Difference between revisions of "Template:Team:Groningen/CONTENT/Home/Blogs"

 
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<div class="text">The world is changing and so are the energy needs of humanity. Fossil fuels aren’t endless and it is clear that a transition to clean sustainable energy has to be made. We believe synthetic biology can be an important catalyst in this process. Specifically, we engineer a Bacillus subtilis  biofilm to function as a cation exchange membrane. Such a membrane can be used in Reverse Electrodialysis (RED), a technique to generate energy where salt and fresh water mix, for example where rivers flow into the sea. We call this application of the bacterial biofilm  Blue Bio Energy. It is estimated that it can meet 4-7 % of the world energy demand. </div>
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Our biofilm was grow on a carrier material for strength and durability. Whatman paper was chosen for its great biofilm growth and low cost. Four growth methods were studied to optimize biofilm growth and strength.
 
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|title=Biofilm ion selectivity
 
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The J-PGA molecule was likely to improve the energy that could be generated with the test set up.. Therefore we made several models with Molecular Dynamics after this did tests in the lab with natto strain of <i>Bacillus subtilis</i>.
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The ion selectivity for Na+ and Cl- of a membrane of negatively charged &gamma;-PGA molecules was modelled using Molecular Dynamics. Wetlab testing was performed using <i>B. subtilis</i> Natto.
 
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|title=Rigidity of the biofilm
 
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Ut tortor urna, malesuada a nisi ac, suscipit sagittis lorem. Cras maximus, diam nec finibus venenatis, leo ante efficitur mauris, rhoncus condimentum metus ipsum et lectus. Quisque sapien felis, sodales a tempus sit amet, feugiat sit amet felis.
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To survive water flow, the biofilm has to be stable and robust. This was done by overexpressing genes involved in biofilm formation and by knocking out genes having the opposite effect.
 
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|title=Integrate bacteria into local environment
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|title=New shuttle vector
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Cras pellentesque rutrum est quis ultrices. Phasellus sodales eros in purus auctor eleifend. Proin sit amet odio non dolor commodo viverra. Nulla a urna finibus, posuere purus sed, posuere dui. In elit nunc, pretium non mi sit amet, blandit gravida nibh. Donec quis arcu dui. Sed aliquam fermentum nisl nec auctor. Integer felis magna, mollis eu facilisis non.
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An extra integration locus for <i>Bacillus</i>, such as the <i>thrC</i> locus, is welcome when making a multiple mutant. The <i>amyE</i> locus parts of the BBa_K823023 backbone were replaced with the <i>thrC</i> locus parts from the plasmid pDG1664, resulting in a new shuttle vector.
 
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|title=Self-sustainability
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|title=Human Practices
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Curabitur condimentum hendrerit eleifend. Sed fermentum ornare faucibus. Cras justo orci, malesuada in venenatis eget, pharetra a orci. Duis odio nunc, faucibus at pulvinar mattis, luctus vitae turpis. Phasellus id augue elementum eros sodales facilisis non quis elit.
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When working with GMOs, it is important to know the regulations and to think about the final application and the response of the public. To address this, we visited COGEM, designed an educational card game and considered several future scenarios involving GMOs and our project.
 
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|title=And Yes
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Donec ut interdum diam. Nullam malesuada erat vitae orci efficitur, ac finibus augue sollicitudin. Curabitur ornare augue at viverra commodo. Fusce efficitur neque dolor, nec rhoncus mauris mollis eget. Donec faucibus semper felis, quis feugiat dolor scelerisque sit amet. Morbi hendrerit sem quam, nec pharetra metus finibus vitae..
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Our bacteria need to stay and survive in the power plant. Ideally, the bacteria cannot escape, and the biofilm is sustained by using the nutrients present in water.
 
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Latest revision as of 22:34, 20 November 2015

<img class="image" src="Igem.groningen.2015.figure.small.front_carrier.png"/>

iGEM Groningen
00:00 00-00-2015
None
Carrier on which the biofilm grows
Our biofilm was grow on a carrier material for strength and durability. Whatman paper was chosen for its great biofilm growth and low cost. Four growth methods were studied to optimize biofilm growth and strength.

<img class="image" src="Igem.groningen.2015.figure.small.front_ionsel.png"/>

iGEM Groningen
00:00 00-00-2015
None
Biofilm ion selectivity
The ion selectivity for Na+ and Cl- of a membrane of negatively charged γ-PGA molecules was modelled using Molecular Dynamics. Wetlab testing was performed using B. subtilis Natto.

<img class="image" src="Igem.groningen.2015.figure.small.front_third.png"/>

iGEM Groningen
00:00 00-00-2015
None
Rigidity of the biofilm
To survive water flow, the biofilm has to be stable and robust. This was done by overexpressing genes involved in biofilm formation and by knocking out genes having the opposite effect.

<img class="image" src="Igem.groningen.2015.figure.small.front_fourth.png"/>

iGEM Groningen
00:00 00-00-2015
None
New shuttle vector
An extra integration locus for Bacillus, such as the thrC locus, is welcome when making a multiple mutant. The amyE locus parts of the BBa_K823023 backbone were replaced with the thrC locus parts from the plasmid pDG1664, resulting in a new shuttle vector.

<img class="image" src="Igem.groningen.2015.figure.small.front_notlast.png"/>

iGEM Groningen
00:00 00-00-2015
None
Human Practices
When working with GMOs, it is important to know the regulations and to think about the final application and the response of the public. To address this, we visited COGEM, designed an educational card game and considered several future scenarios involving GMOs and our project.

<img class="image" src="Igem.groningen.2015.figure.small.front_last.png"/>

iGEM Groningen
00:00 00-00-2015
None
Future perspective
Our bacteria need to stay and survive in the power plant. Ideally, the bacteria cannot escape, and the biofilm is sustained by using the nutrients present in water.