Difference between revisions of "Demo informationpage"

 
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{{2016Demo}}
 
{{2016Demo}}
 
<html>
 
<html>
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<style>
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.hide_info {width:25px; height:30px;background-color: #f2f2f2;text-align:center;font-weight:bold;padding-top:5px;cursor:pointer; left:975px; top:20px; position:absolute; z-index:50;}
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.hide_info:hover { background-color:orange; }
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/* STYLING AND LAYOUT - WIKI/CONTENT PAGES */
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/*Container for the image part of the top banner*/
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#topBanner{
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float:left;
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margin-left:20px;
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margin-bottom:-11px;
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}
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/*Container for the text side of the top banner */
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#topBannerText {
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float:left;
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width:700px;
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margin-left:30px;
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margin-top:35px;
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margin-bottom:-11px;
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}
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/*Styling for the text in topBannerText */
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.topBanner_synbio{
 +
font-size:38px;
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}
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.topBanner_parts{
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font-size:22px;
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}
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.content_toggle {
 +
float:left;margin-top:-30px; font-size: 20px; color: #5da88a; margin-left: 15px; font-weight:bold; cursor:pointer;
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}
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a.content_toggle { text-decoration:none; }
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a:active.content_toggle {  color: #5da88a; }
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</style>
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<div class="oneColumn">
 
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<h1>Team Information - Columbia NYC </h1>
 
−
</div>
 
  
 +
<div class="clear"></div>
 
<div class="clear"></div>
 
<div class="clear"></div>
  
−
<h2> General Information </h2>
+
<div id="topBanner">
 +
<a href="https://igem.org/Main_Page"><img class="imageHover" src="https://static.igem.org/mediawiki/2015/2/2a/Igemlogo_banner.jpg" height="120px"> </a>
 +
</div>
  
−
<div class="threeColumns">
+
<div id="topBannerText">
−
<p> School:<br>
+
<span class="topBanner_synbio"> <b> Synthetic Biology </b> </span>  
−
Middle East Technical University <br>
+
<br><br>
−
Ankara, Turkey <br>
+
<span class="topBanner_parts"> based on standard parts </span>
−
metu.edu.tr
+
</div>
−
</p>
+
 
 +
 
 +
 
 +
<div class="clear"></div>
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<div class="clear"></div>
 +
 
 +
<div class="hide_info"> X</div>
 +
 
 +
<div class="oneColumn" style="background-color:#f2f2f2;">
 +
<h1>Team Information  - Columbia NYC </h1>
 +
 
 +
 
 +
<div style="float:right;margin-right:20px;top:10px;margin-top:-40px;">
 +
<a href="">- wiki -</a>
 +
<a href=""> - parts - </a>
 +
<a href="">-  judging form -</a>
 +
 
 +
 
 +
<div class="toggle_control"><a href="#" > -  collapse all - </a></div>
 
</div>
 
</div>
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</div>
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<div class="clear"></div>
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<style>
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#contact_wrap, #general_wrap, #project_wrap, #awards_wrap, #deadlines_wrap1,  #deadlines_wrap2 {
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border: 1px solid #d3d3d3;
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border-right:none;
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float:left;
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margin-bottom: 0px;
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}
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.column_1, .column_3_side, .column_4_side {
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padding:15px;
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padding-bottom:10px;
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height: auto;
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border-right: 1px solid #d3d3d3;
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float:left;
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}
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.column_1 {
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margin:15px;
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width:915px;
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border: 1px solid #d3d3d3;
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}
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.column_3_side {
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width:284px;
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}
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.column_4_side {
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width:206px;
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}
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.column_1 ul li, .column_3_side ul li, .column_4_side ul li {
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list-style:none;
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margin-left:-15px;
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}
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.column_1 ol li, .column_3_side ol li, .column_4_side ol li { margin-left:-15px; }
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.edit_button{
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float:right;
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background-color: white;
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margin:-10px;
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padding:5px;
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padding-right:12px;
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color:orange;
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font-weight:bold;
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cursor:pointer;
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}
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.edit_button:hover{
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background-color: orange;
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color:white;
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}
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.title_section {
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height:20px;
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float:left;
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margin:-15px;
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padding:10px;
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padding-left:15px;
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}
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.clear_line {
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margin-bottom: 15px;
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height: 20px;
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border-bottom: 1px solid #d3d3d3;
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clear: both;
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}
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 +
.title_section b {
 +
font-size:14px;
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}
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.roster_name, .roster_user { width:20%; float:left; }
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.roster_email { width:30%; float:left; }
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.roster_gender, .roster_age { width:10%; float:left; }
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.roster_edit { margin-right: 10px; padding-top: 5px;}
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.roster_team_member {    height: 25px; padding-top: 10px; }
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</style>
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 +
<div id="general">
 +
<div class="clear" style="height:90px;"></div>
 +
<a class="content_toggle" >▼ General</a>
 +
 +
<div class="toggle_collapse">
 +
 +
<div id="general_wrap">
 +
 +
<div class="threeColumns">
 +
 +
<div class="title_section">
 +
<b> Schools </b> 
 +
<span class="pop_why" onclick="which_why='why_schools'">?</span> 
 +
</div>
 +
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
 +
<div class="clear_line"></div>
 +
 +
<ul>
 +
<li> Stanford University Online High School, CA, USA
 +
<A HREF="">ohs.stanford.edu </a></LI>
 +
<li>Hankuk Academy of Foreign Studies Kyunggi, Korea
 +
<A HREF="">www.hafs.hs.kr  </a></LI>
 +
<li>Cheongshim International Academy, Kyunggi, Korea
 +
<A HREF="">http://www.csia.hs.kr/ </a> </LI>
 +
</ul>
 +
</div>
 +
 +
 +
<div class="threeColumns">
 +
 +
 +
<div class="title_section">
 +
<b> Team Status</b> 
 +
<span class="pop_why" onclick="which_why='why_status'">?</span>   
 +
</div>
 +
 +
 +
<div class="clear_line"></div>
 +
 +
 +
<ul>
 +
<li> <span style="font-size:20px;color:green;"> ✔  </span>  Your <a href="">registration fee</a> has been received </li>
 +
<li>  <span style="font-size:20px;color:green;"> ✔  </span>  <a href="">Shipping</a> information has been entered</li>
 +
<li> <span style="font-size:20px;color:green;"> ✔  </span>  <a href="">Resource description</a> has been submitted </li>
 +
</ul>
 +
 +
<span style="font-size:15px;color:green; padding-top:10px;">Team approved </span>
 +
</div>
 +
 +
 +
 +
<div class="threeColumns">
 +
 +
<div class="title_section">
 +
<b> Location</b> 
 +
</div>
 +
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
 +
 +
<div class="clear_line"></div>
 +
 +
<ul>
 +
<li>Section: <span class="pop_why" onclick="which_why='why_sections'">?</span>  Undergraduate </li>
 +
<li> Region:  Europe </li>
 +
<li> Application Date:  2015-02-21 </li>
 +
<li>Acceptance Date: 2015-04-13 14:21:14 </li>
 +
</ul>
 +
 +
 +
</div>
  
  
−
<div class="threeColumns">
 
−
<p>Section: Undergraduate
 
−
<br>
 
−
Region: Europe
 
−
</p>
 
 
</div>
 
</div>
  
  
  
−
<div class="threeColumns">
 
−
<p>Application Date:  2015-02-21
 
−
<br>
 
−
Acceptance Date: 2015-04-13 14:21:14
 
−
</p>
 
 
</div>
 
</div>
  
 +
</div>
 +
 +
 +
 +
 +
 +
<div id="contact_information">
 +
 +
<div class="clear" style="height:60px;"></div>
 +
 +
<a class="content_toggle" >▼Contact</a>
 +
 +
<div class="toggle_collapse">
 +
 +
<div id="contact_wrap">
 +
 +
<div class="threeColumns">
 +
 +
<div class="title_section">
 +
<b> Primary Instructor (PI) </b> 
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
 +
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
 +
<div class="clear_line"></div>
 +
 +
<div> <p><div style="width:50px;float:left;"> <span style="font-size:30px; text-align:center;">☺ </span>  </div>  Jiandong Jiang</p></div>
 +
 +
 +
<div class="private_info"> <p><div  style="width:50px;float:left;"> <span style="font-size:30px; text-align:center; width:30px;">✆  </span> </div> +3472349823749</p></div>
 +
 +
 +
<div class="private_info"> <p><div style="width:50px;float:left;"> <span style="font-size:30px; text-align:center; width:30px;">✉  </span> </div>  @njau.edu.cn</p></div>
 +
 +
</div>
 +
 +
 +
<div class="threeColumns">
 +
 +
 +
<div class="title_section">
 +
<b> Secondary Instructor</b> 
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
 +
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
 +
<div class="clear_line"></div>
 +
 +
<div> <p><div style="width:50px;float:left;"> <span style="font-size:30px; text-align:center;">☺ </span>  </div>  Jiandong Jiang</p></div>
 +
 +
 +
<div class="private_info"> <p><div  style="width:50px;float:left;"> <span style="font-size:30px; text-align:center; width:30px;">✆  </span> </div> +3472349823749</p></div>
 +
 +
 +
<div class="private_info"> <p><div style="width:50px;float:left;"> <span style="font-size:30px; text-align:center; width:30px;">✉  </span> </div>  @njau.edu.cn</p></div>
 +
 +
</div>
 +
 +
 +
 +
<div class="threeColumns">
 +
 +
<div class="title_section">
 +
<b> Student Leader </b> 
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
 +
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
 +
<div class="clear_line"></div>
 +
 +
<div> <p><div style="width:50px;float:left;"> <span style="font-size:30px; text-align:center;">☺ </span>  </div>  Jiandong Jiang</p></div>
 +
 +
 +
<div class="private_info"> <p><div  style="width:50px;float:left;"> <span style="font-size:30px; text-align:center; width:30px;">✆  </span> </div> +3472349823749</p></div>
 +
 +
 +
<div class="private_info"> <p><div style="width:50px;float:left;"> <span style="font-size:30px; text-align:center; width:30px;">✉  </span> </div>  @njau.edu.cn</p></div>
 +
 +
</div>
  
−
<div class="oneColumn" style="background-color:#f2f2f2;">
 
−
<p> Description: description is optional </p>
 
  
 +
</div>
 +
</div>
 
</div>
 
</div>
  
  
−
<div class="clear"></div>
+
<div class="clear" style="height:90px;"></div>
 +
 
 +
 
 +
<a class="content_toggle" >▼ Deadlines</a>
 +
 
 +
<div class="toggle_collapse">
 +
 
 +
<div id="deadlines_wrap1">
 +
 
 +
<div class="threeColumns">
 +
 
 +
<div class="title_section">
 +
<b> May</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<ul>
 +
<li> <span style="font-size:20px;color:green;"> ✔  </span>  <b>10</b> <a href="">Check In Form</a></li>
 +
</ul>
 +
</div>
 +
 
 +
 
 +
<div class="threeColumns">
 +
 +
 
 +
<div class="title_section">
 +
<b> June</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<ul>
 +
<li> <span style="font-size:20px;color:orange;"> !  </span> <b>10</b> <a href="">Visa Application Day</a></li>
 +
<li> <span style="font-size:20px;color:orange;"> !  </span><b> 19</b> <a href="">InterLab Measurement study</a> sign up</li>
 +
<li> <span style="font-size:20px;color:green;"> ✔  </span>  <b> 22</b> <a href="">About our Lab form</a></li>
 +
<li>  <span style="font-size:20px;color:green;"> ✔  </span>  <b>25</b> <a href="">About our Project form</a></li>
 +
</ul>
 +
</div>
 +
 
 +
 
 +
 
 +
<div class="threeColumns">
 +
 
 +
<div class="title_section">
 +
<b> July</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
  
−
<h2> Contact Information </h2>
+
<ul>
 +
<li> <span style="font-size:20px;color:red;"> ✘  </span>  <b>15</b> <a href="">About our Project form</a></li>
 +
<li> <span style="font-size:20px;color:red;"> ✘  </span>  <b>15</b> <a href="">Project description</a>due</li>
 +
</ul>
  
−
<div class="threeColumns">
 
  
−
<p>  <b>Student Leader</b>
+
</div>
−
<br>
+
−
Jiandong Jiang
+
−
<br>
+
−
+3472349823749
+
−
<br>
+
−
@njau.edu.cn
+
−
</p>
+
 
</div>
 
</div>
  
  
−
<div class="threeColumns">
+
<div id="deadlines_wrap2">
 +
<div class="threeColumns">
  
−
<p>  <b>Primary Contact (PI) </b>
+
<div class="title_section">
−
<br>
+
<b> August</b>   
−
Alvaro Olivera-Nappa
+
</div>
−
<br>
+
 
−
+3472349823749
+
<div class="clear_line"></div>
−
<br>
+
 
−
@ing.uchile.cl
+
<ul>
−
</p>
+
<li> <span style="font-size:20px;color:red;"> ✘  </span>  <b>07</b> <a href="">Track selection</a> due</li>
 +
<li> <span style="font-size:20px;color:red;"> ✘  </span>  <b>07</b> <a href="">Title and abstract</a>due</li>
 +
<li> <span style="font-size:20px;color:green;"> ✔  </span>  <b> 28</b> <a href="">Final Safety form</a></li>
 +
</ul>
 +
</div>
 +
 
 +
 
 +
<div class="threeColumns">
 +
 
 +
<div class="title_section">
 +
<b> September</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<ul>
 +
 
 +
<li> <span style="font-size:20px;color:orange;"> !  </span> <b>10</b> <a href="">Team banners</a> due</li>
 +
<li> <span style="font-size:20px;color:green;"> ✔  </span>  <b> 11</b> <a href="">Team rosters</a>due</li>
 +
<li> <span style="font-size:20px;color:red;"> ✘  </span>  <b>18</b> <a href="">Part submission</a> send by date</li>
 +
<li> <span style="font-size:20px;color:red;"> ✘  </span>  <b>18</b> <a href="">Judging form</a> due</li>
 +
<li> <span style="font-size:20px;color:orange;"> !  </span> <b>18</b> <a href="">Wiki freeze</a></li>
 +
<li> <span style="font-size:20px;color:green;"> ✔  </span>  <b> 11</b> <a href="">Keywords</a>due</li>
 +
</ul>
 +
</div>
 +
 
 +
 
 +
<div class="threeColumns">
 +
 
 +
<div class="title_section">
 +
<b> October</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
<ul>
 +
<li> <span style="font-size:20px;color:orange;"> !  </span> <b>31</b> <a href="">Wiki freeze archive</a></li>
 +
</ul>
 +
</div>
 +
 
 +
 
 +
</div>
 
</div>
 
</div>
  
  
−
<div class="threeColumns">
 
  
−
<p> <b>Primary Contact (PI) </b>
+
<div class="clear" style="height:90px;"></div>
−
<br>
+
 
−
Harris Wang
+
 
−
<br>
+
<a class="content_toggle" >▼ Project</a>
−
+3472349823749
+
 
−
<br>
+
<div class="toggle_collapse">
−
@columbia.edu
+
<div id="project_wrap">
−
</p>
+
 
 +
<div class="fourColumns">
 +
 
 +
<div class="title_section">
 +
<b> Assigned Track</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<p>New Application</p>
 +
</div>
 +
 
 +
<div class="fourColumns">
 +
<div class="title_section">
 +
<b> Track Preferences</b>
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
 +
 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<ol>
 +
<li>New Application </li>
 +
<li>Manufacturing</li>
 +
<li>Measurement </li>
 +
</ol>
 +
</div>
 +
 
 +
 
 +
<div class="fourColumns">
 +
<div class="title_section">
 +
<b> Part Numbers</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<p>BBa_K1606000 <br>
 +
to <br>
 +
BBa_K1606999<br>
 +
</p>
 +
</div>
 +
 
 +
<div class="fourColumns">
 +
<div class="title_section">
 +
<b> Keywords</b> 
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
 +
 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<ol>
 +
<li>Insects </li>
 +
<li>toolkit </li>
 +
<li>Spodoptera frugiperda</li>
 +
</ol>
 +
</div>
 
</div>
 
</div>
  
 +
<div class="oneColumn column_gray">
 +
<div class="title_section">
 +
<b> Title and Abstract</b> 
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
  
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
  
 +
<div class="clear_line"></div>
  
−
<div class="clear"></div>
+
<p> The purpose of this project is to introduce the Sf9 cell line (Fall armyworm ovary, Spodoptera frugiperda cells) to synthetic biology and the iGEM Competition, by developing a tool kit of several biobricks for their use in this new chassis. We will use the Sf9 cells as an alternative for the production of proteins with high complexity and post-traductional modifications, because of their flexibility, cultivation time and minor cost compared with other expression systems. We will characterize functional parts for two main areas: Transfection and Genome Editing. For the first part we will characterize baculovirus mediated transfection and, direct plasmid transfection for the generation of stable cell lines; for the second part we will use the CRISPR/Cas9 system to assay highly specific genome modifications in an effort to improve production and ease of use of this technology. </p>
−
<h2> Team Status </h2>
+
 
 +
 
 +
</div>
  
−
<div class="twoColumns">
 
−
<ol>
 
−
<li>DNA Kit of Parts shipping information has been entered</li>
 
−
<li>Resource description has been submitted</li>
 
−
<li>Your registration fee has been received</li>
 
−
<li>DNA Kit of Parts shipping information has been entered</li>
 
−
<li>Resource description has been submitted</li>
 
−
<li>Your registration fee has been received</li>
 
−
<li>DNA Kit of Parts shipping information has been entered</li>
 
−
<li>Resource description has been submitted</li>
 
−
<li>Your registration fee has been received</li>
 
−
</ol>
 
 
</div>
 
</div>
  
 +
<div class="clear" style="height:90px;"></div>
  
  
−
<div class="twoColumns">
+
<a class="content_toggle" >▼ Roster </a>
  
 +
<div class="toggle_collapse">
  
 +
 +
<div class="oneColumn">
 +
<div class="title_section">
 +
<b> Instructors</b> 
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
 +
 +
<div class="clear_line"></div>
 +
 +
<div class="roster_team_member">
 +
 +
<div class="roster_name">
 +
Name LastName
 +
</div> 
 +
 +
<div class="roster_user"> 
 +
<a href="">username </a>
 +
</div> 
 +
 +
 +
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div> 
 +
 +
 +
<div class="roster_age"> 
 +
[N/A]
 +
</div>
 +
 +
 
 +
<div class="roster_gender"> 
 +
male
 +
</div> 
 +
 +
<div class="roster_edit"> 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
</div>
 
</div>
 
</div>
  
  
  
−
<div class="clear"></div>
+
<div class="roster_team_member">
 +
<div class="roster_name">
 +
Name LastName
 +
</div>
  
 +
<div class="roster_user"> 
 +
<a href="">username </a>
 +
</div> 
  
  
 +
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div> 
  
  
−
<h2> Project</h2>
+
<div class="roster_age">  
 +
[N/A]
 +
</div>
  
−
<div class="fourColumns">
+
 
−
<h4>Assigned Track</h4>
+
<div class="roster_gender">  
 +
male
 +
</div>
  
−
<p>New Application</p>
+
<div class="roster_edit">
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
</div>  
 
</div>
 
</div>
  
 +
<div class="roster_team_member">
  
−
<div class="fourColumns">
+
<div class="roster_name">  
−
<h4>Track Preferences </h4>
+
Name LastName
 +
</div>
  
−
<ol>
+
<div class="roster_user">
−
<li>New Application </li>
+
<a href="">username </a>
−
<li>Manufacturing</li>
+
</div>
−
<li>Measurement </li>
+
 
−
</ol>
+
 
 +
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div>
 +
 
 +
 
 +
<div class="roster_age">  
 +
[N/A]
 +
</div>
 +
 
 +
 
 +
<div class="roster_gender"> 
 +
male
 +
</div> 
 +
 
 +
<div class="roster_edit"> 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>  
 +
</div>  
 
</div>
 
</div>
  
 +
</div>
  
  
−
<div class="fourColumns">
+
<div class="oneColumn">
−
<h4>Part Numbers </h4>
+
<div class="title_section">
 +
<b> Students </b> 
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
  
−
<p>BBa_K1606000 <br>
+
<div class="clear_line"></div>
−
to <br>
+
 
−
BBa_K1606999<br>
+
<div class="roster_team_member">
−
</p>
+
 
 +
<div class="roster_name">
 +
Name LastName
 +
</div> 
 +
 
 +
<div class="roster_user"> 
 +
<a href="">username </a>
 +
</div> 
 +
 
 +
 
 +
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div> 
 +
 
 +
 
 +
<div class="roster_age"> 
 +
[N/A]
 +
</div>
 +
 
 +
 
 +
<div class="roster_gender"> 
 +
male
 +
</div> 
 +
 
 +
<div class="roster_edit"> 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>  
 +
</div>  
 
</div>
 
</div>
  
  
−
<div class="fourColumns">
 
−
<h4> Keywords </h4>
 
  
−
<ol>
+
<div class="roster_team_member">
−
<li>Insects </li>
+
<div class="roster_name">  
−
<li>toolkit </li>
+
Name LastName
−
<li>Spodoptera frugiperda</li>
+
</div>
−
</ol>
+
 
 +
<div class="roster_user">
 +
<a href="">username </a>
 +
</div>
 +
 
 +
 
 +
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div> 
 +
 
 +
 
 +
<div class="roster_age"> 
 +
[N/A]
 +
</div>
 +
 
 +
 
 +
<div class="roster_gender"> 
 +
male
 +
</div> 
 +
 
 +
<div class="roster_edit"> 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>  
 +
</div>  
 
</div>
 
</div>
  
−
<div class="oneColumn" style="background-color:#f2f2f2;">
+
<div class="roster_team_member">
  
−
<h3>Title and Abstract </h3>
+
<div class="roster_name">  
 +
Name LastName
 +
</div>
  
−
<h4> Insects join iGEM: Sf9 cells as a new chassis for synthetic biology </h4>
+
<div class="roster_user">
 +
<a href="">username </a>
 +
</div>
  
−
<p>
 
−
The purpose of this project is to introduce the Sf9 cell line (Fall armyworm ovary, Spodoptera frugiperda cells) to synthetic biology and the iGEM Competition, by developing a tool kit of several biobricks for their use in this new chassis. We will use the Sf9 cells as an alternative for the production of proteins with high complexity and post-traductional modifications, because of their flexibility, cultivation time and minor cost compared with other expression systems. We will characterize functional parts for two main areas: Transfection and Genome Editing. For the first part we will characterize baculovirus mediated transfection and, direct plasmid transfection for the generation of stable cell lines; for the second part we will use the CRISPR/Cas9 system to assay highly specific genome modifications in an effort to improve production and ease of use of this technology.
 
−
</p>
 
  
 +
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div> 
 +
 +
 +
<div class="roster_age"> 
 +
[N/A]
 +
</div>
 +
 +
 
 +
<div class="roster_gender"> 
 +
male
 +
</div> 
 +
 +
<div class="roster_edit"> 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
</div>
 
</div>
 
</div>
  
  
−
<div class="clear"></div>
+
</div>
  
  
−
<h2>Team Roster </h2>
+
<div class="oneColumn">
 +
<div class="title_section">
 +
<b> Advisors</b> 
 +
<span class="pop_why" onclick="which_why='default'">?</span> 
 +
</div>
  
−
<div class="oneColumn">
+
<div class="clear_line"></div>
−
<p> As a leader, you may add a member to this team unilaterally: Add a member </p>
+
 
 +
<div class="roster_team_member">
 +
 
 +
<div class="roster_name">
 +
Name LastName
 +
</div> 
 +
 
 +
<div class="roster_user"> 
 +
<a href="">username </a>
 +
</div> 
 +
 
 +
 
 +
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div> 
 +
 
 +
 
 +
<div class="roster_age"> 
 +
[N/A]
 +
</div>
 +
 
 +
 
 +
<div class="roster_gender"> 
 +
male
 +
</div>
  
 +
<div class="roster_edit"> 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
</div>
 
</div>
 
</div>
  
  
−
<div style="width:20%; float:left;">
 
−
Name LastName <bR>
 
−
Name LastName <bR>
 
−
Name LastName <bR>
 
−
</div> 
 
  
−
<div style="width:20%; float:left;">
+
<div class="roster_team_member">
−
username <br>
+
<div class="roster_name">  
−
username <br>
+
Name LastName
−
username <br>
+
</div>   
−
</div>   
+
  
 +
<div class="roster_user"> 
 +
<a href="">username </a>
 +
</div> 
  
−
<div style="width:30%; float:left;"> 
 
−
hjsdjfjskfkjs@gmail.com <br>
 
−
hjsdjfjskfkjs@gmail.com <br>
 
−
hjsdjfjskfkjs@gmail.com <br>
 
  
−
</div>   
+
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div>   
  
  
−
<div style="width:10%; float:left;">
+
<div class="roster_age">  
−
[N/A] <br>
+
[N/A]  
−
20<br>
+
</div>
−
19<br>
+
 
 +
 
 +
<div class="roster_gender">  
 +
male
 +
</div> 
 +
 
 +
<div class="roster_edit"> 
 +
<div class="edit_button">
 +
<span style="font-size:16px;"> ✎ </span> edit 
 +
</div>
 +
</div>  
 
</div>
 
</div>
 +
 +
<div class="roster_team_member">
 +
 +
<div class="roster_name">
 +
Name LastName
 +
</div> 
 +
 +
<div class="roster_user"> 
 +
<a href="">username </a>
 +
</div> 
 +
 +
 +
<div class="roster_email"> 
 +
hjsdjfjskfkjs@gmail.com
 +
</div> 
 +
 +
 +
<div class="roster_age"> 
 +
[N/A]
 +
</div>
  
 
    
 
    
−
<div style="width:10%; float:left;">
+
<div class="roster_gender">  
−
male <br>
+
male  
−
female <br>
+
</div>   
−
other <br>
+
−
</div>   
+
  
−
<div style="width:10%; float:left;">   
+
<div class="roster_edit">   
−
edit <br>
+
<div class="edit_button">
−
edit <br>
+
<span style="font-size:16px;"> ✎ </span> edit
−
edit <br>
+
</div>  
−
</div>
+
</div>  
 +
</div>
  
 +
</div>
  
 +
</div>
  
−
<div class="clear"></div>
+
<div class="clear" style="height:90px;"></div>
 +
 
 +
 
 +
<a class="content_toggle" >▼ Awards</a>
 +
 
 +
<div class="toggle_collapse">
 +
<div id="awards_wrap">
 +
 
 +
<div class="threeColumns">
 +
 
 +
<div class="title_section">
 +
<b> Medals</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<p><img src="https://igem.org/images/medals/seal_gold.png">  Gold </p>
 +
 
 +
</div>
 +
 
 +
 
 +
<div class="threeColumns">
 +
 
 +
<div class="title_section">
 +
<b> Awards</b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
<p> Best Manufacturing Project, Overgrad</p>
 +
</div>
 +
 
 +
 
 +
 
 +
<div class="threeColumns">
 +
 
 +
<div class="title_section">
 +
<b> Nominations </b> 
 +
</div>
 +
 
 +
<div class="clear_line"></div>
 +
 
 +
 
 +
<p> Best Manufacturing Project, Overgrad</p>
 +
<p> Best Software Tool, Overgrad </p>
 +
<p>Best Part Collection, Overgrad</p>
 +
 
 +
 
 +
</div>
 +
 
 +
 
 +
</div>
 +
</div>
 +
<div class="clear" style="height:90px;"></div>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<div class="clear" style="height:30px;"></div><div class="clear" style="height:30px;"></div><div class="clear" style="height:30px;"></div>
 +
 
 +
 
 +
<script type="text/javascript">
 +
 
 +
 
 +
 
 +
 
 +
 
 +
$(".content_toggle").click(function() {
 +
jQuery(this).next("div").slideToggle();
 +
 
 +
});
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
$("#general_wrap .column_3_side").css( "height", ($('#general_wrap').height() -20)  );
 +
 
 +
$("#contact_wrap .column_3_side").css( "height", ($('#contact_wrap').height() -20)  );
 +
 
 +
$("#project_wrap .column_4_side").css( "height", ($('#project_wrap').height() -20)  );
 +
 
 +
$("#awards_wrap .column_3_side").css( "height", ($('#awards_wrap').height() -20)  );
 +
 
 +
$("#deadlines_wrap1 .column_3_side").css( "height", ($('#deadlines_wrap1').height() -20)  );
 +
 
 +
$("#deadlines_wrap2 .column_3_side").css( "height", ($('#deadlines_wrap2').height() -20)  );
 +
 
 +
 
 +
 
 +
 
 +
 
 +
var testing_if = false;
 +
 
 +
$(".hide_info").click(function() {
 +
 +
if (testing_if == false) {
 +
 
 +
testing_if = true;
 +
$(".pop_why").hide();
 +
$(".edit_button").hide();
 +
$(".private_info").hide();
 +
}
 +
 
 +
// to close
 +
else if (testing_if == true) {
 +
$(".pop_why").show();
 +
$(".edit_button").show();
 +
$(".private_info").show();
 +
testing_if = false;
 +
}
 +
 
 +
});
 +
 
 +
 
 +
 
 +
 
 +
var testing_toggle= false;
 +
 
 +
$(".toggle_control").click(function() {
 +
 +
if (testing_toggle == false) {
 +
 
 +
testing_toggle = true;
 +
$(".toggle_collapse").hide();
 +
$(".toggle_control").html("<a href='#'> +  expand all + </a>");
 +
 
 +
}
 +
 
 +
// to close
 +
else {
 +
$(".toggle_collapse").show();
 +
testing_toggle = false;
 +
$(".toggle_control").html("<a href='#' > -  collapse all - </a>");
 +
}
 +
 
 +
});
 +
 
 +
 
 +
 
 +
</script>
  
  
 
</html>
 
</html>
 +
 +
 +
 
{{2016Demo_bottom}}
 
{{2016Demo_bottom}}

Latest revision as of 22:19, 17 November 2015

×

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Synthetic Biology

based on standard parts
X

Team Information - Columbia NYC

▼ General
Schools ?
✎ edit
Team Status ?
Team approved
Location
✎ edit
  • Section: ? Undergraduate
  • Region: Europe
  • Application Date: 2015-02-21
  • Acceptance Date: 2015-04-13 14:21:14
▼Contact
Primary Instructor (PI) ?
✎ edit

☺
Jiandong Jiang

✆
+3472349823749

✉
@njau.edu.cn

Secondary Instructor ?
✎ edit

☺
Jiandong Jiang

✆
+3472349823749

✉
@njau.edu.cn

Student Leader ?
✎ edit

☺
Jiandong Jiang

✆
+3472349823749

✉
@njau.edu.cn

▼ Deadlines
August
September
October
▼ Project
Assigned Track

New Application

Track Preferences ?
✎ edit
  1. New Application
  2. Manufacturing
  3. Measurement
Part Numbers

BBa_K1606000
to
BBa_K1606999

Keywords ?
✎ edit
  1. Insects
  2. toolkit
  3. Spodoptera frugiperda
Title and Abstract ?
✎ edit

The purpose of this project is to introduce the Sf9 cell line (Fall armyworm ovary, Spodoptera frugiperda cells) to synthetic biology and the iGEM Competition, by developing a tool kit of several biobricks for their use in this new chassis. We will use the Sf9 cells as an alternative for the production of proteins with high complexity and post-traductional modifications, because of their flexibility, cultivation time and minor cost compared with other expression systems. We will characterize functional parts for two main areas: Transfection and Genome Editing. For the first part we will characterize baculovirus mediated transfection and, direct plasmid transfection for the generation of stable cell lines; for the second part we will use the CRISPR/Cas9 system to assay highly specific genome modifications in an effort to improve production and ease of use of this technology.

▼ Roster
Instructors ?
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
Students ?
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
Advisors ?
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
Name LastName
username
hjsdjfjskfkjs@gmail.com
[N/A]
male
✎ edit
▼ Awards
Medals

Gold

Awards

Best Manufacturing Project, Overgrad

Nominations

Best Manufacturing Project, Overgrad

Best Software Tool, Overgrad

Best Part Collection, Overgrad