Difference between revisions of "Team:Tuebingen/Attributions"

 
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<h2> Project Description</h2>
 
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<h4> A Biosensor Memory Module: Cre Sensor</h4>
 
  
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<p>Analysis and imaging methods are an indispensable element of every scientific study - in a wider sense the results are only as good as their measurement methods.<br/>
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Photostimulation is one of the most important non-invasive analysis methods allowing researchers to examine the relationship between metabolic processes, e.g. through activating a molecule via light treatment. We want to create a module which makes it possible to take a snapshot of the activity of a sensor at any time.
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dias = ['General Support','Acknowledgement','Financial Sponsors','Lab Sponsors'];
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Our designed system should be capable of capturing such a ‘snapshot’ of the activity of a sensor. Therefore our aim is to create a Cre recombinase whose activity can be reversibly controlled by light. By activating this construct only for a short period of time, we can use a Cre to switch on expression of Luciferase in only a limited amount of the sensor cells. Through coupling the Cre expression to the sensor we can thereby permanently write the sensor state of a given time point into the DNA of a system. </p>
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To achieve the construction of a reversibly activatable Cre recombinase we want to apply the caging mechanism described by Zhou et al [1]. This caging is performed by fusing a copy of a variant of the fluorescent protein Dronpa to both the C- and N-terminus of the Cre recombinase. Since this Dronpa variant is able to form monomers or dimers depending on illumination with light of different wavelengths, we hope that the dimerized form inhibits the activity of the Cre recombinase. </p>
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<p>Thank you to everyone who helped us and Cellfie to our goal!</b> </p>
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<p>
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<p>Thanks to Prof. Dr. Alfred Nordheim of the Interfaculty Institute for Cell Biology, Prof. Dr. Oliver Kohlbacher of the Center for Bioinformatics and Prof. Dr. Ralf-Peter Jansen of the Interfaculty Institute for Biochemistry at the University Tuebingen for making the participation in iGEM possible and giving us advice on our project idea.</p>
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Because our system only needs the expression of the caged Cre construct to be dependent on a sensor, it can be combined with almost all Biosensors that include a means of transcriptional control. This gives the system a wide variety of possible applications, especially in the context of the work of other iGEM teams.</p>
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<p> There were many other people and research groups that helped us, so we also thank</p>
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<ul>
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<li> AG Feil (special thanks to Barbara) for everything</li>
 +
<li> AG Jansen for their yeast</li>
 +
<li> AG García-Sáez, especially Aida, for microscopy</li>
 +
<li> AG Schwarzer for lab materials</li>
 +
<li> PD Dr. Elisabeth Fuss for materials and several other things</li>
 +
<li> PD Dr. Frank Essmann from AG Schulze-Osthoff for primer and gel documentation</li>
 +
<li> Dr. Christoph Schall from AG Stehle </li>
 +
<li> Prof. Dr. Ulrich Rothbauer for the GFP-trap</li>
 +
<li> Prof. Dr. Ulrike Zentgraf for lab materials</li>
 +
<li> Prof. Dr. Frank Edenhofer for the use of his cell-penetrating Cre recombinase</li>
 +
<li> Dr. Kai Braun from AG Meixner for providing equipment and expertise to build the monochromator device</li>
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</ul>
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<br/>
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</div>
  
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<p>[1] Zhou, XX; Chung, HK; Lam, AJ & Lin, MZ. (2012). Optical control of protein activity by fluorescent protein domains. Science, 338(6108), 810-814.</p>
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<img id="teamfoto" style="width:100%;height:auto;float:center;" src="https://static.igem.org/mediawiki/2015/c/c7/TeamTuebingen_TeamPictureClose.jpg"/>
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<h4>Organisation</h4>
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<p> Daniela Nguyen, Nikolas Layer, Philipp Mayer did our general organisation, from organizing weekly meetings to coordination groups to communicating with the university and other institutions. </p>
  
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<h4>Project idea</h4>
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<p>Even though the whole team was involved in the development of the project idea, special thanks to Katharina Sporbeck and Nicolai von Kuegelgen of the team for developing our project idea with special efforts.</p>
 +
 +
<h4>Lab work and summercamp</h4>
 +
<p>Without our lab team, Kevin Sabath, Katharina Sporbeck, Nicolai von Kuegelgen and Lisa Wellinger we would not have the results we have and also our summercamp would have not existed - from organizing the camp to instructing and showing the bachelor students how to work in a lab everything was done by them. </p>
 +
 +
<h4>Fundraising</h4>
 +
<p>Nicolas Layer, Daniela Nguyen, Edwin Schmierer (GoCoTe e.V.) were responsible for fundraising, prepared and wrote e-mails to many companies as well as organizing "Kleinkampftage".</p>
 +
<div style="width:1005;text-align:center;"><img id="gocote" style="max-width:300px; display: center; margin-left: auto; margin-right: auto;" src="https://static.igem.org/mediawiki/2015/a/aa/Team_Tuebingen_gocote_logo.png"/></div>
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<h4 style="clear:both;">Wiki and homepage</h4>
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<p>Tim Kucera and Daniela Nguyen build wiki and homepage and fed them with contents.</p>
 +
 +
<h4>Design and drawings</h4>
 +
<p>In times of waiting and boredom we had so much fun because of Cellfie and his colleagues. Without Katerina Romanova and Yvonne Thoma Cellfie and his Cellvengers would not exist as well as our wiki graphics.</p>
 +
 +
<h4>Human Practices</h4>
 +
<p>Jens Reich organized our SynBio Day in the historic center of Tuebingen and with Sarah Roffeis the participation in a scientific speed dating in Freiburg. Also Sarah managed the programm during our summercamp and saw to it that we had a lot of fun. They both put much effort in inviting a school class to our lab and delivering them an insight into the world of synthetic biology. In addition they organized our School class@lab project day together with Vivien Weber and Philipp Mayer who communicated with the iGEM Team and the school class teacher.</p>
 +
 +
<h4>Modeling</h4>
 +
<p>Adrian Geissler wrote and implemented all our modeling codes and helped with general data analysis.</p>
 +
 +
<h4>Cooperation</h4>
 +
<p>Vivien Weber communicated with our cooperation partners.</p>
 +
 +
</div>
 +
 +
<div id="dia2" class="dia">
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<p>We definitely want to mention those who did us an incredible favour by supporting us financially - without you we would not have been able to do any of the work we did:</p>
 +
<ul>
 +
<li>Deanery of the Mathematics and Science Faculty of the University Tuebingen</li>
 +
<li>Prorektor Forschung of the University Tuebingen</li>
 +
<li>Interfaculty Institute of Biochemistry, University Tuebingen</li>
 +
<li>SFB685 Immunology, University Tuebingen</li>
 +
<li>PROMOS foundation</li>
 +
<li> Boehringer Ingelheim </li>
 +
<li>Kreissparkasse Tuebingen</li>
 +
</ul>
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<div style="line-height:100px;width:300px;background-color:white;display:inline-block;height:100px;"><img style="vertical-align:middle;max-width:100%;max-height:100%;" id="boehringeringelheim" src="https://static.igem.org/mediawiki/2015/2/21/Team_Tuebingen_Logo_KSK_weiss_auf_rot.jpg" /></div>
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<p> Same goes for those who supported us with lab materials - thank you very much!</p>
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<ul>
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<li>Agilent</li>
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<li>Biozym</li>
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<li>GATC</li>
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<li>Greiner</li>
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<li>Labfolder</li>
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<li>Promega Corporation</li>
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<li>Serva</li>
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<li>Snapgene</li>
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</ul>
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<div style="margin:2px;line-height:100px;width:300px;background-color:white;display:inline-block;height:100px;"><img id="agilent" style="vertical-align:middle;max-width:100%;max-height:100%;"  src="https://static.igem.org/mediawiki/2015/9/91/Team_Tuebingen_Biozym.jpg" /></div>
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<div style="margin:2px;line-height:100px;width:300px;background-color:white;display:inline-block;height:100px;"><img id="agilent" style="vertical-align:middle;max-width:100%;max-height:100%;"  src="https://static.igem.org/mediawiki/2015/c/cb/Team_Tuebingen_Logo_gatc_new.jpg" /></div>
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<div style="margin:2px;line-height:100px;width:300px;background-color:white;display:inline-block;height:100px;"><img id="agilent" style="vertical-align:middle;max-width:100%;max-height:100%;"  src="https://static.igem.org/mediawiki/2015/e/e2/Team_Tuebingen_greiner_Logo.jpg" /></div>
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<div style="margin:2px;line-height:100px;width:300px;background-color:white;display:inline-block;height:100px;"><img id="agilent" style="vertical-align:middle;max-width:100%;max-height:100%;"  src="https://static.igem.org/mediawiki/2015/5/57/Team_Tuebingen_labfolder_logo_01_tagline_2250x500.png" /></div>
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<div style="margin:2px;line-height:100px;width:300px;background-color:white;display:inline-block;height:100px;"><img id="agilent" style="vertical-align:middle;max-width:100%;max-height:100%;"  src="https://static.igem.org/mediawiki/2015/6/6e/Team_Tuebingen_SERVA_logo.jpg" /></div>
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<div style="margin:2px;line-height:100px;width:300px;background-color:white;display:inline-block;height:100px;"><img id="agilent" style="vertical-align:middle;max-width:100%;max-height:100%;"  src="https://static.igem.org/mediawiki/2015/2/29/Team_Tuebingen_Sponsors_SnapGene.png" /></div>
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<div style="margin:2px;line-height:100px;width:300px;background-color:white;display:inline-block;height:100px;"><img id="agilent" style="vertical-align:middle;max-width:100%;max-height:100%;"  src="https://static.igem.org/mediawiki/2015/b/b1/Team_Tuebingen_Logo_Promega_SOL.JPG" /></div>
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Latest revision as of 12:16, 17 November 2015

<
>

Thank you to everyone who helped us and Cellfie to our goal!

Thanks to Prof. Dr. Alfred Nordheim of the Interfaculty Institute for Cell Biology, Prof. Dr. Oliver Kohlbacher of the Center for Bioinformatics and Prof. Dr. Ralf-Peter Jansen of the Interfaculty Institute for Biochemistry at the University Tuebingen for making the participation in iGEM possible and giving us advice on our project idea.

There were many other people and research groups that helped us, so we also thank

  • AG Feil (special thanks to Barbara) for everything
  • AG Jansen for their yeast
  • AG García-Sáez, especially Aida, for microscopy
  • AG Schwarzer for lab materials
  • PD Dr. Elisabeth Fuss for materials and several other things
  • PD Dr. Frank Essmann from AG Schulze-Osthoff for primer and gel documentation
  • Dr. Christoph Schall from AG Stehle
  • Prof. Dr. Ulrich Rothbauer for the GFP-trap
  • Prof. Dr. Ulrike Zentgraf for lab materials
  • Prof. Dr. Frank Edenhofer for the use of his cell-penetrating Cre recombinase
  • Dr. Kai Braun from AG Meixner for providing equipment and expertise to build the monochromator device

Organisation

Daniela Nguyen, Nikolas Layer, Philipp Mayer did our general organisation, from organizing weekly meetings to coordination groups to communicating with the university and other institutions.

Project idea

Even though the whole team was involved in the development of the project idea, special thanks to Katharina Sporbeck and Nicolai von Kuegelgen of the team for developing our project idea with special efforts.

Lab work and summercamp

Without our lab team, Kevin Sabath, Katharina Sporbeck, Nicolai von Kuegelgen and Lisa Wellinger we would not have the results we have and also our summercamp would have not existed - from organizing the camp to instructing and showing the bachelor students how to work in a lab everything was done by them.

Fundraising

Nicolas Layer, Daniela Nguyen, Edwin Schmierer (GoCoTe e.V.) were responsible for fundraising, prepared and wrote e-mails to many companies as well as organizing "Kleinkampftage".

Wiki and homepage

Tim Kucera and Daniela Nguyen build wiki and homepage and fed them with contents.

Design and drawings

In times of waiting and boredom we had so much fun because of Cellfie and his colleagues. Without Katerina Romanova and Yvonne Thoma Cellfie and his Cellvengers would not exist as well as our wiki graphics.

Human Practices

Jens Reich organized our SynBio Day in the historic center of Tuebingen and with Sarah Roffeis the participation in a scientific speed dating in Freiburg. Also Sarah managed the programm during our summercamp and saw to it that we had a lot of fun. They both put much effort in inviting a school class to our lab and delivering them an insight into the world of synthetic biology. In addition they organized our School class@lab project day together with Vivien Weber and Philipp Mayer who communicated with the iGEM Team and the school class teacher.

Modeling

Adrian Geissler wrote and implemented all our modeling codes and helped with general data analysis.

Cooperation

Vivien Weber communicated with our cooperation partners.

We definitely want to mention those who did us an incredible favour by supporting us financially - without you we would not have been able to do any of the work we did:

  • Deanery of the Mathematics and Science Faculty of the University Tuebingen
  • Prorektor Forschung of the University Tuebingen
  • Interfaculty Institute of Biochemistry, University Tuebingen
  • SFB685 Immunology, University Tuebingen
  • PROMOS foundation
  • Boehringer Ingelheim
  • Kreissparkasse Tuebingen

Same goes for those who supported us with lab materials - thank you very much!

  • Agilent
  • Biozym
  • GATC
  • Greiner
  • Labfolder
  • Promega Corporation
  • Serva
  • Snapgene