Difference between revisions of "Team:Evry"

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     <!-- Main Content -->
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         <h1 class="text-center">Yeast cancer immunotherapy.</h1>
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       <div style="position: relative; top: 50%; transform: translateY(-50%);  -webkit-transform: translateY(-50%);">
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         <p class="text-center">EVRY-GENOPOLE IGEM 2015 PROJECT</p>
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        <p class="text-center" style="font-weight: 400; font-size:4em; color: #ffffff; text-shadow: 0px 0px 8px #222222;">Welcome on board!</p>
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         <p class="text-center" style="font-weight: 300; font-size:3em; color: #ffffff; text-shadow: 0px 0px 8px #222222;">iGEM Evry 2015</p>
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         <p class="text-center" style="font-weight: 200; font-size:2em; color: #ffffff; text-shadow: 0px 0px 8px #222222;">The YETI project to reshape the immune landscape</p>
 
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     <div class="page-header">
 
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     <h1>Welcome!</h1> <!--  to our project homepage -->
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     <h1>Let's begin with a small abstract</h1>
 
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<h2>Abstract</h2>
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<p class="lead">Reshaping immunotherapy landscape.</p>
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<p class="text-justify" style="font-size: 120%;">Cancer thrives by preventing the immune system from targeting tumor cells. While current immunotherapies use dendritic cells to activate T-cells towards specific tumor antigens, they remain expensive and of variable efficiency against tumor immunosuppressive environment. To address these issues, our team mainly focused on engineering a S. cerevisiae yeast immunotherapy that was ultimately tested <strong>in vivo</strong> on mice presenting melanoma.</p>
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<p class="text-justify" style="font-size: 120%;">Three complementary strategies were combined: First, in order to modulate the tumor environment, yeast secreting immune modulators, GM-CSF and IFNgamma, were encapsulated into alginate beads and injected in tumors. Secondly, to break the immune tolerance against cancer cells, T4 and T8 lymphocytes were elicited by a yeast antigen display system. Last, to deliver cytotoxic compounds solely in the tumor environment, a yeast hypoxia bio-sensor was designed. A side project consisted in engineering E. coli to drive MAIT lymphocytes against cancer cells instead of their original targets, parasitized cells.</p>
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Cancer thrives by preventing the <strong>immune system</strong>  from targeting tumor cells. While current immunotherapies use dendritic cells to activate T-cells towards specific tumor antigens, they remain expensive and of variable efficiency against tumor immunosuppressive environment. To develop <strong>personalized therapies</strong>, our team focused on engineering yeast <em>Saccharomyces cerevisiae</em> for targeted immunotherapy. First, we developed a <strong>software to select the best tumor antigen</strong> from patient sequencing data. Second, we created a <strong>yeast chassis</strong> to prime the immune system with the targeted antigen. This chassis was tested <em>in vitro</em> on mouse splenocytes and <em>in vivo</em> on mice presenting melanoma with significant results. Three complementary strategies were combined to induce the immune system. First, in order to modulate the <strong>tumor environment</strong>, yeast secreting the specific immune modulator IFNgamma was encapsulated into alginate beads to be injected in tumors. Secondly, to <strong>break the immune tolerance</strong> against cancer cells, T4 and T8 lymphocytes were elicited by a yeast antigen display system that can be adapted to any tumor antigen for personalized therapy. Last, to <strong>deliver cytotoxic compounds</strong> solely in the tumor environment, a yeast hypoxia bio-sensor was designed. Our <strong>standardized</strong> and <strong>customizable</strong> chassis takes advantage of these approaches to make personalized medicine a reality, with a scalable cancer therapy.</p>
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<!--p class="lead text-justify">Our immune system can be seen as a balance between immunity and tolerance.  
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This mechanism sometimes fails and lead to deleterious effects:  
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<section class="page-section">
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a too important immune response can lead to food allergies or Inflammatory Bowel Disease.
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<p class="text-justify lead">Read our <a href="https://2015.igem.org/Team:Evry/Description">project description</a> to learn more!</p>
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Absence of an immune response, in the other hand, plays a role in the spread of cancer cells.</p-->
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<!--p class="lead">Dendritic cells can orchestrate the immune response. By acting on them using engineered micro-organisms,
 
<!--p class="lead">Dendritic cells can orchestrate the immune response. By acting on them using engineered micro-organisms,
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Latest revision as of 20:09, 20 November 2015

   

Let's begin with a small abstract

Cancer thrives by preventing the immune system from targeting tumor cells. While current immunotherapies use dendritic cells to activate T-cells towards specific tumor antigens, they remain expensive and of variable efficiency against tumor immunosuppressive environment. To develop personalized therapies, our team focused on engineering yeast Saccharomyces cerevisiae for targeted immunotherapy. First, we developed a software to select the best tumor antigen from patient sequencing data. Second, we created a yeast chassis to prime the immune system with the targeted antigen. This chassis was tested in vitro on mouse splenocytes and in vivo on mice presenting melanoma with significant results. Three complementary strategies were combined to induce the immune system. First, in order to modulate the tumor environment, yeast secreting the specific immune modulator IFNgamma was encapsulated into alginate beads to be injected in tumors. Secondly, to break the immune tolerance against cancer cells, T4 and T8 lymphocytes were elicited by a yeast antigen display system that can be adapted to any tumor antigen for personalized therapy. Last, to deliver cytotoxic compounds solely in the tumor environment, a yeast hypoxia bio-sensor was designed. Our standardized and customizable chassis takes advantage of these approaches to make personalized medicine a reality, with a scalable cancer therapy.

Read our project description to learn more!

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