Difference between revisions of "Template:Team:Groningen/CONTENT/Home/Blogs"
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|title=Rigidity of the biofilm | |title=Rigidity of the biofilm | ||
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| − | To survive water flow the biofilm has to be stable and robust. | + | To survive water flow the biofilm has to be stable and robust. Genes encoding biofilm proteins must be overexpressed and the stability of the biofilm must be improved by knocking out the genetic pathways that result in cell motility and biofilm repression. |
</html> | </html> | ||
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|title=New shuttle vector | |title=New shuttle vector | ||
|content=<html> | |content=<html> | ||
| − | The BBa_K823023 backbone is one of the standard backbones for | + | The BBa_K823023 backbone is one of the standard backbones for <i>Bacillus</i> for integrating in the <i>amyE</i> locus. However, an extra integration locus is welcome when making a multiple mutant. Another integration locus is the <i>thrC</i> locus. To create this backbone the BBa_K823023 backbone was used, where the <i>amyE</i> locus parts were replaced with the <i>thrC</i> locus parts from the plasmid pDG1664. This resulted in a new shuttle vector for <i>B. subtilis</i>. |
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{{Template:Team:Groningen/TEMPLATES/READMOREBLOG | {{Template:Team:Groningen/TEMPLATES/READMOREBLOG | ||
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| − | |title=Human | + | |title=Human Practices |
|content=<html> | |content=<html> | ||
| − | When working with | + | When working with GMOs it is important to know the regulations and think about future perspectives, which we did by considering possible future scenarios. Also it is important to talk about iGEM and your project at events, with several media, at this occasions we used our own card game. |
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|title=Future perspective | |title=Future perspective | ||
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| − | To implement our project the GMOs need to stay in the power plant. Therefore we thought about | + | To implement our project the GMOs need to stay in the power plant. Therefore we thought about trapping the bacteria between two carriers, about antimicrobial layers and a kill switch. In addition to not escaping, the ultimate goal is that the biofilm can sustain itself by using nutrients present in the water. |
</html> | </html> | ||
}} | }} | ||
Revision as of 16:27, 19 November 2015
<img class="image" src="#"/>
Carrier on which the biofilm grows
Our biofilm was grow on a carrier material for strength and durability. Whatman paper was chosen for its great biofilm growth and low cost. Four growth methods were studied to optimize biofilm growth and strength.
<img class="image" src="#"/>
Biofilm ion selectivity
The γ-PGA molecule was modelled using Molecular Dynamics. Wetlab testing was performed using a Natto strain of Bacillus subtilis.
<img class="image" src="#"/>
Rigidity of the biofilm
To survive water flow the biofilm has to be stable and robust. Genes encoding biofilm proteins must be overexpressed and the stability of the biofilm must be improved by knocking out the genetic pathways that result in cell motility and biofilm repression.
<img class="image" src="#"/>
New shuttle vector
The BBa_K823023 backbone is one of the standard backbones for Bacillus for integrating in the amyE locus. However, an extra integration locus is welcome when making a multiple mutant. Another integration locus is the thrC locus. To create this backbone the BBa_K823023 backbone was used, where the amyE locus parts were replaced with the thrC locus parts from the plasmid pDG1664. This resulted in a new shuttle vector for B. subtilis.
<img class="image" src="#"/>
Human Practices
When working with GMOs it is important to know the regulations and think about future perspectives, which we did by considering possible future scenarios. Also it is important to talk about iGEM and your project at events, with several media, at this occasions we used our own card game.
<img class="image" src="#"/>
Future perspective
To implement our project the GMOs need to stay in the power plant. Therefore we thought about trapping the bacteria between two carriers, about antimicrobial layers and a kill switch. In addition to not escaping, the ultimate goal is that the biofilm can sustain itself by using nutrients present in the water.