Difference between revisions of "Team:DTU-Denmark"
| (5 intermediate revisions by 2 users not shown) | |||
| Line 3: | Line 3: | ||
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no"> | <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no"> | ||
<meta http-equiv="X-UA-Compatible" content="IE=edge"> | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | + | <link rel=stylesheet href="/Team:DTU-Denmark/loadphpcss?action=raw&ctype=text/css"> | |
| + | <link rel=stylesheet href="/Team:DTU-Denmark/bootstrapcss?action=raw&ctype=text/css"> | ||
| + | <link rel=stylesheet href="/Team:DTU-Denmark/font-awesomecss?action=raw&ctype=text/css"> | ||
| + | <link rel=stylesheet href="/Team:DTU-Denmark/wikicss?action=raw&ctype=text/css"> | ||
| + | <link rel=stylesheet href="/Team:DTU-Denmark/timelinecss?action=raw&ctype=text/css"> | ||
| + | <link rel=stylesheet href="/Team:DTU-Denmark/menufixcss?action=raw&ctype=text/css"> | ||
| + | |||
<script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script> | <script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script> | ||
<script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script> | <script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script> | ||
<script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script> | <script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script> | ||
<script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script> | <script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script> | ||
| + | |||
<!-- MathJax (LaTeX for the web) --> | <!-- MathJax (LaTeX for the web) --> | ||
<script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script> | <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</head> | </head> | ||
| Line 44: | Line 37: | ||
<i class="fa fa-bars"></i> | <i class="fa fa-bars"></i> | ||
</button> | </button> | ||
| − | <a class="navbar-brand navimg" href="#"><img src="/ | + | <a class="navbar-brand navimg" href="#"><img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png"></a> |
</div> | </div> | ||
<!-- Collect the nav links, forms, and other content for toggling --> | <!-- Collect the nav links, forms, and other content for toggling --> | ||
<div class="collapse navbar-collapse" id="nav-collapse"> | <div class="collapse navbar-collapse" id="nav-collapse"> | ||
<ul class="nav navbar-nav"> | <ul class="nav navbar-nav"> | ||
| − | + | <li class="active"> | |
| − | + | <a href="/Team:DTU-Denmark" | |
| − | + | >Home | |
| − | + | </a></li> | |
| − | + | <li | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | > | |
| − | + | <a href="" | |
| − | + | class="dropdown-toggle" | |
| − | + | data-toggle="dropdown" | |
| − | + | role="button" | |
| − | + | aria-expanded="false">Team and Attributions | |
| − | + | <span class="caret"></span></a><ul class="dropdown-menu" role="menu"> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Team" | |
| − | + | >Team | |
| − | + | </a></li> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Attributions" | |
| − | + | >Attributions | |
| − | + | </a></li></ul></li> | |
| − | + | <li | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| + | > | ||
| + | <a href="" | ||
| + | class="dropdown-toggle" | ||
| + | data-toggle="dropdown" | ||
| + | role="button" | ||
| + | aria-expanded="false">Project | ||
| + | <span class="caret"></span></a><ul class="dropdown-menu" role="menu"> | ||
| + | <li > | ||
| + | <a href="/Team:DTU-Denmark/Project/Overview" | ||
| + | >Overview | ||
| + | </a></li> | ||
| + | <li > | ||
| + | <a href="/Team:DTU-Denmark/Project/Background" | ||
| + | >Background | ||
| + | </a></li> | ||
| + | <li > | ||
| + | <a href="/Team:DTU-Denmark/Project/MAGE" | ||
| + | >MAGE subtilis | ||
| + | </a></li> | ||
| + | <li > | ||
| + | <a href="/Team:DTU-Denmark/Project/Tyrocidine" | ||
| + | >Tyrocidine | ||
| + | </a></li> | ||
| + | <li > | ||
| + | <a href="/Team:DTU-Denmark/Project/Chip" | ||
| + | >Lab-on-a-disc | ||
| + | </a></li> | ||
| + | <li > | ||
| + | <a href="/Team:DTU-Denmark/Project/Inteins" | ||
| + | >Inteins | ||
| + | </a></li> | ||
| + | <li > | ||
| + | <a href="/Team:DTU-Denmark/Project/Detection" | ||
| + | >Detection of NRP | ||
| + | </a></li></ul></li> | ||
| + | <li > | ||
| + | <a href="/Team:DTU-Denmark/Practices" | ||
| + | >Human Practices | ||
| + | </a></li> | ||
| + | <li | ||
| + | |||
| − | + | ||
| − | + | ||
| − | + | > | |
| − | + | <a href="" | |
| − | + | class="dropdown-toggle" | |
| − | + | data-toggle="dropdown" | |
| − | + | role="button" | |
| − | + | aria-expanded="false">Parts Collection | |
| − | + | <span class="caret"></span></a><ul class="dropdown-menu" role="menu"> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Parts" | |
| − | + | >Parts | |
| − | + | </a></li> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Description" | |
| − | + | >Characterisation of xylR | |
| − | + | </a></li></ul></li> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Journal" | |
| − | + | >Journal | |
| − | + | </a></li> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Software" | |
| − | + | >Software | |
| − | + | </a></li> | |
| − | + | <li | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | > | |
| − | + | <a href="" | |
| − | + | class="dropdown-toggle" | |
| − | + | data-toggle="dropdown" | |
| − | + | role="button" | |
| − | + | aria-expanded="false">Achievements | |
| − | + | <span class="caret"></span></a><ul class="dropdown-menu" role="menu"> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Achievements" | |
| − | + | >Key Achievements | |
| − | + | </a></li> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Collaborations" | |
| − | + | >Collaborations | |
| − | + | </a></li> | |
| − | + | <li > | |
| − | + | <a href="/Team:DTU-Denmark/Judging_Form" | |
| − | + | >Judging Form | |
| − | + | </a></li></ul></li> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<li class="hidden-xs"> | <li class="hidden-xs"> | ||
<a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook"> | <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook"> | ||
| Line 211: | Line 192: | ||
<li class="hidden-lg"> | <li class="hidden-lg"> | ||
<a href="https://igem.org" target="_blank" class="navimg"> | <a href="https://igem.org" target="_blank" class="navimg"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png"> |
</a> | </a> | ||
</li> | </li> | ||
| Line 218: | Line 199: | ||
</div> | </div> | ||
</nav> | </nav> | ||
| − | + | ||
| − | + | ||
| − | + | <nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix"> | |
| − | + | <div class="container-fluid" id="scrollspy"> | |
| − | + | <ul class="nav navbar-nav"> | |
| − | + | <li><a class="page-scroll" href="#ProjectDescription">Project Description</a></li><li><a class="page-scroll" href="#SocialMedia">Social Media</a></li> | |
| − | + | </ul> | |
| − | + | </div> | |
| + | </nav> | ||
| + | |||
| + | |||
| + | <div class="jumbotron hero" style="background-image: url(https://static.igem.org/mediawiki/2015/0/0b/DTU-Denmark_hero.jpg)"> | ||
<div class="igem-logo visible-lg"> | <div class="igem-logo visible-lg"> | ||
<a href="https://igem.org" target="_blank"> | <a href="https://igem.org" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 235: | Line 220: | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
| − | + | <h3>Technical University of Denmark</h3> | |
| − | + | <h1>The Synthesizer</h1> | |
| − | + | ||
<hr/> | <hr/> | ||
| + | |||
| + | |||
<ul class="list-inline hidden-xs hidden-sm"> | <ul class="list-inline hidden-xs hidden-sm"> | ||
<li> | <li> | ||
| − | <a id=" | + | <a id="ProjectDescription-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#ProjectDescription">Project Description</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a id=" | + | <a id="SocialMedia-submenu" class="btn btn-default btn-transparent btn-lg page-scroll" href="#SocialMedia">Social Media</a> |
</li> | </li> | ||
| Line 253: | Line 240: | ||
<li> | <li> | ||
| − | <a id=" | + | <a id="ProjectDescription-submenu" class="page-scroll" href="#ProjectDescription">Project Description</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a id=" | + | <a id="SocialMedia-submenu" class="page-scroll" href="#SocialMedia">Social Media</a> |
</li> | </li> | ||
</ul> | </ul> | ||
| + | |||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="row"> | <div class="row"> | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
Scroll down for more<br> | Scroll down for more<br> | ||
| − | <a href="# | + | <a href="#ProjectDescription" class="btn btn-circle btn-transparent page-scroll"> |
<i class="fa fa-angle-double-down"></i> | <i class="fa fa-angle-double-down"></i> | ||
</a> | </a> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
| − | + | ||
| − | <div id=" | + | |
| + | <div id="ProjectDescription"> | ||
<div class="container"> | <div class="container"> | ||
| + | |||
<div class="row col-md-12"> | <div class="row col-md-12"> | ||
<h1> | <h1> | ||
| − | Project | + | Project Description |
</h1> | </h1> | ||
| − | + | ||
| + | <p style="text-align: center;"><span style="font-size:72px;"><span style="font-size:72px;"><span class="fa fa-headphones" style="color:rgb(0, 0, 0);"></span></span></span></p> | ||
| − | <p style="text-align: center;"> | + | <p style="text-align: center;">Listen to our podcast about our <a href="https://static.igem.org/mediawiki/2015/5/50/Podcast_project.mp4"><em>Project</em></a> or <a href="https://static.igem.org/mediawiki/2015/f/f5/Podcast_education.mp4"><em>Human practises</em></a></p> |
| − | <p | + | <p> </p> |
| − | <p style=" | + | <p><img alt="" src="https://static.igem.org/mediawiki/2015/5/5e/DTU-Denmark_gold.png" style="float:left; margin-right:10px;" /></p> |
| − | <p | + | <p><br /> |
| + | DTU Denmark received a gold medal at the Giant Jamboree in Boston 2015 and a nomination for best educational human practises. Click on the headphones to hear our podcasts explaining our project and our human practises.</p> | ||
| + | |||
| + | <p>We also designed a <a href="https://2015.igem.org/Team:DTU-Denmark/Software">Wiki Wizard</a> that allows you to easily create and design wikis without knowing about HTML, CSS, and JavaScript. By using the shared editing mode, team members can edit the Wiki at the same time - just like Google Docs.</p> | ||
<p> </p> | <p> </p> | ||
| + | <p>Nonribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.</p> | ||
| + | |||
| + | <p style="text-align: center;"><img alt="" src="https://static.igem.org/mediawiki/2015/7/7d/DTU-Denmark_drawingpng.png" style="width: 625px; height: 378px;" /></p> | ||
| + | |||
| + | <p> </p> | ||
| + | |||
| + | |||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
| − | + | ||
| − | + | ||
| − | <div id=" | + | <div id="SocialMedia"> |
<div class="container"> | <div class="container"> | ||
| + | |||
<div class="row col-md-12"> | <div class="row col-md-12"> | ||
<h1> | <h1> | ||
Social Media | Social Media | ||
</h1> | </h1> | ||
| − | + | ||
| + | <div class="col-md-6"> | ||
<p><a class="twitter-timeline" data-widget-id="631150747221139456" href="https://twitter.com/iGEM_DTU">Tweets by @iGEM_DTU</a> <script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script></p> | <p><a class="twitter-timeline" data-widget-id="631150747221139456" href="https://twitter.com/iGEM_DTU">Tweets by @iGEM_DTU</a> <script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script></p> | ||
</div> | </div> | ||
| Line 316: | Line 323: | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
| − | + | ||
| − | < | + | |
| + | <footer class="container"> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-2 col-md-offset-2"> | <div class="col-md-2 col-md-offset-2"> | ||
<a href="http://www.dtu.dk" target="_blank"> | <a href="http://www.dtu.dk" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png" height="130px"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 340: | Line 350: | ||
<div class="col-md-2"> | <div class="col-md-2"> | ||
<a href="https://igem.org" style="float:left" target="_blank"> | <a href="https://igem.org" style="float:left" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png" height="90px"> |
</a> | </a> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | </ | + | </footer> |
<div class="sponsors"> | <div class="sponsors"> | ||
| − | <div class="container | + | <div class="container"> |
<div class="row"> | <div class="row"> | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="http://www.lundbeckfonden.com/" target="_blank"> | <a href="http://www.lundbeckfonden.com/" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/e/e2/DTU-Denmark_Lundbeckfonden.jpg"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 357: | Line 367: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="http://www.ottomoensted.dk" target="_blank"> | <a href="http://www.ottomoensted.dk" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/3/35/DTU-Denmark_Ottomoensted.png"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 363: | Line 373: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="http://www.novonordiskfonden.dk/en" target="_blank"> | <a href="http://www.novonordiskfonden.dk/en" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/5/5c/DTU-Denmark_NovoNordiskFonden_logo.png"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 369: | Line 379: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="https://dk.vwr.com/" target="_blank"> | <a href="https://dk.vwr.com/" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/e/ea/DTU-Denmark_VWR.jpg"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 375: | Line 385: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="http://frisenette.dk" target="_blank"> | <a href="http://frisenette.dk" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/c/c8/DTU-Denmark_Frisenette.jpg"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 381: | Line 391: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="http://www.in-vitro.dk/" target="_blank"> | <a href="http://www.in-vitro.dk/" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/7/7c/DTU-Denmark_In_Vitro.jpg"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 387: | Line 397: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="https://dk.fishersci.com/dk/" target="_blank"> | <a href="https://dk.fishersci.com/dk/" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/b/b4/DTU-Denmark_Fisher_Scientific.jpg"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 393: | Line 403: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="http://us.akg.com/akg-homepage-us.html" target="_blank"> | <a href="http://us.akg.com/akg-homepage-us.html" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/9/9d/DTU-Denmark_AKG.png"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 399: | Line 409: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="http://www.macrogen.com/" target="_blank"> | <a href="http://www.macrogen.com/" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/1/11/DTU-Denmark_Marcogen.jpg"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 405: | Line 415: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="http://www.snapgene.com/" target="_blank"> | <a href="http://www.snapgene.com/" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/5/58/DTU-Denmark_Snapgene.png"> |
</a> | </a> | ||
</div> | </div> | ||
| Line 411: | Line 421: | ||
<div class="sponsorlogo"> | <div class="sponsorlogo"> | ||
<a href="https://www.neb.com/" target="_blank"> | <a href="https://www.neb.com/" target="_blank"> | ||
| − | <img src="/ | + | <img src="https://static.igem.org/mediawiki/2015/e/e0/DTU-Denmark_NEB_logo.png"> |
</a> | </a> | ||
</div> | </div> | ||
Latest revision as of 23:51, 20 November 2015
Project Description
Listen to our podcast about our Project or Human practises
DTU Denmark received a gold medal at the Giant Jamboree in Boston 2015 and a nomination for best educational human practises. Click on the headphones to hear our podcasts explaining our project and our human practises.
We also designed a Wiki Wizard that allows you to easily create and design wikis without knowing about HTML, CSS, and JavaScript. By using the shared editing mode, team members can edit the Wiki at the same time - just like Google Docs.
Nonribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.
