Difference between revisions of "Team:Aalto-Helsinki/Results"
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<h3>Background</h3> | <h3>Background</h3> | ||
− | <p>Microbially produced propane holds enormous promise as a potential replacement of portable fossil fuels, but the propane yields with current biological pathways are low. | + | <p>Microbially produced propane holds enormous promise as a potential replacement of portable fossil fuels, but the propane yields with current biological pathways are low. Our pathway starts from acetyl-CoA and produces propane through 6 intermediates. To help concentrate engineering efforts on its critical parts, better quantitative understanding of the pathway is required. Our goals were to build a mathematical model of the pathway to better understand it and create and test BioBricks of the propane pathway to help future teams and researchers to continue improving it.</p> |
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<li><p>Improved our experimental plans according to the modeling results by changing one enzyme to a better homolog and expressing the rate-limiting enzyme from the highest copy number backbone used</p></li> | <li><p>Improved our experimental plans according to the modeling results by changing one enzyme to a better homolog and expressing the rate-limiting enzyme from the highest copy number backbone used</p></li> | ||
<li><p>Found that propane output was sensitive to NADPH/NADH, suggesting their efficient regeneration might be a limiting factor</p></li> | <li><p>Found that propane output was sensitive to NADPH/NADH, suggesting their efficient regeneration might be a limiting factor</p></li> | ||
− | <li><p>Designed two BioBricks containing all ten genes required for the propane pathway to produce propane in E. coli | + | <li><p>Designed two BioBricks containing all ten genes required for the propane pathway to produce propane in <i>E. coli</i> |
<ul> | <ul> | ||
<li><p>Chose three intercompatible backbones to ensure that the propane pathway could be integrated to the same cell with cellulose hydrolysis (which required the third backbone)</p></li> | <li><p>Chose three intercompatible backbones to ensure that the propane pathway could be integrated to the same cell with cellulose hydrolysis (which required the third backbone)</p></li> | ||
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<li><p><a href="https://2015.igem.org/Team:Aalto-Helsinki/Design">First report ever</a> worldwide of successful microbial production of propane in continuous production</p></li> | <li><p><a href="https://2015.igem.org/Team:Aalto-Helsinki/Design">First report ever</a> worldwide of successful microbial production of propane in continuous production</p></li> | ||
<li><p>145 hour continuous production experiment in a 500 ml chemostat</p></li> | <li><p>145 hour continuous production experiment in a 500 ml chemostat</p></li> | ||
− | <li><p>Propane yield 22 | + | <li><p>Propane yield up to 22.7 µg/L in reactor gas phase</p></li> |
</ul> | </ul> | ||
Latest revision as of 04:31, 29 October 2015