Difference between revisions of "Team:Peking/Basic Part"

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<html lang="en">
 
<head>
 
<head>
 
   <meta charset="utf-8">
 
   <meta charset="utf-8">
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   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Blue/new?action=raw&amp;ctype=text/css " title="blue" media="screen" />
 
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Blue/new?action=raw&amp;ctype=text/css " title="blue" media="screen" />
 
   <style type="text/css">/*get rid of the annoying iGEM style*/
 
   <style type="text/css">/*get rid of the annoying iGEM style*/
−
        #top_menu_inside, #top_menu_inside:before, #top_menu_inside:after { -webkit-box-sizing: content-box; -moz-box-sizing: border-box; box-sizing: content-box;}  
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#top_menu_inside, #top_menu_inside:before, #top_menu_inside:after { -webkit-box-sizing: content-box; -moz-box-sizing: border-box; box-sizing: content-box;}  
 
         #top_menu_under{font-size: 12px;}
 
         #top_menu_under{font-size: 12px;}
 
         #top_menu_14{font-size: 12px; height:18px}
 
         #top_menu_14{font-size: 12px; height:18px}
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   background-color:#e0e0e0;margin-top:1%;margin-bottom:20px;padding:20px;padding-top:40px;
 
   background-color:#e0e0e0;margin-top:1%;margin-bottom:20px;padding:20px;padding-top:40px;
 
}
 
}
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body{
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  text-align:justify;
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}
 
     </style>
 
     </style>
 
</head>
 
</head>
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     <!-- Start Header -->
 
     <!-- Start Header -->
 
     <header class="clearfix">
 
     <header class="clearfix">
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         <!-- Start  Logo &amp; Naviagtion  -->
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         <!-- Start  Logo & Naviagtion  -->
 
             <div class="navbar navbar-default navbar-top">
 
             <div class="navbar navbar-default navbar-top">
 
                 <div class="container">
 
                 <div class="container">
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                     <div class="navbar-collapse collapse"  style="padding-top:5px;padding-bottom:0">
 
                     <div class="navbar-collapse collapse"  style="padding-top:5px;padding-bottom:0">
 
                         <!-- Start Navigation List -->
 
                         <!-- Start Navigation List -->
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                         <ul class="nav navbar-nav navbar-right " style="padding-bottom:15px;height:88px;padding-top:10px">
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                         <ul class="nav navbar-nav navbar-right " style="padding-bottom:15px;padding-top:10px">
 
                             <li>
 
                             <li>
 
                                 <a href="https://2015.igem.org/Team:Peking/JudgingCriteria" class="active">Achievements</a>
 
                                 <a href="https://2015.igem.org/Team:Peking/JudgingCriteria" class="active">Achievements</a>
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                 </div>
 
                 </div>
 
             </div>
 
             </div>
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             <!-- End Header Logo &amp; Naviagtion -->
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             <!-- End Header Logo & Naviagtion -->
 
              
 
              
 
         </header>  
 
         </header>  
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         <div class="row">
 
         <div class="row">
 
           <div class="col-md-6">
 
           <div class="col-md-6">
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             <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >P<span style="text-transform:lowercase">arts</span></h2>
+
             <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >Parts</h2>
 
             <p style="margin-top:0px;font-size:14px" id="Overview">Each part give us a new experience.</p>
 
             <p style="margin-top:0px;font-size:14px" id="Overview">Each part give us a new experience.</p>
 
           </div>
 
           </div>
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                 <ul>
 
                 <ul>
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Parts">Parts Document</a>
 
                 </li>
 
                 </li>
 
                  
 
                  
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection" style="color:#00afd1">Parts Collection</a>
 
                 </li>
 
                 </li>
 
                
 
                
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                 <ul>
 
                 <ul>
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Parts">Parts Document</a>
 
                 </li>
 
                 </li>
 
                  
 
                  
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Part_Collection" style="color:#00afd1">Parts Collection</a>
 
                 </li>
 
                 </li>
 
                
 
                
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           <!-- Page Content -->
 
           <!-- Page Content -->
−
           <div class="col-md-9 page-content" style="text-align:justify;">
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           <div class="col-md-9 page-content">
 
             <div id="Part-Favorite part" id="Document">
 
             <div id="Part-Favorite part" id="Document">
 
             <!-- Classic Heading -->
 
             <!-- Classic Heading -->
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                 <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3>
 
                 <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3>
−
<!--<div>
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<div style="margin-bottom:10px;">
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<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>-->
+
<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>
−
<div class="col-md-12">
+
<!--<div class="col-md-12">
 
   <table class="table table-hover table-striped" style="color:#666;font-size:14px">  
 
   <table class="table table-hover table-striped" style="color:#666;font-size:14px">  
 
<thead>
 
<thead>
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<tbody>
 
<tbody>
 
<tr>  
 
<tr>  
−
   <td>&#160;&#8730;</td>
+
   <td>&nbsp;&radic;</td>
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">BBa_K1689000</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">BBa_K1689000</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
 
<td>sgRNA generator</td>  
 
<td>sgRNA generator</td>  
−
<td>ZHAO Xuejin</td>  
+
<td>WEI Weijia</td>  
 
</tr>  
 
</tr>  
  
 
<tr>  
 
<tr>  
−
   <td>&#160;&#8730;</td>
+
   <td>&nbsp;&radic;</td>
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
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<tr>  
 
<tr>  
−
   <td>&#160;&#8730;</td>
+
   <td>&nbsp;&radic;</td>
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>  
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<td>&nbsp;</td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>  
 
<td>Compostie</td>  
 
<td>Compostie</td>  
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<tr>  
 
<tr>  
−
   <td>&#160;</td>  
+
   <td>&nbsp;</td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">BBa_K1689001</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">BBa_K1689001</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
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<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">BBa_K1689002</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">BBa_K1689002</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>  
 
<tr>  
−
   <td>&#160;</td>  
+
   <td>&nbsp;</td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">BBa_K1689003</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">BBa_K1689003</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>  
 
<tr>  
−
   <td>&#160;</td>  
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   <td>&nbsp;</td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1">BBa_K1689004</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1">BBa_K1689004</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>  
 
<tr>  
−
<td>&#160;</td>  
+
<td>&nbsp;</td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">BBa_K1689005</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">BBa_K1689005</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>  
 
<tr>  
−
   <td>&#160;</td>  
+
   <td>&nbsp;</td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1">BBa_K1689006</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1">BBa_K1689006</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>  
 
<td>Composite</td>  
 
<td>Composite</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>  
 
<td>Compostie</td>  
 
<td>Compostie</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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<tr>
 
<tr>
−
<td>&#160;</td>   
+
<td>&nbsp;</td>   
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>  
 
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>  
 
<td>Coding</td>  
 
<td>Coding</td>  
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</tr>  
 
</tr>  
 
</table>  
 
</table>  
−
</div>
+
</div>-->
  
 
                 <p><b>Parts for paired dCas9 reporter system</b></p>
 
                 <p><b>Parts for paired dCas9 reporter system</b></p>
  
−
                 <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>,<br/> FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>,<br/> Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a>, <br/>FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a><br/> are used to validate the reconsitituation activity of split luciferase.</p>
+
                 <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>, FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>, Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a> and FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a> are used to validate the reconsitituation activity of split luciferase.</p>
−
                 <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>,<br/> Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>,<br/> dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a>,<br/> dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a><br/> fusion proteins are used for constructing paired dCas9 reporter system.</p>
+
                 <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>, Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>, dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a> and dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a> fusion proteins are used for constructing paired dCas9 reporter system.</p>
−
                 <p>&#916;α-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>,<br/> dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>,<br/> Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>,<br/> dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>,<br/> F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>,<br/> dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, <br/>F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>,<br/> dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>,<br/> dCas9-&#916;&#945;<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>,<br/> &#916;&#969;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a><br/> are used for further development of dectection.</p>
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                 <p>&Delta;α-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>, dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>, Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>, dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>, F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>, dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>,  dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>, dCas9-&Delta;&alpha;<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>, &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a> are used for further development of dectection.</p>
 
                 <p><b>Parts for Molecular Beacon</b></p>
 
                 <p><b>Parts for Molecular Beacon</b></p>
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                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
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                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a> and Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a> are used for binding to molecular beacon.</p>
 
</div>
 
</div>
 
                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
 
                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
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                 <p><b>dCas9-splitβ–Galactosidase Parts</b></p>
 
                 <p><b>dCas9-splitβ–Galactosidase Parts</b></p>
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                 <p>&#916;&#945;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">(BBa_K1689011)</a><br/>
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                 <p>&Delta;&alpha;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">(BBa_K1689011)</a><br/>
−
                 dCas9-&#916;&#945; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">(BBa_K1689019)</a><br/>
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                 dCas9-&Delta;&alpha; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">(BBa_K1689019)</a><br/>
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                 &#916;&#969;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a></p>
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                 &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a></p>
 
                 <p>Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.</p>
 
                 <p>Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.</p>
 
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                             <p class="text-center">&#169; 2015 Peking iGEM -  All Rights Reserved. Based on <a href="http://getbootstrap.com/"style="color:#b4b4b4">Bootstrap</a> and Margo template.</p>
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                             <p class="text-center">&copy; 2015 Peking iGEM -  All Rights Reserved. Based on <a href="http://getbootstrap.com/"style="color:#b4b4b4">Bootstrap</a> and Margo template.</p>
 
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Revision as of 13:05, 17 November 2015

Peking iGEM 2015

Parts

Each part give us a new experience.

Favorite Parts

Nluc-dCas9 fusion protein
(BBa_K1689010)

Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein (BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.

Cluc-dCas9 fusion protein
(BBa_K1689009)

Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein (BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.

sgRNA generator
(BBa_K1689000)

sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.

Part List

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Parts for paired dCas9 reporter system

Nluc416-FRB (BBa_K1689003), FKBP-Cluc398 (BBa_K1689005), Nluc398-FRB (BBa_K1689004) and FKBP-Cluc394 (BBa_K1689006) are used to validate the reconsitituation activity of split luciferase.

Nluc-dCas9(BBa_K1689010), Cluc-dCas9(BBa_K1689009), dCas9-Nluc (BBa_K1689008) and dCas9-Cluc (BBa_K1689007) fusion proteins are used for constructing paired dCas9 reporter system.

Δα-dCas9 (BBa_K1689011), dCas9-Nlact (BBa_K1689012), Nlact-dCas9 (BBa_K1689013), dCas9-Clact (BBa_K1689014), F[1,2]-dCas9 (BBa_K1689015), dCas9-F[1,2] (BBa_K1689016), F3-dCas9 (BBa_K1689017), dCas9-F3 (BBa_K1689018), dCas9-Δα (BBa_K1689019), Δω-dCas9 (BBa_K1689020) are used for further development of dectection.

Parts for Molecular Beacon

STV-Nluc (BBa_K1689001) and Cluc-STV (BBa_K1689002) are used for binding to molecular beacon.

Parts for sgRNA generation

sgRNA generator (BBa_K1689000) is used for generating sgRNA

Part Collection

2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explore a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.

We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".

The Collection includes:

dCas9-split luciferase Parts

Nluc-dCas9 (BBa_K1689010)
Cluc-dCas9 (BBa_K1689009)
dCas9-Nluc (BBa_K1689008)
dCas9-Cluc (BBa_K1689007)

dCas9-split Dihydrofolate Reductase Parts

F[1,2]-dCas9 (BBa_K1689015)
F3-dCas9 (BBa_K1689017)
dCas9-F[1,2] (BBa_K1689016)
dCas9-F3 (BBa_K1689018)

dCas9-split β–Lactamase Parts

dCas9-Nlact (BBa_K1689012)
Nlact-dCas9 (BBa_K1689013)
dCas9-Clact (BBa_K1689014)

dCas9-splitβ–Galactosidase Parts

Δα-dCas9 (BBa_K1689011)
dCas9-Δα (BBa_K1689019)
Δω-dCas9 (BBa_K1689020)

Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.