Difference between revisions of "Template:Team:TU Eindhoven/Timeline HTML"
| Line 16: | Line 16: | ||
<h3> - Preparing for take-off</h3> | <h3> - Preparing for take-off</h3> | ||
<br /> | <br /> | ||
| − | |||
<ul class="activiteitlijst"> | <ul class="activiteitlijst"> | ||
<li><span class="activiteit">Inventory of the lab supplies</span></li> | <li><span class="activiteit">Inventory of the lab supplies</span></li> | ||
| Line 31: | Line 30: | ||
<h3> - The Clone Wars </h3> | <h3> - The Clone Wars </h3> | ||
<br /> | <br /> | ||
| + | <span class="activiteit"> Gibson Assembly:</span> | ||
<ul class="activiteitlijst"> | <ul class="activiteitlijst"> | ||
<li><span class="activiteit">Linearizing pET-Duet-1 for Gibson Assembly </span></li> | <li><span class="activiteit">Linearizing pET-Duet-1 for Gibson Assembly </span></li> | ||
<li><span class="activiteit">Our first Gibson Assembly!</span></li> | <li><span class="activiteit">Our first Gibson Assembly!</span></li> | ||
| + | </ul> | ||
| + | <span class="activiteit"> Traditional cloning:</span> | ||
| + | <ul class="activiteitlijst"> | ||
<li><span class="activiteit">Amplification of the pET-Duet-1 vector</span></li> | <li><span class="activiteit">Amplification of the pET-Duet-1 vector</span></li> | ||
<li><span class="activiteit">Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning</span></li> | <li><span class="activiteit">Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning</span></li> | ||
Revision as of 07:24, 10 July 2015
Timeline
Week 26
- Preparing for take-off
- Inventory of the lab supplies
- Pouring LB Agar plates
- Amplification of the pET-Duet-1 vector
- Assessment of the safety requirements
- Preparing stocks for antibiotics, glycerol, LB & MilliQ
Week 27
- The Clone Wars
Gibson Assembly:
- Linearizing pET-Duet-1 for Gibson Assembly
- Our first Gibson Assembly!
- Amplification of the pET-Duet-1 vector
- Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning
Week 28
- Et tu, pET-Duet?
- Linearizing pET-Duet-1 for Gibson Assembly and debugging
- Amplification of the inserts for traditional cloning
- Xbal & Pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning
- Xba1 & Pst1 digestion of the inserts (MCS-1) for traditional cloning
- Nde1 & Kpn1 digestion of the inserts (MCS-2) for traditional cloning
- Ligation