Difference between revisions of "Team:Fudan"

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{{Fudan}}
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<html>
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<html>
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<head>
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<h2> Welcome to iGEM 2015! </h2>
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<meta charset="utf-8" />
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<meta http-equiv="x-ua-compatible" content="chrome=1">
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<meta http-equiv="x-ua-compatible" content="IE=10">
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<h4>Before you start: </h4>
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<script type="text/javascript" src="https://2015.igem.org/Team:Fudan/js/jquery?action=raw&ctype=text/javascript" defer="defer" charset="utf-8"></script>
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<p> Please read the following pages:</p>
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<script type="text/javascript" src="https://2015.igem.org/Team:Fudan/js/hovertreescroll?action=raw&ctype=text/javascript" defer="defer" charset="utf-8"></script>
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<ul>
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<script type="text/javascript" src="https://2015.igem.org/Team:Fudan/js/mousewheel?action=raw&ctype=text/javascript" defer="defer" language="JavaScript"></script>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<script type="text/javascript" src="https://2015.igem.org/Team:Fudan/js/crSpline?action=raw&ctype=text/javascript" defer="defer" charset="utf-8"></script>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<script type="text/javascript" src="https://2015.igem.org/Team:Fudan/js/index?action=raw&ctype=text/javascript" defer="defer" charset="utf-8"></script>
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</ul>
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<title>iGEM_Fudan</title>
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</head>
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<div class="highlightBox">
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<style type="text/css">
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<h4> Styling your wiki </h4>
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a{color:white!important;}a:visited {color:white!important;}*{color:#fff;font-family:"Source Sans Pro","Helvetica Neue",Helvetica,"Nimbus Sans L",Arial,"Liberation Sans","PingFang SC","Hiragino Sans GB","Source Han Sans CN","Source Han Sans SC","Microsoft YaHei","Wenquanyi Micro Hei","WenQuanYi Zen Hei","ST Heiti",SimHei,"WenQuanYi Zen Hei Sharp",sans-serif!important}body{overflow-y:hidden;color:#fff;font-family:"Source Sans Pro","Helvetica Neue",Helvetica,"Nimbus Sans L",Arial,"Liberation Sans","PingFang SC","Hiragino Sans GB","Source Han Sans CN","Source Han Sans SC","Microsoft YaHei","Wenquanyi Micro Hei","WenQuanYi Zen Hei","ST Heiti",SimHei,"WenQuanYi Zen Hei Sharp",sans-serif!important}#body{color:#fff;font-family:"Source Sans Pro","Helvetica Neue",Helvetica,"Nimbus Sans L",Arial,"Liberation Sans","PingFang SC","Hiragino Sans GB","Source Han Sans CN","Source Han Sans SC","Microsoft YaHei","Wenquanyi Micro Hei","WenQuanYi Zen Hei","ST Heiti",SimHei,"WenQuanYi Zen Hei Sharp",sans-serif!important;background-color:black;margin:0;display:block;min-width:1300px}.noprint{display:noe;}#content{width:100%;border:none;min-width:1300px;margin:0px;padding:0px}#globalWrapper{min-width:1300px;padding:0px;margin:0px;width:99.5vw;}#firstHeading{display:none}#main_index{position:relative;top:0;left:0;background:url("https://static.igem.org/mediawiki/2015/5/5b/Fudan_home1.jpg") no-repeat center center;background-size:cover;width:100%;height:100vh;min-height:667px}#scrolldown{position:absolute;left:76%;top:69%;width:78px;height:95px}#main_content{background:url("https://static.igem.org/mediawiki/2015/8/88/Fudan_home2.jpg") no-repeat center center;background-size:cover;display:inline-block;position:relative;top:0;width:100%;height:100vh;min-height:667px;padding-top: 10px}#left_row{float:left;width:30%}#right_row{float:right;margin-right:5%;margin-top:20px;}#right_row_title{font-size:40pt!important;text-align:center;margin-top:10vh;margin-bottom:4vh}#right_row_content{background-color:rgba(0,0,0,0.6);text-indent:2em;width:45vw;height:70vh;min-width:500px;min-height:400px;padding-left:10px;padding-top:10px}#right_row_content p{font-size:16px;margin-top:0;margin-bottom:0}#gear{position:absolute;z-index:10;margin-left:10.6%;margin-top:7.9%;width:247px}#logo{position:absolute;width:156px;margin-left:14.1%;margin-top:11.2%}.logoimg{width:260px}#achievement{position:absolute;left:390px;top:167px;width:146px}#design{position:absolute;left:370px;top:290px;width:91px}#model{position:absolute;left:299px;top:347px;width:91px}#parts{position:absolute;left:208px;top:367px;width:91px}#team{position:absolute;left:117px;top:334px;width:91px}#practice{position:absolute;left:52px;top:253px;width:91px}#my_info{font-size:16px;list-style-image:none;list-style-type:none;position:absolute;top:76%;margin-left:120px}#my_info li{padding-bottom:8px}#my_info a{color:white;text-decoration:none!important}
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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</style>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<body id="body">
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</div>
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<div id="loading" style="display:block; position: absolute; height:100%; width:100%; left: 0px; top: 0px; background-color: black; z-index: 20;">
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<img src="img/ring.gif" style="position:absolute; width:500px; margin: auto; top: 0; bottom: 0; left: 0; right: 0;"></img>
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<h4> Editing your wiki </h4>
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</div>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<div id="main_index">
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Fudan&action=edit"> Click here to edit this page! </a></p>
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<a id="scrolldown" href="javascript:void(0)" onclick="scroll('main_content')"></a>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
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</div>
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<div id="main_content">
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<div id="left_row">
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<h4>Templates </h4>
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<img id="gear" class="logoimg" src="https://static.igem.org/mediawiki/2015/6/60/Fudan_gear.png" />
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<img id="logo" class="logoimg" src="https://static.igem.org/mediawiki/2015/3/33/Fudan_logo.png" />
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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<a href="javascript:void(0)"><img id="achievement" class="logoimg" src="https://static.igem.org/mediawiki/2015/4/4b/Fudan_achievement.png" /></a>
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<a href="javascript:void(0)"><img id="design" class="logoimg" src="https://static.igem.org/mediawiki/2015/4/4a/Fudan_design.png" /></a>
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<a href="javascript:void(0)"><img id="model" class="logoimg" src="https://static.igem.org/mediawiki/2015/8/8a/Fudan_modeling.png" /></a>
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<h4>Tips</h4>
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<a href="javascript:void(0)"><img id="parts" class="logoimg" src="https://static.igem.org/mediawiki/2015/5/59/Fudan_parts.png" /></a>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<a href="javascript:void(0)"><img id="team" class="logoimg" src="https://static.igem.org/mediawiki/2015/f/f3/Fudan_team.png" /></a>
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<ul>
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<a href="javascript:void(0)"><img id="practice" class="logoimg" src="https://static.igem.org/mediawiki/2015/d/d1/Fudan_practice.png" /></a>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<ul id = "my_info">
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li><a href="http://songhao.xyz/iGEM_Fudan/collaboration.html">COLLABORATIONS</a></li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li><a href="http://songhao.xyz/iGEM_Fudan/notebook.html">NOTEBOOK</a></li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li><a href="mailto:bertalanffy@163.com">CONTACT US: bertalanffy@163.com</a></li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li><a href="javascript:scroll('main_index');">2015 iGEM HOME</a></li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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</ul>
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<li>Have lots of fun! </li>
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</div>
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</ul>  
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<div id="right_row">
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<div id="right_row_title">
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<span class = "achievement_title">ACHIEVEMENT</span>
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<h4>Inspiration</h4>
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<span class = "design_title" style="display: none;">DESIGN</span>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<span class = "modeling_title" style="display: none;">RESULTS</span>
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<ul>
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<span class = "parts_title" style="display: none;">PARTS</span>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<span class = "team_title" style="display: none;">ATTRIBUTION</span>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<span class = "practice_title" style="display: none;">PRACTICE</span>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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</div>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<div id="right_row_content">
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<div class = "achievement_title">
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<p>Circular RNAs (circRNAs), formed by non-sequential back-splicing of pre-mRNA transcripts, are a wide- spread form of non-coding RNA in animal cells. Various function of natural existed circRNA revealed recently shows that circRNA can be used as powerful tools in future research and health care. However, there is no toolbox to generate circRNA up to date, which restrict the research and application of circRNA. Our project focus on the devices to cyclize specific part of RNA, aiming to start a circRNA revolution.</p>
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</ul>
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<p>We designed three types of devices to cyclize the RNA based on the back-splicing mechanism:the "Ouroboros"(cyclizing device based on the inverted repeat sequence in the exon-flanking region), the "Cyclizer"(proteins that accelerate RNA cyclization)and the acRNA(ssRNA that accelerate RNA cyclization).</p>
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<p>We experimentally validate that our device can accelerate the formation of cirRNA. We also measured the half-life time of the circRNA, which proved its stability. To solidity our experiment procedure, we designed a device to report the level of mir-21 concentration and modeled the steady state of proteins to confirm our design.</p>
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<h4> Uploading pictures and files </h4>
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<p>Apart from our lab work, we also took active part in the educational outreach activities which has had huge influences on and off campus. As sociable participants in iGEM, we have collaboration with NYU_Shanghai, ZJU-China and NCTU Formosa, and mentored a high school team WLSA_Fudan-Shanghai. <a href="http://songhao.xyz/iGEM_Fudan/achievement.html">See more...</a></p>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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</div>
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<div class="design_title" style="display: none;">
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<p>Regulating oncomiRs is essential to fight cancer! However, the ordinary RNA sponge can be easily degraded. So we give the sponge a shield: we cyclize it! <a href="http://songhao.xyz/iGEM_Fudan/design.html">See more...</a></p>
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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<img src="https://static.igem.org/mediawiki/2015/a/a1/Design_for_homepage.jpg" style="width:80%;margin-left:10%;margin-top:10px;"></img>
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</div>
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<div class="parts_title" style="display: none;">
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<p>iGEM competition is based on standard parts, and we have modified most of our sequence to meet  the RFC. </p>
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</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
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<p>All of our parts to build the “Ouroboros” are all proved to be working as expected, as the testing experiment. Our mir-21 reporter also work as expected, which prove our design of mir-21 binding sites. </p>
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<p>We also improved several parts, we develop several linker based on former linker. Apart from that, we use beta-globin intron to provide SA and SD sequence in our circular RNA device, which is inspired by the beta-globin intron parts. <a href="http://songhao.xyz/iGEM_Fudan/parts.html">See more...</a></p>
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</div>
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<div class = "practice_title" style="display: none;">
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<p>We are working really hard to the outreach activities!</p>
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<p>One of the major work we focus on is the popularization of science. Inspired by one of the major incident in the debating of transgenic technology in China, our team hold six successful lecture in our university to share the basic knowledge of different areas of biology. We are not satisfied with our achievements on the campus, so we search places outside our university to make more difference. We work hard to get the chance to give a speech to all the students in a high school; we explain the simple principle of transgenic food to the passengers and hand out leaflets on the street; we introduced our project to visitors in Shanghai Science&Technology Museum. </p>
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<p>Besides all these activity, we also run the daily activity of Betalanffy Association, which is a famous community belonging to our iGEM team. <a href="http://songhao.xyz/iGEM_Fudan/practice.html">See more...</a></p>
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</div>
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<div class = "team_title" style="display: none;">
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<p>2015 Fudan is a fabulous team. We functioned specifically and managed every aspect of the iGEM competition within our team: project design, lab work, trouble-shooting, modeling, fundraising, human practice and wiki construction. With the generous help of our advisors and instructors, we brought here our final results. Also we offered our sincere thanks and acknowledgements for all the supporters involved in helping making a fabulous team. We do appreciate it. <a href="http://songhao.xyz/iGEM_Fudan/attribution.html">See more...</a></p>
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</div>
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<div class = "modeling_title" style="display: none;">
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<p>We have shown our design details of  three kinds of cyclizing device in  the DESIGN page:the Ouroboros（cyclizing device based on the inverted repeat sequence in the exon-flanking region), the Cyclizer（proteins that accelerate RNA cyclization)and the acRNA(ssRNA that accelerate RNA cyclization). </p>
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<p>All the three types of cyclizing devices we designed were actualized into sequence and parts, and we are currently working on testing and improve these devices. </p>
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<p>We experimentally validate that our device can accelerate the formation of cirRNA. </p>
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<p>We measured the half-life time of the circRNA produced by our device, which shows circRNA has significant higher stability.</p>
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<p>We designed a device to report the level of mir-21 concentration to support our experiment.</p>
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<p>We modeled the structure of proteins to confirm our “Cyclizer” design.</p>
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<p>We test cyclization based on acRNA and find circRNA never documented before! We are now working on new experiments to investigating it!</p>
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<p>Our devices provide huge possibility for cirRNA research and regulating oncomiRs, and we are still working to improve our device and develop our toolbox! <a href="http://songhao.xyz/iGEM_Fudan/results.html">See more...</a></p>
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</div>
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</div>
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</div>
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</div>
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</body>
 
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Latest revision as of 17:25, 20 October 2015

iGEM_Fudan

ACHIEVEMENT

Circular RNAs (circRNAs), formed by non-sequential back-splicing of pre-mRNA transcripts, are a wide- spread form of non-coding RNA in animal cells. Various function of natural existed circRNA revealed recently shows that circRNA can be used as powerful tools in future research and health care. However, there is no toolbox to generate circRNA up to date, which restrict the research and application of circRNA. Our project focus on the devices to cyclize specific part of RNA, aiming to start a circRNA revolution.

We designed three types of devices to cyclize the RNA based on the back-splicing mechanism:the "Ouroboros"(cyclizing device based on the inverted repeat sequence in the exon-flanking region), the "Cyclizer"(proteins that accelerate RNA cyclization)and the acRNA(ssRNA that accelerate RNA cyclization).

We experimentally validate that our device can accelerate the formation of cirRNA. We also measured the half-life time of the circRNA, which proved its stability. To solidity our experiment procedure, we designed a device to report the level of mir-21 concentration and modeled the steady state of proteins to confirm our design.

Apart from our lab work, we also took active part in the educational outreach activities which has had huge influences on and off campus. As sociable participants in iGEM, we have collaboration with NYU_Shanghai, ZJU-China and NCTU Formosa, and mentored a high school team WLSA_Fudan-Shanghai. See more...