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| <ul> | | <ul> |
| <li><a href="https://2015.igem.org/Team:UChile-OpenBio">HOME</a></li> | | <li><a href="https://2015.igem.org/Team:UChile-OpenBio">HOME</a></li> |
− | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Team">TEAM</a></li> | + | <li> |
− | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Description">PROJECT</a></li> | + | <a href="https://2015.igem.org/Team:UChile-OpenBio/Description">PROJECT</a> |
− | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Experiments">EXPERIMENT</a></li> | + | <ul> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Description">PROJECT DESCRIPTION</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Experiments">EXPERIMENT AND PROTOCOLS</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Results">RESULTS</a></li> |
| + | </ul> |
| + | </li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Notebook">NOTEBOOK</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Atributions">ATRIBUTIONS</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Collaborations">COLLABORATION</a></li> |
| <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Practices">HUMAN PRACTICES</a></li> | | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Practices">HUMAN PRACTICES</a></li> |
− | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Collaborations">COLLABORATIONS</a></li> | + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Safety">SAFETY</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Modeling">MODELING</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Team">TEAM</a></li> |
| </ul> | | </ul> |
| </nav> | | </nav> |
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| <html> | | <html> |
| <!-- SI EXPORT END --> | | <!-- SI EXPORT END --> |
− | <section class="collaborations"> | + | <!-- ANTIGUA COLLABORATIONS --> |
− | <span class="titulo_seccion">Collaboration</span> | + | <section class="atributions"> |
− | <img id="activities_title" class="" alt="Activities" src="https://static.igem.org/mediawiki/2015/0/05/Activities_title.png" /> | + | <span class="titulo_seccion">Atributions</span> |
| + | <img id="activities_title" class="" alt="Activities" src="https://static.igem.org/mediawiki/2015/f/f6/Inicio.png" /> |
| + | <!--<img id="activities_title" class="" alt="Activities" src="https://static.igem.org/mediawiki/2015/0/05/Activities_title.png" />--> |
| + | |
| <article> | | <article> |
| <div class="division"> | | <div class="division"> |
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| <div class="item_actividad-titulo"><span>December 2014:</span><span>Team Formation</span></div> | | <div class="item_actividad-titulo"><span>December 2014:</span><span>Team Formation</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>At that time there was an organized group from <b> <span style="color:#CC0000"> Universidad de Chile </span></b> called <b> <span style="color:#CC0000"> OpenBio </span></b>. That team was funded on December 2013 by <b> <span style="color:#CC0000"> Luis Rodriguez </span></b>and <b><span style="color:#CC0000">Pablo Troncoso</span></b> (we miss you!). Organization’s main objectives are:<br/> | + | <p>At that time there was an organized group from <b> <span style="color:#CC0000"> Universidad de Chile </span></b> called <b> <span style="color:#CC0000"> OpenBio </span></b>. That team was founded on December 2013 by <b> <span style="color:#CC0000"> Luis Rodriguez </span></b>and <b><span style="color:#CC0000">Pablo Troncoso</span></b> (we miss you!). Organization’s main objectives are:<br/> |
| 1.- Boosting Synthetic Biology in Chile. <br/> | | 1.- Boosting Synthetic Biology in Chile. <br/> |
| 2.- Development of low cost Biotechnologies machines.<br/> | | 2.- Development of low cost Biotechnologies machines.<br/> |
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| <p>Thanks to the activities that the group developed and others that were attended by them, was possible to contact with people interested in being part of the project and very committed to the tasks involved in developing it.</p> | | <p>Thanks to the activities that the group developed and others that were attended by them, was possible to contact with people interested in being part of the project and very committed to the tasks involved in developing it.</p> |
| <img class="" alt="Team Formation2" src="https://static.igem.org/mediawiki/2015/6/60/Team_formation.png" /> | | <img class="" alt="Team Formation2" src="https://static.igem.org/mediawiki/2015/6/60/Team_formation.png" /> |
− | <p> Finally we formed the Team iGEM Uchile-OpenBio, we all feel part of the team and if you want to know more about the members of the team we invite you to see our <font size="42"> <a href="https://2015.igem.org/Team:UChile-OpenBio/Team" >Team Description</a></p> | + | <p> Finally we formed the Team iGEM Uchile-OpenBio, we all feel part of the team and if you want to know more about the members of the team we invite you to see our <font size="42"> <a href="https://2015.igem.org/Team:UChile-OpenBio/Team" > <img class="" alt="Team Formation1" src="https://static.igem.org/mediawiki/2015/4/4e/OB_UCHILE_BACTERIA_GUIAp1.png" /></a></p> |
| </div> | | </div> |
| </div> | | </div> |
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| <!--Team Registration--> | | <!--Team Registration--> |
| | | |
− | <div class="item_actividad"> | + | <div class="item_actividad morado"> |
| <div class="item_actividad-titulo"><span>March 2015:</span><span>Team Registration</span></div> | | <div class="item_actividad-titulo"><span>March 2015:</span><span>Team Registration</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
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| 1.- Team official clothing.<BR/> | | 1.- Team official clothing.<BR/> |
| 2.- Our official Website and social networks.<BR/> | | 2.- Our official Website and social networks.<BR/> |
− | 3.- 10 cm downside the official poster.<BR/> | + | 3.- 9 cm downside the official poster.<BR/> |
| 4.- Our Wiki project, actually this part.<BR/> | | 4.- Our Wiki project, actually this part.<BR/> |
− | Also we will honor our sponsors at the end of our presentation with special mentions. The sponsorship letter is attached at the following <a href="https://2015.igem.org/File:Uchile-openbio_OB_sponsors.pdf">link. </a></p> | + | Also we will honor our sponsors at the end of our presentation with special mentions. <a href="https://static.igem.org/mediawiki/2015/b/b0/Uchile-openbio_OB_sponsors.pdf"><img class="" alt="Team Formation1" src="https://static.igem.org/mediawiki/2015/2/24/BACTERIA_GUIA.png" /> </a></p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="item_actividad-titulo"><span>April 2015:</span><span>Team Consolidation</span></div> | | <div class="item_actividad-titulo"><span>April 2015:</span><span>Team Consolidation</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p> After the registration the team set up weekly meetings to plan the work of each week. Also each member of the team has adopted a project area. The distribution of areas was agreed by all members. <br/> | + | <p> After the registration the team set up weekly meetings to plan the work of each week. Also each member of the team adopted a project area. The distribution of areas was agreed by all members. <br/> |
− | We agreed that the responsible of each area will coordinate all the activities of that specific area. However, all team members are responsible for asisting in the areas applying for aid.<br/> | + | We agreed that the responsible of each area would coordinate all the activities of that specific area. However, all team members are responsible for asisting in the areas applying for aid.<br/> |
− | Each area has to report their advance and show their main activities for next weeks. With this assigment we are training our leadership and planning skills on each member.<br/> | + | Each area has to report their advance and show their main activities for next weeks. With this assigment we are training our leadership and planning skills on each member.</p> |
− | <b><span style="color:#CC0000"> Project areas and responsibles</span></b></p><br/> | + | <p><b><span style="color:#CC0000"> Project areas and responsibles</span></b></p> |
| <img class="" alt="Team Consolidation" src="https://static.igem.org/mediawiki/2015/a/a5/Table.png" /> | | <img class="" alt="Team Consolidation" src="https://static.igem.org/mediawiki/2015/a/a5/Table.png" /> |
| </div> | | </div> |
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| </div> | | </div> |
| | | |
− | <!-- gBlocks arrived--> | + | <!-- IDT--> |
| | | |
| | | |
| + | <div class="item_actividad morado"> |
| + | <div class="item_actividad-titulo"><span>July 2015:</span><span>Integrated DNA Technologies</span></div> |
| + | <div class="item_actividad-cuerpo"> |
| + | <p><b><span style="color:#660066">Integrated DNA Technologies</span></b> sells short strands of DNA called oligonucleotides. IDT manufactures the oligo using chemical DNA synthesis machines designed and built by them. IDT is the largest manufacturer of custom oligo products in the US today.</p> |
| + | <img class="" alt="IDT" src="https://static.igem.org/mediawiki/2015/9/94/Frontis_IDT.png" /><br> |
| + | |
| + | <p><b><span style="color:#660066"> IDT </span></b> launched a promotion applicable for all participating iGEM teams 2015. They will provde equivalent to <b><span style="color:#660066"> 20kb gBlocks Gene Fragments</span></b> from 1th april to 24 september. The avaluation of the support was around <b><span style="color:#660066">4272 USD (Golden Sponsor)</span></b>. Of course we cannot miss this chance and asked for our benefits.</p> |
| + | <img class="" alt="IDT" src="https://static.igem.org/mediawiki/2015/5/57/Collaboration_idt.jpg" /><br> |
| + | <p> <b><span style="color:#660066">Jose Duguet </span></b>contacted IDT at first with the list of g-blocks needed. They told us that they have a local distributor: Fermelo BIOTEC. <b><span style="color:#660066">Alejandro Sanchez</span></b>, technical supporter of <b><span style="color:#660066">Fermelo BIOTEC</span></b> contacted us and brought us the support , intermediating the arrival of oligonuclotides to our laboratory. We are very glad with their arrival. |
| + | <img class="" alt="IDT" src="https://static.igem.org/mediawiki/2015/6/68/Uchile-OB_FERBIOTEClogo.png" /> |
| + | </div> |
| + | </div> |
| <div class="item_actividad"> | | <div class="item_actividad"> |
− | <div class="item_actividad-titulo"><span>July 2015:</span><span>gBlocks arrived</span></div> | + | <div class="item_actividad-titulo"><span>July 2015:</span><span>Collecting Interest Letters</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p><b><span style="color:#CC0000"> gBlocks </span> </b> were provided by <span style="color:#CC0000"><b> Integrated DNA Technologies</span> </b>. The arrivals means to us that we could finally began to assemble our genetic circuit. </p>
| + | |
− | <br/> | + | <p>One of the main task given by Open Bucheff was to collect 4 interest |
− | <img class="" alt="gBlocks ARRIVED" src="https://static.igem.org/mediawiki/2015/c/c0/Collaboration_gblocks.png" /> <br>
| + | letters from different organizations. It was a necessary task if |
| + | we want to gain our Faculty support. That was how our team |
| + | members began to contact institutions that could write their letters |
| + | of interest to us. We collected a total of 6 letters of interest<br/> |
| + | <b><span style="color:#CC0000">David Sepulveda</span></b> brought the following letters<br/> |
| + | (1) Leandro Valencia, La Ruta Solar.<br/> |
| + | (2) Fernán Federici, Department of Molecular Genetics and Microbiology. |
| + | Faculty of Cs. Biological. Universidad Católica de |
| + | Chile.<br/> |
| + | Also, <b><span style="color:#CC0000">Severine Cazaux</span></b> brought 3 letters<br/> |
| + | (1) Juan Cristobal Zagal, Fablab UChile, Santiago.<br/> |
| + | (2) Claudia Tellez, Comisión Chilena de cooperación con UNESCO<br/> |
| + | (3)Andrés Briceño, Co-Director FabLab<br/> |
| + | Finally, <b><span style="color:#CC0000">Noemi Miranda</span></b> helped us with 1 letter<br/> |
| + | (1) Marcelo Mena Carrasco, Undersecretary Ministry of Environment<br/> |
| | | |
− | <p> <b><span style="color:#CC0000">But... Why are important Gblocks? </span></b> <br>
| + | Those letters realy helped us to support our project when we |
− | | + | faced our next sponsors of the project. Sponsors realy appreciate |
− | For every design in synthetic biology, a high fidelity technique is imperative to obtain large fragments of DNA for coding our gene of interest with the regulation elements. The group initially considered obtain the genetic source from the microorgansisms, amplify them and then produce the assemblies by overlap extension PCR. Thanks to gBlocks we could save all this work in laboratory that would require weeks, shipping times of biological material and we would not be able perform codon optimization </p>
| + | this cards and they get more interested on the project. </p> |
− | <img class="" alt="IDTlogo" src="https://static.igem.org/mediawiki/2015/b/ba/Idt_logo2015.jpg" />
| + | |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="item_actividad-titulo"><span>July 2015:</span><span>Open Beauchef, FCFM</span></div> | | <div class="item_actividad-titulo"><span>July 2015:</span><span>Open Beauchef, FCFM</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p><b><span style="color:#CC0000">Open Beauchef </span></b>consists of a co-working space (collaborative work) that FCFM facilities. It is located at 851 Avenue Beauchef in the OpenLab. It is designed to create a meeting place for cooperative activities that allow interaction between various innovation actors. It includes students, researchers and developers from every faculty of the Universidad de Chile, together with participants from academia, business, public sector, graduates and investors.</p> | + | <p><b><span style="color:#CC0000">OpenBeauchef </span></b>consists of a co-working space (collaborative work) that FCFM facilities. It is located at 851 Avenue Beauchef in the OpenLab. It is designed to create a meeting place for cooperative activities that allow interaction between various innovation actors. It includes students, researchers and developers from every faculty of the Universidad de Chile, together with participants from academia, business, public sector, graduates and investors.</p> |
| <img class="" alt="OpenLab FCFM0" src="https://static.igem.org/mediawiki/2015/2/25/Uchile-Openbio_OpenBeauchef.png" /> | | <img class="" alt="OpenLab FCFM0" src="https://static.igem.org/mediawiki/2015/2/25/Uchile-Openbio_OpenBeauchef.png" /> |
| | | |
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| </div> | | </div> |
| </div> | | </div> |
| + | <!-- ICEI --> |
| + | |
| | | |
− | <!-- Diario Atacama-->
| |
− | <div class="item_actividad rosado">
| |
− | <div class="item_actividad-titulo"><span>July 2015:</span><span>El Diario de Atacama</span></div>
| |
− | <div class="item_actividad-cuerpo">
| |
− | <a href="http://www.diarioatacama.cl/impresa/2015/07/28/full/cuerpo-principal/7/" target="_blank">
| |
− | <img class="" alt="El Diario de Atacama" src="https://static.igem.org/mediawiki/2015/7/7d/Collaboration_diario_atacama.jpg" />
| |
− | </a>
| |
− | </div>
| |
− | </div>
| |
| <div class="item_actividad morado"> | | <div class="item_actividad morado"> |
| <div class="item_actividad-titulo"><span>August 2015:</span><span>ICEI Ingeniería y Construcción</span></div> | | <div class="item_actividad-titulo"><span>August 2015:</span><span>ICEI Ingeniería y Construcción</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>On August 2015 ICEI Ingeniería y construcción Ltda. (Silver Sponsor) funded 1695 USD for 1 plane tickets and 1 registration fee. They also supported us with the purchase process.</p> | + | <p><b><span style="color:#660066"> ICEI Ingeniería y construcción LTDA</span></b> was funded in 1995 in Chile, as an association of professionals with proven experience in the development of industrial, mining and real estate throughout the country projects.</p> |
− | <img class="" alt="ICEI Ingeniería y Construcción Ltda." src="https://static.igem.org/mediawiki/2015/0/06/Collaboration_icei.jpg" /> | + | <img class="" alt="ICEI Ingeniería y Construcción Ltda." src="https://static.igem.org/mediawiki/2015/0/06/Collaboration_icei.jpg" /> <br/> |
| + | |
| + | <p> ICEI Ingeniería y construcción LTDA. supported us with <b><span style="color:#660066">1695 USD (Silver Sponsor)</span></b>. The money was used to pay <b><span style="color:#660066"> 1 plane tickets </span></b>and <b><span style="color:#660066">1 individual registration fee</span></b>. They also supported us with the <b><span style="color:#660066">purchase process</span></b> of 7 plane tickets by lending us money until we get the money of the Crowdfunding Campaign. |
| + | This company was contacted directly by <b><span style="color:#660066">Anariky Negrete</span></b>.<br> Finally they lend us more money, to purchase the lasts 2 plane tickets. This was a gesture of great confidence with us because we were waiting for the confirmation of next sponsor. We really appreciate what the have done for us!.</p> |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="item_actividad morado">
| + | <!-- budget--> |
− | <div class="item_actividad-titulo"><span>August 2015:</span><span>CeBiB supported us again!</span></div> | + | |
| + | <div class="item_actividad"> |
| + | <div class="item_actividad-titulo"><span>August 2015:</span><span>We have a team Budget!</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>On 13 August 2015 the team presented our iGEM competition Brochure to Juan Asenjo, director of CeBiB our actual partner. They Supported us with 5.000.000 CLP, (7309 USD at the time). This helped us to pay about 50% of the accommodation payment, 2 more plane tickets for the rest of the team and 3 inscription fee.</p> | + | <p>Our <span style="color:#CC0000">Budget</span></b> was made for 9 members of the team and then was agregated the team instructor.</p> |
| + | <img class="" alt="We have a team Budget!" src="https://static.igem.org/mediawiki/2015/d/da/Collaboration_budget.png" /> |
| + | |
| + | <p>This budget was made by <b><span style="color:#CC0000">Ignacio Ardiles</span></b>, with collaboration of <b><span style="color:#CC0000">David Sepulveda </span></b>. It definitely helped us to convince sponsors to finance the remaining costs of the team. This budget gave them clarity on the use that will be given to the funds that they will provide.</p> |
| + | |
| + | <p> After that, we realized that some items of the budget exceeded the estimated cost. As the cost of clothing and networking far exceeded our expectations. This has finally conditioned us to readjust the cost of food and transport at a much lower amount than initially estimated. Get more details of the budget attached on <a href="https://static.igem.org/mediawiki/2015/e/e7/Uchile-openbio_Team_Budget.pdf">link</a>. |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="item_actividad rosado"> | + | |
− | <div class="item_actividad-titulo"><span>August 2015:</span><span>CEBIB</span></div> | + | <!-- FCFM--> |
| + | <div class="item_actividad morado"> |
| + | <div class="item_actividad-titulo"><span>August 2015:</span><span>Support: FCFM</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <a href="http://www.cebib.cl/language/es/jovenes-investigan-como-crear-plastico-biodegradable-a-partir-de-bacterias-geneticamente-modificadas/" target="_blank">
| + | |
− | <img class="" alt="Center of Biotechnology and Bioengineering" src="https://static.igem.org/mediawiki/2015/f/f9/Collaboration_jovenes_investigan.jpg" />
| + | <p> <b><span style="color:#660066">Faculty of Physics Sciences and Mathematics (FCFM)</span></b> of the Universidad de Chile has more than 170 years of existence. It focuses on higher education and the development of basic sciences, engineering sciences and technological applications. </p> |
− | </a>
| + | <img class="" alt="FCFM" src="https://static.igem.org/mediawiki/2015/b/bd/Frontis_FCFM.png"/> <br> |
| + | <p><b><span style="color:#660066">FCFM</span></b> supported the team with 4218 USD (Golden Sponsor). The money provided is going to be used to pay the <span style="color:#660066"><B>accommodation expenses</span></b> remaining for 9 members of the team!. |
| + | <p> The contact was with <span style="color:#660066"><B>Felipe Alvarez</span></b>, (Vice-Decan of FCFM) and it was made by <span style="color:#660066"><B>Severine Cazaux</span></b> since last May!. FCFM agreed to have a meeting on August. <span style="color:#660066"><B>Luis Rodriguez</span></b>, <span style="color:#660066"><B>Severine Cazaux</span></b> and <span style="color:#660066"><B>Ignacio Ardiles</span></b> attended with a sponsor presentation, to show the current status of the project and the real potential for them: New Technology research lines around Synthetic biology.</p> |
| + | <img class="" alt="FCFM" src="https://static.igem.org/mediawiki/2015/5/53/Logo-fcfm00.png"/> <br> |
| + | </p> |
| + | <a href="https://static.igem.org/mediawiki/2015/d/d0/Uchile_OpenBio_PLA_Project.pdf"><img class="" alt="FCFM" src="https://static.igem.org/mediawiki/2015/8/87/Ob_uch_sponsor_present.png"/></a>. |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | |
| + | |
| + | <!-- FCFM--> |
| <div class="item_actividad rosado"> | | <div class="item_actividad rosado"> |
| <div class="item_actividad-titulo"><span>August 2015:</span><span>FCFM</span></div> | | <div class="item_actividad-titulo"><span>August 2015:</span><span>FCFM</span></div> |
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| <img class="" alt="FCFM" src="https://static.igem.org/mediawiki/2015/d/d6/Collaboration_fcfm.jpg" /> | | <img class="" alt="FCFM" src="https://static.igem.org/mediawiki/2015/d/d6/Collaboration_fcfm.jpg" /> |
| </a> | | </a> |
| + | <p><b><span style="color:#D91E79">Faculty of Phisics Sciences and Mathematics</span></b> published a journalistic article on its official website after their agree to be sponsors of our team. It was done by <b><span style="color:#D91E79">Noemi Miranda</span></b> (Communications officer at CeBiB) and <b><span style="color:#D91E79">Ana Maria Saez</span></b> (Communications coordinator at FCFM). </p> |
| </div> | | </div> |
| </div> | | </div> |
| + | <!-- MMA--> |
| + | |
| <div class="item_actividad rosado"> | | <div class="item_actividad rosado"> |
− | <div class="item_actividad-titulo"><span>September 2015:</span><span>El Mercurio</span></div> | + | <div class="item_actividad-titulo"><span>September 2015:</span><span>Ministry of Environment</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <a href="http://impresa.elmercurio.com/Pages/NewsDetail.aspx?dt=2015-09-07&dtB=12-09-2015%200:00:00&PaginaId=16&bodyid=1" target="_blank"> | + | <a href="http://portal.mma.gob.cl/jovenes-investigan-como-crear-plastico-biodegradable-a-partir-de-bacterias-geneticamente-modificadas/" target="_blank"> |
− | <img class="" alt="El Mercurio" src="https://static.igem.org/mediawiki/2015/a/a7/Collaboration_mercurio.jpg" /> | + | <img class="" alt="Ministerio del Medio Ambiente" src="https://static.igem.org/mediawiki/2015/2/2b/Collaboration_mmaa.jpg" /> |
| </a> | | </a> |
| + | <p>The project was highlighted on the official website of the <b><span style="color:#D91E79">Ministry of Environment</span></b> and their social networks. The ministry got interested in the project because of its sustainable character. They considered it was an environmental friendly project. The contact <b><span style="color:#D91E79">Noemi Miranda</span></b>. |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | |
| </div> | | </div> |
| + | |
| | | |
| <!--Mitad restante --> | | <!--Mitad restante --> |
| | | |
| <div class="half last"> | | <div class="half last"> |
| + | |
| + | <!-- Conception of the Idea--> |
| <div class="item_actividad"> | | <div class="item_actividad"> |
− | <div class="item_actividad-titulo"><span>January 2015:</span><span>Conception Idea</span></div> | + | <div class="item_actividad-titulo"><span>January 2015:</span><span>Conception of the Idea</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <img class="" alt="Conception Idea" src="https://static.igem.org/mediawiki/2015/3/32/Collaboration_conception_idea.png" /> | + | <p> The insight arose from the FABLAB UChile, after <b><span style="color:#CC0000">Pablo Troncoso</span></b> met <b><span style="color:#CC0000">Fernando Torres</span></b> (project engineer in FABLAB). They told us how important was the PLA in the 3D impression. We also share interest in the open source philosophy so they gave us all the support for our project including an letter of interest.</p> |
| + | <img class="" alt="Fab Lab" src="https://static.igem.org/mediawiki/2015/e/e3/Uchile_Openbio_Fab-lab.png" /> <br> |
| + | <p>After that our team looked for different uses of PLA in the society concluding that it was a powerful project and <b><span style="color:#CC0000">José Duguet</span></b> provided us the scientific literature. The conception of the idea was born and the main goal of the project is to reduce the cost of synthesis of polylactic acid to encourage a massive use of this new material. We hope to stop pollution by plastic made from fossil fuels </p> |
| + | <img class="" alt="Fab Lab" src="https://static.igem.org/mediawiki/2015/2/26/Uchile-Openbio_REACTOR.png" /> |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | <!-- New Team Support --> |
| + | |
| <div class="item_actividad morado"> | | <div class="item_actividad morado"> |
| <div class="item_actividad-titulo"><span>March 2015:</span><span>New Team Supports</span></div> | | <div class="item_actividad-titulo"><span>March 2015:</span><span>New Team Supports</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>CONICYT (Research, Science and Technology National Commission) Conicyt provided us for 4 event fee payments. We were visited this governmental organization during 4 months to look for financial support. At June we got its support. The support at this contest is around $2570 USD (Silver Sponsor).</p> | + | <p><b><span style="color:#660066">Research, Science and Technology National Commission (CONICYT)</span></b> provided us around <b><span style="color:#660066">$2570 USD (Silver Sponsor)</span></b>. We used the money to pay <b><span style="color:#660066">4 event fee payments</span></b>.<br> |
− | <img class="" alt="New Team Supports" src="https://static.igem.org/mediawiki/2015/7/7f/Collaboration_conicyt.jpg" />
| + | |
| + | <b><span style="color:#660066">CONICYT</span></b> was founded in 1967 as an advisory entity to the Presidency in matters of scientific development. Their strategic pillars are: the promotion of human capital formation and strengthening scientific and technological base of the country. </p> |
| + | <img class="" alt="New Team Supports" src="https://static.igem.org/mediawiki/2015/9/9a/Frontis_Conycit.png" /> <br> |
| + | <p> The contacts were made by<b> <span style="color:#660066">Severine Cazaux</span></b> since last may through emails to <b><span style="color:#660066">Leticia Toro</span></b>, representative of CONICYT. Our first letter sent asked for 8 inscriptions fee for the iGEM Competition.<br/> |
| + | At first we did not have answers. Then <b><span style="color:#660066">Severine Cazaux</span></b> and <b><span style="color:#660066">David Sepulveda</span></b> visited CONICYT offices requesting a response. That act was well received by the institution and finally on 18 June we got the PCI CONICYT letter, giving notice of the funds allocated for 4 people. </p> |
| + | |
| + | <img class="" alt="New Team Supports" src="https://static.igem.org/mediawiki/2015/0/04/Logo_conycit.png" /> |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | <!-- Silent Work--> |
| + | |
| <div class="item_actividad"> | | <div class="item_actividad"> |
| <div class="item_actividad-titulo"><span>May 2015:</span><span>Silent Work</span></div> | | <div class="item_actividad-titulo"><span>May 2015:</span><span>Silent Work</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
| <p><em>“Silence is the element in which all great things are formed”</em><br>Thomas Carlyle (1795-1881)</p> | | <p><em>“Silence is the element in which all great things are formed”</em><br>Thomas Carlyle (1795-1881)</p> |
| + | <p> We decided to launch a crowdfunding campaign where we could explain our project and get funds to travel to Boston, USA. The goal was to collect <b><span style="color:#CC0000">2,600,000 CLP (3715 USD)</span></b> for the next 45 days. </p> |
| <p id="video"><iframe src="https://player.vimeo.com/video/130371509?color=ffffff&title=0&byline=0&portrait=0" width="420" height="236" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <p></p></p> | | <p id="video"><iframe src="https://player.vimeo.com/video/130371509?color=ffffff&title=0&byline=0&portrait=0" width="420" height="236" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <p></p></p> |
| + | <p> The platform selected was <b><span style="color:#CC0000">Fondeadora</span></b>, a Mexican platform which has expanded its business to Chile. It has a All or nothing model. |
| + | <img class="" alt="We reach the goal!!" src="https://static.igem.org/mediawiki/2015/3/31/Collaboration_fondeadora.png" /> <br> |
| + | The campaign was designed by <b><span style="color:#CC0000">Gustavo Calvo</span></b> and <b><span style="color:#CC0000">Ignacio Ardiles</span></b>, edited by <b><span style="color:#CC0000">Natalia Urtubia</span></b> and the music of the video was provided by <b><span style="color:#CC0000">Nicolas Cazaux</span></b>, <b><span style="color:#CC0000">Severine's Brother</span></b>.<br> |
| + | The choice of platform was made for three main reasons.<br/> |
| + | 1. One of the creators of the platform came from Mexico in 2014 to present our university and we considered that it was a great and effective platform.<br/> |
| + | 2. The platform has good payment methods, such as Web-pay or transfers that are well accepted by the people of our country.<br/> |
| + | 3. The platform has offices in Chile which facilitates our direct communication.<br/> |
| + | The Campaign started on 16 of June on 2015. After that we strive to spread the campaign on our networks with different messages, banners and other kind of posts. |
| + | </p> |
| + | <img class="" alt="New Team Supports" src="https://static.igem.org/mediawiki/2015/8/8e/Banners.png"/> |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| <!-- Correccion orden linea de tiempo --> | | <!-- Correccion orden linea de tiempo --> |
| + | |
| <div class="item_actividad morado"> | | <div class="item_actividad morado"> |
| <div class="item_actividad-titulo"><span>June 2015:</span><span>SIGMA-ALDRICH</span></div> | | <div class="item_actividad-titulo"><span>June 2015:</span><span>SIGMA-ALDRICH</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>Sigma-Aldrich provided us for the laboratory reagents around USD $1900 (Golden Sponsor).<br/>
| + | <p> |
− | It was very important to decrease the time to providing reagents for project.<br/>
| + | <b><span style="color:#660066">Sigma Aldrich</span></b> is a leading Life Science and High Technology company focused on enhancing human health and safety, manufactures and distributes more than 250,000 chemicals, biochemicals and other essential products to more than 1.4 million customers globally in research and applied labs as well as in industrial and commercial markets.</p> |
− | Attach archive Sponsorship Invoice.</p>
| + | <img class="" alt="SIGMA-ALDRICH" src="https://static.igem.org/mediawiki/2015/1/12/Frontis_Sigma-Aldrich.png"/> <br> |
| + | <p><b><span style="color:#660066">Sigma-Aldrich</span></b> provided us laboratory reagents valuated around <b><span style="color:#660066"> 1900 USD (Golden Sponsor)</span></b>. The contacts were made by <b><span style="color:#660066">Luis Rodriguez</span></b>. He contacted <b><span style="color:#660066">Cristian Torres</span></b> (Company’s Sales Manager) by email, asking him for support in first time (and attaching our sponsorship letter).</p> |
| + | <p>They told us that they usually don't give funds for those instances but maybe they could support us with laboratory reactives. So after that we defined which was the list of reactives we could ask for this institution. We sent the list on may 26 and last June the reactives arrived to our laboratory. Thanks to Sigma aldrich we could start our lab experiments. |
| <img class="" alt="SIGMA-ALDRICH" src="https://static.igem.org/mediawiki/2015/f/f5/Collaboration_sigma.jpg" /> | | <img class="" alt="SIGMA-ALDRICH" src="https://static.igem.org/mediawiki/2015/f/f5/Collaboration_sigma.jpg" /> |
| + | <table style="width:100%"> |
| + | <tr> |
| + | |
| + | |
| + | |
| + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2015/6/60/Uchile-openbio_Bill_Sigma-Aldrich.pdf"><img class="" alt="New Team Supports" src="https://static.igem.org/mediawiki/2015/2/24/BACTERIA_GUIA.png" /></a></td> |
| + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2015/2/25/Uchile-openbio_Bill_Sigma-Aldrich_2.pdf"><img class="" alt="New Team Supports" src="https://static.igem.org/mediawiki/2015/a/ad/OB-Bacteria-outreach.png" /></a> |
| + | </td></tr> |
| + | </table> |
| + | |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | <!-- We reached the goal! --> |
| + | |
| + | |
| <div class="item_actividad morado"> | | <div class="item_actividad morado"> |
− | <div class="item_actividad-titulo"><span>July 2015:</span><span>We reach the goal!!</span></div> | + | <div class="item_actividad-titulo"><span>July 2015:</span><span>We reached the goal!!</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>We reached the goal of 3715 USD (Silver Sponsor). It was very important to support 4 ticket travel. We used Fondeadora crowfunding page because it is a easy donate way, we had contact direct to support and confidence in Chile, they are a rising platform in Latinoamerica. Unfortunatly, they were slow to give us the money.</p> | + | <p> Crowdfunding campaign ended succesfully on July 29th reaching a total amount of <b><span style="color:#660066">2,620,003 CLP (101% of the goal)</span></b>. The support was provided by <b><span style="color:#660066">83 differents funders </span></b>(we included the list of people how gave money in cash).</p> |
− | <img class="" alt="We reach the goal!!" src="https://static.igem.org/mediawiki/2015/3/31/Collaboration_fondeadora.png" />
| + | |
| + | <img class="" alt="CF" src="https://static.igem.org/mediawiki/2015/d/d3/Uchile_OB_ENDCAMPAIGN.png" /> |
| + | |
| + | <p>Our commitment to our funders is to provide them information about the project and then rewards as soon as we get them (It will be after the competition)</p> |
| + | |
| + | <img class="" alt="CF" src="https://static.igem.org/mediawiki/2015/c/c7/Uchile-openbio-Crowd-funders.png" /><br> |
| + | |
| + | <p> Finally about the money withdraw from Fondeadora, we consider that it was a tedious process. That is because they told us we could withdraw the money 5 days after reaching the goal. But the days passed and the money did not arrive until 31 August where we finally got our <b><span style="color:#660066">3378 USD </span></b> (The fee was around 12,3% of total).</p> |
| + | |
| + | <p>Finally, they told us the origin of the problem (Bank issues). Despite this, we still consider Fondeadora is a good platform for funding your projects, but you may talk about the money arrival time. </p> |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="item_actividad morado">
| + | <!-- IDT--> |
− | <div class="item_actividad-titulo"><span>July 2015:</span><span>IDT</span></div> | + | |
| + | <div class="item_actividad"> |
| + | <div class="item_actividad-titulo"><span>July 2015:</span><span>gBlocks arrived</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>We use the support of IDT(Golden Sponsor) to the iGEM’s teams for synthesize the biobricks that we used in our proyect. This biobricks came in the format of gBlocks.</p> | + | <p><b><span style="color:#CC0000"> gBlocks </span> </b> were provided by <span style="color:#CC0000"><b> Integrated DNA Technologies</span> </b>. The arrival meant to us that we could finally began to assemble our genetic circuits. </p> |
− | <img class="" alt="IDT" src="https://static.igem.org/mediawiki/2015/5/57/Collaboration_idt.jpg" />
| + | <br/> |
| + | <img class="" alt="gBlocks ARRIVED" src="https://static.igem.org/mediawiki/2015/c/c0/Collaboration_gblocks.png" /> <br> |
| + | |
| + | <p> <b><span style="color:#CC0000">But... Why are gBlocs so important? </span></b> <br> |
| + | |
| + | For every design in synthetic biology, a high fidelity technique is imperative to obtain large fragments of DNA for coding our gene of interest with the regulation elements. The team initially considered to obtain the genetic source from microorgansisms, amplify them and then produce the assemblies by overlap extension by PCR. Thanks to gBlocks we could save all this work in laboratory that would require weeks, shipping times of biological material and we would not have been able perform codon optimization </p> |
| + | <img class="" alt="IDTlogo" src="https://static.igem.org/mediawiki/2015/b/ba/Idt_logo2015.jpg" /> |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="item_actividad"> | + | |
− | <div class="item_actividad-titulo"><span>July 2015:</span><span>Collecting Interest Letters</span></div> | + | |
| + | |
| + | <!---- Budget--> |
| + | <div class="item_actividad rosado"> |
| + | <div class="item_actividad-titulo"><span>July 2015:</span><span>El Diario de Atacama</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | **** COMPLETAR | + | <a href="http://www.diarioatacama.cl/impresa/2015/07/28/full/cuerpo-principal/7/" target="_blank"> |
| + | <img class="" alt="El Diario de Atacama" src="https://static.igem.org/mediawiki/2015/7/7d/Collaboration_diario_atacama.jpg" /> |
| + | </a> |
| + | <p> <b><span style="color:#D91E79">Diario Atacama</span></b> is a daily newspaper of the Atacama Region, Chile. The contact done was with <b><span style="color:#D91E79">Catalina Canales</span></b> (Journalist at Diario Atacama) and it was done by <b><span style="color:#D91E79">Ignacio Ardiles</span></b>. Also it was possible thanks to the project description done by <b><span style="color:#D91E79">David Sepulveda</span></b>. The project description is attached at the following <a href="https://static.igem.org/mediawiki/2015/a/a2/Ficha_Plastico_Biodegradable_UChile_OpenBio.docx.pdf"><img class="" alt="El Diario de Atacama" src="https://static.igem.org/mediawiki/2015/b/b4/OB-BACT-OUTREACH.png" /></a>.</p> |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="item_actividad"> | + | |
− | <div class="item_actividad-titulo"><span>August 2015:</span><span>We have a team Budget!</span></div> | + | |
| + | |
| + | <!-- Mariano--> |
| + | <div class="item_actividad rosado"> |
| + | <div class="item_actividad-titulo"><span>August 2015:</span><span>Mariano School</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>Finaly on 10 August we made our budget and we were ready to present it to interested Organizations. At that date we had covered 43% of the total expenses.</p> | + | <a href="http://www.colegiomariano.cl/sitio/index.php/servicios/noticias-nulo/noticias-de-colegio/694-la-biologia-desde-una-nueva-perspectiva" target="_blank"> |
− | <img class="" alt="We have a team Budget!" src="https://static.igem.org/mediawiki/2015/d/da/Collaboration_budget.png" />
| + | <img class="" alt="Colegio Mariano" src="https://static.igem.org/mediawiki/2015/f/f9/Collaboration_mariano.jpg" /> |
| + | </a> |
| + | <p><b><span style="color:#D91E79">Mariano School</span></b> is a local school where did our Human Practice workshop. The activities where commanded by <b><span style="color:#D91E79">Severine Cazux</span></b>. For this occasion she was accompanied by <b><span style="color:#D91E79">Luis Rodriguez</span></b> and <b><span style="color:#D91E79">Hector Ampuero</span></b>.</p> |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="item_actividad"> | + | |
− | <div class="item_actividad-titulo"><span>August 2015:</span><span>Support: FCFM</span></div> | + | |
| + | |
| + | <!-- CeBiB again--> |
| + | |
| + | |
| + | <div class="item_actividad morado"> |
| + | <div class="item_actividad-titulo"><span>August 2015:</span><span>CeBiB supported us again!</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | **** COMPLETAR | + | <p><b><span style="color:#660066">CeBiB</span></b> supported us again with <b><span style="color:#660066"> 7309 USD</span></b> (they became our <span style="color:#660066">Platinum Partner</span></b>). The money was used to pay the <b><span style="color:#660066">reservation on the 40 Berkeley Hostal, 2 plane tickets, 3 individual inscription fee, merchandising, the Official Clothing and other travel expenses.</span></b></p> |
| + | <img class="" alt="Cebib" src="https://static.igem.org/mediawiki/2015/f/f4/Team_CeBiB.png" /> <br> |
| + | <p>The colaboration of CeBiB was announced after <b><span style="color:#660066">Luis Rodriguez</span></b> and <b><span style="color:#660066">Ignacio Ardiles</span></b> had a meeting with our instructors <b><span style="color:#660066">Dr Juan Asenjo</span></b> and <b><span style="color:#660066">Alvaro Olivera</span></b>. We presented our Team planning <b><span style="color:#660066">brochure</span></b> with main activities and objectives of our visit tour at Boston USA. We also presented the team Budget and the letters of interest collected for OpenBeauchef. <a href="https://static.igem.org/mediawiki/2015/0/0a/Uchile-openbio_USA_Embassy.pdf"><img class="" alt="Cebib" src="https://static.igem.org/mediawiki/2015/6/6f/OB_UC_BROCHURE.png" /></a> |
| + | </p> |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | |
| + | <!-- CeBiB--> |
| + | |
| <div class="item_actividad rosado"> | | <div class="item_actividad rosado"> |
− | <div class="item_actividad-titulo"><span>August 2015:</span><span>Colegio Mariano</span></div> | + | <div class="item_actividad-titulo"><span>August 2015:</span><span>CEBIB</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <a href="http://www.colegiomariano.cl/sitio/index.php/servicios/noticias-nulo/noticias-de-colegio/694-la-biologia-desde-una-nueva-perspectiva" target="_blank"> | + | <a href="http://www.cebib.cl/language/es/jovenes-investigan-como-crear-plastico-biodegradable-a-partir-de-bacterias-geneticamente-modificadas/" target="_blank"> |
− | <img class="" alt="Colegio Mariano" src="https://static.igem.org/mediawiki/2015/f/f9/Collaboration_mariano.jpg" /> | + | <img class="" alt="Center of Biotechnology and Bioengineering" src="https://static.igem.org/mediawiki/2015/f/f9/Collaboration_jovenes_investigan.jpg" /> |
| </a> | | </a> |
| + | <p> <b><span style="color:#D91E79">CeBiB</span></b> published a journalistic article on its official website after their huge resources support. It was done by <b><span style="color:#D91E79">Noemi Miranda</span></b> (Communications Officer at CeBiB). </p> |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | |
| + | <!--CLM--> |
| <div class="item_actividad rosado"> | | <div class="item_actividad rosado"> |
− | <div class="item_actividad-titulo"><span>September 2015:</span><span>Ministerio del Medio Ambiente</span></div> | + | <div class="item_actividad-titulo"><span>September 2015:</span><span>La Maisonnette School</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <a href="http://portal.mma.gob.cl/jovenes-investigan-como-crear-plastico-biodegradable-a-partir-de-bacterias-geneticamente-modificadas/" target="_blank"> | + | <a href="http://www.lamaisonnette.cl/noticias.php?id=2915" target="_blank"> |
− | <img class="" alt="Ministerio del Medio Ambiente" src="https://static.igem.org/mediawiki/2015/2/2b/Collaboration_mmaa.jpg" /> | + | <img class="" alt="Colegio La Maisonnette" src="https://static.igem.org/mediawiki/2015/0/0a/Collaboration_maisonnette.jpg" /> |
| </a> | | </a> |
| + | <p><b><span style="color:#D91E79">La Maissonnette School</span></b> is a local school where the group did another Human Practice workshop. The activities where commanded by <b><span style="color:#D91E79">Severine Cazux</span></b>. For this occasion we attended on 2 different times to the school. At first <b><span style="color:#D91E79">Javiera Gomez</span></b> and <b><span style="color:#D91E79">Gustavo Calvo</span></b> accompanied Severine. For the second time <b><span style="color:#D91E79">Hector Ampuero</span></b> and <b><span style="color:#D91E79">Javiera Gomez</span></b> accompanied her.</p> |
| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | <!-- Mercurio--> |
| + | |
| <div class="item_actividad rosado"> | | <div class="item_actividad rosado"> |
− | <div class="item_actividad-titulo"><span>September 2015:</span><span>Colegio La Maisonnette</span></div> | + | <div class="item_actividad-titulo"><span>September 2015:</span><span>El Mercurio</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <a href="http://www.lamaisonnette.cl/noticias.php?id=2915" target="_blank"> | + | <a href="http://impresa.elmercurio.com/Pages/NewsDetail.aspx?dt=2015-09-07&dtB=12-09-2015%200:00:00&PaginaId=16&bodyid=1" target="_blank"> |
− | <img class="" alt="Colegio La Maisonnette" src="https://static.igem.org/mediawiki/2015/0/0a/Collaboration_maisonnette.jpg" /> | + | <img class="" alt="El Mercurio" src="https://static.igem.org/mediawiki/2015/a/a7/Collaboration_mercurio.jpg" /> |
| </a> | | </a> |
| + | <p><b><span style="color:#D91E79">El Mercurio</span></b> is one of the most recognized newspapper in Chile with large national outreach. The contact was done by <b><span style="color:#D91E79">Noemi Miranda</span></b> (Communications officer at CeBiB) and <b><span style="color:#D91E79">Ana Maria Saez</span></b> (Communications coordinator at FCFM). </p> |
| </div> | | </div> |
| </div> | | </div> |
| </div> | | </div> |
| </div> | | </div> |
| + | <img class="" alt="El Mercurio" src="https://static.igem.org/mediawiki/2015/3/35/GOAL.png" /> |
| + | <img class="" alt="El Mercurio" src="https://static.igem.org/mediawiki/2015/d/d4/Bacteriasssssss.png" /><br> |
| + | <p> <b> Thank You all for your support! We really appreciate what have you done for us!</b> <br> |
| + | <b> Also we want to thanks to:</b> Carlos Gómez - María Isabel Guerra - Irene Román - Nicolás Salas - Rodrigo Valenzuela - Amelia Cox Fermandois - Tatiana Jimenez - Pablo Alvarez - Rosario Rosales - Lucas Sepúlveda - Humberto Palza - Hector Concha - Nancy Carrasco - Paz Zañartu - Ana María Saez - Pablo Troncoso - Ericka Jorquera - Heinz Gerdin </p> |
| + | |
| </article> | | </article> |
| + | |
| </section> | | </section> |
| <script> | | <script> |