td>
+
−
    <td class="tg-031e">0.2000</td>
+
−
    <td class="tg-031e">0.2182</td>
+
−
    <td class="tg-031e">0.1974</td>
+
−
    <td class="tg-031e">0.1980</td>
+
−
    <td class="tg-031e">0.2108</td>
+
−
    <td class="tg-031e">0.2111</td>
+
−
    <td class="tg-031e">0.2123</td>
+
−
    <td class="tg-031e">0.2143</td>
+
−
    <td class="tg-031e">0.2179</td>
+
−
    <td class="tg-031e">0.2056</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">0.0379</td>
+
−
    <td class="tg-031e">0.2064</td>
+
−
    <td class="tg-031e">0.2141</td>
+
−
    <td class="tg-031e">0.1961</td>
+
−
    <td class="tg-031e">0.2036</td>
+
−
    <td class="tg-031e">0.2137</td>
+
−
    <td class="tg-031e">0.2117</td>
+
−
    <td class="tg-031e">0.2164</td>
+
−
    <td class="tg-031e">0.2202</td>
+
−
    <td class="tg-031e">0.2261</td>
+
−
    <td class="tg-031e">0.2083</td>
+
−
  </tr>
+
−
<p>D1, D2, D3 <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
+
−
<br>
+
−
</table>
+
  
 +
<div id="summarylogomail">
 +
<a href="mailto:igem@chem.kuleuven.be"><img src="https://static.igem.org/mediawiki/2015/1/11/KU_Leuven_mail.png" alt="mail" width="100%"></a> 
 +
</div>
 +
</div>
 +
</div>
 +
<div class="summary">
 
<p>
 
<p>
−
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
+
This year the KU Leuven iGEM team decided to share its enthusiasm with the other teams to participate in the Interlab Measurement Study. The aim of this study was to construct specific devices combining a promoter with GFP and subsequently measure and analyse the fluorescence. By carrying out this study we want to contribute to the worldwide fluorescence database.
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</p>
+
−
<br>
+
  
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<p>
 
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<b>Table 3</b> Values for Biological replicates
 
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</p>
 
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</br>
 
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<div class="tg-wrap" style="width:100%"><table class="tg" style="width:100%">
 
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  <tr>
 
−
    <th class="tg-031e"></th>
 
−
    <th class="tg-s6z2" colspan="3">D1</th>
 
−
    <th class="tg-s6z2" colspan="3">D2</th>
 
−
    <th class="tg-s6z2" colspan="3">D3</th>
 
−
    <th class="tg-s6z2">LB+Cam+Cells</th>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e"></td>
 
−
    <td class="tg-031e">T1</td>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e">T3</td>
 
−
    <td class="tg-031e">T1</td>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e">T3</td>
 
−
    <td class="tg-031e">T1</td>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e"></td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">B1</td>
 
−
    <td class="tg-031e">3390</td>
 
−
    <td class="tg-031e">3456</td>
 
−
    <td class="tg-031e">3372</td>
 
−
    <td class="tg-031e">45139</td>
 
−
    <td class="tg-031e">45868</td>
 
−
    <td class="tg-031e">44020</td>
 
−
    <td class="tg-031e">106526</td>
 
−
    <td class="tg-031e">108068</td>
 
−
    <td class="tg-031e">103174</td>
 
−
    <td class="tg-031e">2929</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">B2</td>
 
−
    <td class="tg-031e">3527</td>
 
−
    <td class="tg-031e">3247</td>
 
−
    <td class="tg-031e">3366</td>
 
−
    <td class="tg-031e">44774</td>
 
−
    <td class="tg-031e">45216</td>
 
−
    <td class="tg-031e">44029</td>
 
−
    <td class="tg-031e">117172</td>
 
−
    <td class="tg-031e">121432</td>
 
−
    <td class="tg-031e">114688</td>
 
−
    <td class="tg-031e">2970</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">B3</td>
 
−
    <td class="tg-031e">3399</td>
 
−
    <td class="tg-031e">3237</td>
 
−
    <td class="tg-031e">3426</td>
 
−
    <td class="tg-031e">45204</td>
 
−
    <td class="tg-031e">46519</td>
 
−
    <td class="tg-031e">44718</td>
 
−
    <td class="tg-031e">112931</td>
 
−
    <td class="tg-031e">120550</td>
 
−
    <td class="tg-031e">119707</td>
 
−
    <td class="tg-031e">2844</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">Brep Avg</td>
 
−
    <td class="tg-031e">3439</td>
 
−
    <td class="tg-031e">3313</td>
 
−
    <td class="tg-031e">3388</td>
 
−
    <td class="tg-031e">45039</td>
 
−
    <td class="tg-031e">45868</td>
 
−
    <td class="tg-031e">44256</td>
 
−
    <td class="tg-031e">112210</td>
 
−
    <td class="tg-031e">116683</td>
 
−
    <td class="tg-031e">112523</td>
 
−
    <td class="tg-031e" rowspan="2"></td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">BRep SD</td>
 
−
    <td class="tg-031e">270</td>
 
−
    <td class="tg-031e">101</td>
 
−
    <td class="tg-031e">162</td>
 
−
    <td class="tg-031e">189</td>
 
−
    <td class="tg-031e">532</td>
 
−
    <td class="tg-031e">327</td>
 
−
    <td class="tg-031e">4376</td>
 
−
    <td class="tg-031e">6102</td>
 
−
    <td class="tg-031e">6921</td>
 
−
  </tr>
 
−
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
 
−
<p>B1, B2, B3  <span id="tab2"> Biological replicates of the respective devices</span></p>
 
−
<p>T1, T2,  T3  <span id="tab3"> Technical replicates</span></p>
 
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</br>
 
−
</table>
 
−
<p>
 
−
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
 
 
</p>
 
</p>
 
<br>
 
<br>
−
<p>
 
−
<b> Table 4 </b> Values for Technical replicates
 
−
</p>
 
−
</br>
 
−
<table class="tg" style="width:100%">
 
−
  <tr>
 
−
    <th class="tg-031e"></th>
 
−
    <th class="tg-s6z2" colspan="3">D1</th>
 
−
    <th class="tg-s6z2" colspan="3">D2</th>
 
−
    <th class="tg-s6z2" colspan="3">D3</th>
 
−
    <th class="tg-031e">LB+Cam+ Cells</th>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e"></td>
 
−
    <td class="tg-031e">B1</td>
 
−
    <td class="tg-031e">B2</td>
 
−
    <td class="tg-031e">B3</td>
 
−
    <td class="tg-031e">B1</td>
 
−
    <td class="tg-031e">B2</td>
 
−
    <td class="tg-031e">B3</td>
 
−
    <td class="tg-031e">B1</td>
 
−
    <td class="tg-031e">B2</td>
 
−
    <td class="tg-031e">B3</td>
 
−
    <td class="tg-031e"></td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">T1</td>
 
−
    <td class="tg-031e">3390</td>
 
−
    <td class="tg-031e">3527</td>
 
−
    <td class="tg-031e">3399</td>
 
−
    <td class="tg-031e">45139</td>
 
−
    <td class="tg-031e">44774</td>
 
−
    <td class="tg-031e">45204</td>
 
−
    <td class="tg-031e">106526</td>
 
−
    <td class="tg-031e">117172</td>
 
−
    <td class="tg-031e">112931</td>
 
−
    <td class="tg-031e">2929</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">T2</td>
 
−
    <td class="tg-031e">3456</td>
 
−
    <td class="tg-031e">3247</td>
 
−
    <td class="tg-031e">3237</td>
 
−
    <td class="tg-031e">45868</td>
 
−
    <td class="tg-031e">45216</td>
 
−
    <td class="tg-031e">46519</td>
 
−
    <td class="tg-031e">108068</td>
 
−
    <td class="tg-031e">121432</td>
 
−
    <td class="tg-031e">120550</td>
 
−
    <td class="tg-031e">2970</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">T3</td>
 
−
    <td class="tg-031e">3372</td>
 
−
    <td class="tg-031e">3366</td>
 
−
    <td class="tg-031e">3426</td>
 
−
    <td class="tg-031e">44020</td>
 
−
    <td class="tg-031e">44029</td>
 
−
    <td class="tg-031e">44718</td>
 
−
    <td class="tg-031e">103174</td>
 
−
    <td class="tg-031e">114688</td>
 
−
    <td class="tg-031e">119707</td>
 
−
    <td class="tg-031e">2844</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">TRep Avg</td>
 
−
    <td class="tg-031e">3406</td>
 
−
    <td class="tg-031e">3380</td>
 
−
    <td class="tg-031e">3354</td>
 
−
    <td class="tg-031e">45009</td>
 
−
    <td class="tg-031e">44673</td>
 
−
    <td class="tg-031e">45480</td>
 
−
    <td class="tg-031e">105923</td>
 
−
    <td class="tg-031e">117764</td>
 
−
    <td class="tg-031e">117729</td>
 
−
    <td class="tg-031e">2914</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td class="tg-031e">TRep SD</td>
 
−
    <td class="tg-031e">36</td>
 
−
    <td class="tg-031e">115</td>
 
−
    <td class="tg-031e">140</td>
 
−
    <td class="tg-031e">760</td>
 
−
    <td class="tg-031e">490</td>
 
−
    <td class="tg-031e">761</td>
 
−
    <td class="tg-031e">2043</td>
 
−
    <td class="tg-031e">2785</td>
 
−
    <td class="tg-031e">3410</td>
 
−
    <td class="tg-031e">52</td>
 
−
  </tr>
 
−
 
−
<p>D1, D2, D3  <span id="tab1">Devices J23117 + I13504, J2310 + I13504 and J23101 + I13504 respectively</span></p>
 
−
<p>B1, B2, B3  <span id="tab2"> Biological replicates of the respective devices</span></p>
 
−
<p>T1, T2,  T3  <span id="tab3"> Technical replicates</span></p>
 
−
</br>
 
−
</table>
 
−
<div>
 
−
<p>
 
−
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
 
−
</p>
 
 
<br>
 
<br>
 +
<br>
 +
<br>
 +
</div>
 +
</div>
  
−
<p>
+
<div class="subsections">
−
<b> Table 5 </b> Standard graph
+
<div class="subsectionwrapper">
−
</p>
+
<div class="subimgrow">
 +
<div class="whitespaceside"></div>
 +
<div class="subimg">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
 +
<img src="https://static.igem.org/mediawiki/2015/1/1a/KU_Leuven_Wiki_Button_-_Methods2.png" width="100%" >
 +
</a>
 +
</div>
  
−
</br>
+
<div class="whitespace">
−
<table class="tg" style="width:100%">
+
</div>
−
  <tr>
+
−
    <th class="tg-s6z2" colspan="7">Fluorescein standard</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">concentration (ng/mL)</td>
+
−
    <td class="tg-031e">F1</td>
+
−
    <td class="tg-031e">F2</td>
+
−
    <td class="tg-031e">F3</td>
+
−
    <td class="tg-031e">average F</td>
+
−
    <td class="tg-031e">average F'</td>
+
−
    <td class="tg-031e">SD</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">0</td>
+
−
    <td class="tg-031e">9</td>
+
−
    <td class="tg-031e">13</td>
+
−
    <td class="tg-031e">12</td>
+
−
    <td class="tg-031e">11</td>
+
−
    <td class="tg-031e">0</td>
+
−
    <td class="tg-031e">1.699673171</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">10</td>
+
−
    <td class="tg-031e">1140</td>
+
−
    <td class="tg-031e">1168</td>
+
−
    <td class="tg-031e">1151</td>
+
−
    <td class="tg-031e">1153</td>
+
−
    <td class="tg-031e">1142</td>
+
−
    <td class="tg-031e">11.5181017</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">25</td>
+
−
    <td class="tg-031e">2543</td>
+
−
    <td class="tg-031e">2600</td>
+
−
    <td class="tg-031e">2631</td>
+
−
    <td class="tg-031e">2591</td>
+
−
    <td class="tg-031e">2580</td>
+
−
    <td class="tg-031e">36.4447832</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">50</td>
+
−
    <td class="tg-031e">4822</td>
+
−
    <td class="tg-031e">5097</td>
+
−
    <td class="tg-031e">5154</td>
+
−
    <td class="tg-031e">5024</td>
+
−
    <td class="tg-031e">5013</td>
+
−
    <td class="tg-031e">144.9513328</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">125</td>
+
−
    <td class="tg-031e">12879</td>
+
−
    <td class="tg-031e">12902</td>
+
−
    <td class="tg-031e">12931</td>
+
−
    <td class="tg-031e">12904</td>
+
−
    <td class="tg-031e">12893</td>
+
−
    <td class="tg-031e">21.27596453</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">250</td>
+
−
    <td class="tg-031e">25494</td>
+
−
    <td class="tg-031e">25761</td>
+
−
    <td class="tg-031e">25765</td>
+
−
    <td class="tg-031e">25673</td>
+
−
    <td class="tg-031e">25662</td>
+
−
    <td class="tg-031e">126.8183303</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">375</td>
+
−
    <td class="tg-031e">37474</td>
+
−
    <td class="tg-031e">37505</td>
+
−
    <td class="tg-031e">37899</td>
+
−
    <td class="tg-031e">37626</td>
+
−
    <td class="tg-031e">37615</td>
+
−
    <td class="tg-031e">193.4545597</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">500</td>
+
−
    <td class="tg-031e">46698</td>
+
−
    <td class="tg-031e">49051</td>
+
−
    <td class="tg-031e">48798</td>
+
−
    <td class="tg-031e">48182</td>
+
−
    <td class="tg-031e">48171</td>
+
−
    <td class="tg-031e">1054.652023</td>
+
−
  </tr>
+
  
−
</table>
+
<div class="subimg">
−
 
+
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
−
 
+
  <img src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Results2.png" width="100%">
−
<div class="center">
+
</a>
−
<div id="image1">
+
−
   
+
−
    <img src="https://static.igem.org/mediawiki/2015/c/c2/KU_Leuven_Standardcurve.png" style="width:100%">
+
−
<h4>
+
−
<div id=figure1> <b>Figure 1</b> </div>
+
−
Standard curve for fluorescein </h4>
+
 
</div>
 
</div>
 +
<div class="whitespaceside"></div>
 
</div>
 
</div>
  
−
<p>
+
<div class="subtextrow">
−
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
+
<div class="whitespaceside"></div>
−
</p>
+
<div class="subtext">
−
<br>
+
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
−
<p>
+
<h2>Protocols</h2>
−
<b> Table 6 </b> Extrapolation of GFP concentration using fluorescence measurements and fluorescein standard
+
<p>Detailed protocols and the worksheet can be found here.</p>
−
</p>
+
</a>
−
</br>
+
</div>
  
−
<table class="tg" style="width:100%">
+
<div class="whitespace">
−
  <tr>
+
</div>
−
    <th class="tg-031e"></th>
+
−
    <th class="tg-s6z2" colspan="3">D1</th>
+
−
    <th class="tg-s6z2" colspan="3">D2</th>
+
−
    <th class="tg-s6z2" colspan="3">D3</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">Brep Avg</td>
+
−
    <td class="tg-031e">3439</td>
+
−
    <td class="tg-031e">3313</td>
+
−
    <td class="tg-031e">3388</td>
+
−
    <td class="tg-031e">45039</td>
+
−
    <td class="tg-031e">45868</td>
+
−
    <td class="tg-031e">44256</td>
+
−
    <td class="tg-031e">112210</td>
+
−
    <td class="tg-031e">116683</td>
+
−
    <td class="tg-031e">112523</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">(Brep Avg) - (LB+cam+cells avg)</td>
+
−
    <td class="tg-031e">525</td>
+
−
    <td class="tg-031e">399</td>
+
−
    <td class="tg-031e">474</td>
+
−
    <td class="tg-031e">42125</td>
+
−
    <td class="tg-031e">42954</td>
+
−
    <td class="tg-031e">41342</td>
+
−
    <td class="tg-031e">109296</td>
+
−
    <td class="tg-031e">113769</td>
+
−
    <td class="tg-031e">109609</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">Concentration (ng/mL)</td>
+
−
    <td class="tg-031e">1.929</td>
+
−
    <td class="tg-031e">0.639</td>
+
−
    <td class="tg-031e">1.407</td>
+
−
    <td class="tg-031e">427.88</td>
+
−
    <td class="tg-031e">436.36</td>
+
−
    <td class="tg-031e">419.88</td>
+
−
    <td class="tg-031e">1115.64</td>
+
−
    <td class="tg-031e">1161.44</td>
+
−
    <td class="tg-031e">1118.85</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">TRep Avg</td>
+
−
    <td class="tg-031e">3406</td>
+
−
    <td class="tg-031e">3380</td>
+
−
    <td class="tg-031e">3354</td>
+
−
    <td class="tg-031e">45009</td>
+
−
    <td class="tg-031e">44673</td>
+
−
    <td class="tg-031e">45480</td>
+
−
    <td class="tg-031e">105923</td>
+
−
    <td class="tg-031e">117764</td>
+
−
    <td class="tg-031e">117729</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">(Trep avg) - (LB+cam+cellsavg)</td>
+
−
    <td class="tg-031e">492</td>
+
−
    <td class="tg-031e">466</td>
+
−
    <td class="tg-031e">440</td>
+
−
    <td class="tg-031e">42095</td>
+
−
    <td class="tg-031e">41759</td>
+
−
    <td class="tg-031e">42566</td>
+
−
    <td class="tg-031e">103009</td>
+
−
    <td class="tg-031e">114850</td>
+
−
    <td class="tg-031e">114815</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td class="tg-031e">Concentration (ng/mL)</td>
+
−
    <td class="tg-031e">1.591</td>
+
−
    <td class="tg-031e">1.325</td>
+
−
    <td class="tg-031e">1.059</td>
+
−
    <td class="tg-031e">427.56</td>
+
−
    <td class="tg-031e">424.12</td>
+
−
    <td class="tg-031e">432.39</td>
+
−
    <td class="tg-031e">1051.27</td>
+
−
    <td class="tg-031e">1172.51</td>
+
−
    <td class="tg-031e">1172.15</td>
+
−
  </tr>
+
−
</table>
+
  
 +
<div class="subtext">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
 +
<h2>Results</h2>
 +
<p>Click here to discover our results.</p>
 +
</a>
 +
</div>
 +
<div class="whitespaceside"></div>
 +
</div>
  
 +
<div class="subimgreadmore">
 +
<div class="whitespaceside"></div>
 +
<div class="subimgrm">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
 +
<div id="more">
 +
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
 
</div>
 
</div>
 +
</a>
 
</div>
 
</div>
  
 +
<div class="whitespace">
 +
</div>
  
−
<div class="summarytext1">
+
<div class="subimgrm">
−
<div class="part">
+
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
−
<h2>Protocols</h2>
+
<div id="more">
−
 
+
<img src="https://static.igem.org/mediawiki/2015/7/73/KUL_Wiki_Button_-_Read_more.png" height="40%" width="85%" alt="Read more">
−
<p>
+
−
 
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
</p>
+
 
</div>
 
</div>
 +
</a>
 
</div>
 
</div>
 +
<div class="whitespaceside"></div>
 
</div>
 
</div>
 +
 +
<div class="subimgrowm">
 +
<div class="subimgm">
 +
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
 +
<b>Protocols</b>
 +
<img src="https://static.igem.org/mediawiki/2015/1/1a/KU_Leuven_Wiki_Button_-_Methods2.png" width="100%" >
 +
</a>
 
</div>
 
</div>
  
 +
<div class="whitespace">
 +
</div>
  
−
<div class="panel panel-default">
+
<div class="subtextm">
−
        <div class="panel-heading panel-title" role="tab" id="heading1">
+
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Protocol">
−
          <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordionprotocols" href="#collapse1" aria-expanded="false" aria-controls="collapse1">
+
<p>Detailed protocols and the worksheet can be found here.</p>
−
Competent cells
+
</a>
−
          </a>
+
</div>
−
        </div>
+
</div>
−
        <div id="collapse1" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading1">
+
−
          <div class="panel-body">
+
−
            <ol style="list-style: decimal inside; font-size:19px;">
+
−
              <li>
+
−
                <p class="lead">Plate Top10 cells and incubate at 37ºC overnight</p>
+
−
              </li>
+
  
−
              <li>
+
<div class="subimgrowm">
−
                <p class="lead">Pick one colony, inoculate in LB media and incubate overnight while shaking at 37ºC</p>
+
<div class="whiterow">
−
              </li>
+
</div>
 +
</div>
  
−
              <li>
+
<div class="subimgrowm">
−
                <p class="lead">Dilute the culture in fresh medium, and continue the incubation until the OD<sub>600</sub>= 0.4-0.6</p>
+
<div class="subimgm">
−
              </li>
+
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
 +
<b>Results</b>
 +
<img src="https://static.igem.org/mediawiki/2015/e/e0/KU_Leuven_Wiki_Button_-_Results2.png" width="100%">
 +
</a>
 +
</div>
  
−
              <li>
+
<div class="whitespace">
−
                <p class="lead">Centrifugation 5 min. at 4000 rpm.</p>
+
</div>
−
              </li>
+
  
−
              <li>
+
<div class="subtextm">
−
                <p class="lead">Pellet cells and resuspend in 100mM of CaCl<sub>2</sub> solution</p>
+
<a href="https://2015.igem.org/Team:KU_Leuven/InterLabStudy/Results">
−
              </li>
+
<p>Click here to discover our results.<br/></p>
 +
</a>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
  
−
              <li>
+
<div id="summarywrapper">
−
                <p class="lead">Incubate on ice for 20 min</p>
+
<div class="summary">
−
              </li>
+
<h3> Contact </h3>
 +
<p style="font-size:1.3em; text-align: center">
 +
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee<br>
 +
Telephone: +32(0)16 32 73 19<br>
 +
Email: igem@chem.kuleuven.be<br> 
 +
</p>
 +
</div>
 +
</div>
  
−
              <li>
+
<div class="whiterow"></div>
−
                <p class="lead">Centrifugation 5 min at 3000 rpm</p>
+
−
              </li>
+
  
−
              <li>
+
<div id="footer">
−
                <p class="lead">Pellet cells and suspend again in 100mM CaCl<sub>2</sub> solution</p>
+
<div class="rowfooter">
−
              </li>
+
  <div class="emptyfooter">
 +
  </div>
 +
  <div id="bioscenter">
 +
      <a href="http://www.kuleuven.be/bioscenter/"><img src="https://static.igem.org/mediawiki/2015/3/3c/KU_Leuven_Logo_BioSCENTer.png" alt="bioSCENTer" width="95%"></a>
 +
  </div>
 +
  <div class="emptyfooter">
 +
  </div>
 +
</div>
  
−
              <li>
+
<div class="rowfooter">
−
                <p class="lead">Incubate on ice for 60 min</p>
+
      <div id="lrd">
−
              </li>
+
      <a href="http://lrd.kuleuven.be/"><img src="https://static.igem.org/mediawiki/2015/b/b4/KU_Leuven_HR_LRD_CMYK_LOGO.jpg" alt="KU Leuven Research & Development" width="95%"></a>
 +
  </div>
 +
  <div id="youreca">
 +
      <a href="http://www.kuleuven.be/onderzoek/youreca/"><img src="https://static.igem.org/mediawiki/2015/4/4f/KU_Leuven_YOURECA.png" alt="YOURECA" width="95%"></a>
 +
  </div>
 +
  <div id="solvay">
 +
      <a href="http://www.solvay.com/"><img src="https://static.igem.org/mediawiki/2015/8/89/KU_Leuven_Solvay_Transparant.png" alt="SOLVAY" width="95%"></a>
 +
  </div>
 +
  <div id="medicine">
 +
      <a href="http://gbiomed.kuleuven.be/english"><img src="https://static.igem.org/mediawiki/2015/8/83/KU_Leuven_Biomedische_Wetenschappen_logo_.png" alt="Faculty of Medicine" width="95%"></a>
 +
  </div>
 +
</div>
  
−
              <li>
+
  <div class="rowfooter">
−
                <p class="lead">Centrifugation 5 min at 3000 rpm</p>
+
<div id="imec">
−
              </li>
+
      <a href="http://www2.imec.be/be_en/home.html"><img src="https://static.igem.org/mediawiki/2015/7/70/KU_Leuven_Imec_logo_transparant.png" alt="imec" width="95%"></a>
 +
  </div>
 +
<div id="genzyme">
 +
      <a href="http://www.genzyme.be/"><img src="https://static.igem.org/mediawiki/2015/b/b7/KU_Leuven_Genzyme_logo_transparant.png" alt="Genzyme" width="95%"></a>
 +
  </div>
 +
  <div id="eppendorf">
 +
      <a href="https://www.eppendorf.com/BE-en/"><img src="https://static.igem.org/mediawiki/2015/9/96/KU_Leuven_Logo_Eppendorf_transparant.png" alt="Eppendorf" width="95%"></a>
 +
  </div>
 +
<div id="saillart">
 +
      <a href="http://www.glasatelier-saillart.be/English/english.html"><img src="https://static.igem.org/mediawiki/2015/c/ce/KU_Leuven_Sponsor_Saillard.png" alt="Glasatelier Saillart" width="95%"></a>
 +
  </div>
 +
<div id="kuleuven">
 +
      <a href="http://www.kuleuven.be/english"><img src="https://static.igem.org/mediawiki/2015/0/08/KULEUVEN_groot.png" alt="bioSCENTer" width="95%"></a>
 +
  </div>
 +
<div class="spacefooter">
 +
</div>
 +
<div id="gips">
 +
      <a href="http://www.gipsmineral.de/"><img src="https://static.igem.org/mediawiki/2015/3/3d/KU_Leuven_Gips_Mineral_logo_transparant.png" alt="Gips Mineral" width="95%"></a>
 +
  </div>
 +
<div id="kolo">
 +
      <a><img src="https://static.igem.org/mediawiki/2015/1/15/KUL_Ko-Lo_Instruments_logo_transparant.png" alt="Ko-Lo Instruments" width="95%"></a>
 +
    </div>
 +
</div>
  
−
              <li>
+
<div class="rowfooter">
−
                <p class="lead">Add a solution of 100mM CaCl<sub>2</sub> + 40% glycerol</p>
+
<div id="bioke">
−
              </li>
+
      <a href="https://www.bioke.com/"><img src="https://static.igem.org/mediawiki/2015/e/e1/KUL_Biok%C3%A9_logo_transparant.png" alt="Bioké" width="95%"></a>
 +
  </div>
 +
<div class="logonormal">
 +
<div id="regensys">
 +
      <a href="http://regenesys.eu/"><img src="https://static.igem.org/mediawiki/2015/e/eb/KU_Leuven_Logo_Regenesys_Transparant.png" alt="Regenesys" width="95%"></a>
 +
  </div>
 +
<div class="whiterow"></div>
 +
  <div id="thermofisher">
 +
      <a href="https://www.fishersci.com/us/en/home.html"><img src="https://static.igem.org/mediawiki/2015/a/aa/KUL_Fischer_Scientific_logo_transparant.png" alt="Thermo Fisher Scientific" width="95%"></a>
 +
  </div>
 +
</div>
 +
<div class="logonormal2">
 +
<div id="vwr">
 +
      <a href="https://be.vwr.com/store/?&_requestid=866148&_DARGS=/store/cms/be.vwr.com/nl_BE/header_20159241139103.jsp.1_AF&_dynSessConf=4047468000326453053&targetURL=/store/%3F%26_requestid%3D866148&lastLanguage=en&/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=&_D%3AcurrentLanguage=+&currentLanguage=en&_D%3AlastLanguage=+&_D%3A/vwr/userprofiling/EditPersonalInfoFormHandler.updateLocale=+"><img src="https://static.igem.org/mediawiki/2015/8/8d/KU_Leuven_Logo_VWR_transparant_.png" alt="VWR" width="95%"></a>
 +
  </div>
 +
<div class = "whiterow"></div>
 +
<div id="lgc">
 +
<a href="http://www.lgcgroup.com/our-science/genomics-solutions/#.Vfx9V9yLTIU">
 +
                <img src="https://static.igem.org/mediawiki/2015/e/e6/KU_Leuven_LOGO_LGC.png" alt="LGC Genomics" width="80%">
 +
</a>
 +
</div>
 +
</div>
 +
<div id="footerimg">
 +
  <img src="https://static.igem.org/mediawiki/2015/b/b9/KU_Leuven_Zebra_spots_wiki_footer_main.png" width="95%">
 +
  </div>
 +
<div class="logonormal2">
 +
<div id="gimv">
 +
      <a href="http://www.gimv.com/en"><img src="https://static.igem.org/mediawiki/2015/a/ac/KU_Leuven_Logo_Gimv_Transparant.png" alt="Gimv" width="95%"></a>
 +
  </div>
 +
<div class = "whiterow"></div>
 +
<div id="sopach">
 +
      <a href="http://www.sopachem.com/"><img src="https://static.igem.org/mediawiki/2015/5/55/KU_Leuven_Sopachem.jpeg" alt="Sopachem" width="95%"></a>
 +
  </div>
 +
</div>
 +
  <div id="machery">
 +
      <a href="http://www.filterservice.be/"><img src="https://static.igem.org/mediawiki/2015/4/41/KU_Leuven_Macherey_Nagel_logo_transparant.png" alt="Machery Nagel" width="95%"></a>
 +
  </div>
 +
<div class="logosmall">
 +
    <div id="sigma">
 +
      <a href="https://www.sigmaaldrich.com/belgium-nederlands.html"><img src="https://static.igem.org/mediawiki/2015/4/4b/KUL_Sigma-Aldrich_logo_transparant.png" alt="Sigma-Aldrich" width="95%"></a>
 +
    </div>
 +
    <div class="whiterow"></div>
 +
    <div id="egilab">
 +
      <a href="http://www.egilabo.be/"><img src="https://static.igem.org/mediawiki/2015/e/e9/KUL_Egilabo_logo_transparant.png" alt="Egilabo" width="95%"></a>
 +
    </div>
 +
    <div class="whiterow"></div>
 +
    <div id="novolab">
 +
      <a href="https://www.novolab.be/"><img src="https://static.igem.org/mediawiki/2015/4/4c/KU_Leuven_Novalab.png" alt="Novolab" height="95%"></a>
 +
    </div>
 +
  </div>  
 +
</div>
  
−
              <li>
 
−
                <p class="lead">Store immediately at -80ºC</p>
 
−
              </li>
 
−
            </ol>
 
−
          </div>
 
−
        </div>
 
−
      </div>
 
  
−
</br>
 
−
 
−
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Latest revision as of 09:35, 20 October 2015

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Interlab Study

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This year the KU Leuven iGEM team decided to share its enthusiasm with the other teams to participate in the Interlab Measurement Study. The aim of this study was to construct specific devices combining a promoter with GFP and subsequently measure and analyse the fluorescence. By carrying out this study we want to contribute to the worldwide fluorescence database.





Protocols

Detailed protocols and the worksheet can be found here.

Results

Click here to discover our results.
Read more
Read more
Protocols

Detailed protocols and the worksheet can be found here.
Results

Click here to discover our results.

Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be