Difference between revisions of "Team:UiOslo Norway"

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{{UiOslo_Norway}}
 
{{UiOslo_Norway}}
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<html>
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<html>
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<h2> Welcome to iGEM 2015! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h4>Before you start: </h4>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
 
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
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</ul>
 
  
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<div class="highlightBox">
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<h4> Styling your wiki </h4>
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<script type="text/javascript">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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var imagesToPreload = [
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  'https://static.igem.org/mediawiki/2015/9/97/UiOslo_Home_1_hover.jpg',
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  'https://static.igem.org/mediawiki/2015/b/ba/UiOslo_Home_2_hover.jpg',
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  'https://static.igem.org/mediawiki/2015/b/bc/UiOslo_Home_3_hover.jpg',
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];
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imagesToPreload.forEach(function(src) {
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  var img = new Image();
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  img.src = src;
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});
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</script>
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<style>
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<!--relative Angabe für die Überlappenden Module-->
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#fullpicture{
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    margin-left:auto;
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    margin-right:auto;
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    width: 312px
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}
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#fullpicture1{
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    background-image: url(https://static.igem.org/mediawiki/2015/f/fc/UiOslo_Home_1e.jpg);
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    height:342px;
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    width:312px;
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}
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#fullpicture1:hover{
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    background-image:url(https://static.igem.org/mediawiki/2015/9/97/UiOslo_Home_1_hover.jpg);
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}
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#fullpicture3{
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    background-image: url(https://static.igem.org/mediawiki/2015/0/0d/UiOslo_Home_2.jpg);
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    height:166px;
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    width:312px;
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}
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#fullpicture3:hover{
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    background-image:url(https://static.igem.org/mediawiki/2015/b/ba/UiOslo_Home_2_hover.jpg);
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}
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#fullpicture5{
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    background-image: url(https://static.igem.org/mediawiki/2015/f/f9/UiOslo_Home_3.jpg);
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    height:265px;
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    width:312px;
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}
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#fullpicture5:hover{
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    background-image:url(https://static.igem.org/mediawiki/2015/b/bc/UiOslo_Home_3_hover.jpg);
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}
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</style>
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<body>
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<div style="float:left; width:30%; padding: 0px 100px 0px 0px;">
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<div id="fullpicture">
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<a href="https://2015.igem.org/Team:UiOslo_Norway/Description#1">
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  <div id="fullpicture1">
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  </div>  
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</a>
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<a href="https://2015.igem.org/Team:UiOslo_Norway/Description#2">
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  <div id="fullpicture3">
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  </div>
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</a>
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<a href="https://2015.igem.org/Team:UiOslo_Norway/Description#3">
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  <div id="fullpicture5">
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  </div>
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</a>
 
</div>
 
</div>
  
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<h4> Editing your wiki </h4>
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</div>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:UiOslo_Norway&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
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<h4>Templates </h4>
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<div style="float:left; width:55%;">
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<p></br></br></p>
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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<h1 style="font-size:300%"> Methane Incorporated </h1>
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<p>(Hover over and click on the filterdrawing to get to the seperate subgoals.)</p>
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</br>
  
  
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<h4>Tips</h4>
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<p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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Methane  (CH<sub>4</sub>)  is  the second  most  prevalent  emitted  greenhouse  gas  on  earth.
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<ul>
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Today we are facing the challenge of solving this problem of high methane concentrations in the atmosphere that leads to global warming.  The  global  warming  potential  of  methane  is  about  84
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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times higher than that of carbon dioxide (CO<sub>2</sub>) when compared over 20 years. 
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<li>Be clear about what you are doing and how you plan to do this.</li>
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Technical methods to reuse emitted methane gas are cost- and time- intensive and therefore rarely used.
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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</p>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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The goal of our  project is  to  develop an <i>Escherichia coli</i> (<i>E. coli</i>)
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<li>Have lots of fun! </li>
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based  model  that filters methane out of the air and converts this into biomass. First part of the project is to break down methane to methanol with the enzyme complex soluble  methane  monooxygenase (sMMO)  of  the
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</ul>  
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methanotroph <i>Methylococcus  capsulatus</i> expressed in <i>E. coli</i>.  In  order  for  the  multisubunit
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enzyme complex to break down methane to methanol, oxygen and iron-ions are required. Second
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part of the project is to convert methanol into biomass using the Ribulose-Monophosphate (RuMP)- pathway and establish this pathway in  <i>E.coli.</i> The  pathway  is  found  in  the  methanotroph <i>Bacillus  methanolicus</i> and enables
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<i>E. coli</i> to convert methanol into biomass.
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</p>
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<p>
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To test the functionality of the modified <i>E. coli</i>, the bacteria will be grown in a closed
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system  under  methane  rich  conditions.  Additionally, a  filter  will be  created  that  can
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contain the modified <i>E.coli</i> and can filter the surrounding air through the system, so that the methane can be broken down. The filter could be used in areas where methane emission is a problem.
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</p>
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</div>
  
  
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<h4>Inspiration</h4>
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<div class="clear"></div>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<!---------------sponsors------------------------->
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
 
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
 
  
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<center><h1>iGEM UiOslo 2015 is sponsored by:</h1>
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<hr style="height:2px;border:none;color:black;background-color:black;" />
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<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/e/e0/UiOslo_uio_logo.png" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/6/65/UiOslo_Sponsor_Energi.png" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/4/4a/UiOslo_IDT.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
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</center>
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<hr style="height:2px;border:none;color:black;background-color:black;" />
  
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<!--------end sponsors---------------------------->
  
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</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
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</body>
  
 
</html>
 
</html>

Latest revision as of 08:11, 22 October 2015



Methane Incorporated

(Hover over and click on the filterdrawing to get to the seperate subgoals.)


Methane (CH4) is the second most prevalent emitted greenhouse gas on earth. Today we are facing the challenge of solving this problem of high methane concentrations in the atmosphere that leads to global warming. The global warming potential of methane is about 84 times higher than that of carbon dioxide (CO2) when compared over 20 years. Technical methods to reuse emitted methane gas are cost- and time- intensive and therefore rarely used.

The goal of our project is to develop an Escherichia coli (E. coli) based model that filters methane out of the air and converts this into biomass. First part of the project is to break down methane to methanol with the enzyme complex soluble methane monooxygenase (sMMO) of the methanotroph Methylococcus capsulatus expressed in E. coli. In order for the multisubunit enzyme complex to break down methane to methanol, oxygen and iron-ions are required. Second part of the project is to convert methanol into biomass using the Ribulose-Monophosphate (RuMP)- pathway and establish this pathway in E.coli. The pathway is found in the methanotroph Bacillus methanolicus and enables E. coli to convert methanol into biomass.

To test the functionality of the modified E. coli, the bacteria will be grown in a closed system under methane rich conditions. Additionally, a filter will be created that can contain the modified E.coli and can filter the surrounding air through the system, so that the methane can be broken down. The filter could be used in areas where methane emission is a problem.

iGEM UiOslo 2015 is sponsored by: