Difference between revisions of "Template:Team:Groningen/CONTENT/Home/Blogs"

 
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|title=Carrier on which the biofilm grows
 
|title=Carrier on which the biofilm grows
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The biofilm had to grow on a carrier material for strength and usability in the prototype. Whatman paper was chosen for its great biofilm growth and low cost. Four growth methods were studied to really optimize biofilm growth and strength.
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Our biofilm was grow on a carrier material for strength and durability. Whatman paper was chosen for its great biofilm growth and low cost. Four growth methods were studied to optimize biofilm growth and strength.
 
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|color=green
 
|color=green
 
|title=Biofilm ion selectivity
 
|title=Biofilm ion selectivity
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The J-PGA molecule was likely to improve the energy that could be generated with the test set up.. Therefore we made several models with Molecular Dynamics after this did tests in the lab with natto strain of <i>Bacillus subtilis</i>.
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The ion selectivity for Na+ and Cl- of a membrane of negatively charged &gamma;-PGA molecules was modelled using Molecular Dynamics. Wetlab testing was performed using <i>B. subtilis</i> Natto.
 
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|color=red
 
|color=red
 
|title=Rigidity of the biofilm
 
|title=Rigidity of the biofilm
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To survive the waterflow the biofilm had to be stable and robust. Biofilm genes have been overexpressed to be constantly transcribed. Furthermore stability has been improved by repressing and knocking out genetic pathways resulting in cell motility and biofilm repression.
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To survive water flow, the biofilm has to be stable and robust. This was done by overexpressing genes involved in biofilm formation and by knocking out genes having the opposite effect.
 
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|color=blue
 
|color=blue
 
|title=New shuttle vector
 
|title=New shuttle vector
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The BBa_K823023 backbone is one of the standard backbones for bacillus for integrating in the <i>amyE</i> locus. However an extra integration locus is welcome when making a multiple mutant. Another integration loci is the thrC locus. To create this backbone the BBa_K823023 backbone was used, where the amyE locus parts were replaced with the thrC locus parts from the plasmid pdg1664. This resulted in a new shuttle vector for <i>B. subtilis</i>.
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An extra integration locus for <i>Bacillus</i>, such as the <i>thrC</i> locus, is welcome when making a multiple mutant. The <i>amyE</i> locus parts of the BBa_K823023 backbone were replaced with the <i>thrC</i> locus parts from the plasmid pDG1664, resulting in a new shuttle vector.
 
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|title=Human practices
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|title=Human Practices
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When working with GMO’s it is important to know the regulations and think about future perspectives, which we did with possible future scenarios. Also it is important to talk about iGEM and your project at events, with several media, at this occasions we used our own card game.
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When working with GMOs, it is important to know the regulations and to think about the final application and the response of the public. To address this, we visited COGEM, designed an educational card game and considered several future scenarios involving GMOs and our project.
 
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|color=aqua
 
|color=aqua
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|title=Future perspective
 
|title=Future perspective
 
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To implement our project the GMOs need to stay in the power plant. Therefore we thought about capsulating the bacteria into two carriers, antimicrobial layers and a kill switch.The ultimate goal is that the biofilm can sustain itself from nutrients in the water.
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Our bacteria need to stay and survive in the power plant. Ideally, the bacteria cannot escape, and the biofilm is sustained by using the nutrients present in water.
 
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Latest revision as of 22:34, 20 November 2015

<img class="image" src="Igem.groningen.2015.figure.small.front_carrier.png"/>

iGEM Groningen
00:00 00-00-2015
None
Carrier on which the biofilm grows
Our biofilm was grow on a carrier material for strength and durability. Whatman paper was chosen for its great biofilm growth and low cost. Four growth methods were studied to optimize biofilm growth and strength.

<img class="image" src="Igem.groningen.2015.figure.small.front_ionsel.png"/>

iGEM Groningen
00:00 00-00-2015
None
Biofilm ion selectivity
The ion selectivity for Na+ and Cl- of a membrane of negatively charged γ-PGA molecules was modelled using Molecular Dynamics. Wetlab testing was performed using B. subtilis Natto.

<img class="image" src="Igem.groningen.2015.figure.small.front_third.png"/>

iGEM Groningen
00:00 00-00-2015
None
Rigidity of the biofilm
To survive water flow, the biofilm has to be stable and robust. This was done by overexpressing genes involved in biofilm formation and by knocking out genes having the opposite effect.

<img class="image" src="Igem.groningen.2015.figure.small.front_fourth.png"/>

iGEM Groningen
00:00 00-00-2015
None
New shuttle vector
An extra integration locus for Bacillus, such as the thrC locus, is welcome when making a multiple mutant. The amyE locus parts of the BBa_K823023 backbone were replaced with the thrC locus parts from the plasmid pDG1664, resulting in a new shuttle vector.

<img class="image" src="Igem.groningen.2015.figure.small.front_notlast.png"/>

iGEM Groningen
00:00 00-00-2015
None
Human Practices
When working with GMOs, it is important to know the regulations and to think about the final application and the response of the public. To address this, we visited COGEM, designed an educational card game and considered several future scenarios involving GMOs and our project.

<img class="image" src="Igem.groningen.2015.figure.small.front_last.png"/>

iGEM Groningen
00:00 00-00-2015
None
Future perspective
Our bacteria need to stay and survive in the power plant. Ideally, the bacteria cannot escape, and the biofilm is sustained by using the nutrients present in water.