Difference between revisions of "Team:NRP-UEA-Norwich/Parts"
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<li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Project</a> | <li class="dropdown " id="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Project</a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| + | <li ><a href="/Team:NRP-UEA-Norwich/Description">Overview</a></li> | ||
<li ><a href="/Team:NRP-UEA-Norwich/Project">Background</a></li> | <li ><a href="/Team:NRP-UEA-Norwich/Project">Background</a></li> | ||
<li ><a href="/Team:NRP-UEA-Norwich/Approach">Approach</a></li> | <li ><a href="/Team:NRP-UEA-Norwich/Approach">Approach</a></li> | ||
<li ><a href="/Team:NRP-UEA-Norwich/Results">Results</a></li> | <li ><a href="/Team:NRP-UEA-Norwich/Results">Results</a></li> | ||
| − | + | <li ><a href="/Team:NRP-UEA-Norwich/Parts">Parts</a></li> | |
| − | <li ><a href="/Team:NRP-UEA-Norwich/Protocols">Protocols | + | <li ><a href="/Team:NRP-UEA-Norwich/Protocols">Protocols</a></li> |
| − | + | ||
</ul> | </ul> | ||
| − | <li class="dropdown " id="dropdown"><a href="/Team:NRP-UEA-Norwich/ | + | <li class="dropdown " id="dropdown"><a href="/Team:NRP-UEA-Norwich/ModelingAndSoftware" class="dropdown-toggle">Modelling</a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li ><a href="/Team:NRP-UEA-Norwich/ | + | <li ><a href="/Team:NRP-UEA-Norwich/Software">Glyco2D</a></li> |
| − | + | ||
<li ><a href="/Team:NRP-UEA-Norwich/Modeling/3D">3D Models</a></li> | <li ><a href="/Team:NRP-UEA-Norwich/Modeling/3D">3D Models</a></li> | ||
| − | <li ><a href="/Team:NRP-UEA-Norwich/Modeling | + | <li ><a href="/Team:NRP-UEA-Norwich/Modeling">Kinetic Model</a></li> |
| + | |||
</ul> | </ul> | ||
</li> | </li> | ||
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<li ><a href="/Team:NRP-UEA-Norwich/Attributions">Attributions</a></li> | <li ><a href="/Team:NRP-UEA-Norwich/Attributions">Attributions</a></li> | ||
<li ><a href="/Team:NRP-UEA-Norwich/Practices">Practices</a></li> | <li ><a href="/Team:NRP-UEA-Norwich/Practices">Practices</a></li> | ||
| − | <li ><a href="/Team:NRP-UEA-Norwich/Collaborations">Collaborations</a></li> | + | <li ><a href="/Team:NRP-UEA-Norwich/Collaborations">Collaborations</a></li> |
| + | <li ><a href="/Team:NRP-UEA-Norwich/Achievements">Achievements</a></li> | ||
</li> | </li> | ||
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<h1 class="title1">Parts</h1> | <h1 class="title1">Parts</h1> | ||
<div class="space30"></div> | <div class="space30"></div> | ||
| + | <h2 class="title1">BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN</h2> | ||
| + | <div class="space10"></div> | ||
| − | |||
| − | |||
<style> | <style> | ||
table, th, td { | table, th, td { | ||
| Line 108: | Line 109: | ||
} | } | ||
</style> | </style> | ||
| − | |||
| − | |||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
| − | <th>Part | + | <th>Part Contents</th> |
<th>Backbone</th> | <th>Backbone</th> | ||
<th>Description</th> | <th>Description</th> | ||
<th>Function</th> | <th>Function</th> | ||
| − | <th> | + | <th>Name in registry</th> |
</tr> | </tr> | ||
| − | <tr><td> | + | <tr><td>GlgX</td><td>pSB1C3</td><td>Debranching Enzyme <i>(E. coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618025" style = "color: #002bb8;">BBa_K1618025</a></td></tr> |
| − | <tr><td> | + | <tr><td>GlgB</td><td>pSB1C3</td><td>Branching Enzyme <i>(E. coli)</i></td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618000" style = "color: #002bb8;">BBa_K1618000</a></td></tr> |
| − | + | <tr><td>MAO</td><td>pSB1C3</td><td>Acyltransferase Candidate 1 </td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618001" style = "color: #002bb8;">BBa_K1618001</a></td></tr> | |
| − | <tr><td> | + | <tr><td>Rv3030</td><td>pSB1C3</td><td>Acyltransferase Candidate 2</td><td>Coding sequence only</td><td><a href="http://parts.igem.org/Part:BBa_K1618002" style = "color: #002bb8;">BBa_K1618002</a></td></tr> |
| − | + | ||
| − | <tr><td> | + | <tr><td>Rv3034c</td><td>pSB1C3</td><td>Acyltransferase Candidate 3</td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618003" style = "color: #002bb8;">BBa_K1618003</a></td></tr> |
| − | + | ||
| − | |||
| − | |||
| − | <tr><td> | + | <tr><td>Rv3037c</td><td>pSB1C3</td><td>Acyltransferase Candidate 4</td><td>Coding sequence only</td> <td><a href="http://parts.igem.org/Part:BBa_K1618004" style = "color: #002bb8;">BBa_K1618004</a></td></tr> |
| − | + | </table> | |
| + | </body> | ||
| + | <h2 class="title1">COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN</h2> | ||
| + | <div class="space10"></div> | ||
| − | |||
| + | <table style="width:100%"> | ||
| + | <tr> | ||
| + | <th>Part Contents</th> | ||
| + | <th>Backbone</th> | ||
| + | <th>Description</th> | ||
| + | <th>Function</th> | ||
| + | <th>Name in registry</th> | ||
| + | </tr> | ||
| + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgX(K1618025)</td><td>pSB1C3</td><td>Debranching Enzyme Expression Construct</td><td>Removes Glycogen Branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618026" style = "color: #002bb8;">BBa_K1618026</a></td></tr> | ||
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)</td><td>pSB1C3</td><td>Branching Enzyme Expression Construct</td><td>Adds Glycogen Branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618022" style = "color: #002bb8;">BBa_K1618022</a></td></tr> |
| − | |||
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_MAO(K1618001)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618005" style = "color: #002bb8;">BBa_K1618005</a> </td></tr> |
| − | <tr><td> | + | |
| + | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618007" style = "color: #002bb8;">BBa_K1618007</a></td></tr> | ||
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td> <td><a href="http://parts.igem.org/Part:BBa_K1618008" style = "color: #002bb8;">BBa_K1618008</a></td></tr> |
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)</td><td>pSB1C3</td><td>Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td> <td><a href="http://parts.igem.org/Part:BBa_K1618009" style = "color: #002bb8;">BBa_K1618009</a></td></tr> |
| − | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618023" style = "color: #002bb8;">BBa_K1618023</a></td></tr> | |
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618024" style = "color: #002bb8;">BBa_K1618024</a></td></tr> |
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)</td><td>pSB1C3</td><td>Branching Enzyme + Acyltransferase Expression Construct</td><td>Transfers Acyl Groups to Glycogen</td><td><a href="http://parts.igem.org/Part:BBa_K1618027" style = "color: #002bb8;">BBa_K1618027</a></td></tr> |
| − | + | </table> | |
| − | |||
| − | + | <h2 class="title1">COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (Coding sequences from <I>Coprococcus sp. L2-50 </I> -DSM 16842-) </h2> | |
| + | <div class="space10"></div> | ||
| − | |||
| − | <tr><td> | + | <table style="width:100%"> |
| + | <tr> | ||
| + | <th>Part Contents</th> | ||
| + | <th>Backbone</th> | ||
| + | <th>Description</th> | ||
| + | <th>Function</th> | ||
| + | <th>Name in registry</th> | ||
| + | </tr> | ||
| + | <tr><td>P-Lac(R0010)_RBS(B0034)_Buk</td><td>pSB1C3</td><td>Butyrate Kinase Expression Contruct </td><td> Catalyzes the Conversion of Butyrate to/from Butyryl</td><td><a href="http://parts.igem.org/Part:BBa_K1618012" style = "color: #002bb8;">BBa_K1618012</a></td></tr> | ||
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase Expression Construct</td><td>Catalyzes the Conversion of Butyryl to/from Butyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618013" style = "color: #002bb8;">BBa_K1618013</a></td></tr> |
| − | + | <tr><td>P-Lac(R0010)_RBS(B0034)_Buk_PTB</td><td>pSB1C3</td><td>Butyrate to Butyryl-CoA Pathway</td><td> Catalyzes the Conversion of Butyryl to/from Butyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020" style = "color: #002bb8;">BBa_K1618020</a></td></tr> | |
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_THL</td><td>pSB1C3</td><td>Thiolase Expression Construct</td><td>Catalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618014" style = "color: #002bb8;">BBa_K1618014</a></td></tr> |
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_CRO</td><td>pSB1C3</td><td>Crotonase Expression Construct</td><td>Catalyzes the Conversion of 3-hydroxybutyryl-CoA to Crotonyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618015" style = "color: #002bb8;">BBa_K1618015</a></td></tr> |
| − | </tr> | + | |
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_BHBD</td><td>pSB1C3</td><td>β-hydroxybutyryl-CoA Dehydrogenase Expression</td><td>Catalyzes the Conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618016" style = "color: #002bb8;">BBa_K1618016</a></td></tr> |
| − | |||
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_BCD</td><td>pSB1C3</td><td>Butyryl-CoA Dehydrogenase Expression Construct</td><td> Catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618017" style = "color: #002bb8;">BBa_K1618017</a></td></tr> |
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_EFTb</td><td>pSB1C3</td><td>Electron Transfer Protein β Expression Construct</td><td>Catalyzes Conversion of Acetate to Butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618018" style = "color: #002bb8;">BBa_K1618018</a></td></tr> |
| − | <tr><td> | + | <tr><td>P-Lac(R0010)_RBS(B0034)_EFTa</td><td>pSB1C3</td><td>Electron Transfer Protein α Expression Construct</td><td>Catalyzes Conversion of Acetate to Butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618019" style = "color: #002bb8;">BBa_K1618019</a></td></tr> |
| + | <tr><td>P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-CoA to Butryl-CoA Pathway</td><td>Catalyzes Conversion of Acetyl-CoA to Butryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618021" style = "color: #002bb8;">BBa_K1618021</a></td></tr> | ||
| + | <tr><td>P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-CoA to Butyrate Complete Biosynthetic Pathway</td><td>Catalyzes Conversion of Acetyl-CoA to Butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618046" style = "color: #002bb8;">BBa_K1618046</a></td></tr> | ||
| + | </table> | ||
| + | <h2 class="title1">BASIC AND COMPOUND PARTS FOR ENGINEERING PLANT STARCH</h2> | ||
| + | <div class="space10"></div> | ||
| + | |||
| + | <table style="width:100%"> | ||
| + | <tr> | ||
| + | <th>Part Contents</th> | ||
| + | <th>Backbone</th> | ||
| + | <th>Description</th> | ||
| + | <th>Function</th> | ||
| + | <th>Name in registry</th> | ||
| + | </tr> | ||
| + | |||
| + | |||
| + | |||
| + | <tr><td>P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 1 Expression Construct</td><td>Transfers Acyl Groups to Starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618033" style = "color: #002bb8;">BBa_K1618033</a></td></tr> | ||
| + | |||
| + | |||
| + | |||
| + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 2 Expression Construct</td><td>Transfers Acyl Groups to Starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618034" style = "color: #002bb8;">BBa_K1618034</a></td></tr> | ||
| + | |||
| + | |||
| + | |||
| + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 3 Expression Construct</td><td>Transfers Acyl Groups to Starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618035" style = "color: #002bb8;">BBa_K1618035</a></td></tr> | ||
| + | |||
| + | |||
| + | |||
| + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 4 Expression Construct</td><td>Transfers Acyl Groups to Starch</td><td><a href="http://parts.igem.org/Part:BBa_K1618036" style = "color: #002bb8;">BBa_K1618036</a></td></tr> | ||
| + | |||
| + | |||
| + | <tr><td>P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 1 -Fused to GFP -Expression Construct </td><td>Check Localization in Plastid of Candidate Gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618029" style = "color: #002bb8;">BBa_K1618029</a></td></tr> | ||
| + | |||
| + | |||
| + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 2 -Fused to GFP -Expression Construct </td><td>Check Localization in Plastid of Candidate Gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618030" style = "color: #002bb8;">BBa_K1618030</a></td></tr> | ||
| + | |||
| + | |||
| + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 3 -Fused to GFP -Expression Construct </td><td>Check Localization in Plastid of Candidate Gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618031" style = "color: #002bb8;">BBa_K1618031</a></td></tr> | ||
| + | |||
| + | |||
| + | <tr><td>P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037)</td><td>pSB1C3*</td><td>Acyltransferase Candidate 4 -Fused to GFP -Expression Construct </td><td>Check Localization in Plastid of Candidate Gene</td><td><a href="http://parts.igem.org/Part:BBa_K1618032" style = "color: #002bb8;">BBa_K1618032</a></td></tr> | ||
| + | |||
| + | <tr><td>35s Terminator</td><td>pSB1C3**</td><td>Eukaryotic Terminator from the 35s Gene (Cauliflower Mosaic Virus)</td><td>Terminates Transcription in Plant Cells</td><td><a href="http://parts.igem.org/Part:BBa_K1618037" style = "color: #002bb8;">BBa_K1618037</a></td></tr> | ||
| + | |||
| + | |||
| + | <tr><td>Chloroplast Transit Peptide</td><td>pSB1C3***</td><td>Synthetic Plastid Transit Peptide From the Consensus of Multiple RuBisCo Small Subunit Genes</td><td>In an N-terminal Fusion, this Peptide Directs Proteins to the Plant Plastid.</td><td><a href="http://parts.igem.org/Part:BBa_K1618028" style = "color: #002bb8;">BBa_K1618028</a></td></tr> | ||
</table> | </table> | ||
| + | |||
| + | <div class="space10"></div> | ||
| + | <p>* This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcriptional unit flipper) made by the NRP-UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using <i>Agrobacterium tumefaciens</i> mediated transfection.</p> | ||
| + | |||
| + | <p>** This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (Terminator Flipper) made by the NRP-UEA 2014 team.</p> | ||
| + | <p>*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone, according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick. </p> | ||
| + | |||
| + | |||
| + | |||
</body> | </body> | ||
| Line 219: | Line 286: | ||
<h2 class="gray">MANY THANKS TO OUR SPONSORS</h2> | <h2 class="gray">MANY THANKS TO OUR SPONSORS</h2> | ||
<ul class="list-inline space30 icon"> | <ul class="list-inline space30 icon"> | ||
| − | <li | + | <li><img src="https://static.igem.org/mediawiki/2015/3/31/NRP-UEA-Norwich-Tgac.png" class="img-grey mautomargin"></li> |
| − | + | <li><img src="https://static.igem.org/mediawiki/2015/9/9e/NRP-UEA-Norwich-Nrp-logo.png" class="img-grey mautomargin" width="200" height="100"></a></li> | |
| − | + | <li><img src="https://static.igem.org/mediawiki/2015/e/ed/NRP-UEA-Norwich-UEAlogo.png" class="img-grey mautomargin" width="200" height="100"></a></li> | |
| − | + | <li><img src="https://static.igem.org/mediawiki/2015/9/9c/NRP-UEA-Norwich-Ibcarb_logo.png" width="200" height="100" class="img-grey mautomargin"></a></li> | |
| − | + | <li><img src="https://static.igem.org/mediawiki/2015/8/8b/NRP-UEA-Norwich-TSL-logo.png" width="200" height="100" class="img-grey mautomargin"></a></li> | |
| − | + | <li><img src="https://static.igem.org/mediawiki/2015/7/7d/NRP-UEA-Norwich-SEB-Logo.png" width="225" height="100" class="img-grey mautomargin"></a></li> | |
| − | + | <li><img src="https://static.igem.org/mediawiki/2015/d/d3/NRP-UEA-Norwich-JIC.png" width="200" height="100" class="img-grey mautomargin" width="200" height="100"></a></li> | |
| − | + | <li><img src="https://static.igem.org/mediawiki/2015/c/c5/NRP-UEA-Norwich-Bbsrc.png" width="200" height="100" class="img-grey mautomargin"></a></li> | |
| − | + | <li><img src="https://static.igem.org/mediawiki/2015/8/81/NRP-UEA-Norwich-Dna2.png" width="200" height="100" class="img-grey mautomargin"></a></li> | |
| − | <li | + | <li><img src="https://static.igem.org/mediawiki/2015/8/8e/NRP-UEA-Norwich-Sgm-logo.png" width="250" height="100" class="img-grey mautomargin"></a></li> |
| − | <li | + | <li><img src="https://static.igem.org/mediawiki/2015/e/e3/NRP-UEA-Norwich-Wellcome_Trust_logo.png" width="250" height="100" class="img-grey mautomargin"></a></li> |
| − | <li | + | <li><img src="https://static.igem.org/mediawiki/2015/7/74/NRP-UEA-Norwich-IBBA_logo.png" width="125" height="125" class="img-grey mautomargin"></a></li> |
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Latest revision as of 10:23, 21 October 2015
Parts
BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| GlgX | pSB1C3 | Debranching Enzyme (E. coli) | Coding sequence only | BBa_K1618025 |
| GlgB | pSB1C3 | Branching Enzyme (E. coli) | Coding sequence only | BBa_K1618000 |
| MAO | pSB1C3 | Acyltransferase Candidate 1 | Coding sequence only | BBa_K1618001 |
| Rv3030 | pSB1C3 | Acyltransferase Candidate 2 | Coding sequence only | BBa_K1618002 |
| Rv3034c | pSB1C3 | Acyltransferase Candidate 3 | Coding sequence only | BBa_K1618003 |
| Rv3037c | pSB1C3 | Acyltransferase Candidate 4 | Coding sequence only | BBa_K1618004 |
COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_GlgX(K1618025) | pSB1C3 | Debranching Enzyme Expression Construct | Removes Glycogen Branches | BBa_K1618026 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000) | pSB1C3 | Branching Enzyme Expression Construct | Adds Glycogen Branches | BBa_K1618022 |
| P-Lac(R0010)_RBS(B0034)_MAO(K1618001) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618005 |
| P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618007 |
| P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618008 |
| P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618009 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618024 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618027 |
COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (Coding sequences from Coprococcus sp. L2-50 -DSM 16842-)
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_Buk | pSB1C3 | Butyrate Kinase Expression Contruct | Catalyzes the Conversion of Butyrate to/from Butyryl | BBa_K1618012 |
| P-Lac(R0010)_RBS(B0034)_PTB | pSB1C3 | Phosphotransbutrylase Expression Construct | Catalyzes the Conversion of Butyryl to/from Butyryl-CoA | BBa_K1618013 |
| P-Lac(R0010)_RBS(B0034)_Buk_PTB | pSB1C3 | Butyrate to Butyryl-CoA Pathway | Catalyzes the Conversion of Butyryl to/from Butyryl-CoA | BBa_K1618020 |
| P-Lac(R0010)_RBS(B0034)_THL | pSB1C3 | Thiolase Expression Construct | Catalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA | BBa_K1618014 |
| P-Lac(R0010)_RBS(B0034)_CRO | pSB1C3 | Crotonase Expression Construct | Catalyzes the Conversion of 3-hydroxybutyryl-CoA to Crotonyl-CoA | BBa_K1618015 |
| P-Lac(R0010)_RBS(B0034)_BHBD | pSB1C3 | β-hydroxybutyryl-CoA Dehydrogenase Expression | Catalyzes the Conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA | BBa_K1618016 |
| P-Lac(R0010)_RBS(B0034)_BCD | pSB1C3 | Butyryl-CoA Dehydrogenase Expression Construct | Catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA | BBa_K1618017 |
| P-Lac(R0010)_RBS(B0034)_EFTb | pSB1C3 | Electron Transfer Protein β Expression Construct | Catalyzes Conversion of Acetate to Butyrate | BBa_K1618018 |
| P-Lac(R0010)_RBS(B0034)_EFTa | pSB1C3 | Electron Transfer Protein α Expression Construct | Catalyzes Conversion of Acetate to Butyrate | BBa_K1618019 |
| P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-CoA to Butryl-CoA Pathway | Catalyzes Conversion of Acetyl-CoA to Butryl-CoA | BBa_K1618021 |
| P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-CoA to Butyrate Complete Biosynthetic Pathway | Catalyzes Conversion of Acetyl-CoA to Butyrate | BBa_K1618046 |
BASIC AND COMPOUND PARTS FOR ENGINEERING PLANT STARCH
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 1 Expression Construct | Transfers Acyl Groups to Starch | BBa_K1618033 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 2 Expression Construct | Transfers Acyl Groups to Starch | BBa_K1618034 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 3 Expression Construct | Transfers Acyl Groups to Starch | BBa_K1618035 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 4 Expression Construct | Transfers Acyl Groups to Starch | BBa_K1618036 |
| P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 1 -Fused to GFP -Expression Construct | Check Localization in Plastid of Candidate Gene | BBa_K1618029 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 2 -Fused to GFP -Expression Construct | Check Localization in Plastid of Candidate Gene | BBa_K1618030 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 3 -Fused to GFP -Expression Construct | Check Localization in Plastid of Candidate Gene | BBa_K1618031 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 4 -Fused to GFP -Expression Construct | Check Localization in Plastid of Candidate Gene | BBa_K1618032 |
| 35s Terminator | pSB1C3** | Eukaryotic Terminator from the 35s Gene (Cauliflower Mosaic Virus) | Terminates Transcription in Plant Cells | BBa_K1618037 |
| Chloroplast Transit Peptide | pSB1C3*** | Synthetic Plastid Transit Peptide From the Consensus of Multiple RuBisCo Small Subunit Genes | In an N-terminal Fusion, this Peptide Directs Proteins to the Plant Plastid. | BBa_K1618028 |
* This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcriptional unit flipper) made by the NRP-UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using Agrobacterium tumefaciens mediated transfection.
** This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (Terminator Flipper) made by the NRP-UEA 2014 team.
*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone, according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick.
