Difference between revisions of "Team:Vanderbilt/Project/Achievements"

Sequence

• Invented an innovative software tool for minimizing any gene’s susceptibility to mutation
• Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques
• Experimentally validated decreased mutation in optimized sequences with
• Multiple mutagen types (UV radiation, oxidation)
• Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)
• Established high expression of optimized sequences
• Modeling and computational strategies for further improvements and expansion of software
• Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection

Organism

• Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits
• Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements
• Developed assays for measuring decreased recombination with homology-minimization software
• Constructed optimized circuit to demonstrate improved stability with VERT technique
• Validated bidirectional promoter for use with system to select against promoter mutation

Circuit

• Cloned five exogenous DNA repair enzymes
• Incorporated KIKO vector for genomic integration and simultaneous knock-out
• Designed “Incorruptible Cell” that commits suicide instead of passing on mutations