Difference between revisions of "Template:Team:TU Eindhoven/Timeline HTML"
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<li><span class="activiteit">Our first Gibson Assembly!</span></li> | <li><span class="activiteit">Our first Gibson Assembly!</span></li> | ||
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<span class="activiteit"> Traditional cloning:</span> | <span class="activiteit"> Traditional cloning:</span> | ||
<ul class="activiteitlijst"> | <ul class="activiteitlijst"> | ||
Revision as of 07:25, 10 July 2015
Timeline
Week 26
- Preparing for take-off
- Inventory of the lab supplies
- Pouring LB Agar plates
- Amplification of the pET-Duet-1 vector
- Assessment of the safety requirements
- Preparing stocks for antibiotics, glycerol, LB & MilliQ
Week 27
- The Clone Wars
Gibson Assembly:
- Linearizing pET-Duet-1 for Gibson Assembly
- Our first Gibson Assembly!
Traditional cloning:
- Amplification of the pET-Duet-1 vector
- Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning
Week 28
- Et tu, pET-Duet?
- Linearizing pET-Duet-1 for Gibson Assembly and debugging
- Amplification of the inserts for traditional cloning
- Xbal & Pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning
- Xba1 & Pst1 digestion of the inserts (MCS-1) for traditional cloning
- Nde1 & Kpn1 digestion of the inserts (MCS-2) for traditional cloning
- Ligation