Difference between revisions of "Team:NRP-UEA-Norwich/Parts"

 
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                                       <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle"  data-toggle="dropdown">Project</a>
 
                                       <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle"  data-toggle="dropdown">Project</a>
 
                                       <ul class="dropdown-menu">
 
                                       <ul class="dropdown-menu">
 +
                                        <li ><a href="/Team:NRP-UEA-Norwich/Description">Overview</a></li>
 
                                         <li ><a href="/Team:NRP-UEA-Norwich/Project">Background</a></li>
 
                                         <li ><a href="/Team:NRP-UEA-Norwich/Project">Background</a></li>
 
                                         <li ><a href="/Team:NRP-UEA-Norwich/Approach">Approach</a></li>
 
                                         <li ><a href="/Team:NRP-UEA-Norwich/Approach">Approach</a></li>
 
                                         <li ><a href="/Team:NRP-UEA-Norwich/Results">Results</a></li>
 
                                         <li ><a href="/Team:NRP-UEA-Norwich/Results">Results</a></li>
−
                                        <li ><a href="/Team:NRP-UEA-Norwich/Parts">Parts</a></li>
+
                                        <li ><a href="/Team:NRP-UEA-Norwich/Parts">Parts</a></li>
−
                                         <li ><a href="/Team:NRP-UEA-Norwich/Protocols">Protocols</a></li>
+
                                         <li ><a href="/Team:NRP-UEA-Norwich/Protocols">Protocols</a></li>                                   
−
                                        <li ><a href="/Team:NRP-UEA-Norwich/Notebook">Timeline</a></li>                                   
+
 
                                       </ul>
 
                                       </ul>
−
                                     <li class="dropdown " id="dropdown"><a href="/Team:NRP-UEA-Norwich/Modeling" class="dropdown-toggle">Modelling</a>
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                                     <li class="dropdown " id="dropdown"><a href="/Team:NRP-UEA-Norwich/ModelingAndSoftware" class="dropdown-toggle">Modelling</a>
 
                                       <ul class="dropdown-menu">
 
                                       <ul class="dropdown-menu">
−
                                         <li ><a href="/Team:NRP-UEA-Norwich/Modeling/Glyco2D">Glyco2D</a></li>
+
                                         <li ><a href="/Team:NRP-UEA-Norwich/Software">Glyco2D</a></li>
−
                                        <li ><a href="/Team:NRP-UEA-Norwich/Modeling/Simulation">Acylation Simulation</a></li>
+
 
                                         <li ><a href="/Team:NRP-UEA-Norwich/Modeling/3D">3D Models</a></li>
 
                                         <li ><a href="/Team:NRP-UEA-Norwich/Modeling/3D">3D Models</a></li>
−
                                         <li ><a href="/Team:NRP-UEA-Norwich/Modeling/Kinetic">Kinetic Model</a></li>                                  
+
                                         <li ><a href="/Team:NRP-UEA-Norwich/Modeling">Kinetic Model</a></li>                                
 +
 
 
                                       </ul>
 
                                       </ul>
 
                                   </li>     
 
                                   </li>     
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                                     <li  ><a href="/Team:NRP-UEA-Norwich/Attributions">Attributions</a></li>
 
                                     <li  ><a href="/Team:NRP-UEA-Norwich/Attributions">Attributions</a></li>
 
                                     <li  ><a href="/Team:NRP-UEA-Norwich/Practices">Practices</a></li>
 
                                     <li  ><a href="/Team:NRP-UEA-Norwich/Practices">Practices</a></li>
−
                                     <li  ><a href="/Team:NRP-UEA-Norwich/Collaborations">Collaborations</a></li>                                     
+
                                     <li  ><a href="/Team:NRP-UEA-Norwich/Collaborations">Collaborations</a></li>
 +
                                    <li  ><a href="/Team:NRP-UEA-Norwich/Achievements">Achievements</a></li>                                     
 
                                     </li>
 
                                     </li>
 
                        
 
                        
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     <h1 class="title1">Parts</h1>
 
     <h1 class="title1">Parts</h1>
 
     <div class="space30"></div>
 
     <div class="space30"></div>
 +
    <h2 class="title1">BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN</h2>
 +
    <div class="space10"></div>
  
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<table style="width:100%">
 
<table style="width:100%">
 
<tr>
 
<tr>
−
     <th>Part name</th>
+
     <th>Part Contents</th>
 
     <th>Backbone</th>  
 
     <th>Backbone</th>  
 
   <th>Description</th>
 
   <th>Description</th>
 
<th>Function</th>
 
<th>Function</th>
−
   <th>Part number</th>
+
   <th>Name in registry</th>
 
   </tr>
 
   </tr>
  
−
<tr><td>Plac-GlgX</td><td>pSB1C3</td><td>Debranching enzyme with IPTG inducible Promoter</td><td>Removes E. coli glycogen branches</td><td>BBa_K1618025</td></tr>
+
  <tr><td>GlgX</td><td>pSB1C3</td><td>Debranching Enzyme <i>(E. coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618025" style = "color: #002bb8;">BBa_K1618025</a></td></tr>
−
  <tr><td>GlgX</td><td>pSB1C3</td><td>Debranching enzyme</td><td>&nbsp;</td> <td>BBa_K1618026</td></tr>
+
−
<tr><td>Plac-GlgB</td><td>pSB1C3</td><td>Branching enzyme with IPTG inducible Promoter</td><td>Add E. coli glycogen branches</td>< td>BBa_K1618022</td></tr>
+
−
<tr><td>GlgB</td><td>pSB1C3</td><td>Branching Enzyme</td><td>&nbsp;</td> <td>BBa_K1618000</td></tr>
+
−
<tr><td>Plac-MAO</td><td>pSB1C3</td><td>Acylation candidate 1 with IPTG inducible Promoter</td><td>Acylates glycogen? (1)</td>< td>BBa_K1618005</td></tr>
+
−
<tr><td>MAO</td><td>pSB1C3</td><td>Acylation Candidate 1</td><td>&nbsp;</td> <td>BBa_K1618001</td></tr>
+
−
<tr><td>Plac-Rv3030</td><td>pSB1C3</td><td>Acylation candidate 2 with IPTG inducible Promoter</td><td>Acylates glycogen? (2)</td>< td>BBa_K1618007</td></tr>
+
−
<tr><td>Rv3030</td><td>pSB1C3</td><td>Acylation candidate 2</td><td>&nbsp;</td> <td>BBa_K1618002</td></tr>
+
−
<tr><td>Plac-Rv3034c</td><td>pSB1C3</td><td>Acylation candidate 3 with IPTG inducible Promoter</td><td>Acylates glycogen? (3)</td> <td>BBa_K1618008</td></tr>
+
−
<tr><td>Rv3034c</td><td>pSB1C3</td><td>Acylation candidate 3</td><td>&nbsp;</td> <td>BBa_K1618003</td></tr>
+
−
<tr><td>Plac-Rv3037c</td><td>pSB1C3</td><td>Acylation candidate 4 with IPTG inducible Promoter</td><td>Acylates glycogen? (4)</td> <td>BBa_K1618009</td></tr>
+
−
<tr><td>Rv3037c</td><td>pSB1C3</td><td>Acylation candidate 4</td><td>&nbsp;</td> <td>BBa_K1618004</td></tr>
+
−
<tr>< td>Plac-GlgB-Rv3030</ td>< td>pSB1C3</ td>< td>Branching enzyme + Acylation candidate 2 wi td IPTG inducible Promoter</td><td>Makes acetylated branched glycogen? (2)</ td>< td>Y</ td>< td>BBa_K1618023</td></tr>
+
−
<tr><td>Plac-GlgB-Rv3034c</td><td>pSB1C3</td><td>Branching enzyme + Acylation candidate 3 wi td IPTG inducible Promoter</td><td>Makes acetylated branched glycogen? (3)</td> td<td>BBa_K1618024</td></tr>
+
−
<tr><td>Plac-GlgB-Rv3037c</td><td>pSB1C3</td><td>Branching enzyme + Acylation candidate 4 wi td IPTG inducible Promoter</td><td>Makes acetylated branched glycogen? (4)</td> td<td>BBa_K1618027</td></tr>
+
−
<tr><td>Plac-Buk</td><td>pSB1C3</td><td>Butyrate kinase wi td IPTG inducible Promoter</td><td>Makes  butyryl CoA from butyrate part 1</td> td<td>BBa_K1618012</td></tr>
+
−
<tr><td>Plac-PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase wi td IPTG inducible Promoter</td><td>Makes  butyryl CoA from butyrate  part 2</td> td<td>BBa_K1618013</td></tr>
+
−
<tr><td>Plac- TDL</td><td>pSB1C3</td><td> tdiolase wi td IPTG inducible Promoter</td><td>Makes butyrate from acetate part 1</td> td<td>BBa_K1618014</td></tr>
+
−
<tr><td>Plac-CRO</td><td>pSB1C3</td><td>Crotonase wi td IPTG inducible Promoter</td><td>Makes butyrate from acetate part 2</td> td<td>BBa_K1618015</td></tr>
+
−
<tr><td>Plac-BHBD</td><td>pSB1C3</td><td>&#946;-hydroxybutyryl-CoA dehydrogenase wi td IPTG inducible Promoter</td><td>Makes butyrate from acetate part 3</td> td<td>BBa_K1618016</td></tr>
+
−
<tr><td>Plac-BCD</td><td>pSB1C3</td><td>Butyryl-CoA dehydrogenase wi td IPTG inducible Promoter</td><td>Makes butyrate from acetate part 4</td> td<td>BBa_K1618017</td></tr>
+
−
<tr><td>Plac-EFTb</td><td>pSB1C3</td><td>Electron transfer protein &#946; wi td IPTG inducible Promoter</td><td>Makes butyrate from acetate part 5</td> td<td>BBa_K1618018</td></tr>
+
−
<tr><td>Plac-EFTa</td><td>pSB1C3</td><td>Electron transfer protein &#945; wi td IPTG inducible Promoter</td><td>Makes butyrate from acetate part 6</td> td<td>BBa_K1618019</td></tr>
+
−
<tr><td>Plac-Buk-PTB</td><td>pSB1C3</td><td>Butyrate to butyryl CoA pa tdway wi td IPTG inducible Promoter</td><td>Makes  butyryl CoA from butyrate full</td> td<td>BBa_K1618020</td></tr>
+
  
 +
<tr><td>GlgB</td><td>pSB1C3</td><td>Branching Enzyme <i>(E. coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618000" style = "color: #002bb8;">BBa_K1618000</a></td></tr>
  
 +
<tr><td>MAO</td><td>pSB1C3</td><td>Acyltransferase Candidate 1 </td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618001" style = "color: #002bb8;">BBa_K1618001</a></td></tr>
 +
 +
<tr><td>Rv3030</td><td>pSB1C3</td><td>Acyltransferase Candidate 2</td><td>Coding sequence only</td><td><a href="http://parts.igem.org/Part:BBa_K1618002" style = "color: #002bb8;">BBa_K1618002</a></td></tr>
 +
 +
<tr><td>Rv3034c</td><td>pSB1C3</td><td>Acyltransferase Candidate 3</td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618003" style = "color: #002bb8;">BBa_K1618003</a></td></tr>
 +
 +
 +
<tr><td>Rv3037c</td><td>pSB1C3</td><td>Acyltransferase Candidate 4</td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618004" style = "color: #002bb8;">BBa_K1618004</a></td></tr>
  
 
</table>
 
</table>
 +
</body>
 +
 +
    <h2 class="title1">COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN</h2>
 +
    <div class="space10"></div>
 +
 +
 +
<table style="width:100%">
 +
<tr>
 +
    <th>Part Contents</th>
 +
    <th>Backbone</th>
 +
  <th>Description</th>
 +
<th>Function</th>
 +
  <th>Name in registry</th>
 +
  </tr>
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_GlgX(K1618025)</td><td>pSB1C3</td><td>Debranching Enzyme Expression Construct</td><td>Removes Glycogen Branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618026"  style = "color: #002bb8;">BBa_K1618026</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)</td><td>pSB1C3</td><td>Branching Enzyme Expression Construct</td><td>Adds Glycogen Branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618022" style = "color: #002bb8;">BBa_K1618022</a></td></tr>
 +
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_MAO(K1618001)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618005" style = "color: #002bb8;">BBa_K1618005</a> </td></tr>
 +
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618007" style = "color: #002bb8;">BBa_K1618007</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td> <td><a href="http://parts.igem.org/Part:BBa_K1618008" style = "color: #002bb8;">BBa_K1618008</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td> <td><a href="http://parts.igem.org/Part:BBa_K1618009" style = "color: #002bb8;">BBa_K1618009</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618023" style = "color: #002bb8;">BBa_K1618023</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618024" style = "color: #002bb8;">BBa_K1618024</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618027" style = "color: #002bb8;">BBa_K1618027</a></td></tr>
 +
 +
</table>
 +
 +
 +
<h2 class="title1">COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (Coding sequences from <I>Coprococcus sp. L2-50 </I> -DSM 16842-) </h2>
 +
    <div class="space10"></div>
 +
 +
 +
<table style="width:100%">
 +
<tr>
 +
    <th>Part Contents</th>
 +
    <th>Backbone</th>
 +
  <th>Description</th>
 +
<th>Function</th>
 +
  <th>Name in registry</th>
 +
  </tr>
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_Buk</td><td>pSB1C3</td><td>Butyrate Kinase Expression Contruct </td><td> Catalyzes the Conversion of Butyrate to/from Butyryl</td><td><a href="http://parts.igem.org/Part:BBa_K1618012" style = "color: #002bb8;">BBa_K1618012</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase Expression Construct</td><td>Catalyzes the Conversion of Butyryl to/from Butyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618013" style = "color: #002bb8;">BBa_K1618013</a></td></tr>
 +
 +
  <tr><td>P-Lac(R0010)_RBS(B0034)_Buk_PTB</td><td>pSB1C3</td><td>Butyrate to Butyryl-CoA Pathway</td><td> Catalyzes the Conversion of Butyryl to/from Butyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020" style = "color: #002bb8;">BBa_K1618020</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_THL</td><td>pSB1C3</td><td>Thiolase Expression Construct</td><td>Catalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618014" style = "color: #002bb8;">BBa_K1618014</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_CRO</td><td>pSB1C3</td><td>Crotonase Expression Construct</td><td>Catalyzes the Conversion of 3-hydroxybutyryl-CoA to Crotonyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618015" style = "color: #002bb8;">BBa_K1618015</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_BHBD</td><td>pSB1C3</td><td>β-hydroxybutyryl-CoA Dehydrogenase Expression</td><td>Catalyzes the Conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618016" style = "color: #002bb8;">BBa_K1618016</a></td></tr>
 +
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_BCD</td><td>pSB1C3</td><td>Butyryl-CoA Dehydrogenase Expression Construct</td><td> Catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618017" style = "color: #002bb8;">BBa_K1618017</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_EFTb</td><td>pSB1C3</td><td>Electron Transfer Protein &#946; Expression Construct</td><td>Catalyzes Conversion of Acetate to Butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618018" style = "color: #002bb8;">BBa_K1618018</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_EFTa</td><td>pSB1C3</td><td>Electron Transfer Protein &#945; Expression Construct</td><td>Catalyzes Conversion of Acetate to Butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618019" style = "color: #002bb8;">BBa_K1618019</a></td></tr>
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-CoA to Butryl-CoA Pathway</td><td>Catalyzes Conversion of Acetyl-CoA to Butryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618021" style = "color: #002bb8;">BBa_K1618021</a></td></tr>
 +
 +
 +
<tr><td>P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-CoA to Butyrate Complete Biosynthetic Pathway</td><td>Catalyzes Conversion of Acetyl-CoA to Butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618046" style = "color: #002bb8;">BBa_K1618046</a></td></tr>
 +
 +
 +
</table>
 +
 +
 +
<h2 class="title1">BASIC AND COMPOUND PARTS FOR ENGINEERING PLANT STARCH</h2>
 +
    <div class="space10"></div>
 +
 +
 +
<table style="width:100%">
 +
<tr>
 +
    <th>Part Contents</th>
 +
    <th>Backbone</th>
 +
  <th>Description</th>
 +
<th>Function</th>
 +
  <th>Name in registry</th>
 +
  </tr>
 +
 +
 +
 +
<tr><td>P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 1 Expression Construct</td><td>Transfers Acyl Groups to Starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618033" style = "color: #002bb8;">BBa_K1618033</a></td></tr>
 +
 +
 +
 +
<tr><td>P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 2 Expression Construct</td><td>Transfers Acyl Groups to Starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618034" style = "color: #002bb8;">BBa_K1618034</a></td></tr>
 +
 +
 +
 +
<tr><td>P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 3 Expression Construct</td><td>Transfers Acyl Groups to Starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618035" style = "color: #002bb8;">BBa_K1618035</a></td></tr>
 +
 +
 +
 +
<tr><td>P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 4 Expression Construct</td><td>Transfers Acyl Groups to Starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618036" style = "color: #002bb8;">BBa_K1618036</a></td></tr>
 +
 +
 +
<tr><td>P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 1 -Fused to GFP -Expression Construct </td><td>Check Localization in Plastid of Candidate Gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618029" style = "color: #002bb8;">BBa_K1618029</a></td></tr>
 +
 +
 +
<tr><td>P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 2 -Fused to GFP -Expression Construct </td><td>Check Localization in Plastid of Candidate Gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618030" style = "color: #002bb8;">BBa_K1618030</a></td></tr>
 +
 +
 +
<tr><td>P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 3 -Fused to GFP -Expression Construct </td><td>Check Localization in Plastid of Candidate Gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618031" style = "color: #002bb8;">BBa_K1618031</a></td></tr>
 +
 +
 +
<tr><td>P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 4 -Fused to GFP -Expression Construct </td><td>Check Localization in Plastid of Candidate Gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618032" style = "color: #002bb8;">BBa_K1618032</a></td></tr>
 +
 +
<tr><td>35s Terminator</td><td>pSB1C3**</td><td>Eukaryotic Terminator from the 35s Gene (Cauliflower Mosaic Virus)</td><td>Terminates Transcription in Plant Cells</td><td><a href="http://parts.igem.org/Part:BBa_K1618037" style = "color: #002bb8;">BBa_K1618037</a></td></tr>
 +
 +
 +
<tr><td>Chloroplast Transit Peptide</td><td>pSB1C3***</td><td>Synthetic Plastid Transit Peptide From the Consensus of Multiple RuBisCo Small Subunit Genes</td><td>In an N-terminal Fusion, this Peptide Directs Proteins to the Plant Plastid.</td><td><a href="http://parts.igem.org/Part:BBa_K1618028" style = "color: #002bb8;">BBa_K1618028</a></td></tr>
 +
</table>
 +
 +
<div class="space10"></div>
 +
<p>* This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcriptional unit flipper) made by the NRP-UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using <i>Agrobacterium tumefaciens</i> mediated transfection.</p>
 +
 +
<p>** This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (Terminator Flipper) made by the NRP-UEA 2014 team.</p>
 +
<p>*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone, according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick. </p>
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                     <h2 class="gray">MANY THANKS TO OUR SPONSORS</h2>
 
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Latest revision as of 10:23, 21 October 2015

House of Carbs

Parts

BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents Backbone Description Function Name in registry
GlgXpSB1C3Debranching Enzyme (E. coli)Coding sequence only BBa_K1618025
GlgBpSB1C3Branching Enzyme (E. coli)Coding sequence only BBa_K1618000
MAOpSB1C3Acyltransferase Candidate 1 Coding sequence only BBa_K1618001
Rv3030pSB1C3Acyltransferase Candidate 2Coding sequence onlyBBa_K1618002
Rv3034cpSB1C3Acyltransferase Candidate 3Coding sequence only BBa_K1618003
Rv3037cpSB1C3Acyltransferase Candidate 4Coding sequence only BBa_K1618004

COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents Backbone Description Function Name in registry
P-Lac(R0010)_RBS(B0034)_GlgX(K1618025)pSB1C3Debranching Enzyme Expression ConstructRemoves Glycogen BranchesBBa_K1618026
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)pSB1C3Branching Enzyme Expression ConstructAdds Glycogen BranchesBBa_K1618022
P-Lac(R0010)_RBS(B0034)_MAO(K1618001)pSB1C3Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618005
P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)pSB1C3Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618007
P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)pSB1C3Acyltransferase Expression ConstructTransfers Acyl Groups to Glycogen BBa_K1618008
P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)pSB1C3Acyltransferase Expression ConstructTransfers Acyl Groups to Glycogen BBa_K1618009
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)pSB1C3Branching Enzyme + Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)pSB1C3Branching Enzyme + Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618024
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)pSB1C3Branching Enzyme + Acyltransferase Expression ConstructTransfers Acyl Groups to GlycogenBBa_K1618027

COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (Coding sequences from Coprococcus sp. L2-50 -DSM 16842-)

Part Contents Backbone Description Function Name in registry
P-Lac(R0010)_RBS(B0034)_BukpSB1C3Butyrate Kinase Expression Contruct Catalyzes the Conversion of Butyrate to/from ButyrylBBa_K1618012
P-Lac(R0010)_RBS(B0034)_PTBpSB1C3Phosphotransbutrylase Expression ConstructCatalyzes the Conversion of Butyryl to/from Butyryl-CoABBa_K1618013
P-Lac(R0010)_RBS(B0034)_Buk_PTBpSB1C3Butyrate to Butyryl-CoA Pathway Catalyzes the Conversion of Butyryl to/from Butyryl-CoABBa_K1618020
P-Lac(R0010)_RBS(B0034)_THLpSB1C3Thiolase Expression ConstructCatalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA BBa_K1618014
P-Lac(R0010)_RBS(B0034)_CROpSB1C3Crotonase Expression ConstructCatalyzes the Conversion of 3-hydroxybutyryl-CoA to Crotonyl-CoA BBa_K1618015
P-Lac(R0010)_RBS(B0034)_BHBDpSB1C3β-hydroxybutyryl-CoA Dehydrogenase ExpressionCatalyzes the Conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA BBa_K1618016
P-Lac(R0010)_RBS(B0034)_BCDpSB1C3Butyryl-CoA Dehydrogenase Expression Construct Catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA BBa_K1618017
P-Lac(R0010)_RBS(B0034)_EFTbpSB1C3Electron Transfer Protein β Expression ConstructCatalyzes Conversion of Acetate to ButyrateBBa_K1618018
P-Lac(R0010)_RBS(B0034)_EFTapSB1C3Electron Transfer Protein α Expression ConstructCatalyzes Conversion of Acetate to ButyrateBBa_K1618019
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-CoA to Butryl-CoA PathwayCatalyzes Conversion of Acetyl-CoA to Butryl-CoA BBa_K1618021
P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-CoA to Butyrate Complete Biosynthetic PathwayCatalyzes Conversion of Acetyl-CoA to ButyrateBBa_K1618046

BASIC AND COMPOUND PARTS FOR ENGINEERING PLANT STARCH

Part Contents Backbone Description Function Name in registry
P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 1 Expression ConstructTransfers Acyl Groups to StarchBBa_K1618033
P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 2 Expression ConstructTransfers Acyl Groups to StarchBBa_K1618034
P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 3 Expression ConstructTransfers Acyl Groups to StarchBBa_K1618035
P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 4 Expression ConstructTransfers Acyl Groups to StarchBBa_K1618036
P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 1 -Fused to GFP -Expression Construct Check Localization in Plastid of Candidate GeneBBa_K1618029
P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 2 -Fused to GFP -Expression Construct Check Localization in Plastid of Candidate GeneBBa_K1618030
P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 3 -Fused to GFP -Expression Construct Check Localization in Plastid of Candidate GeneBBa_K1618031
P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037)pSB1C3*Acyltransferase Candidate 4 -Fused to GFP -Expression Construct Check Localization in Plastid of Candidate GeneBBa_K1618032
35s TerminatorpSB1C3**Eukaryotic Terminator from the 35s Gene (Cauliflower Mosaic Virus)Terminates Transcription in Plant CellsBBa_K1618037
Chloroplast Transit PeptidepSB1C3***Synthetic Plastid Transit Peptide From the Consensus of Multiple RuBisCo Small Subunit GenesIn an N-terminal Fusion, this Peptide Directs Proteins to the Plant Plastid.BBa_K1618028

* This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcriptional unit flipper) made by the NRP-UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using Agrobacterium tumefaciens mediated transfection.

** This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (Terminator Flipper) made by the NRP-UEA 2014 team.

*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone, according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick.

MANY THANKS TO OUR SPONSORS

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Contact Us

  • nrpuea.igem2015@gmail.com

  • Norwich Research Park,
  • Colney,
  • Norwich, NR4 7UH, UK.

We are the NRP-UEA-Norwich 2015 iGEM Team.

Designed and developed by the NRP-UEA-Norwich iGEM Team