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Revision as of 18:08, 19 November 2015
Demons in the Code
Sequence
- Invented an innovative software tool for minimizing any gene’s susceptibility to mutation
- Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques
- Experimentally validated decreased mutation in optimized sequences with
- Multiple mutagen types (UV radiation, oxidation)
- Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)
- Established high expression of optimized sequences
- Modeling and computational strategies for further improvements and expansion of software
- Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection
Organism
- Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits
- Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements
- Developed assays for measuring decreased recombination with homology-minimization software
- Constructed optimized circuit to demonstrate improved stability with VERT technique
- Validated bidirectional promoter for use with system to select against promoter mutation
Circuit
- Cloned five exogenous DNA repair enzymes
- Incorporated KIKO vector for genomic integration and simultaneous knock-out
- Designed “Incorruptible Cell” that commits suicide instead of passing on mutations