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<h1 align="center">Never Too LATE:</h1>
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<h1 align="center">Fight the BLIGHT</h1>
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<h2>Abstract</h2>
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<p>Phytophthora infestans is the causal agent of late blight disease of several members from the Solanaceae family. Potato, the third most important food crop in the world and one of the sources of major agricultural income in many countries, easily falls victim to P. infestans when temperature and moisture is suitable. Yet most existing biological control methods are ineffective and may even have further negative effects. This year, the NYMU-Taipei iGEM team creates a new systematic way to fight against potato late blight. We have characterized a new defensin in order to block the nutrient absorption and further growth of the oomycete. Inspired by competitive inhibition in pharmacology, we designed and improved a ligand with higher affinity aiding the entrance of P. infestans avirulence protein. To detect whether the potato is susceptible to late blight, we devised a soil-based microbial fuel cell (MFC) detecting salicylic acid emission and producing oscillating current. In our project design, we strive to cover every aspect that can prevent, fight against, and detect potato late blight. Furthermore, we will provide a standard procedure that can easily be followed by anyone without advanced knowledge in biology.</p>
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                  <div align="center" class="maincontext">Fight the Blight</div>
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                  <div align="center" class="maincontext" style="font-size:30px">Defending the potatoes</div>
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<div align="center" class="subcontext">&ensp;&ensp;&ensp;&ensp;The 2015 NYMU iGEM team presents a potato defense system, an effective system that can be easily implemented by potato farmers all over the world. This system is made up of three parts: protection, detection, and cure.</div>
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&ensp;&ensp;&ensp;&ensp;Our system consists of potatoes resistant to <i>Phytophthora infestans</i>, an oomycete that causes potato late blight, by creating a competitive inhibitor that can prevent the effector protein secreted by <i>P. infestans</i> from entering the potato cell. In case that the potatoes we genetically modified cannot fend off the <i>P. infestans</i>, we designed a soil based microbial fuel cell (SMFC) with engineered bacteria on the anode of the SMFC that can detect whether the potato tuber is infected or not. If the potato is infected and detected by our SMFC, we will spray defensin, an antimicrobial peptide isolated from maca (<i>Lepidium meyenii</i>). The defensin can weaken and inhibit the growth of <i>P. infestans</i> without doing harm to the environment and human body since it does not contain heavy metals like most fungicides.
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Latest revision as of 11:57, 20 November 2015

Fight the Blight
Defending the potatoes
    The 2015 NYMU iGEM team presents a potato defense system, an effective system that can be easily implemented by potato farmers all over the world. This system is made up of three parts: protection, detection, and cure.
    Our system consists of potatoes resistant to Phytophthora infestans, an oomycete that causes potato late blight, by creating a competitive inhibitor that can prevent the effector protein secreted by P. infestans from entering the potato cell. In case that the potatoes we genetically modified cannot fend off the P. infestans, we designed a soil based microbial fuel cell (SMFC) with engineered bacteria on the anode of the SMFC that can detect whether the potato tuber is infected or not. If the potato is infected and detected by our SMFC, we will spray defensin, an antimicrobial peptide isolated from maca (Lepidium meyenii). The defensin can weaken and inhibit the growth of P. infestans without doing harm to the environment and human body since it does not contain heavy metals like most fungicides.