Latest revision as of 04:29, 29 October 2015

Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15
Week 16
Week 17
Week 18
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Lab Book

Before reading our lab notes, familiarize yourelf with our excel sheet, which specifies the nomenclature we've used in out project. You can also find all of our primers on this excel.

Week 1

Worked With	WorkFlow				May 18th - May 22nd
Preparations Plates	Making Cm plates	Stored in +4°C
Competent Cells	Preparing solutions for competent cells	Stored in +4°C
	o/n culture of E. coli TOP10	Chemical competent cells protocol	Transformation efficiency kit
	o/n culture of E. coli BL21(DE3)	Chemical competent cells protocol	Transformation efficiency kit	Colonies have the wrong color
Propagations	Transformed AH001-AH008 into TOP10	AH001-AH004 & AH006	Stored in +4°C
		No colonies for AH005, AH007 or AH008

Click here to read the full lab notes of week 1.

Week 2

Worked With	WorkFlow						May 25th - May 29th
Preparations Propagating	Transformed AH007-AH009, AH005 & AH019 into TOP10	AH007-AH009 & AH019	o/n cultures	MiniPrep
	o/n culture of AH001-AH004 & AH006	MiniPrep
	Re-transform AH005 into TOP10	Plates in rt over weekend

Checking Competent Cells	o/n culture of BL21(DE3) transformants	MiniPrep	PCR with VF2 & VR	Smeared gels

			AH001+ AH003+AH008 -> AH013	Repeat ligation	Restrict & extract from gel	Transform into TOP10	Plates in rt over weekend
Testing RBS's Assembly	Restrict AH001-AH004 & AH006-AH008	Ligation	AH002+ AH004+AH008 -> AH014	Repeat ligation	Restrict & extract from gel	Transform into TOP10	Plates in rt over weekend
			AH001+ AH006+AH008 -> AH011	Repeat ligation	Restrict & extract from gel	Transform into TOP10	Plates in rt over weekend

Click here to read the full lab notes of week 2.

Week 3

Worked With	WorkFlow						June 1st - June 5th
Preparations Propagating	Transform AH020 & AH021 into TOP10	Plates in rt over weekend

Testing RBS's Assembly	No colonies for AH011 or AH014	re-assemble	Restrict & extract from gel	Ligate & transform into TOP10	No colonies
	AH005 & AH013 OK	o/n cultures	MiniPrep
	Assembling AH015	Ligate AH002+ AH005+AH008	Restrict & extract from gel	Transform into TOP10	o/n cultures	No colonies on the plate

Click here to read the full lab notes of week 3.

Week 4

Worked With	WorkFlow						June 8th - June 12th
Preparations Propagating	o/n cultures for AH020 & AH021	MiniPrep

Testing RBS's Assembly	Re-assembling AH011, AH013 & AH014	Transform into TOP10	o/n cultures	MinPrep
	Assembling AH015	Ligate AH002+ AH005+AH008	Restrict & extract from gel	Transform into TOP10	o/n cultures	MiniPrep

Click here to read the full lab notes of week 4.

Week 5

Worked With	WorkFlow						June 15th - June 19th
						AH016 wrong size on gel
				Colonies in BL21(DE3)	o/n cultures & MiniPrep	AH018 OK on gel
Testing RBS's Assembly	Screening AH011 & AH013-AH015 by gel electrophoresis	AH011, AH013 & AH015 OK	Assembling AH016 & AH018	Transform into TOP10 & BL21(DE3)	T7 promoter doesn't work in TOP10...
		AH014 incorrect size	Assembling AH017 with multiple AH014 plasmids	Restrict & extract from gel	Transform into BL21(DE3)	Colonies are not blue
	Re-assembling AH016 & AH018	Restrict extract from gel	Transform into BL21(DE3)	Colonies are not blue

Preparations Linearized Backbones	o/n cultures of AH007-AH009	MiniPrep	Restrict & extract from gel	Low concentration

Click here to read the full lab notes of week 5.

Week 6

Worked With	WorkFlow				June 22nd - June 26th
Preparations Linearized Backbones	o/n cultures of AH007-AH009	MiniPrep	Restrict & extract from gel

Testing RBS's Assembly	Re-assembling AH016 & AH018	Transform into BL21(DE3)	No colonies
Propagation	o/n cultures of AH016 & AH018 in TOP10	MiniPrep	Transform into BL21(DE3)	Plates in rt over the weekend
Measurement	AH018 in BL21(DE3)	o/n culture	IPTG induction & blue chromoprotein measurement	No blue chromoprotein
		Refresh on IPTG plate	No blue chromoprotein

Click here to read the full lab notes of week 6.
Blue chromoprotein measurement data from 23.6. can be found here.

Week 7

Worked With	WorkFlow			June 29th - July 3rd
Preparations Linearized Backbones	Transform AH007-AH009 into TOP10	No colonies (mistake in protocol)	Use iGEM's linearized backbones

Testing RBS's	o/n cultures of AH016&AH018 in BL21	IPTG induction	No Blue chromoprotein
	Refresh AH016 & AH018 on IPTG plate	No blue chromoprotein

Violacein Plasmid Construction	PCR AH020 with P012&P013	PCR purification	VioA waits for 2nd PCR
	PCR AH20 with P024&P025	PCR unsuccesful
	PCR AH20 with P034&P035	PCR purification	VioE waits for 2nd PCR

Click here to read the full lab notes of week 7.
Blue chromoprotein measurement data from 30.6. can be found here.

Week 8

Worked With	WorkFlow					July 6th - July 10th
Preparations Competent Cells	Preparing PK BL21(DE3) competent cells	(Cells called PK's BL21(DE3))	Cells stored in -80°C

Cellulose Plasmid Construction	PCR AH021 with P019&P020	Problem with PCR machine	Re-do PCR	PCR purifcation	BglX waits for 2nd PCR
	AH029 into TOP10	Transformants stored in +4°C

Testing RBS's	Transform AH016 & AH018 into PK BL21(DE3)	Refresh transformants on IPTG plates	No blue chromoprotein

Violacein Plasmid Construction	Gradient PCR for AH020 with P024&P025	PCR purification

Click here to read the full lab notes of week 8.

Week 9

Worked With	WorkFlow						July 13th - July 17th
Preparations Propagations	o/n culture of AH029	MiniPrep

		CAR2 OK	PCR purification	Low yield
Propane Plasmid 1 Construction	Construct from 3 parts: CAR1-3	PCR for CAR1-3	CAR1 & 3 unspecific bands	Gradient PCR for CAR3	PCR with DMSO for CAR3	Store in -20°C
			PCR with DMSO for CAR1	No product

		PCR with DMSO for AtoB1	Store in -20°C
Propane Plasmid 2 Construction	Construct from 4 parts: AtoB1-4	PCR for AtoB 1-4	Gradient PCR for AtoB2	PCR with DMSO for AtoB2	Store in -20°C
		Gradient PCR for AtoB3	Extract & purify from gel	Store in -20°C
	Extract & purify AtoB4 from gel	Store in -20°C

GFP Brick Construction	o/n culture of AH044	MiniPrep

Click here to read the full lab notes of week 9.

Week 10

Worked With	WorkFlow						July 20th - July 24th
Preparations Linearized Backbones	Transform AH043 into TOP10	o/n culture	MiniPrep	Restrict
Propane Plasmid 1 Construction	Gradient PCR for CAR1	Extract & purify from gel	Low yield	TOPO Blunt End cloning and transform into TOP10t	Screen with colony PCR	Screen more colonies
	CAR2 PCR ran on gel	Gradient PCR	Extract & purify from gel	Illegal restriction site found in the part
	CAR3 PCR with DMSO run on gel	Extract purify from gel unclean product	TOPO Blunt End cloning and transform into TOP10t	Screen with colony PCR	Extract & purify from gel

Propane Plasmid 2 Construction	Run AtoB1 PCR with DMSO on gel	Extract & purify from gel	Low yield
	Run AtoB2 PCR with DMSO on gel	Extract & purify from gel	Low yield

Click here to read the full lab notes of week 10.

Week 11

Worked With	WorkFlow						July 27th - July 31st
Preparations Linearized Backbones	Re-restrict AH043	Gel purification	Low yield	Re-restrict
Propagations	o/n culture of AH031	MiniPrep
	Transform AH033 into TOP10	o/n culture	MiniPrep	Low yield	o/n culture	MiniPrep	Low yield, but stored in -20°C

Propane Plasmid 1 Construction	Continue CAR1 colony screening	Screen more colonies	PCR purification
	PCR for new CAR2	Gradient PCR with KAPA polymerase	PCR purification
	Re-do colony PCR for CAR3 successful TOPO colony	Re-do colony PCR
		o/n culture of the colony	MiniPrep	PCR
	KAPA gradient PCR for CAR3	Gel purification

Propane Plasmid 2 Construction	TOPO Blunt End Cloning for AtoB1 and transformation into TOP10	Screening with colony PCR	PCR purification
	KAPA gradient PCR for AtoB3&4	PCR purification for AtoB3
		Gel purification for AtoB4

Cellulose Plasmid Construction	2nd PCR for BglX (from AH021)	Stored in -4°C over the weekend
	PCR for Cen1 & Cen2	Gradient PCR with KAPA polymerase	PCR purification for Cen2
			Gel purification for Cen1

Violacein Plasmid Construction	2nd PCR for VioA (from AH020)	Stored in -4°C over the weekend

Click here to read the full lab notes of week 11.

Week 12

Worked With	WorkFlow						August 3rd - August 8th
Preparations Linearized Backbones	Check restricted AH043 on gel	Use for CAR OE-PCR
	Re-restrict with high amount of DNA	Extract & purify from gel	Low yield
	New o/n culture of AH043 (needed more backbone)	MiniPrep	Remove insert	Use for CAR NEBuilder
Fixing Commercial Backbones	Restrict and ligate AH006 into AH031&AH033	Transform into TOP10

Propane Plasmid 1 Construction	OE-PCR without backbone	Weak product band	PCR purification	Gradient PCR	No product
	OE-PCR with backbone	Transform into TOP10	Red colonies
	NEBuilder protocol	Transform into TOP10	Screen with colony PCR	o/n culture	MiniPrep	Send for sequencing

Violacein Plasmid Construction	PCR purification for VioA 2nd PCR	Stored in -20°C

Cellulose Plasmid Construction	PCR purification for BglX 2nd PCR	Stored in -20°C

Amph protein	Gradient PCR P044&P045, P046&P051, P045&P046	Extract & purify from gel P044&P045	DNA not clean	Store in -20°C
		Stored P046&P051 and P045&P046 product gels to +4°C

GFP protein	Gradient PCR with P028&P029	PCR purification

Propane Measurement	o/n culture of Pauli's propane strain	Propane measurement	No propane detected

Click here to read the full lab notes of week 12.

Week 13

Worked With	WorkFlow						August 10th - August 14th
Amphiphilic Protein Construction	PCR amph with P044&P045	No product	Gradient PCR	No product	Continue with last week's product
Amph with Linker Validation			Colony PCR amph with P045&P046	O/n culture	MiniPrep	Restrict & check on gel
	Extract & purify Amph P046&P051 and P045&P046 from gel	Assemble both into pSB1C3 backbone	Transform into TOP10
Amph no term Validation			Colony PCR amph with P046&P051	O/n culture	MiniPrep	Restrict & check on gel

Preparations Backbones	Restrict AH043	Stored in -20°C

GFP & Amph Bricks Assembly	Restrict & ligate GFP and Amph into AH009	Transform into TOP10	Screen with colony PCR	o/n culture	MiniPrep	Check on gel	Send for sequencing

Propane Plasmid 2 Assembly	NEBuilder protocol	Transform into TOP10	Screen with colony PCR	Re-do NEBuilder and transfrom to TOP10	Screen with colony PCR	o/n culture of one potential colony	o/n culture turned red
	ELIC protocol	Transform into TOP10	No colonies

Cellulose Plasmid Assembly	NEBuilder protocol and transform into TOP10	Screen with colony PCR	Mistake in protocol	Re-do NEBuilder and transform into TOP10	Screen with colony PCR	o/n cultures and MiniPrep of white colonies	Restrict & check on gel
	ELIC protocol	Transform into TOP10	Screen with colony PCR	Mistake in protocol	Re-do ELIC and transform into TOP10	Screen with colony PCR

GFP Brick Validation	Restrict AH013, AH015 & AH007	Ligate AH007, AH013 & GFP	Transform into TOP10	RT over weekend
		Ligate AH007, AH015 & GFP	Transform into TOP10	RT over weekend

Click here to read the full lab notes of week 13.

Week 14

Worked With	WorkFlow						August 17th - August 21st
Preparations Propagations	Transform AH045 into TOP10	o/n culture	MiniPrep

Propane Plasmid 1 Assembly	Restrict & ligate CAR into AH009	Transform into TOP10	o/n culture	MiniPrep	Restrict & check on gel
	Sequencing of Propane Plasmid 1 unsuccessful	Send construct in AH009 for sequencing
Validation	Transform into Pauli's BL21(DE3)

Amphiphilic Propane 1	Gradient PCR for CAR amph parts	CAR amph3 purified from gel
	PCR purification for CAR amph4

Propane Plasmid 2 Construction	OE-PCR	No product
	Check old NEBuilders on gel	Re-transform into TOP10	o/n cultures of colorless colonies	MiniPrep	Restrict & check on gel

Cellulose Plasmid Construction	OE-PCR	Extract & purify from gel	Concentration too low
		PCR purification	NEBuilder with AH009	Transform into NEB5alpha over weekend
	Check old NEBuilder on gel	Re-transform into TOP10	o/n of colorless colonies	MiniPrep	Restrict & check on gel

GFP Assembly	2A assembly for AH013/AH015, GFP & pSB1C3	Transform into TOP10	Colonies can’t be separated	3A assembly into pSB1T3	Transform into TOP10	No colonies
	Propagate GFP in AH009	MiniPrep	Send to Slovenia

Click here to read the full lab notes of week 14.

Week 15

Worked With	WorkFlow					August 24th - August 28th
Propane Plasmid 1 Validation	Prepare SDS-PAGE samples	Unclear results
	Analytical restriction	Wrong sized bands
	Prepared cells for purpald test	Stored in -70°C

Propane Plasmid 2 Construction	Repeat NEBuilder	Transform into TOP10
	Repeat NEBuilder with AH009	Transform into TOP10	Colony PCR

GFP Biobrick Assembly	3A Assemble GFP + AH045 in pSB1K3	Transform into TOP10	O/n cultues	MiniPrep	Restrict & Check on gel
	2A Assembly GFP + AH045 in pSB1K3	Transform into TOP10	Store in +4 °C

GFP+Amph Assembly	3A Assemble AH045-GFP + amphlinker in pSB1A3	Transform into TOP10 over weekend

Cellulose Plasmid Construction	Repeat NEBuilder	Transform into TOP10
	Repeat NEBuilder with AH009	Transform into TOP10	Colony PCR

Amphiphilic Propane1	NEBuilder	Transform into TOP10	Colony PCR
		O/n culture	MiniPrep

Click here to read the full lab notes of week 15.

Week 16

Worked With	WorkFlow						August 31st - September 4th
Propane Plasmid 1 Validation	New SDS-PAGE	Unclear results

Propane Plasmid 2 Construction	o/n culture of NEBuilder colonies	MiniPrep	Restrict & check on gel	No insert	Screen more colonies with colony PCR

Cellulose Plasmid Construction	o/n culture of NEBuilder colonies	MiniPrep	Restrict & check on gel	No insert	Screen more colonies with colony PCR

Amph Propane 1 Construction	Restrict & check on gel	Re-do NEBuilder	Transform into NEB5alpha	O/n cultures	MiniPrep

GFP Amph Assembly	O/n cultures	MiniPrep	Restrict & check on gel	O/n cultures of more colonies	MiniPrep, restrict & check on gel	Prepare samples for microscopy	Problem with imaging

Amphiphilic Brick Assembly	PCR purification	3A assemble AH045 + Amph in pSB1C3	Transform into TOP10	O/n cultures & MiniPrep	Restrict & Check on gel	More o/n cultures & MiniPrep	Restrict & Check on gel, results unclear

Click here to read the full lab notes of week 16.

Week 17

Worked With	WorkFlow						September 7th - September 11th
Propane Plasmid 1 Validation	Colony PCR with P001 & VR	Results next week

Propane Plasmid 2 Construction	o/n culture of NEBuilder colonies	MiniPrep	Restrict & check on gel	AtoB maybe OK	Restrict & check on gel again	Unclear results	Colony PCR, results next week

Cellulose Plasmid Construction	o/n culture of NEBuilder colonies	MiniPrep	Restrict & check on gel	No insert	Due to the time constraint, the construction is not repeated

Amph Propane 1 Construction	Restrict & check on gel	Incorrect size	Due to the time constraint, the construction is not repeated

GFP Amph Assembly	Restrict & check on gel	Incorrect size	Re-assemble	Transform into TOP10	GFP not expressed	Transform old ligation mix into TOP10	GFP not expressed
Validation	Imaged with fluorescent microscope
	O/w culture for microscopy

Amphiphilic Brick Assembly	Restrict & check on gel	Incorrect size	Re-assemble	Transform into TOP10	O/n cultures	MiniPrep
	O/w culture for microscopy

Click here to read the full lab notes of week 17.

Week 18

Worked With	WorkFlow						September 14th - September 18th
Propane Plasmid 1 Validation	Checking colony PCR results

Propane Plasmid 2 Construction	Checking colony PCR results

GFP Validation	Assembling AH046	Restriction and ligation	Transform into TOP10	No blue colonies	Re-assembling	Transform into TOP10	No blue colonies
	Assembling AH047	Restriction and ligation	Transform into TOP10	No blue colonies
GFP & Amph Validation	Prepare samples for confocal microscope	Pictures taken by VTT	Amphiphilic structures not detected
			GFP expression
Amph Brick Validation	Prepare samples for confocal microscope	Pictures taken by VTT	Amphiphilic structures not detected

Click here to read the full lab notes of week 18.

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-         <li><a href="#week1"><h4>Week 1</h4></a></li>
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-         <li><a href="#week2"><h4>Week 2</h4></a></li>
+         <li><a href="#week2" data-scroll="week2"><h4>Week 2</h4></a></li>
-         <li><a href="#week3"><h4>Week 3</h4></a></li>
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+         <li><a href="#week4" data-scroll="week4"><h4>Week 4</h4></a></li>
-         <li><a href="#week5"><h4>Week 5</h4></a></li>
+         <li><a href="#week5" data-scroll="week5"><h4>Week 5</h4></a></li>
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-         <li><a href="#week7"><h4>Week 7</h4></a></li>
+         <li><a href="#week7" data-scroll="week7"><h4>Week 7</h4></a></li>
-         <li><a href="#week8"><h4>Week 8</h4></a></li>
+         <li><a href="#week8" data-scroll="week8"><h4>Week 8</h4></a></li>
-         <li><a href="#week9"><h4>Week 9</h4></a></li>
+         <li><a href="#week9" data-scroll="week9"><h4>Week 9</h4></a></li>
-         <li><a href="#week10"><h4>Week 10</h4></a></li>
+         <li><a href="#week10" data-scroll="week10"><h4>Week 10</h4></a></li>
-         <li><a href="#week11"><h4>Week 11</h4></a></li>
+         <li><a href="#week11" data-scroll="week11"><h4>Week 11</h4></a></li>
-         <li><a href="#week12"><h4>Week 12</h4></a></li>
+         <li><a href="#week12" data-scroll="week12"><h4>Week 12</h4></a></li>
-         <li><a href="#week13"><h4>Week 13</h4></a></li>
+         <li><a href="#week13" data-scroll="week13"><h4>Week 13</h4></a></li>
-         <li><a href="#week14"><h4>Week 14</h4></a></li>
+         <li><a href="#week14" data-scroll="week14"><h4>Week 14</h4></a></li>
-         <li><a href="#week15"><h4>Week 15</h4></a></li>
+         <li><a href="#week15" data-scroll="week15"><h4>Week 15</h4></a></li>
-         <li><a href="#week16"><h4>Week 16</h4></a></li>
+         <li><a href="#week16" data-scroll="week16"><h4>Week 16</h4></a></li>
-         <li><a href="#week17"><h4>Week 17</h4></a></li>
+         <li><a href="#week17" data-scroll="week17"><h4>Week 17</h4></a></li>
-         <li><a href="#week18"><h4>Week 18</h4></a></li>
+         <li><a href="#week18" data-scroll="week18"><h4>Week 18</h4></a></li>
+         <li><a href="#"><h4 style="border-top:solid;">To the Top</h4></a></li>
+        <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Lab" ><h4>To the Parent Page</h4></a></li>
+      </ul>
-        <!-- There was code for drilldown menu, saving it for if I need it later. Does it work? Not sure... -->
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-            <li><a href="#">menu item 1-1</a></li>
-            <li><a href="#">menu item 1-2</a></li>
-            <li><a href="#">menu item 1-3</a></li>
-          </ul>
-          </div>
-        </li>-->
-      </ul>
 <div class="plasmids">
-<div class = "inner-container" >
+<div class = "inner-container" >
 <h1>Lab Book</h1>
+<p> Before reading our lab notes, familiarize yourelf with our <a href="https://static.igem.org/mediawiki/2015/7/7e/Aalto-Helsinki_plasmids-primers.xls">excel sheet</a>, which specifies the nomenclature we've used in out project. You can also find all of our primers on this excel.</p>
 </div><!-- end inner-container-->
-<table >
+<!-- Here the code for small horizontal gap for our tables, copy and paste where you want it Anna! --><!--
    <tr>
-    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td>
+         <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
-    <td class="bottomline"><h3>Things done</h3></td>
-    <td class="bottomline"></td>
-    <td class="bottomline"></td>
-    <td class="bottomline"></td>
-    <td class="bottomline"></td>
-    <td class="bottomline"></td>
-    <td class="bottomline"><h3>Lab 1.1.2015<br>Monday</h3></td>
-  </tr>
-  <tr>
-         <td class="first rightline"><div class="heightdiv"><h3>CAR</h3></div></td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p >Here is my text</p>
-        </td>
-        <td class="blue arrow" >
-            <p>Here is my text</p>
-        </td>
-	<td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="red arrow" >
-            <p>Here is my <br>text, there <br>is lot of it</p>
-        </td>
-  </tr>
-  <tr>
-        <td class="first rightline"><div class="heightdiv"><h3>another CAR?</h3></div></td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p ">Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-	<td class="blue arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="blue arrow" >
-            <p>Here is my <br>text, there <br>is lot of it</p>
-        </td>
-  </tr>
-  <tr>
-        <td class="first rightline"><div class="heightdiv"><h3>Ado?</h3></div></td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p >Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-	<td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="blue arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td  >
-        </td>
-        <td  >
-        </td>
-  </tr>
-    <tr>
-        <td class="first rightline"><div class="heightdiv"><h3>Ado cont.</h3></div></td>
-        <td  >
-        </td>
-        <td  >
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-	<td class="blue arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="white arrow" >
-            <p>Here is my text</p>
-        </td>
-        <td class="red arrow" >
-            <p>Here is my <br>text, there <br>is lot of it</p>
-        </td>
    </tr>
-</table>
+-->
-<h2 id="week1">Week 1</h2>
+<!-- Week 1 below -->
+<section id="week1" class="active" data-anchor="week1">
+<h2>Week 1</h2>
 <table >
@@ Line 250: / Line 156: @@
          <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td class="white arrow" >
-             <p>o/n culture <br/> of E. coli <br/> TOP10</p>
+             <p>o/n culture <br/> of <i>E. coli</i> <br/> TOP10</p>
          </td>
          <td class="white arrow" >
@@ Line 256: / Line 162: @@
          </td>
          <td class="blue arrow" >
-             <p>Tranformation <br/> efficiency kit</p>
+             <p>Transformation <br/> efficiency kit</p>
          </td>
    </tr>
@@ Line 262: / Line 168: @@
          <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td class="white arrow" >
-             <p>o/n culture <br/> of E. coli <br/> BL21(DE3)</p>
+             <p>o/n culture <br/> of <i>E. coli</i> <br/> BL21(DE3)</p>
          </td>
          <td class="white arrow" >
@@ Line 268: / Line 174: @@
          </td>
          <td class="red arrow" >
-             <p>Tranformation <br/> efficiency kit</p>
+             <p>Transformation <br/> efficiency kit</p>
          </td>
          <td class="red arrow" >
-             <p>Colonies are <br/> the wrong <br/>color</p>
+             <p>Colonies have<br/> the wrong <br/>color</p>
          </td>
    </tr>
@@ Line 299: / Line 205: @@
 </table>
+</section>
-<h2 id="week2">Week 2</h2>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/2/2b/Aalto-Helsinki_week1.pdf" target="_blank">here</a> to read the full lab notes of week 1.</p></div>
+<!-- Week 1 above -->
+<!-- Week 2 below -->
+<section id="week2" data-anchor="week2">
+<h2>Week 2</h2>
@@ Line 339: / Line 254: @@
    </tr>
    <tr>
-<td class="first rightline"><div class="heightdiv  bottomline"><h3></h3></div></td>
+<td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
-        <td>
-        </td>
-        <td class="reddown arrow" >
-            <p>No colonies <br/> for AH005</p>
-        </td>
          <td class="white arrow" >
-             <p>Re-transform <br/> into TOP10</p>
+             <p>Re-transform <br/> AH005<br/> into TOP10</p>
          </td>
          <td class="white arrow" >
@@ Line 354: / Line 264: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Checking <br/><span style="font-size:20px">Competent Cells</span></h3></div></td>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+         <td class="first rightline"><div class="heightdiv"><h3>Checking <br/><span style="font-size:20px">Competent Cells</span></h3></div></td>
          <td class="white arrow" >
              <p style="font-size:12px">o/n culture <br/> of BL21(DE3) <br/> transformants</p>
@@ Line 367: / Line 280: @@
              <p>&nbsp;&nbsp;Smeared gels</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
 <tr>
@@ Line 447: / Line 363: @@
 </table>
-<h2 id="week3">Week 3</h2>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/5/51/Aalto-Helsinki_week2.pdf" target="_blank">here</a> to read the full lab notes of week 2.</p></div>
+</section>
+<!-- Week 2 above -->
+<!-- Week 3 below -->
+<section id="week3" data-anchor="week3">
+<h2>Week 3</h2>
 <table >
@@ Line 461: / Line 386: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Preparations <br/><span style="font-size:20px">Propagating</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Preparations <br/><span style="font-size:20px">Propagating</span></h3></div></td>
          <td class="white arrow" >
              <p style="font-size:13px;">Transform <br/>&nbsp;&nbsp;&nbsp; AH020 & AH021 <br/> into TOP10</p>
@@ Line 468: / Line 393: @@
              <p>Plates in rt <br/> &nbsp;over weekend</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
    <tr>
@@ Line 482: / Line 410: @@
          </td>
          <td class="white arrow" >
-             <p>Ligate & <br/> tranform <br/> into TOP10</p>
+             <p>Ligate & <br/> transform <br/> into TOP10</p>
          </td>
          <td class="red arrow" >
@@ Line 526: / Line 454: @@
 </table>
-<h2 id="week4">Week 4</h2>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/7/70/Aalto-Helsinki_week3.pdf" target="_blank">here</a> to read the full lab notes of week 3.</p></div>
+</section>
+<!-- Week 3 above -->
+<!-- Week 4 below -->
+<section id="week4" data-anchor="week4">
+<h2>Week 4</h2>
 <table >
@@ Line 540: / Line 478: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Preparations <br/><span style="font-size:20px">Propagating</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Preparations <br/><span style="font-size:20px">Propagating</span></h3></div></td>
          <td class="white arrow" >
@@ Line 549: / Line 487: @@
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
    <tr>
@@ Line 591: / Line 532: @@
 </table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/1/1f/Aalto-Helsinki_week4.pdf" target="_blank">here</a> to read the full lab notes of week 4.</p></div>
-<h2 id="week5">Week 5</h2>
+</section>
+<!-- Week 4 above -->
+<!-- Week 5 below -->
+<section id="week5" data-anchor="week5">
+<h2>Week 5</h2>
 <table>
@@ Line 651: / Line 600: @@
              <p>Transform into TOP10 & BL21(DE3)</p>
          </td>
-         <td class="blue arrow" >
+         <td class="red arrow" >
              <p>T7 promoter <br/>doesn't work <br/>in TOP10...</p>
          </td>
@@ Line 676: / Line 625: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td class="white arrow" >
              <p>Re-assembling <br/>AH016 &<br/> AH018</p>
@@ Line 689: / Line 638: @@
              <p>Colonies are <br/> not blue</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
    <tr>
@@ Line 705: / Line 657: @@
          </td>
    </tr>
+</table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/f/f8/Aalto-Helsinki_week5.pdf" target="_blank">here</a> to read the full lab notes of week 5.</p></div>
+</section>
+<!-- Week 5 above -->
+<!-- Week 6 above -->
+<section id="week6" data-anchor="week6">
-</table>
+<h2>Week 6</h2>
-<h2 id="week5">Week 6</h2>
 <table >
@@ Line 726: / Line 684: @@
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Preparations <br/> <span style="font-size:20px">Linearized Backbones</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Preparations <br/> <span style="font-size:20px">Linearized Backbones</span></h3></div></td>
          <td class="white arrow" >
@@ Line 739: / Line 697: @@
              <p>Restrict & <br/>extract <br/>from gel</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
    <tr>
@@ Line 797: / Line 758: @@
 </table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/9/9c/Aalto-Helsinki_week6.pdf" target="_blank">here</a> to read the full lab notes of week 6. <br> Blue chromoprotein measurement data from 23.6. can be found <a href="https://static.igem.org/mediawiki/2015/6/63/Aalto-Helsinki_blue_chromoprotein_measurement_data_230615.xls" target="_blank">here</a>.</p></div>
-<h2 id="week7">Week 7</h2>
+</section>
+<!-- Week 6 above -->
+<!-- Week 7 below -->
+<section id="week7" data-anchor="week7">
+<h2>Week 7</h2>
@@ Line 813: / Line 783: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Preparations <br/> <span style="font-size:20px">Linearized Backbones</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Preparations <br/> <span style="font-size:20px">Linearized Backbones</span></h3></div></td>
          <td class="white arrow" >
              <p style="font-size:12px">Transform <br/>AH007-AH009 <br/> into TOP10</p>
@@ Line 823: / Line 793: @@
              <p>Use iGEM's <br/>linearized <br/>backbones</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
    <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Testing RBS's</h3></div></td>
-         <td class="red arrow" >
+         <td class="white arrow" >
-             <p style="color:red;font-size:12px">o/n cultures of <br/> &nbsp;&nbsp;AH016&AH018 <br/> in TOP10</p>
+             <p style="color:black;font-size:12px">o/n cultures of <br/> &nbsp;&nbsp;AH016&AH018 <br/> in BL21</p>
          </td>
-         <td class="red arrow" >
+         <td class="white arrow" >
-             <p style="color:red">IPTG <br/>induction</p>
+             <p style="color:black">IPTG <br/>induction</p>
          </td>
          <td class="red arrow" >
-             <p style="color:red">No Blue <br/> chromoprotein</p>
+             <p style="color:white">No Blue <br/> chromoprotein</p>
          </td>
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td class="white arrow" >
              <p style="font-size:12px">Refresh AH016 &<br/>AH018 on <br/> IPTG plate</p>
@@ Line 844: / Line 817: @@
              <p >No blue <br/> chromoprotein</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
      <tr>
@@ Line 849: / Line 825: @@
          <td class="white arrow" >
-             <p>PCR AH20 <br/>with<br/> P012&P013</p>
+             <p>PCR AH020 <br/>with<br/> P012&P013</p>
          </td>
          <td class="blue arrow" >
@@ Line 887: / Line 863: @@
-<h2 id="week8">Week 8</h2>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/6/65/Aalto-Helsinki_week7.pdf" target="_blank">here</a> to read the full lab notes of week 7. <br> Blue chromoprotein measurement data from 30.6. can be found <a href="https://static.igem.org/mediawiki/2015/a/a5/Aalto-Helsinki_blue_chromoprotein_measurement_data_300615.xls" target="_blank">here</a>.</p></div>
+</section>
+<!-- Week 7 above -->
+<!-- Week 8 below -->
+<section id="week8" data-anchor="week8">
+<h2>Week 8</h2>
 <table >
@@ Line 901: / Line 888: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Preparations <span style="font-size:20px">Competent Cells</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Preparations <span style="font-size:20px">Competent Cells</span></h3></div></td>
          <td class="white arrow" >
              <p style="font-size:12px">Preparing <br/>&nbsp;&nbsp;PK BL21(DE3)<br/> competent cells</p>
          </td>
+        <td class="white arrow" >
+            <p>(Cells <br/>called PK's <br/>BL21(DE3))</p>
+        </td>
          <td class="blue arrow" >
              <p>Cells stored <br/>in -80°C</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
    <tr>
@@ Line 928: / Line 921: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td class="white arrow" >
              <p>AH029 into <br/> TOP10</p>
@@ Line 935: / Line 928: @@
              <p>Transformants <br/>stored in <br/>+4°C</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Testing RBS's</h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Testing RBS's</h3></div></td>
          </td>
@@ Line 950: / Line 946: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
      <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Violacein Plasmid <br/><span style="font-size:20px"> Construction</span></h3></div></td>
@@ Line 963: / Line 962: @@
 </table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/4/43/Aalto-Helsinki_week8.pdf" target="_blank">here</a> to read the full lab notes of week 8.</p></div>
-<h2 id="week9">Week 9</h2>
+</section>
+<!-- Week 8 above -->
+<!-- Week 9 below -->
+<section id="week9" data-anchor="week9">
+<h2>Week 9</h2>
 <table >
@@ Line 978: / Line 987: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Preparations <br/> <span style="font-size:20px">Propagations</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Preparations <br/> <span style="font-size:20px">Propagations</span></h3></div></td>
          <td class="white arrow" >
@@ Line 987: / Line 996: @@
          </td>
    </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
    <tr>
 <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td></td>
          <td class="blueup arrow" >
              <p>CAR2 OK</p>
@@ Line 996: / Line 1,007: @@
          <td class="blue arrow" >
              <p>PCR <br/>purification</p>
+        </td>
+        <td class="red arrow" >
+            <p>Low yield</p>
          </td>
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmids 1&2 <span style="font-size:20px">Construction </span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <span style="font-size:20px">Construction </span></h3></div></td>
+<td class="white arrow" >
+            <p>Construct <br/>from 3 parts:<br/> CAR1-3</p>
+         </td>
 <td class="white arrow" >
-             <p>PCR for <br/> CAR1-3 & <br/> AtoB1-2</p>
+             <p>PCR for <br/> CAR1-3</p>
          </td>
          <td class="red arrow" >
-             <p style="font-size:12px">CAR1,3 & <br/>AtoB1-2 <br/> unspecific bands</p>
+             <p style="font-size:12px">CAR1 & 3 <br/> unspecific bands</p>
          </td>
          <td class="red arrow" >
-             <p>Gradient PCR <br/>for CAR3 & 4</p>
+             <p>Gradient PCR <br/>for CAR3</p>
+        </td>
+        <td class="blue arrow">
+            <p>PCR with <br/> DMSO <br/>for CAR3</p>
+        </td>
+        <td class="white arrow">
+            <p>Store <br/>in -20°C</p>
          </td>
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
-<td class="whitedown arrow" >
+        <td></td> <td></td>
-             <p style="font-size:12px">PCR with DMSO <br/>for CAR1, 3 <br/> & AtoB1, 2</p>
+        <td class="whitedown arrow" >
+             <p style="font-size:12px">PCR with DMSO <br/>for CAR1</p>
          </td>
          <td class="red arrow" >
-             <p style="color:red">dunnowhat</p>
+             <p>No product</p>
          </td>
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>GFP Brick <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+        <td></td>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="blueup arrow" >
+            <p>PCR with <br/>DMSO for <br/>AtoB1</p>
+        </td>
+        <td class="white arrow" >
+            <p>Store <br/> in -20°C</p>
+        </td>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2 <span style="font-size:20px">Construction</span></h3></div></td>
+<td class="white arrow">
+            <p>Construct from <br/>4 parts: <br/>AtoB1-4</p>
+        </td>
+<td class="red arrow">
+            <p>PCR for <br/>AtoB 1-4</p>
+        </td>
+        <td class="white arrow">
+            <p>Gradient PCR <br/> for AtoB2</p>
+        </td>
+        <td class="blue arrow">
+            <p>PCR with <br/>DMSO for <br/>AtoB2</p>
+        </td>
+        <td class="white arrow" >
+            <p>Store <br/> in -20°C</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td></td>
+<td class="bluedown arrow">
+            <p>Gradient PCR <br/> for AtoB3</p>
+        </td>
+        <td class="white arrow">
+            <p>Extract & <br/> purify from gel</p>
+        </td>
+        <td class="white arrow">
+            <p>Store <br/> in -20°C</p>
+        </td>
+  </tr>
+  <tr>
+      <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+<td class="bluedown arrow" >
+            <p>Extract & <br/> purify AtoB4 <br/>from gel</p>
+        </td>
+<td class="white arrow" >
+            <p>Store <br/> in -20°C</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+         <td class="first rightline"><div class="heightdiv"><h3>GFP Brick <span style="font-size:20px">Construction</span></h3></div></td>
 <td class="white arrow" >
@@ Line 1,033: / Line 1,111: @@
          </td>
    </tr>
-  <tr>
-        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2 <span style="font-size:20px">Construction</span></h3></div></td>
+</table>
-<td class="white arrow" >
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
-            <p>PCR for <br/>AtoB3-4</p>
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/2/28/Aalto-Helsinki_week9.pdf" target="_blank">here</a> to read the full lab notes of week 9.</p></div>
+</section>
+<!-- Week 9 above -->
+<!-- Week 10 below -->
+<section id="week10" data-anchor="week10">
+<h2>Week 10</h2>
+<table>
+<tr>
+    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td>
+    <td class="bottomline"><h3>WorkFlow</h3></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"><h3>July 20th -<br/> July 24th</h3></td>
+  </tr>
+   <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Preparations <span style="font-size:20px">Linearized Backbones</span></h3></div></td>
          </td>
+        <td class="white arrow" >
+            <p>Transform AH043 into TOP10</p>
+        </td>
+	<td class="white arrow" >
+            <p>o/n culture</p>
+        </td>
+        <td class=blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+	<td class="red arrow" >
+            <p>Restrict</p>
+        </td>
+</tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Gradient PCR <br/> for CAR1</p>
+        </td>
+        <td class="white arrow" >
+            <p>Extract & <br/> purify from gel</p>
+        </td>
          <td class="red arrow" >
-             <p>Unspecific<br/> bands</p>
+             <p>Low yield</p>
+        </td>
+        <td class="white arrow" >
+            <p style="font-size:12px">TOPO Blunt End <br/>cloning and <br/>transform into TOP10t</p>
+        </td>
+        <td class="red arrow" >
+            <p>Screen with <br/> colony PCR</p>
+        </td>
+        <td class="white arrow" >
+            <p>Screen more <br/> colonies</p>
+        </td>
+  </tr>
+    <tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>CAR2 PCR <br/>ran on gel</p>
          </td>
          <td class="white arrow" >
-             <p>AtoB 4 <br/>extracted <br/>from gel</p>
+             <p>Gradient PCR</p>
+        </td>
+        <td class="white arrow" >
+            <p>Extract & <br/> purify <br/> from gel</p>
+        </td>
+        <td class="red arrow" >
+            <p style="font-size:12px">Illegal restriction <br/>site found <br/>in the part</p>
+        </td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>CAR3 PCR <br/>with DMSO <br/>run on gel</p>
+        </td>
+	<td class="red arrow" >
+            <p>Extract  <br/> purify from gel <br/><span style="font-size:12px">unclean product</span></p>
+        </td>
+        <td class="white arrow" >
+        <p style="font-size:12px">TOPO Blunt End <br/>cloning and <br/>transform into TOP10t</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Screen with <br/> colony PCR</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Extract & <br/>purify <br/>from gel</p>
+        </td>
+</tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2 <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Run AtoB1 <br/>PCR with <br/>DMSO on gel</p>
+        </td>
+        <td class="white arrow" >
+            <p>Extract & <br/> purify from gel</p>
          </td>
          <td class="red arrow" >
-             <p>Low <br/>concentration</p>
+             <p>Low yield</p>
          </td>
    </tr>
    <tr>
          <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
-        <td></td>
-<td class="whitedown arrow" >
-            <p>Gradient <br/>PCR for <br/>AtoB3</p>
          </td>
-         <td class="blue arrow" >
+         <td class="white arrow" >
-             <p>Extract & <br/> purify <br/>from gel</p>
+             <p>Run AtoB2 <br/>PCR with <br/>DMSO on gel</p>
          </td>
+	<td class="white arrow" >
+            <p>Extract & <br/>purify from gel</p>
+        </td>
+	<td class="red arrow" >
+            <p>Low yield</p>
+        </td>
+</tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
 </table>
-<h2 id="week10">Week 10</h2>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/2/2b/Aalto-Helsinki_week10.pdf" target="_blank">here</a> to read the full lab notes of week 10.</p></div>
+</section>
+<!-- Week 10 above -->
+<!-- Week 11 below -->
+<section id="week11" data-anchor="week11">
-<h2 id="week11">Week 11</h2>
+<h2>Week 11</h2>
@@ Line 1,104: / Line 1,296: @@
 </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          </td>
@@ Line 1,129: / Line 1,321: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
    <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <span style="font-size:20px">Construction</span></h3></div></td>
@@ Line 1,163: / Line 1,358: @@
 </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td>
          </td>
@@ Line 1,176: / Line 1,371: @@
          </td>
 </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="blue arrow" >
+            <p style="font-size:12px">KAPA <br/>gradient PCR <br/>for CAR3</p>
+        </td>
+	<td class="white arrow" >
+            <p>Gel purification</p>
+        </td>
+</tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Propane Plasmid 2 <span style="font-size:20px">Construction</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2 <span style="font-size:20px">Construction</span></h3></div></td>
          </td>
@@ Line 1,191: / Line 1,399: @@
          </td>
 </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="blue arrow" >
+            <p style="font-size:12px">KAPA gradient <br/>PCR for AtoB3&4</p>
+        </td>
+	<td class="white arrow" >
+            <p>PCR purification <br/>for AtoB3</p>
+        </td>
+</tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td>
+        </td>
+        <td class="whitedown arrow" >
+            <p>Gel <br/> purification <br/> for AtoB4</p>
+        </td>
+</tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
      <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Cellulose Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
@@ Line 1,215: / Line 1,444: @@
 </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td>
          </td>
@@ Line 1,224: / Line 1,453: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
      <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Violacein Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
@@ Line 1,235: / Line 1,466: @@
          </td>
 </tr>
-    <tr>
-        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
-        <td class="blue arrow" >
-            <p style="font-size:12px">KAPA gradient <br/>PCR for AtoB3,4<br/> & CAR3</p>
-        </td>
-	<td class="white arrow" >
-            <p>Gel purification <br/>for AtoB4 <br/>& Car3</p>
-        </td>
-</tr>
-    <tr>
-        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
-        <td>
-        </td>
-        <td class="whitedown arrow" >
-            <p>PCR <br/> purification <br/> for AtoB3</p>
-        </td>
-</tr>
 </table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/1/15/Aalto-Helsinki_week11.pdf" target="_blank">here</a> to read the full lab notes of week 11.</p></div>
+</section>
+<!-- Week 11 above -->
+<!-- Week 12 below -->
+<section id="week12" data-anchor="week12">
-<h2 id="week12">Week 12</h2>
+<h2>Week 12</h2>
 <table >
@@ Line 1,306: / Line 1,530: @@
 </tr>
   <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3><span style="font-size:20px">Fixing Commercial Backbones</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3><span style="font-size:20px">Fixing Commercial Backbones</span></h3></div></td>
          <td class="white arrow" >
@@ Line 1,315: / Line 1,539: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
    <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <span style="font-size:20px">Construction</span></h3></div></td>
@@ Line 1,345: / Line 1,572: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td class="white arrow" >
              <p>NEBuilder <br/> protocol</p>
@@ Line 1,363: / Line 1,590: @@
              <p>Send for <br/>sequencing</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Violacein Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Violacein Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
          </td>
@@ Line 1,375: / Line 1,605: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>Cellulose Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>Cellulose Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
          <td class="blue arrow" >
-             <p>PCR purification <br/> for VioA <br/> 2nd PCR</p>
+             <p>PCR purification <br/> for BglX <br/> 2nd PCR</p>
          </td>
 	<td class="white arrow" >
@@ Line 1,385: / Line 1,618: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
      <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Amph protein</h3></div></td>
@@ Line 1,400: / Line 1,636: @@
          </td>
 </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td>
          </td>
@@ Line 1,408: / Line 1,645: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
     <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>GFP protein</h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>GFP protein</h3></div></td>
          <td class="blue arrow" >
@@ Line 1,418: / Line 1,658: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
      <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Propane <span style="font-size:20px">Measurement</span></h3></div></td>
@@ Line 1,428: / Line 1,670: @@
              <p>Propane measurement</p>
          </td>
-	<td class="white arrow" >
+	<td class="red arrow" >
-             <p>Results <br/>available <br/>next week</p>
+             <p>No propane <br/>detected</p>
          </td>
 </tr>
 </table>
-<h2 id="week13">Week 13</h2>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/a/a9/Aalto-Helsinki_week12.pdf" target="_blank">here</a> to read the full lab notes of week 12.</p></div>
+</section>
+<!-- Week 12 above -->
+<!-- Week 13 below -->
+<section id="week13" data-anchor="week13">
+<h2>Week 13</h2>
 <table >
@@ Line 1,448: / Line 1,700: @@
    </tr>
    <tr>
-        <td class="first rightline"><div class="heightdiv bottomline"><h3>Amphiphilic Protein <span style="font-size:20px">Construction</span></h3></div></td>
+       <td class="first rightline"><div class="heightdiv"><h3>Amphiphilic Protein <span style="font-size:20px">Construction</span></h3></div></td>
          <td class="white arrow" >
              <p>PCR amph <br/> with P044&P045</p>
@@ Line 1,461: / Line 1,713: @@
              <p>No product</p>
          </td>
-         <td class="red arrow" >
+         <td class="white arrow" >
-             <p style="color:red">dunnowhat</p>
+             <p>Continue with <br/>last week's <br/>product</p>
          </td>
+  </tr>
+<tr>
+       <td class="first rightline"><div class="heightdiv"><h3>Amph with Linker <span style="font-size:20px">Validation</span></h3></div></td>
+       <td></td><td></td>
+       <td class="blueup arrow" >
+            <p>Colony PCR <br/>amph with<br/> P045&P046</p>
+        </td>
+        <td class="white arrow" >
+            <p>O/n culture</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Restrict & <br/>check <br/>on gel</p>
+        </td>
+  </tr>
+  <tr>
+       <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+               <td class="blue arrow" >
+            <p style="font-size:12px">Extract & purify <br/>Amph P046&P051 <br/>and P045&P046 <br/>from gel</p>
+               <td class="white arrow" >
+            <p>Assemble both <br/>into pSB1C3 <br/>backbone</p>
+               <td class="blue arrow" >
+            <p>Transform <br/>into TOP10</p>
+ </tr>
+<tr>
+       <td class="first rightline"><div class="heightdiv"><h3>Amph no term <span style="font-size:20px">Validation</span></h3></div></td>
+      <td></td><td></td>
+<td class="bluedown arrow" >
+            <p>Colony PCR <br/>amph with<br/> P046&P051</p>
+        </td>
+        <td class="white arrow" >
+            <p>O/n culture</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Restrict & <br/>check <br/>on gel</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
      <tr>
-<td class="first rightline"><div class="heightdiv bottomline"><h3>Preparations <span style="font-size:20px">Backbones</span></h3></div></td>
+<td class="first rightline"><div class="heightdiv"><h3>Preparations <span style="font-size:20px">Backbones</span></h3></div></td>
          <td class="white arrow" >
              <p>Restrict AH043</p>
@@ Line 1,474: / Line 1,771: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
      <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3>GFP & Amph Bricks <br/><span style="font-size:20px">Assembly</span></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3>GFP & Amph Bricks <br/><span style="font-size:20px">Assembly</span></h3></div></td>
              <td class="white arrow" >
              <p style="font-size:12px">Restrict & <br/>ligate GFP and<br/> Amph into AH009</p>
@@ Line 1,498: / Line 1,798: @@
          </td>
 </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
   <tr>
          <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2 <span style="font-size:20px">Assembly</span></h3></div></td>
@@ Line 1,524: / Line 1,827: @@
 </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td class="white arrow" >
              <p>ELIC protocol</p>
@@ Line 1,534: / Line 1,837: @@
              <p>No colonies</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
    <tr>
@@ Line 1,560: / Line 1,866: @@
    </tr>
    <tr>
-         <td class="first rightline"><div class="heightdiv bottomline"><h3></h3></div></td>
+         <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
          <td class="white arrow">
              <p>ELIC <br/>protocol</p>
@@ Line 1,578: / Line 1,884: @@
              <p>Screen with <br/> colony PCR</p>
          </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
    </tr>
      <tr>
@@ Line 1,614: / Line 1,923: @@
 </table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/0/03/Aalto-Helsinki_week13.pdf" target="_blank">here</a> to read the full lab notes of week 13.</p></div>
+</section>
+<!-- Week 13 above -->
+<!-- Week 14 below -->
+<section id="week14" data-anchor="week14">
 <h2 id="week14">Week 14</h2>
+<table >
+  <tr>
+    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td>
+    <td class="bottomline"><h3>WorkFlow</h3></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"><h3>August 17th -<br/> August 21st</h3></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Preparations <br/> <span style="font-size:20px">Propagations</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Transform <br/>AH045 into <br/>TOP10</p>
+        </td>
+        <td class="white arrow" >
+            <p>o/n  culture</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <span style="font-size:20px">Assembly</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Restrict & <br/> ligate CAR <br/>into AH009</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform into TOP10</p>
+        </td>
+        <td class="white arrow" >
+            <p>o/n culture</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Restrict & <br/> check on gel</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="red arrow" >
+            <p style:"font-size:12px">Sequencing of <br/>Propane Plasmid 1 <br/>unsuccessful</p>
+        </td>
+        <td class="white arrow" >
+            <p style="font-size:12px">Send construct in AH009 for sequencing</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3><span style="font-size:20px">Validation</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Transform into <br/>Pauli's <br/> BL21(DE3)</p>
+        </td>
+  </tr>
+<tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Amphiphilic Propane 1</h3></div></td>
+        <td class="white arrow" >
+            <p>Gradient PCR <br/>for CAR amph <br/> parts</p>
+        </td>
+        <td class="blue arrow" >
+            <p>CAR amph3 <br/>purified <br/> from gel</p>
+        </td>
+</tr>
+<tr>
+        <td class="first rightline"><div class="heightdiv"></div></td>
+        <td class="whitedown arrow" >
+            <p>PCR purification <br/> for CAR <br/> amph4</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2<br/> <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>OE-PCR</p>
+        </td>
+        <td class="red arrow" >
+            <p>No product</p>
+        </td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>Check old <br/>NEBuilders<br/> on gel</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Re-transform <br/> into TOP10</p>
+        </td>
+        <td class="white arrow" >
+            <p>o/n cultures of <br/>colorless <br/>colonies</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="red arrow" >
+            <p>Restrict & <br/> check <br/> on gel</p>
+        </td>
+  </tr>
+<tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Cellulose Plasmid <span style="font-size:20px"> Construction </span></h3></div></td>
+        <td class="blue arrow" >
+            <p>OE-PCR</p>
+        </td>
+        <td class="white arrow" >
+            <p>Extract & <br/> purify <br/> from gel</p>
+        </td>
+        <td class="red arrow" >
+            <p>Concentration too low</p>
+        </td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td>
+        </td>
+        <td class="whitedown arrow" >
+            <p>PCR <br/>purification</p>
+        </td>
+        <td class="white arrow" >
+            <p>NEBuilder with AH009</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform into <br/>NEB5alpha <br/> over weekend</p>
+        </td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>Check old <br/>NEBuilder <br/>on gel</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Re-transform into TOP10</p>
+        </td>
+        <td class="white arrow" >
+            <p>o/n of colorless <br/> colonies</p>
+        </td>
+        <td class="white arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="red arrow" >
+            <p>Restrict & <br/> check <br/>on gel</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>GFP <span <style="font-size:20px"> Assembly</span></h3></div></td>
+        <td class="white arrow">
+            <p style="font-size:12px">2A assembly for <br/>AH013/AH015,<br/>GFP & pSB1C3</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform <br/>into TOP10</p>
+        </td>
+        <td class="red arrow" >
+            <p>Colonies can’t <br/>be separated</p>
+        </td>
+        <td class="white arrow" >
+            <p>3A assembly <br/>into pSB1T3</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform <br/> into TOP10</p>
+        </td>
+        <td class="red arrow" >
+            <p>No colonies</p>
+        </td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow">
+            <p>Propagate GFP in AH009</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="red arrow" >
+            <p>Send to Slovenia</p>
+        </td>
+  </tr>
+</table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/e/e3/Aalto-Helsinki_week14.pdf" target="_blank">here</a> to read the full lab notes of week 14.</p></div>
+</section>
+<!-- Week 14 above -->
+<!-- Week 15 below -->
+<section id="week15" data-anchor="week15">
 <h2 id="week15">Week 15</h2>
+<table >
+  <tr>
+    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td>
+    <td class="bottomline"><h3>WorkFlow</h3></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"><h3>August 24th -<br/> August 28th</h3></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <br/> <span style="font-size:20px">Validation</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Prepare <br/>SDS-PAGE <br/>samples</p>
+        </td>
+        <td class="red arrow" >
+            <p>Unclear <br/>results</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>Analytical <br/> restriction</p>
+        </td>
+        <td class="red arrow" >
+            <p>Wrong sized <br/>bands</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>Prepared <br/>cells for <br/>purpald test</p>
+        </td>
+        <td class="white arrow" >
+            <p>Stored <br/>in -70°C</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+ <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2<br/> <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Repeat <br/>NEBuilder</p>
+        </td>
+        <td class="red arrow" >
+            <p>Transform into TOP10</p>
+        </td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>Repeat <br/>NEBuilder <br/>with AH009</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform into TOP10</p>
+        </td>
+        <td class="red arrow" >
+            <p>Colony PCR</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>GFP Biobrick <span style="font-size:20px"> Assembly</span></h3></div></td>
+        <td class="white arrow" >
+            <p>3A Assemble <br/>GFP + AH045 <br/>in pSB1K3</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform <br/> into TOP10</p>
+        </td>
+        <td class="white arrow" >
+            <p>O/n cultues</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Restrict &<br/> Check <br/>on gel</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>2A Assembly <br/> GFP + AH045 in pSB1K3</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform <br/> into TOP10</p>
+        </td>
+        <td class="blue arrow" >
+            <p style="color:white">Store in +4 °C</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>GFP+Amph <span style="font-size:20px">Assembly</span></h3></div></td>
+        <td class="white arrow" >
+            <p style="font-size:12px">3A Assemble <br/>AH045-GFP + <br/>amphlinker in pSB1A3</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform <br/> into TOP10 <br/> over weekend</p>
+        </td>
+</tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Cellulose Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Repeat <br/>NEBuilder</p>
+        </td>
+        <td class="red arrow" >
+            <p>Transform into TOP10</p>
+        </td>
+  </tr>
+ <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>Repeat <br/>NEBuilder <br/> with AH009</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform into TOP10</p>
+        </td>
+        <td class="red arrow" >
+            <p>Colony PCR</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Amphiphilic Propane1</h3></div></td>
+        <td class="white arrow" >
+            <p>NEBuilder</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Transform <br/> into TOP10</p>
+        </td>
+        <td class="red arrow" >
+            <p>Colony PCR</p>
+        </td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td></td>
+        <td class="whitedown arrow" >
+            <p>O/n culture</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+  </tr>
+</table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/e/ec/Aalto-Helsinki_week15.pdf" target="_blank">here</a> to read the full lab notes of week 15.</p></div>
+</section>
+<!-- Week 15 above -->
+<!-- Week 16 below -->
+<section id="week16" data-anchor="week16">
 <h2 id="week16">Week 16</h2>
+<table >
+  <tr>
+    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td>
+    <td class="bottomline"><h3>WorkFlow</h3></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"><h3>August 31st -<br/> September 4th</h3></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <br/> <span style="font-size:20px">Validation</span></h3></div></td>
+        <td class="white arrow" >
+            <p>New <br/>SDS-PAGE</p>
+        </td>
+        <td class="red arrow" >
+            <p style="color:white">Unclear </br>results</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+<td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2<br/> <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>o/n culture <br/>of NEBuilder <br/>colonies</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="white arrow" >
+            <p>Restrict & <br/>check <br/>on gel</p>
+        </td>
+        <td class="red arrow" >
+            <p>No insert</p>
+        </td>
+        <td class="white arrow" >
+            <p>Screen more <br/>colonies with <br/>colony PCR</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Cellulose Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>o/n culture <br/>of NEBuilder <br/>colonies</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="white arrow" >
+            <p>Restrict & <br/>check <br/>on gel</p>
+        </td>
+        <td class="red arrow" >
+            <p>No insert</p>
+        </td>
+        <td class="white arrow" >
+            <p>Screen more <br/>colonies with <br/>colony PCR</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Amph Propane 1<span style="font-size:20px"> Construction </span></h3></div></td>
+        <td class="red arrow" >
+            <p>Restrict & <br/> check <br/> on gel</p>
+        </td>
+        <td class="white arrow" >
+            <p>Re-do <br/>NEBuilder</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform <br/>into <br/>NEB5alpha</p>
+        </td>
+        <td class="white arrow" >
+            <p>O/n cultures</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>GFP Amph <span style="font-size:20px"> Assembly </span></h3></div></td>
+        <td class="white arrow" >
+            <p>O/n cultures</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="red arrow" >
+            <p>Restrict & <br/> check<br/> on gel</p>
+        </td>
+        <td class="white arrow" >
+            <p>O/n cultures <br/>of more <br/> colonies</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep, restrict<br/> & check<br/> on gel</p>
+        </td>
+        <td class="white arrow" >
+            <p>Prepare samples<br/> for microscopy</p>
+        </td>
+        <td class="white arrow" >
+            <p>Problem with<br/> imaging</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Amphiphilic Brick <span style="font-size:20px"> Assembly</span></h3></div></td>
+        <td class="white arrow" >
+            <p>PCR purification</p>
+        </td>
+        <td class="white arrow" >
+            <p>3A assemble <br/>AH045 + Amph<br/> in pSB1C3</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform <br/> into TOP10</p>
+        </td>
+        <td class="blue arrow" >
+            <p>O/n cultures & MiniPrep</p>
+        </td>
+        <td class="red arrow" >
+            <p>Restrict &<br/> Check <br/>on gel</p>
+        </td>
+        <td class="blue arrow" >
+            <p>More o/n cultures & MiniPrep</p>
+        </td>
+        <td class="white arrow" >
+            <p>Restrict &<br/> Check on gel,<br/> results unclear</p>
+        </td>
+  </tr>
+</table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/b/b1/Aalto-Helsinki_week16.pdf" target="_blank">here</a> to read the full lab notes of week 16.</p></div>
+</section>
+<!-- Week 16 above -->
+<!-- Week 17 below -->
+<section id="week17" data-anchor="week17">
 <h2 id="week17">Week 17</h2>
+<table >
+  <tr>
+    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td>
+    <td class="bottomline"><h3>WorkFlow</h3></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"><h3>September 7th -<br/> September 11th</h3></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <br/> <span style="font-size:20px">Validation</span></h3></div></td>
+        <td class="white arrow" >
+            <p>Colony PCR <br/>with <br/>P001 & VR</p>
+        </td>
+        <td class="white arrow" >
+            <p>Results <br/>next week</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+<td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2<br/> <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>o/n culture <br/>of NEBuilder <br/>colonies</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="white arrow" >
+            <p>Restrict & <br/>check <br/>on gel</p>
+        </td>
+        <td class="white arrow" >
+            <p>AtoB maybe<br/> OK</p>
+        </td>
+        <td class="white arrow" >
+            <p>Restrict & <br/>check on <br/>gel again</p>
+        </td>
+        <td class="white arrow" >
+            <p>Unclear results</p>
+        </td>
+        <td class="white arrow" >
+            <p>Colony PCR,<br/> results <br/>next week</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Cellulose Plasmid <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="white arrow" >
+            <p>o/n culture <br/>of NEBuilder <br/>colonies</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+        </td>
+        <td class="white arrow" >
+            <p>Restrict & <br/>check <br/>on gel</p>
+        </td>
+        <td class="red arrow" >
+            <p>No insert</p>
+        </td>
+        <td class="white arrow" >
+            <p style="font-size:12px">Due to the time <br/>constraint, the <br/>construction is<br/> not repeated</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Amph Propane 1<span style="font-size:20px"> Construction </span></h3></div></td>
+        <td class="white arrow" >
+            <p>Restrict & <br/> check <br/> on gel</p>
+        </td>
+        <td class="red arrow" >
+            <p>Incorrect size</p>
+        </td>
+        <td class="white arrow" >
+            <p style="font-size:12px">Due to the time <br/>constraint, the <br/>construction is<br/> not repeated</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>GFP Amph <span style="font-size:20px"> Assembly </span></h3></div></td>
+        <td class="white arrow" >
+            <p>Restrict & <br/> check on gel</p>
+        </td>
+        <td class="red arrow" >
+            <p>Incorrect size</p>
+        </td>
+        <td class="white arrow" >
+            <p>Re-assemble</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Transform <br/> into TOP10</p>
+        </td>
+        <td class="white arrow" >
+            <p>GFP not <br/>expressed</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform old <br/>ligation mix <br/>into TOP10</p>
+        </td>
+        <td class="red arrow" >
+            <p>GFP not <br/>expressed</p>
+        </td>
+ </tr>
+        <td class="first rightline"><div class="heightdiv"><h3><span style="font-size:20px"> Validation </span></h3></div></td>
+        <td class="white arrow" >
+            <p>Imaged with<br/> fluorescent <br/>microscope</p>
+        </td>
+ </tr>
+  <tr>
+  <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+  <td class="white arrow">
+      <p>O/w culture<br/> for microscopy</p>
+  </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Amphiphilic Brick <span style="font-size:20px"> Assembly </span></h3></div></td>
+        <td class="white arrow" >
+            <p>Restrict & <br/> check on gel</p>
+        </td>
+        <td class="red arrow" >
+            <p>Incorrect size</p>
+        </td>
+        <td class="white arrow" >
+            <p>Re-assemble</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Transform <br/> into TOP10</p>
+        </td>
+        <td class="white arrow" >
+            <p>O/n cultures</p>
+        </td>
+        <td class="blue arrow" >
+            <p>MiniPrep</p>
+  </tr>
+  <tr>
+  <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+  <td class="white arrow">
+      <p>O/w culture<br/> for microscopy</p>
+  </td>
+  </tr>
+</table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/2/27/Aalto-Helsinki_week17.pdf" target="_blank">here</a> to read the full lab notes of week 17.</p></div>
+</section>
+<!-- Week 17 above -->
+<!-- Week 18 below -->
+<section id="week18" data-anchor="week18">
 <h2 id="week18">Week 18</h2>
+<table >
+  <tr>
+    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td>
+    <td class="bottomline"><h3>WorkFlow</h3></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"></td>
+    <td class="bottomline"><h3>September 14th -<br/> September 18th</h3></td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <br/> <span style="font-size:20px">Validation</span></h3></div></td>
+        <td class="blue arrow" >
+            <p>Checking <br/>colony PCR <br/>results</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+  <tr>
+<td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2<br/> <span style="font-size:20px">Construction</span></h3></div></td>
+        <td class="red arrow" >
+            <p>Checking <br/> colony PCR <br/>results</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td>
+  </tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>GFP <span style="font-size:20px">Validation </span></h3></div></td>
+        <td class="white arrow" >
+            <p>Assembling <br/>AH046</p>
+        </td>
+        <td class="white arrow" >
+            <p>Restriction<br/> and ligation</p>
+        </td>
+        <td class="blue arrow" >
+            <p>Transform <br/>into TOP10</p>
+        </td>
+        <td class="white arrow" >
+            <p>No blue <br/> colonies</p>
+        </td>
+        <td class="white arrow" >
+            <p>Re-assembling</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform<br/>into TOP10</p>
+        </td>
+        <td class="red arrow" >
+            <p>No blue <br/> colonies</p>
+        </td>
+ </tr>
+ <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td class="white arrow" >
+            <p>Assembling <br/>AH047</p>
+        </td>
+        <td class="white arrow" >
+            <p>Restriction<br/> and ligation</p>
+        </td>
+        <td class="white arrow" >
+            <p>Transform <br/>into TOP10</p>
+        </td>
+        <td class="red arrow" >
+            <p>No blue <br/> colonies</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>GFP & Amph <span style="font-size:20px">Validation</span></h3></div></td>
+        <td class="white arrow" >
+            <p <span style="font-size:12px">Prepare samples <br/>for confocal <br/>microscope</span></p>
+        </td>
+        <td class="white arrow" >
+            <p>Pictures <br/>taken by <br/>VTT</p>
+        </td>
+        <td class="red arrow" >
+            <p>Amphiphilic <br/>structures<br/> not detected</p>
+        </td>
+  </tr>
+</tr>
+    <tr>
+        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
+        <td></td>
+        <td></td>
+        <td class="bluedown arrow" >
+            <p>GFP <br/>expression</p>
+        </td>
+  </tr>
+  <tr>
+        <td class="first rightline"><div class="heightdiv"><h3>Amph Brick<span style="font-size:20px"></br>Validation</span></h3></div></td>
+        <td class="white arrow" >
+            <p <span style="font-size:12px">Prepare samples <br/>for confocal <br/>microscope</span></p>
+        </td>
+        <td class="white arrow" >
+            <p>Pictures <br/>taken by <br/>VTT</p>
+        </td>
+        <td class="red arrow" >
+            <p>Amphiphilic <br/>structures <br/>not detected</p>
+        </td>
+  </tr>
+</table>
+<div class="inner-container" style="margin: 5% 20% 5% 25%;">
+<p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/f/fc/Aalto-Helsinki_week18.pdf" target="_blank">here</a> to read the full lab notes of week 18.</p></div>
+</section>
+<!-- Week 18 above -->
+<p style="margin-bottom:0;padding-bottom:10%;"></p>
 </div><!-- Background end-->
 </div></div></body> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
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-</html>
+<!-- here scripts to make the site navigation beautiful and responsive. -->
+<!-- NOTE: if you copy this script to other pages you'll likely have to modify it to have .inner-container instead of .plasmids in the lower function!! -->
+<script>
+$('#sidenav a').on('click', function() {
+    var scrollAnchor = $(this).attr('data-scroll'),
+        scrollPoint = $('section[data-anchor="' + scrollAnchor + '"]').offset().top - 28;
+    $('body,html').animate({
+        scrollTop: scrollPoint
+    }, 500);
+    return false;
+})
+$(window).scroll(function() {
+    var windscroll = $(window).scrollTop();
+    if (windscroll >= 100) {
+        $('.plasmids section').each(function(i) {
+            if ($(this).position().top <= windscroll + 140) {
+                $('#sidenav a.active').removeClass('active');
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+        $('#sidenav a.active').removeClass('active');
+        $('#sidenav a:first').addClass('active');
+    }
+}).scroll();
+</script>

Difference between revisions of "Team:Aalto-Helsinki/LabBook"

Latest revision as of 04:29, 29 October 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 16

Week 17

Week 18

To the Top

To the Parent Page

Lab Book

Week 1

Worked With

WorkFlow

May 18th - May 22nd

Preparations Plates

Competent Cells

Propagations

Week 2

Worked With

WorkFlow

May 25th - May 29th

Preparations Propagating

Checking Competent Cells

Testing RBS's Assembly

Week 3

Worked With

WorkFlow

June 1st - June 5th

Preparations Propagating

Testing RBS's Assembly

Week 4

Worked With

WorkFlow

June 8th - June 12th

Preparations Propagating

Testing RBS's Assembly

Week 5

Worked With

WorkFlow

June 15th - June 19th

Testing RBS's Assembly

Preparations Linearized Backbones

Week 6

Worked With

WorkFlow

June 22nd - June 26th

Preparations Linearized Backbones

Testing RBS's Assembly

Propagation

Measurement

Week 7

Worked With

WorkFlow

June 29th - July 3rd

Preparations Linearized Backbones

Testing RBS's

Violacein Plasmid Construction

Week 8

Worked With

WorkFlow

July 6th - July 10th

Preparations Competent Cells

Cellulose Plasmid Construction

Testing RBS's

Violacein Plasmid Construction

Week 9

Worked With

May 18th -
May 22nd

Preparations
Plates

May 25th -
May 29th

Checking
Competent Cells

Testing RBS's
Assembly

June 1st -
June 5th

Preparations
Propagating

Testing RBS's
Assembly

June 8th -
June 12th

Preparations
Propagating

Testing RBS's
Assembly

June 15th -
June 19th

Testing RBS's
Assembly

Preparations
Linearized Backbones

June 22nd -
June 26th

Preparations
Linearized Backbones

Testing RBS's
Assembly

June 29th -
July 3rd

Preparations
Linearized Backbones

Violacein Plasmid
Construction

July 6th -
July 10th

Cellulose Plasmid
Construction

Violacein Plasmid
Construction

July 13th -
July 17th

Preparations
Propagations

July 20th -
July 24th

July 27th -
July 31st