Difference between revisions of "Team:Aalto-Helsinki/LabBook"
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<div class = "inner-container" > | <div class = "inner-container" > | ||
<h1>Lab Book</h1> | <h1>Lab Book</h1> | ||
+ | |||
+ | <p> Before reading our lab notes, familiarize yourelf with our <a href="https://static.igem.org/mediawiki/2015/7/7e/Aalto-Helsinki_plasmids-primers.xls">excel sheet</a>, which specifies the nomenclature we've used in out project. You can also find all of our primers on this excel.</p> | ||
</div><!-- end inner-container--> | </div><!-- end inner-container--> | ||
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<td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
− | <p>o/n culture <br/> of E. coli <br/> TOP10</p> | + | <p>o/n culture <br/> of <i>E. coli</i> <br/> TOP10</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
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</td> | </td> | ||
<td class="blue arrow" > | <td class="blue arrow" > | ||
− | <p> | + | <p>Transformation <br/> efficiency kit</p> |
</td> | </td> | ||
</tr> | </tr> | ||
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<td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
− | <p>o/n culture <br/> of E. coli <br/> BL21(DE3)</p> | + | <p>o/n culture <br/> of <i>E. coli</i> <br/> BL21(DE3)</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
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</td> | </td> | ||
<td class="red arrow" > | <td class="red arrow" > | ||
− | <p> | + | <p>Transformation <br/> efficiency kit</p> |
</td> | </td> | ||
<td class="red arrow" > | <td class="red arrow" > | ||
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</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
− | <p>Ligate & <br/> | + | <p>Ligate & <br/> transform <br/> into TOP10</p> |
</td> | </td> | ||
<td class="red arrow" > | <td class="red arrow" > | ||
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</table> | </table> | ||
+ | |||
+ | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
+ | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/1/15/Aalto-Helsinki_week11.pdf" target="_blank">here</a> to read the full lab notes of week 11.</p></div> | ||
</section> | </section> | ||
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<td class="blue arrow" > | <td class="blue arrow" > | ||
− | <p>PCR purification <br/> for | + | <p>PCR purification <br/> for BglX <br/> 2nd PCR</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
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</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
+ | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/a/a9/Aalto-Helsinki_week12.pdf" target="_blank">here</a> to read the full lab notes of week 12.</p></div> | ||
</section> | </section> | ||
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<p>Continue with <br/>last week's <br/>product</p> | <p>Continue with <br/>last week's <br/>product</p> | ||
</td> | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Amph with Linker <span style="font-size:20px">Validation</span></h3></div></td> | ||
+ | <td></td><td></td> | ||
+ | <td class="blueup arrow" > | ||
+ | <p>Colony PCR <br/>amph with<br/> P045&P046</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>O/n culture</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>MiniPrep</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>Restrict & <br/>check <br/>on gel</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
+ | <td class="blue arrow" > | ||
+ | <p style="font-size:12px">Extract & purify <br/>Amph P046&P051 <br/>and P045&P046 <br/>from gel</p> | ||
+ | <td class="white arrow" > | ||
+ | <p>Assemble both <br/>into pSB1C3 <br/>backbone</p> | ||
+ | <td class="blue arrow" > | ||
+ | <p>Transform <br/>into TOP10</p> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Amph no term <span style="font-size:20px">Validation</span></h3></div></td> | ||
+ | <td></td><td></td> | ||
+ | <td class="bluedown arrow" > | ||
+ | <p>Colony PCR <br/>amph with<br/> P046&P051</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>O/n culture</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>MiniPrep</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>Restrict & <br/>check <br/>on gel</p> | ||
+ | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
+ | |||
+ | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
+ | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/0/03/Aalto-Helsinki_week13.pdf" target="_blank">here</a> to read the full lab notes of week 13.</p></div> | ||
</section> | </section> | ||
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<td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <span style="font-size:20px">Assembly</span></h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <span style="font-size:20px">Assembly</span></h3></div></td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
− | <p>Restrict & <br/> | + | <p>Restrict & <br/> ligate CAR <br/>into AH009</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
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<td class="first rightline"><div class="heightdiv"></div></td> | <td class="first rightline"><div class="heightdiv"></div></td> | ||
<td class="whitedown arrow" > | <td class="whitedown arrow" > | ||
− | <p>PCR | + | <p>PCR purification <br/> for CAR <br/> amph4</p> |
</td> | </td> | ||
</tr> | </tr> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
+ | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/e/e3/Aalto-Helsinki_week14.pdf" target="_blank">here</a> to read the full lab notes of week 14.</p></div> | ||
</section> | </section> | ||
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<td class="first rightline"><div class="heightdiv"><h3>GFP Biobrick <span style="font-size:20px"> Assembly</span></h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3>GFP Biobrick <span style="font-size:20px"> Assembly</span></h3></div></td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
− | <p> | + | <p>3A Assemble <br/>GFP + AH045 <br/>in pSB1K3</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
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<td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
− | <p> | + | <p>2A Assembly <br/> GFP + AH045 in pSB1K3</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
<p>Transform <br/> into TOP10</p> | <p>Transform <br/> into TOP10</p> | ||
</td> | </td> | ||
− | <td class=" | + | <td class="blue arrow" > |
− | <p style="color: | + | <p style="color:white">Store in +4 °C</p> |
</td> | </td> | ||
</tr> | </tr> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
+ | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/e/ec/Aalto-Helsinki_week15.pdf" target="_blank">here</a> to read the full lab notes of week 15.</p></div> | ||
</section> | </section> | ||
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</td> | </td> | ||
<td class="blue arrow" > | <td class="blue arrow" > | ||
− | <p>MiniPrep, restrict & check on gel</p> | + | <p>MiniPrep, restrict<br/> & check<br/> on gel</p> |
</td> | </td> | ||
<td class="white arrow" > | <td class="white arrow" > | ||
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</table> | </table> | ||
+ | |||
+ | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
+ | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/b/b1/Aalto-Helsinki_week16.pdf" target="_blank">here</a> to read the full lab notes of week 16.</p></div> | ||
+ | |||
</section> | </section> | ||
<!-- Week 16 above --> | <!-- Week 16 above --> | ||
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<td class="bottomline"></td> | <td class="bottomline"></td> | ||
<td class="bottomline"></td> | <td class="bottomline"></td> | ||
− | <td class="bottomline"><h3> | + | <td class="bottomline"><h3>September 7th -<br/> September 11th</h3></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 2,542: | Line 2,605: | ||
</td> | </td> | ||
<td class="red arrow" > | <td class="red arrow" > | ||
− | <p | + | <p>GFP not <br/>expressed</p> |
</td> | </td> | ||
</tr> | </tr> | ||
Line 2,551: | Line 2,614: | ||
</td> | </td> | ||
</tr> | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
+ | <td class="white arrow"> | ||
+ | <p>O/w culture<br/> for microscopy</p> | ||
+ | </td> | ||
+ | </tr> | ||
<tr> | <tr> | ||
<td class="first rightline"><div class="heightdivlow"><h3></h3></div></td> | <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td> | ||
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<p>O/n cultures</p> | <p>O/n cultures</p> | ||
</td> | </td> | ||
+ | |||
+ | <td class="blue arrow" > | ||
+ | <p>MiniPrep</p> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
+ | <td class="white arrow"> | ||
+ | <p>O/w culture<br/> for microscopy</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | </table> | ||
+ | |||
+ | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
+ | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/2/27/Aalto-Helsinki_week17.pdf" target="_blank">here</a> to read the full lab notes of week 17.</p></div> | ||
+ | |||
+ | </section> | ||
+ | <!-- Week 17 above --> | ||
+ | |||
+ | |||
+ | <!-- Week 18 below --> | ||
+ | <section id="week18" data-anchor="week18"> | ||
+ | |||
+ | <h2 id="week18">Week 18</h2> | ||
+ | |||
+ | <table > | ||
+ | <tr> | ||
+ | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Worked With</h3></td> | ||
+ | <td class="bottomline"><h3>WorkFlow</h3></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"><h3>September 14th -<br/> September 18th</h3></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 1 <br/> <span style="font-size:20px">Validation</span></h3></div></td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>Checking <br/>colony PCR <br/>results</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Propane Plasmid 2<br/> <span style="font-size:20px">Construction</span></h3></div></td> | ||
+ | |||
+ | <td class="red arrow" > | ||
+ | <p>Checking <br/> colony PCR <br/>results</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdivlow"><h3></h3></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>GFP <span style="font-size:20px">Validation </span></h3></div></td> | ||
+ | |||
+ | <td class="white arrow" > | ||
+ | <p>Assembling <br/>AH046</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Restriction<br/> and ligation</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>Transform <br/>into TOP10</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>No blue <br/> colonies</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Re-assembling</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Transform<br/>into TOP10</p> | ||
+ | </td> | ||
+ | <td class="red arrow" > | ||
+ | <p>No blue <br/> colonies</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
<td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
− | <td> | + | <td class="white arrow" > |
+ | <p>Assembling <br/>AH047</p> | ||
</td> | </td> | ||
− | <td> | + | <td class="white arrow" > |
+ | <p>Restriction<br/> and ligation</p> | ||
</td> | </td> | ||
− | <td> | + | <td class="white arrow" > |
+ | <p>Transform <br/>into TOP10</p> | ||
</td> | </td> | ||
− | <td> | + | <td class="red arrow" > |
+ | <p>No blue <br/> colonies</p> | ||
</td> | </td> | ||
− | <td class=" | + | </tr> |
− | <p style="font-size:12px"> | + | <tr> |
+ | <td class="first rightline"><div class="heightdiv"><h3>GFP & Amph <span style="font-size:20px">Validation</span></h3></div></td> | ||
+ | |||
+ | |||
+ | <td class="white arrow" > | ||
+ | <p <span style="font-size:12px">Prepare samples <br/>for confocal <br/>microscope</span></p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Pictures <br/>taken by <br/>VTT</p> | ||
</td> | </td> | ||
<td class="red arrow" > | <td class="red arrow" > | ||
− | <p | + | <p>Amphiphilic <br/>structures<br/> not detected</p> |
</td> | </td> | ||
</tr> | </tr> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
+ | |||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td class="bluedown arrow" > | ||
+ | <p>GFP <br/>expression</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Amph Brick<span style="font-size:20px"></br>Validation</span></h3></div></td> | ||
+ | |||
+ | <td class="white arrow" > | ||
+ | <p <span style="font-size:12px">Prepare samples <br/>for confocal <br/>microscope</span></p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Pictures <br/>taken by <br/>VTT</p> | ||
+ | </td> | ||
+ | <td class="red arrow" > | ||
+ | <p>Amphiphilic <br/>structures <br/>not detected</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
</table> | </table> | ||
− | |||
− | |||
− | |||
+ | <div class="inner-container" style="margin: 5% 20% 5% 25%;"> | ||
+ | <p style="font-size:16px">Click <a href="https://static.igem.org/mediawiki/2015/f/fc/Aalto-Helsinki_week18.pdf" target="_blank">here</a> to read the full lab notes of week 18.</p></div> | ||
− | |||
− | |||
− | |||
</section> | </section> | ||
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+ | |||
+ | |||
+ | <p style="margin-bottom:0;padding-bottom:10%;"></p> | ||
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Latest revision as of 04:29, 29 October 2015