Difference between revisions of "Team:Vanderbilt/Project/Achievements"

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</div>
 
</div>
 
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</html>
 
 
{{Template:Vanderbilt_Navbar}}
 
{{Template:Vanderbilt_Navbar}}
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<html>
 
<html>
  
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<style>
 
 
html, body {
 
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Revision as of 16:45, 19 November 2015

Vanderbilt iGEM 2015


Sequence

  • Invented an innovative software tool for minimizing any gene’s susceptibility to mutation
  • Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques
  • Experimentally validated decreased mutation in optimized sequences with
    • Multiple mutagen types (UV radiation, oxidation)
    • Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)
  • Established high expression of optimized sequences
  • Modeling and computational strategies for further improvements and expansion of software
  • Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection

Organism

  • Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits
  • Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements
  • Developed assays for measuring decreased recombination with homology-minimization software
  • Constructed optimized circuit to demonstrate improved stability with VERT technique
  • Validated bidirectional promoter for use with system to select against promoter mutation

Circuit

  • Cloned five exogenous DNA repair enzymes
  • Incorporated KIKO vector for genomic integration and simultaneous knock-out
  • Designed “Incorruptible Cell” that commits suicide instead of passing on mutations