Difference between revisions of "Template:Team:Groningen/CONTENT/PAE/treeall"

Line 1: Line 1:
 
[
 
[
 
{
 
{
"date":"28-06-2015",
+
"date":"05-09-2015",
"title":"Psalt PCR",  
+
"title":"tasA Restriction",  
"category":"zp",
+
"category":"tb",
 
"number": 1,
 
"number": 1,
 
"isroot": true,
 
"isroot": true,
"next": ["zp2"]
+
"next": ["tb3"]
 
},
 
},
 
{
 
{
"date":"29-06-2015",
+
"date":"05-09-2015",
"title":"Psalt Digestion",  
+
"title":"tasA bslA ligation",  
"category":"zp",
+
"category":"tb",
 +
"number": 3,
 +
"isroot": false,
 +
"next": ["tb4"]
 +
},
 +
{
 +
"date":"05-09-2015",
 +
"title":"bslA Restriction",
 +
"category":"tb",
 
"number": 2,
 
"number": 2,
 +
"isroot": true,
 +
"next": ["tb3"]
 +
},
 +
{
 +
"date":"05-09-2015",
 +
"title":"tasA bslA Transformation",
 +
"category":"tb",
 +
"number": 4,
 
"isroot": false,
 
"isroot": false,
"next": ["zp3"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"29-06-2015",
+
"date":"06-09-2015",
"title":"Psalt Ligation",  
+
"title":"tasA Restriction",
"category":"zp",
+
"category":"tb",
 +
"number": 5,
 +
"isroot": true,
 +
"next": ["tb7"]
 +
},
 +
{
 +
"date":"06-09-2015",
 +
"title":"tasA bslA ligation",
 +
"category":"tb",
 +
"number": 7,
 +
"isroot": false,
 +
"next": ["tb8"]
 +
},
 +
{
 +
"date":"06-09-2015",
 +
"title":"bslA Restriction",
 +
"category":"tb",
 +
"number": 6,
 +
"isroot": true,
 +
"next": ["tb8"]
 +
},
 +
{
 +
"date":"06-09-2015",
 +
"title":"tasA bslA Transformation",
 +
"category":"tb",
 +
"number": 8,
 +
"isroot": false,
 +
"next": ["tb9"]
 +
},
 +
{
 +
"date":"07-09-2015",
 +
"title":"tasA bslA Colony PCR",
 +
"category":"tb",
 +
"number": 9,
 +
"isroot": false,
 +
"next": ["tb10"]
 +
},
 +
{
 +
"date":"08-09-2015",
 +
"title":"tasA bslA Miniprep",
 +
"category":"tb",
 +
"number": 10,
 +
"isroot": false,
 +
"next": ["tb11"]
 +
},
 +
{
 +
"date":"08-09-2015",
 +
"title":"tasA bslA Transformation",
 +
"category":"tb",
 +
"number": 11,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"22-07-2015",
 +
"title":"Liquid Medium",
 +
"category":"co",
 +
"number": 1,
 +
"isroot": true,
 +
"next": ["co2","co3"]
 +
},
 +
{
 +
"date":"22-07-2015",
 +
"title":"CFR",
 +
"category":"co",
 +
"number": 2,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"24-07-2015",
 +
"title":"Centrifugation",  
 +
"category":"co",
 
"number": 3,
 
"number": 3,
 
"isroot": false,
 
"isroot": false,
"next": ["zp4"]
+
"next": ["co4"]
 
},
 
},
 
{
 
{
"date":"29-06-2015",
+
"date":"24-07-2015",
"title":"Psalt Transformation",  
+
"title":"Dissolve in PBS",  
"category":"zp",
+
"category":"co",
 
"number": 4,
 
"number": 4,
 +
"isroot": false,
 +
"next": ["co5"]
 +
},
 +
{
 +
"date":"24-07-2015",
 +
"title":"CFR",
 +
"category":"co",
 +
"number": 5,
 
"isroot": false,
 
"isroot": false,
 
"next": null
 
"next": null
 
},
 
},
 
{
 
{
"date":"30-06-2015",
+
"date":"20-07-2015",
"title":"Psalt PCR",  
+
"title":"CFR",
"category":"zp",
+
"category":"Liquid Medium",
 +
"number": 7,
 +
"isroot": true,
 +
"next": ["co8"]
 +
},
 +
{
 +
"date":"21-07-2015",
 +
"title":"Plate",
 +
"category":"co",
 +
"number": 8,
 +
"isroot": false,
 +
"next": ["co9"]
 +
},
 +
{
 +
"date":"22-07-2015",
 +
"title":"DFR",
 +
"category":"co",
 +
"number": 9,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"23-07-2015",
 +
"title":"Liquid Medium",
 +
"category":"co",
 +
"number": 10,
 +
"isroot": true,
 +
"next": ["co11"]
 +
},
 +
{
 +
"date":"24-07-2015",
 +
"title":"Plate",
 +
"category":"co",
 +
"number": 11,
 +
"isroot": false,
 +
"next": ["co12"]
 +
},
 +
{
 +
"date":"25-07-2015",
 +
"title":"DFR",
 +
"category":"co",
 +
"number": 12,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"29-07-2015",
 +
"title":"Liquid Medium",
 +
"category":"co",
 +
"number": 15,
 +
"isroot": true,
 +
"next": ["co16","co17"]
 +
},
 +
{
 +
"date":"30-07-2015",
 +
"title":"Plate",
 +
"category":"co",
 +
"number": 16,
 +
"isroot": false,
 +
"next": ["co17"]
 +
},
 +
{
 +
"date":"30-07-2015",
 +
"title":"Liquid Medium",
 +
"category":"co",
 +
"number": 17,
 +
"isroot": false,
 +
"next": ["co21"]
 +
},
 +
{
 +
"date":"01-08-2015",
 +
"title":"Liquid Medium",
 +
"category":"co",
 +
"number": 18,
 +
"isroot": true,
 +
"next": ["co19"]
 +
},
 +
{
 +
"date":"03-08-2015",
 +
"title":"Plated",
 +
"category":"co",
 +
"number": 19,
 +
"isroot": false,
 +
"next": ["co20"]
 +
},
 +
{
 +
"date":"05-08-2015",
 +
"title":"DFR",
 +
"category":"co",
 +
"number": 20,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"05-08-2015",
 +
"title":"Plates",
 +
"category":"co",
 +
"number": 21,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"15-08-2015",
 +
"title":"Transformation with RFP",
 +
"category":"co",
 +
"number": 22,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"04-08-2015",
 +
"title":"Transformation with GFP",
 +
"category":"co",
 +
"number": 23,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"22-08-2015",
 +
"title":"Transformation with GFP",
 +
"category":"co",
 +
"number": 24,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"15-08-2015",
 +
"title":"TSB 2.0 vs TSBstar",
 +
"category":"ab",
 +
"number": 1,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"09-09-2015",
 +
"title":"Transform comI",
 +
"category":"ab",
 +
"number": 2,
 +
"isroot": true,
 +
"next": ["ab3"]
 +
},
 +
{
 +
"date":"11-09-2015",
 +
"title":"Transform with slrR",
 +
"category":"ab",
 +
"number": 3,
 +
"isroot": false,
 +
"next": ["ab4"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"Streak Plates",
 +
"category":"ab",
 +
"number": 4,
 +
"isroot": false,
 +
"next": ["ab5","ab6","ab7"]
 +
},
 +
{
 +
"date":"01-09-2015",
 +
"title":"New Streak Plates",  
 +
"category":"ab",
 
"number": 5,
 
"number": 5,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"30-08-2015",
 +
"title":"New Streak Plates",
 +
"category":"ab",
 +
"number": 6,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"03-09-2015",
 +
"title":"JH642 Streak Plate",
 +
"category":"ab",
 +
"number": 7,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"Transform Natto",
 +
"category":"ab",
 +
"number": 8,
 
"isroot": true,
 
"isroot": true,
"next":["zp6","zp10","zp14","zp21","zp24"]
+
"next": []
 
},
 
},
 
{
 
{
"date":"09-07-2015",
+
"date":"14-08-2015",
"title":"Psalt Digestion",  
+
"title":"Transform JH642, PY79",
"category":"zp",
+
"category":"ab",
 +
"number": 9,
 +
"isroot": false,
 +
"next": ["ab4"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Transform JH642, PY79, comI",
 +
"category":"ab",
 +
"number": 10,
 +
"isroot": false,
 +
"next": ["ab4"]
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"Transform JH642, PY79",
 +
"category":"ab",
 +
"number": 11,
 +
"isroot": false,
 +
"next": ["ab4"]
 +
},
 +
{
 +
"date":"14-08-2015",
 +
"title":"Transform JH642, PY79",
 +
"category":"ab",
 +
"number": 12,
 +
"isroot": false,
 +
"next": ["ab4"]
 +
},
 +
{
 +
"date":"28-08-2015",
 +
"title":"PCR",
 +
"category":"ab",
 +
"number": 13,
 +
"isroot": true,
 +
"next": ["ab14"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"A2 Recombination",
 +
"category":"ab",
 +
"number": 14,
 +
"isroot": false,
 +
"next": ["ab15"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"Restriction",
 +
"category":"ab",
 +
"number": 15,
 +
"isroot": false,
 +
"next": ["ab16"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"Ligation",
 +
"category":"ab",
 +
"number": 16,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"28-08-2015",
 +
"title":"PCR",
 +
"category":"a",
 +
"number": 17,
 +
"isroot": false,
 +
"next": ["a18"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"A1 Recombination",
 +
"category":"a",
 +
"number": 18,
 +
"isroot": false,
 +
"next": ["a19"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"Restriction",
 +
"category":"a",
 +
"number": 19,
 +
"isroot": false,
 +
"next": ["a20"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"Ligation",
 +
"category":"a",
 +
"number": 20,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"03-09-2015",
 +
"title":"PCR",
 +
"category":"a",
 +
"number": 21,
 +
"isroot": false,
 +
"next": ["a23"]
 +
},
 +
{
 +
"date":"05-09-2015",
 +
"title":"Digestion",  
 +
"category":"a",
 +
"number": 23,
 +
"isroot": false,
 +
"next": ["a24"]
 +
},
 +
{
 +
"date":"05-09-2015",
 +
"title":"Ligation",
 +
"category":"a",
 +
"number": 24,
 +
"isroot": false,
 +
"next": ["a25"]
 +
},
 +
{
 +
"date":"06-09-2015",
 +
"title":"Transform E. coli",
 +
"category":"a",
 +
"number": 25,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"02-09-2015",
 +
"title":"PCR",
 +
"category":"a",
 +
"number": 26,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"26-07-2015",
 +
"title":"Transformation",
 +
"category":"a",
 +
"number": 27,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"Rigidity OD",
 +
"category":"a",
 +
"number": 29,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"08-08-2015",
 +
"title":"Transformation",
 +
"category":"a",
 +
"number": 30,
 +
"isroot": false,
 +
"next": ["a29","a32","a34","a35","a39","a40","a41"]
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"OCV",
 +
"category":"a",
 +
"number": 31,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"15-08-2015",
 +
"title":"Transformation",
 +
"category":"a",
 +
"number": 32,
 +
"isroot": false,
 +
"next": ["a31","a33"]
 +
},
 +
{
 +
"date":"16-08-2015",
 +
"title":"OCV",
 +
"category":"a",
 +
"number": 33,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"25-07-2015",
 +
"title":"Rigidity cfu",
 +
"category":"a",
 +
"number": 34,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"24-07-2015",
 +
"title":"Rigidity OD",
 +
"category":"a",
 +
"number": 35,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"25-07-2015",
 +
"title":"bd1807 Purification",
 +
"category":"a",
 +
"number": 36,
 +
"isroot": true,
 +
"next": ["a27","a30","a26","a17","a21","a41"]
 +
},
 +
{
 +
"date":"10-08-2015",
 +
"title":"128 Submerged Plates",
 +
"category":"a",
 +
"number": 37,
 +
"isroot": true,
 +
"next": []
 +
},
 +
{
 +
"date":"20-08-2015",
 +
"title":"abrB Submerged Plates",
 +
"category":"a",
 +
"number": 38,
 +
"isroot": true,
 +
"next": []
 +
},
 +
{
 +
"date":"30-08-2015",
 +
"title":"30 vs 37",
 +
"category":"a",
 +
"number": 39,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"31-08-2015",
 +
"title":"30 vs 37",
 +
"category":"a",
 +
"number": 40,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"08-09-2015",
 +
"title":"PCR",
 +
"category":"a",
 +
"number": 41,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"16-04-2015",
 +
"title":"Primer Design",
 +
"category":"b",
 +
"number": 1,
 +
"isroot": true,
 +
"next": ["b2","b3","b4"]
 +
},
 +
{
 +
"date":"15-08-2015",
 +
"title":"PCR II",
 +
"category":"b",
 +
"number": 2,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"13-05-2015",
 +
"title":"PCR I",
 +
"category":"b",
 +
"number": 3,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"17-05-2015",
 +
"title":"PCR III",
 +
"category":"b",
 +
"number": 4,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"21-05-2015",
 +
"title":"New Primers",
 +
"category":"b",
 +
"number": 5,
 +
"isroot": true,
 +
"next": ["b6"]
 +
},
 +
{
 +
"date":"08-06-2015",
 +
"title":"PCR 168",
 +
"category":"b",
 
"number": 6,
 
"number": 6,
 
"isroot": false,
 
"isroot": false,
"next": ["zp7"]
+
"next": ["b7"]
 
},
 
},
 
{
 
{
"date":"09-07-2015",
+
"date":"08-06-2015",
"title":"Psalt PCR purification",  
+
"title":"Purification",
"category":"zp",
+
"category":"b",
 
"number": 7,
 
"number": 7,
 
"isroot": false,
 
"isroot": false,
"next": ["zp8"]
+
"next": ["b8"]
 
},
 
},
 
{
 
{
"date":"09-07-2015",
+
"date":"09-06-2015",
"title":"Psalt Ligation",  
+
"title":"Assembly PCR",
"category":"zp",
+
"category":"b",
 
"number": 8,
 
"number": 8,
 
"isroot": false,
 
"isroot": false,
"next": ["zp9"]
+
"next": ["b9"]
 
},
 
},
 
{
 
{
"date":"09-07-2015",
+
"date":"09-06-2015",
"title":"Psalt Transformation",  
+
"title":"Purification",
"category":"zp",
+
"category":"b",
 
"number": 9,
 
"number": 9,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b10"]
 
},
 
},
 
{
 
{
"date":"10-07-2015",
+
"date":"10-6-2015",
"title":"Psalt Digestion",  
+
"title":"Digestion",
"category":"zp",
+
"category":"b",
 
"number": 10,
 
"number": 10,
 
"isroot": false,
 
"isroot": false,
"next": ["zp11"]
+
"next": ["b11"]
 
},
 
},
 
{
 
{
"date":"10-07-2015",
+
"date":"10-06-2015",
"title":"Psalt PCR purification",  
+
"title":"PCR Purification",
"category":"zp",
+
"category":"b",
 
"number": 11,
 
"number": 11,
 
"isroot": false,
 
"isroot": false,
"next": ["zp12"]
+
"next": ["b12","b26","b23"]
 
},
 
},
 
{
 
{
"date":"10-07-2015",
+
"date":"16-07-2015",
"title":"Psalt Ligation",  
+
"title":"Ligation with Psalt",
"category":"zp",
+
"category":"b",
 
"number": 12,
 
"number": 12,
 
"isroot": false,
 
"isroot": false,
"next": ["zp13"]
+
"next": ["b13"]
 
},
 
},
 
{
 
{
"date":"10-07-2015",
+
"date":"16-07-2015",
"title":"Psalt Transformation",  
+
"title":"PCR Purification",
"category":"zp",
+
"category":"b",
 
"number": 13,
 
"number": 13,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b14"]
 
},
 
},
 
{
 
{
"date":"13-07-2015",
+
"date":"16-07-2015",
"title":"Psalt Digestion",  
+
"title":"Ligation with Psalt",
"category":"zp",
+
"category":"b",
 
"number": 14,
 
"number": 14,
 
"isroot": false,
 
"isroot": false,
"next": ["zp15"]
+
"next": ["b15"]
 
},
 
},
 
{
 
{
"date":"13-07-2015",
+
"date":"16-07-2015",
"title":"Psalt PCR purification",  
+
"title":"PCR Purification",
"category":"zp",
+
"category":"b",
 
"number": 15,
 
"number": 15,
 
"isroot": false,
 
"isroot": false,
"next": ["zp16"]
+
"next": ["b16"]
 
},
 
},
 
{
 
{
"date":"13-07-2015",
+
"date":"17-07-2015",
"title":"Psalt Ligation",  
+
"title":"Transformation",
"category":"zp",
+
"category":"b",
 
"number": 16,
 
"number": 16,
 
"isroot": false,
 
"isroot": false,
"next": ["zp17"]
+
"next": ["b17"]
 
},
 
},
 
{
 
{
"date":"13-07-2015",
+
"date":"17-07-2015",
"title":"Psalt Transformation",  
+
"title":"Colony PCR",
"category":"zp",
+
"category":"b",
 
"number": 17,
 
"number": 17,
 
"isroot": false,
 
"isroot": false,
"next": ["zp18","zp19","zp20"]
+
"next": ["b18"]
 
},
 
},
 
{
 
{
"date":"15-07-2015",
+
"date":"17-07-2015",
"title":"Psalt Colony PCR",  
+
"title":"Liquid Culture",
"category":"zp",
+
"category":"b",
 
"number": 18,
 
"number": 18,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b19"]
 
},
 
},
 
{
 
{
"date":"16-07-2015",
+
"date":"18-08-2015",
"title":"Psalt Colony PCR",  
+
"title":"Miniprep",
"category":"zp",
+
"category":"b",
 
"number": 19,
 
"number": 19,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b20","b21"]
 
},
 
},
 
{
 
{
"date":"17-07-2015",
+
"date":"20-07-2015",
"title":"Psalt Colony PCR",  
+
"title":"Sequencing",
"category":"zp",
+
"category":"b",
 
"number": 20,
 
"number": 20,
 
"isroot": false,
 
"isroot": false,
Line 161: Line 729:
 
},
 
},
 
{
 
{
"date":"20-07-2015",
+
"date":"21-07-2015",
"title":"Psalt Digestion",  
+
"title":"Transformation",
"category":"zp",
+
"category":"b",
 
"number": 21,
 
"number": 21,
 
"isroot": false,
 
"isroot": false,
"next": ["zp22"]
+
"next": []
 
},
 
},
 
{
 
{
"date":"20-07-2015",
+
"date":"23-07-2015",
"title":"Psalt Ligation",  
+
"title":"Digest with Psalt k23",
"category":"zp",
+
"category":"b",
 
"number": 22,
 
"number": 22,
 
"isroot": false,
 
"isroot": false,
"next": ["zp23"]
+
"next": ["b23"]
 
},
 
},
 
{
 
{
"date":"20-07-2015",
+
"date":"23-07-2015",
"title":"Psalt Transformation",  
+
"title":"PCR Purification",
"category":"zp",
+
"category":"b",
 
"number": 23,
 
"number": 23,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b24"]
 
},
 
},
 
{
 
{
"date":"21-07-2015",
+
"date":"23-07-2015",
"title":"Psalt Digestion",  
+
"title":"Ligation",
"category":"zp",
+
"category":"b",
 
"number": 24,
 
"number": 24,
 
"isroot": false,
 
"isroot": false,
"next": ["zp25"]
+
"next": ["b25"]
 
},
 
},
 
{
 
{
"date":"21-07-2015",
+
"date":"23-07-2015",
"title":"Psalt Purification",  
+
"title":"Plate",
"category":"zp",
+
"category":"b",
 
"number": 25,
 
"number": 25,
 
"isroot": false,
 
"isroot": false,
"next": ["zp26"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"21-07-2015",
+
"date":"10-6-2015",
"title":"Psalt Ligation",  
+
"title":"Ligation k23",
"category":"zp",
+
"category":"b",
 
"number": 26,
 
"number": 26,
 
"isroot": false,
 
"isroot": false,
"next": ["zp27"]
+
"next": ["b27"]
 
},
 
},
 
{
 
{
"date":"21-07-2015",
+
"date":"10-06-2015",
"title":"Psalt Transformation",  
+
"title":"Transformation E. coli",
"category":"zp",
+
"category":"b",
 
"number": 27,
 
"number": 27,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b28"]
 
},
 
},
 
{
 
{
"date":"24-07-2015",
+
"date":"11-06-2015",
"title":"Psalt New Primers",  
+
"title":"Liquid Medium",
"category":"zp",
+
"category":"b",
 
"number": 28,
 
"number": 28,
"isroot": true,
+
"isroot": false,
"next": ["zp29"]
+
"next": ["b29"]
 
},
 
},
 
{
 
{
"date":"16-08-2015",
+
"date":"12-06-2015",
"title":"Psalt PCR",  
+
"title":"Miniprep",
"category":"zp",
+
"category":"b",
 
"number": 29,
 
"number": 29,
 
"isroot": false,
 
"isroot": false,
"next": ["zp30"]
+
"next": ["b30","b31"]
 
},
 
},
 
{
 
{
"date":"17-08-2015",
+
"date":"15-06-2015",
"title":"Psalt Digestion",  
+
"title":"Digestion",
"category":"zp",
+
"category":"b",
 
"number": 30,
 
"number": 30,
 
"isroot": false,
 
"isroot": false,
"next": ["zp31"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"17-08-2015",
+
"date":"16-06-2015",
"title":"Psalt Purification",  
+
"title":"Digestion",
"category":"zp",
+
"category":"b",
 
"number": 31,
 
"number": 31,
 
"isroot": false,
 
"isroot": false,
"next": ["zp32"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"17-08-2015",
+
"date":"08-07-2015",
"title":"Psalt Ligation",  
+
"title":"Digestion psBlc3",
"category":"zp",
+
"category":"b",
 
"number": 32,
 
"number": 32,
"isroot": false,
+
"isroot": true,
"next": ["zp33"]
+
"next": ["b33"]
 
},
 
},
 
{
 
{
"date":"17-08-2015",
+
"date":"08-07-2015",
"title":"Psalt Transformation",  
+
"title":"PCR Purification",
"category":"zp",
+
"category":"b",
 
"number": 33,
 
"number": 33,
 
"isroot": false,
 
"isroot": false,
"next": ["zp34"]
+
"next": ["b34"]
 
},
 
},
 
{
 
{
"date":"18-08-2015",
+
"date":"08-07-2015",
"title":"Psalt Colony PCR",  
+
"title":"Ligation",
"category":"zp",
+
"category":"b",
 
"number": 34,
 
"number": 34,
 
"isroot": false,
 
"isroot": false,
"next": ["zp35"]
+
"next": ["b35"]
 
},
 
},
 
{
 
{
"date":"19-08-2015",
+
"date":"09-07-2015",
"title":"Psalt Miniprep",  
+
"title":"Transformation",
"category":"zp",
+
"category":"b",
 
"number": 35,
 
"number": 35,
 
"isroot": false,
 
"isroot": false,
"next": ["zp36","zp53","zp62"]
+
"next": ["b36"]
 
},
 
},
 
{
 
{
"date":"19-08-2015",
+
"date":"09-07-2015",
"title":"Psalt Digestion",  
+
"title":"Liquid Medium",
"category":"zp",
+
"category":"b",
 
"number": 36,
 
"number": 36,
 
"isroot": false,
 
"isroot": false,
"next": ["zp37"]
+
"next": ["b37"]
 
},
 
},
 
{
 
{
"date":"19-08-2015",
+
"date":"10-07-2015",
"title":"Psalt Purification",  
+
"title":"Miniprep",
"category":"zp",
+
"category":"b",
 
"number": 37,
 
"number": 37,
 
"isroot": false,
 
"isroot": false,
"next": ["zp38","zp46","zp48"]
+
"next": ["b67","b38","b46","b52","b54","b66","b55","b56"]
 
},
 
},
 
{
 
{
"date":"20-08-2015",
+
"date":"06-08-2015",
"title":"Psalt Ligation",  
+
"title":"Digestion",
"category":"zp",
+
"category":"b",
 
"number": 38,
 
"number": 38,
 
"isroot": false,
 
"isroot": false,
"next": ["zp101"]
+
"next": ["b39"]
 
},
 
},
 
{
 
{
"date":"20-09-2015",
+
"date":"06-08-2015",
"title":"Psalt Transformation",  
+
"title":"PCR Purification",
"category":"zp",
+
"category":"b",
"number": 101,
+
"number": 39,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b40"]
 
},
 
},
 
{
 
{
"date":"17-08-2015",
+
"date":"06-08-2015",
"title":"GFP Digestion",
+
"title":"Ligation",
"category":"zp",
+
"category":"b",
"number": 39,
+
"isroot": true,
+
"next": ["zp40"]
+
},
+
{
+
"date":"17-08-2015",
+
"title":"GFP Ligation",  
+
"category":"zp",
+
 
"number": 40,
 
"number": 40,
 
"isroot": false,
 
"isroot": false,
"next": ["zp41"]
+
"next": ["b41"]
 
},
 
},
 
{
 
{
"date":"17-08-2015",
+
"date":"07-08-2015",
"title":"GFP Transformation",  
+
"title":"Transformation",
"category":"zp",
+
"category":"b",
 
"number": 41,
 
"number": 41,
 
"isroot": false,
 
"isroot": false,
"next": ["zp42"]
+
"next": ["b42"]
 
},
 
},
 
{
 
{
"date":"18-08-2015",
+
"date":"09-08-2015",
"title":"GFP Colony PCR",  
+
"title":"Colony PCR",
"category":"zp",
+
"category":"b",
 
"number": 42,
 
"number": 42,
 
"isroot": false,
 
"isroot": false,
"next": ["zp43"]
+
"next": ["b43"]
 
},
 
},
 
{
 
{
"date":"19-08-2015",
+
"date":"09-08-2015",
"title":"GFP Miniprep",  
+
"title":"Overnight Culture",
"category":"zp",
+
"category":"b",
 
"number": 43,
 
"number": 43,
 
"isroot": false,
 
"isroot": false,
"next": ["zp44","zp50"]
+
"next": ["b44"]
 
},
 
},
 
{
 
{
"date":"19-08-2015",
+
"date":"11-08-2015",
"title":"GFP Digestion",  
+
"title":"Miniprep",
"category":"zp",
+
"category":"b",
 
"number": 44,
 
"number": 44,
 
"isroot": false,
 
"isroot": false,
"next": ["zp38"]
+
"next": ["b45"]
 
},
 
},
 
{
 
{
"date":"24-08-2015",
+
"date":"13-08-2015",
"title":"Psalt Digestion GFP",  
+
"title":"Check concentrations",
"category":"zp",
+
"category":"b",
"number": 50,
+
"number": 45,
 
"isroot": false,
 
"isroot": false,
"next": ["zp51"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"24-08-2015",
+
"date":"28-07-2015",
"title":"Psalt GFP Purification",  
+
"title":"Psalt Digestion",
"category":"zp",
+
"category":"b",
"number": 51,
+
"number": 46,
 
"isroot": false,
 
"isroot": false,
"next": ["zp60","zp103"]
+
"next": ["b47","b49"]
 
},
 
},
 
{
 
{
"date":"20-08-2015",
+
"date":"28-07-2015",
"title":"Psalt Ligation tasA",  
+
"title":"Ligation",
"category":"zp",
+
"category":"b",
 +
"number": 47,
 +
"isroot": false,
 +
"next": ["b48"]
 +
},
 +
{
 +
"date":"29-07-2015",
 +
"title":"Transformation",
 +
"category":"b",
 
"number": 48,
 
"number": 48,
 
"isroot": false,
 
"isroot": false,
"next": ["zp49"]
+
"next": []
 
},
 
},
 
{
 
{
"date":"20-08-2015",
+
"date":"29-07-2015",
"title":"Psalt tasA Transformation",  
+
"title":"PCR Purification",
"category":"zp",
+
"category":"b",
 
"number": 49,
 
"number": 49,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b50"]
 
},
 
},
 
{
 
{
"date":"20-08-2015",
+
"date":"28-07-2015",
"title":"Psalt Ligation bslA",  
+
"title":"Ligation",
"category":"zp",
+
"category":"b",
"number": 46,
+
"number": 50,
 
"isroot": false,
 
"isroot": false,
"next": ["zp47"]
+
"next": ["b51"]
 +
},
 +
{
 +
"date":"29-07-2015",
 +
"title":"Transformation",
 +
"category":"b",
 +
"number": 51,
 +
"isroot": false,
 +
"next": []
 
},
 
},
 
{
 
{
 
"date":"20-08-2015",
 
"date":"20-08-2015",
"title":"Psalt bslA Transformation",  
+
"title":"Ligation",
"category":"zp",
+
"category":"b",
"number": 47,
+
"number": 52,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["b53"]
 
},
 
},
 
{
 
{
"date":"24-08-2015",
+
"date":"21-08-2015",
"title":"Psalt Digestion",  
+
"title":"Colony PCR",
"category":"zp",
+
"category":"b",
 
"number": 53,
 
"number": 53,
 
"isroot": false,
 
"isroot": false,
"next": ["zp60","zp54","zp57"]
+
"next": null
 +
},
 +
{
 +
"date":"30-07-2015",
 +
"title":"Sequencing",
 +
"category":"b",
 +
"number": 54,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"30-08-2015",
 +
"title":"Transformation",
 +
"category":"b",
 +
"number": 55,
 +
"isroot": false,
 +
"next": []
 
},
 
},
 
{
 
{
 
"date":"24-08-2015",
 
"date":"24-08-2015",
"title":"Psalt Ligation bslA",  
+
"title":"Digestion Psalt",
"category":"zp",
+
"category":"b",
 +
"number": 56,
 +
"isroot": false,
 +
"next": ["b57"]
 +
},
 +
{
 +
"date":"24-8-2015",
 +
"title":"PCR Purification",
 +
"category":"b",
 
"number": 57,
 
"number": 57,
 
"isroot": false,
 
"isroot": false,
"next": ["zp58"]
+
"next": ["b58"]
 
},
 
},
 
{
 
{
 
"date":"24-08-2015",
 
"date":"24-08-2015",
"title":"Psalt bslA Transformation",  
+
"title":"Ligation",
"category":"zp",
+
"category":"b",
 
"number": 58,
 
"number": 58,
 
"isroot": false,
 
"isroot": false,
"next": ["zp59"]
+
"next": ["b59"]
 
},
 
},
 
{
 
{
"date":"25-08-2015",
+
"date":"24-08-2015",
"title":"Psalt bslA Miniprep",  
+
"title":"Transformation",
"category":"zp",
+
"category":"b",
 
"number": 59,
 
"number": 59,
 +
"isroot": false,
 +
"next": ["b60"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Miniprep",
 +
"category":"b",
 +
"number": 60,
 +
"isroot": false,
 +
"next": ["b61"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Digest k23",
 +
"category":"b",
 +
"number": 61,
 +
"isroot": false,
 +
"next": ["b62"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Ligation",
 +
"category":"b",
 +
"number": 62,
 +
"isroot": false,
 +
"next": ["b63"]
 +
},
 +
{
 +
"date":"27-08-2015",
 +
"title":"PCR I",
 +
"category":"b",
 +
"number": 63,
 +
"isroot": false,
 +
"next": ["b64"]
 +
},
 +
{
 +
"date":"27-8-2015",
 +
"title":"Colony PCR",
 +
"category":"b",
 +
"number": 64,
 +
"isroot": false,
 +
"next": ["b65"]
 +
},
 +
{
 +
"date":"28-8-2015",
 +
"title":"Ligation",
 +
"category":"b",
 +
"number": 65,
 +
"isroot": false,
 +
"next": ["b66"]
 +
},
 +
{
 +
"date":"28-08-2015",
 +
"title":"Miniprep",
 +
"category":"b",
 +
"number": 66,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"19-08-2015",
 +
"title":"Digestion",
 +
"category":"b",
 +
"number": 67,
 +
"isroot": false,
 +
"next": ["b68"]
 +
},
 +
{
 +
"date":"20-08-2015",
 +
"title":"Ligation",
 +
"category":"b",
 +
"number": 68,
 +
"isroot": false,
 +
"next": ["b69"]
 +
},
 +
{
 +
"date":"21-08-2015",
 +
"title":"Colony PCR",
 +
"category":"b",
 +
"number": 69,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"Digestion",
 +
"category":"b",
 +
"number": 70,
 +
"isroot": false,
 +
"next": ["b71"]
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"PCR Purification",
 +
"category":"b",
 +
"number": 71,
 +
"isroot": false,
 +
"next": ["b72"]
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"Transformation",
 +
"category":"b",
 +
"number": 72,
 
"isroot": false,
 
"isroot": false,
 
"next": null
 
"next": null
 +
},
 +
{
 +
"date":"31-08-2015",
 +
"title":"PCR",
 +
"category":"fr",
 +
"number": 1,
 +
"isroot": true,
 +
"next": ["fr2"]
 
},
 
},
 
{
 
{
"date":"24-08-2015",
+
"date":"31-08-2015",
"title":"Psalt Ligation tasA",  
+
"title":"Purification",
"category":"zp",
+
"category":"fr",
 +
"number": 2,
 +
"isroot": false,
 +
"next": ["fr3"]
 +
},
 +
{
 +
"date":"31-08-2015",
 +
"title":"Ligation",
 +
"category":"fr",
 +
"number": 3,
 +
"isroot": false,
 +
"next": ["fr4"]
 +
},
 +
{
 +
"date":"31-08-2015",
 +
"title":"Gel Purification",
 +
"category":"fr",
 +
"number": 4,
 +
"isroot": false,
 +
"next": ["fr5"]
 +
},
 +
{
 +
"date":"31-08-2015",
 +
"title":"Ligation",
 +
"category":"fr",
 +
"number": 5,
 +
"isroot": false,
 +
"next": ["fr6"]
 +
},
 +
{
 +
"date":"31-08-2015",
 +
"title":"Transformation",
 +
"category":"fr",
 +
"number": 6,
 +
"isroot": false,
 +
"next": ["fr7"]
 +
},
 +
{
 +
"date":"01-09-2015",
 +
"title":"Colony PCR",
 +
"category":"fr",
 +
"number": 7,
 +
"isroot": false,
 +
"next": ["fr8"]
 +
},
 +
{
 +
"date":"02-09-2015",
 +
"title":"Transformation",
 +
"category":"fr",
 +
"number": 8,
 +
"isroot": false,
 +
"next": ["fr9"]
 +
},
 +
{
 +
"date":"02-09-2015",
 +
"title":"Miniprep",
 +
"category":"fr",
 +
"number": 9,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"02-09-2015",
 +
"title":"Glycerol Culture",
 +
"category":"fr",
 +
"number": 10,
 +
"isroot": false,
 +
"next": ["fr11"]
 +
},
 +
{
 +
"date":"11-09-2015",
 +
"title":"IPTG",
 +
"category":"fr",
 +
"number": 11,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"128 Plates",
 +
"category":"py",
 +
"number": 1,
 +
"isroot": true,
 +
"next": ["py2"]
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"LB+G Wet",
 +
"category":"py",
 +
"number": 2,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"07-08-2015",
 +
"title":"CFR test",
 +
"category":"py",
 +
"number": 3,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"09-08-2015",
 +
"title":"DFR test",
 +
"category":"py",
 +
"number": 4,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"15-08-2015",
 +
"title":"TSB 2.0 vs TSB*",
 +
"category":"py",
 +
"number": 5,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"15-08-2015",
 +
"title":"Transform with rpf",
 +
"category":"py",
 +
"number": 6,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"11-08-2015",
 +
"title":"Partially Submerged Growth",
 +
"category":"py",
 +
"number": 7,
 +
"isroot": true,
 +
"next": null
 +
},
 +
{
 +
"date":"23-08-2015",
 +
"title":"Plates",
 +
"category":"n",
 +
"number": 1,
 +
"isroot": true,
 +
"next": ["n2","n4"]
 +
},
 +
{
 +
"date":"09-09-2015",
 +
"title":"Kickstarters",
 +
"category":"n",
 +
"number": 2,
 +
"isroot": false,
 +
"next": ["n3"]
 +
},
 +
{
 +
"date":"09-09-2015",
 +
"title":"OCV on Coffee Filters",
 +
"category":"n",
 +
"number": 3,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"OCV on w2",
 +
"category":"n",
 +
"number": 4,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"28-07-2015",
 +
"title":"slrR PCR",
 +
"category":"sl",
 +
"number": 1,
 +
"isroot": true,
 +
"next": ["sl2","sl6"]
 +
},
 +
{
 +
"date":"29-07-2015",
 +
"title":"slrR Restriction",
 +
"category":"sl",
 +
"number": 2,
 +
"isroot": false,
 +
"next": ["sl3"]
 +
},
 +
{
 +
"date":"29-07-2015",
 +
"title":"slrR PCR Purification",
 +
"category":"sl",
 +
"number": 3,
 +
"isroot": false,
 +
"next": ["sl4","sl28"]
 +
},
 +
{
 +
"date":"29-07-2015",
 +
"title":"slrR Ligation in pSB1C3",
 +
"category":"sl",
 +
"number": 28,
 +
"isroot": false,
 +
"next": ["sl29"]
 +
},
 +
{
 +
"date":"30-07-2015",
 +
"title":"SlrR Transformation",
 +
"category":"sl",
 +
"number": 29,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"29-07-2015",
 +
"title":"SlrR Ligation in K823023",
 +
"category":"sl",
 +
"number": 4,
 +
"isroot": false,
 +
"next": ["sl5"]
 +
},
 +
{
 +
"date":"30-07-2015",
 +
"title":"SlrR Transformation",
 +
"category":"sl",
 +
"number": 5,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"SlrR Restriction",
 +
"category":"sl",
 +
"number": 6,
 +
"isroot": false,
 +
"next": ["sl7"]
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"SlrR PCR Purification",
 +
"category":"sl",
 +
"number": 7,
 +
"isroot": false,
 +
"next": ["sl8"]
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"SlrR Ligation in K823023",
 +
"category":"sl",
 +
"number": 8,
 +
"isroot": false,
 +
"next": ["sl9"]
 +
},
 +
{
 +
"date":"17-08-2015",
 +
"title":"SlrR Transformation",
 +
"category":"sl",
 +
"number": 9,
 +
"isroot": false,
 +
"next": ["sl10"]
 +
},
 +
{
 +
"date":"18-08-2015",
 +
"title":"SlrR Colony PCR",
 +
"category":"sl",
 +
"number": 10,
 +
"isroot": false,
 +
"next": ["sl11"]
 +
},
 +
{
 +
"date":"19-08-2015",
 +
"title":"SlrR Miniprep",
 +
"category":"sl",
 +
"number": 11,
 +
"isroot": false,
 +
"next": ["sl12","sl16","sl20","sl13","sl17","sl90","sl14","sl21","sl18","sl24"]
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"SlrR Transformation 3610 JH642",
 +
"category":"sl",
 +
"number": 12,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"SlrR Transformation PY79",
 +
"category":"sl",
 +
"number": 16,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"SlrR Transformation JH642",
 +
"category":"sl",
 +
"number": 20,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"SlrR Transformation 3610 comI",
 +
"category":"sl",
 +
"number": 13,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"SlrR Transformation PY79",
 +
"category":"sl",
 +
"number": 17,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"SlrR Transformation JH642",
 +
"category":"sl",
 +
"number": 90,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"27-08-2015",
 +
"title":"SlrR Transformation PY79",
 +
"category":"sl",
 +
"number": 18,
 +
"isroot": false,
 +
"next": ["sl19"]
 +
},
 +
{
 +
"date":"27-08-2015",
 +
"title":"SlrR Transformation JH642",
 +
"category":"sl",
 +
"number": 21,
 +
"isroot": false,
 +
"next": ["sl22","sl23"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"SlrR Transformation 3610 comI",
 +
"category":"sl",
 +
"number": 14,
 +
"isroot": false,
 +
"next": ["sl15"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"SlrR Streak plate 3610 comI",
 +
"category":"sl",
 +
"number": 15,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"SlrR Streak plate PY79",
 +
"category":"sl",
 +
"number": 19,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"SlrR Streak plate JH642",
 +
"category":"sl",
 +
"number": 22,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"30-08-2015",
 +
"title":"SlrR Streak plate JH642",
 +
"category":"sl",
 +
"number": 23,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"SlrR Transformation Natto",
 +
"category":"sl",
 +
"number": 24,
 +
"isroot": false,
 +
"next": ["sl25","sl26"]
 +
},
 +
{
 +
"date":"01-09-2015",
 +
"title":"SlrR Streak plate Natto",
 +
"category":"sl",
 +
"number": 25,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"02-09-2015",
 +
"title":"SlrR Streak plate Natto",
 +
"category":"sl",
 +
"number": 26,
 +
"isroot": false,
 +
"next": ["sl27"]
 +
},
 +
{
 +
"date":"09-09-2015",
 +
"title":"SlrR Natto biofilms",
 +
"category":"sl",
 +
"number": 27,
 +
"isroot": false,
 +
"next": []
 +
        },
 +
{
 +
"date":"16-04-2015",
 +
"title":"tasA Primer design",
 +
"category":"t",
 +
"number": 1,
 +
"isroot": true,
 +
"next": ["t2","t4"]
 +
},
 +
{
 +
"date":"13-05-2015",
 +
"title":"tasA PCR",
 +
"category":"t",
 +
"number": 2,
 +
"isroot": false,
 +
"next": ["t3"]
 +
},
 +
{
 +
"date":"13-05-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 3,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"15-05-2015",
 +
"title":"tasA PCR",
 +
"category":"t",
 +
"number": 4,
 +
"isroot": false,
 +
"next": ["t5"]
 +
},
 +
{
 +
"date":"18-05-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 5,
 +
"isroot": false,
 +
"next": ["t6","t7"]
 +
},
 +
{
 +
"date":"18-05-2015",
 +
"title":"tasA Assembly PCR",
 +
"category":"t",
 +
"number": 6,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"19-05-2015",
 +
"title":"tasA Gibson Assembly",
 +
"category":"t",
 +
"number": 7,
 +
"isroot": false,
 +
"next": ["t8"]
 +
},
 +
{
 +
"date":"19-05-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 8,
 +
"isroot": false,
 +
"next": ["t9"]
 +
},
 +
{
 +
"date":"19-05-2015",
 +
"title":"tasA PCR",
 +
"category":"t",
 +
"number": 9,
 +
"isroot": false,
 +
"next": ["t10"]
 +
},
 +
{
 +
"date":"20-05-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 10,
 +
"isroot": false,
 +
"next": ["t11"]
 +
},
 +
{
 +
"date":"20-05-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 11,
 +
"isroot": false,
 +
"next": ["t13"]
 +
},
 +
{
 +
"date":"30-04-2015",
 +
"title":"Backbone Digestion",
 +
"category":"t",
 +
"number": 24,
 +
"isroot": true,
 +
"next": ["t25"]
 +
},
 +
{
 +
"date":"30-04-2015",
 +
"title":"Backbone miniprep",
 +
"category":"t",
 +
"number": 25,
 +
"isroot": false,
 +
"next": ["t13","t26"]
 +
},
 +
{
 +
"date":"27-05-2015",
 +
"title":"Backbone Digestion",
 +
"category":"t",
 +
"number": 26,
 +
"isroot": false,
 +
"next": ["t27"]
 +
},
 +
{
 +
"date":"27-05-2015",
 +
"title":"Backbone Digestion",
 +
"category":"t",
 +
"number": 27,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"20-05-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 13,
 +
"isroot": false,
 +
"next": ["t14","t21","t28"]
 +
},
 +
{
 +
"date":"21-05-2015",
 +
"title":"tasA Gel purification",
 +
"category":"t",
 +
"number": 14,
 +
"isroot": false,
 +
"next": ["t15"]
 +
},
 +
{
 +
"date":"21-05-2015",
 +
"title":"tasA Ligation",
 +
"category":"t",
 +
"number": 15,
 +
"isroot": false,
 +
"next": ["t16"]
 +
},
 +
{
 +
"date":"21-05-2015",
 +
"title":"tasA Heat shock transformation",
 +
"category":"t",
 +
"number": 16,
 +
"isroot": false,
 +
"next": ["t18"]
 +
},
 +
{
 +
"date":"27-05-2015",
 +
"title":"tasA Miniprep",
 +
"category":"t",
 +
"number": 18,
 +
"isroot": false,
 +
"next": ["t19"]
 +
},
 +
{
 +
"date":"28-05-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 19,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"02-06-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 20,
 +
"isroot": false,
 +
"next": ["t21"]
 +
},
 +
{
 +
"date":"02-06-2015",
 +
"title":"tasA Gel purification",
 +
"category":"t",
 +
"number": 21,
 +
"isroot": false,
 +
"next": ["t22"]
 +
},
 +
{
 +
"date":"03-06-2015",
 +
"title":"tasA Ligation",
 +
"category":"t",
 +
"number": 22,
 +
"isroot": false,
 +
"next": ["t23"]
 +
},
 +
{
 +
"date":"03-06-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 +
"number": 23,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"08-06-2015",
 +
"title":"tasA PCR",
 +
"category":"t",
 +
"number": 28,
 +
"isroot": false,
 +
"next": ["t29"]
 +
},
 +
{
 +
"date":"08-06-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 29,
 +
"isroot": false,
 +
"next": ["t30"]
 +
},
 +
{
 +
"date":"09-06-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 30,
 +
"isroot": false,
 +
"next": ["t31"]
 +
},
 +
{
 +
"date":"09-06-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 31,
 +
"isroot": false,
 +
"next": ["t32"]
 +
},
 +
{
 +
"date":"09-06-2015",
 +
"title":"tasA Ligation",
 +
"category":"t",
 +
"number": 32,
 +
"isroot": false,
 +
"next": ["t33"]
 +
},
 +
{
 +
"date":"09-06-2015",
 +
"title":"tasA Heat shock transformation",
 +
"category":"t",
 +
"number": 33,
 +
"isroot": false,
 +
"next": ["t34"]
 +
},
 +
{
 +
"date":"11-06-2015",
 +
"title":"tasA Miniprep",
 +
"category":"t",
 +
"number": 34,
 +
"isroot": false,
 +
"next": ["t35","t36","t37"]
 +
},
 +
{
 +
"date":"11-06-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 35,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"15-06-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 36,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"16-06-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 37,
 +
"isroot": false,
 +
"next": ["t38"]
 +
},
 +
{
 +
"date":"18-06-2015",
 +
"title":"tasA Sequencing",
 +
"category":"t",
 +
"number": 38,
 +
"isroot": false,
 +
"next": ["t39"]
 +
},
 +
{
 +
"date":"08-07-2015",
 +
"title":"tasA Digestion pSB1C3",
 +
"category":"t",
 +
"number": 39,
 +
"isroot": false,
 +
"next": ["t40"]
 +
},
 +
{
 +
"date":"08-07-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 40,
 +
"isroot": false,
 +
"next": ["t41"]
 +
},
 +
{
 +
"date":"08-07-2015",
 +
"title":"tasA Ligation pSB1C3",
 +
"category":"t",
 +
"number": 41,
 +
"isroot": false,
 +
"next": ["t42"]
 +
},
 +
{
 +
"date":"08-07-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 +
"number": 42,
 +
"isroot": false,
 +
"next": ["t43"]
 +
},
 +
{
 +
"date":"10-07-2015",
 +
"title":"tasA Miniprep",
 +
"category":"t",
 +
"number": 43,
 +
"isroot": false,
 +
"next": ["t44","t69","t61","t75"]
 +
},
 +
{
 +
"date":"16-07-2015",
 +
"title":"tasA Digestion K23",
 +
"category":"t",
 +
"number": 44,
 +
"isroot": false,
 +
"next": ["t45","t52","t56"]
 +
},
 +
{
 +
"date":"16-07-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 45,
 +
"isroot": false,
 +
"next": ["t46"]
 +
},
 +
{
 +
"date":"16-07-2015",
 +
"title":"tasA Ligation",
 +
"category":"t",
 +
"number": 46,
 +
"isroot": false,
 +
"next": ["t47"]
 +
},
 +
{
 +
"date":"16-07-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 +
"number": 47,
 +
"isroot": false,
 +
"next": ["t48"]
 +
},
 +
{
 +
"date":"17-07-2015",
 +
"title":"tasA Miniprep",
 +
"category":"t",
 +
"number": 48,
 +
"isroot": false,
 +
"next": ["t49","t50"]
 +
},
 +
{
 +
"date":"21-07-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 +
"number": 49,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"20-07-2015",
 +
"title":"tasA Sequencing",
 +
"category":"t",
 +
"number": 50,
 +
"isroot": false,
 +
"next": ["t69","t61"]
 +
},
 +
{
 +
"date":"23-07-2015",
 +
"title":"tasA Digestion Psalt",
 +
"category":"t",
 +
"number": 51,
 +
"isroot": true,
 +
"next": ["t52"]
 +
},
 +
{
 +
"date":"23-07-2015",
 +
"title":"tasA PCR purification",
 +
"category":"t",
 +
"number": 52,
 +
"isroot": false,
 +
"next": ["t53"]
 +
},
 +
{
 +
"date":"23-07-2015",
 +
"title":"tasA Ligation",
 +
"category":"t",
 +
"number": 53,
 +
"isroot": false,
 +
"next": ["t54","t55"]
 +
},
 +
{
 +
"date":"31-07-2015",
 +
"title":"tasA Colony PCR",  
 +
"category":"t",
 
"number": 54,
 
"number": 54,
 
"isroot": false,
 
"isroot": false,
"next": ["zp55"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"24-08-2015",
+
"date":"23-07-2015",
"title":"Psalt tasA Transformation",  
+
"title":"tasA Transformation",  
"category":"zp",
+
"category":"t",
 
"number": 55,
 
"number": 55,
 
"isroot": false,
 
"isroot": false,
"next": ["zp56"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"25-08-2015",
+
"date":"28-07-2015",
"title":"Psalt tasA Miniprep",  
+
"title":"tasA Digestion",  
"category":"zp",
+
"category":"t",
 
"number": 56,
 
"number": 56,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["t57"]
 
},
 
},
 
{
 
{
"date":"24-08-2015",
+
"date":"28-07-2015",
"title":"Psalt Ligation GFP",  
+
"title":"tasA PCR purification",
"category":"zp",
+
"category":"t",
 +
"number": 57,
 +
"isroot": false,
 +
"next": ["t58","t59"]
 +
},
 +
{
 +
"date":"28-07-2015",
 +
"title":"tasA Ligation",  
 +
"category":"t",
 +
"number": 58,
 +
"isroot": false,
 +
"next": ["t60"]
 +
},
 +
{
 +
"date":"30-07-2015",
 +
"title":"tasA Sequencing",
 +
"category":"t",
 +
"number": 59,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"29-07-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 
"number": 60,
 
"number": 60,
 
"isroot": false,
 
"isroot": false,
"next": ["zp61"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"24-08-2015",
+
"date":"06-08-2015",
"title":"Psalt GFP Transformation",  
+
"title":"tasA Digestion",  
"category":"zp",
+
"category":"t",
 
"number": 61,
 
"number": 61,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["t62"]
 
},
 
},
 
{
 
{
"date":"26-08-2015",
+
"date":"06-08-2015",
"title":"Psalt Digestion",  
+
"title":"tasA Digestion",  
"category":"zp",
+
"category":"t",
 
"number": 62,
 
"number": 62,
 
"isroot": false,
 
"isroot": false,
"next": ["zp71","zp69","zp63"]
+
"next": ["t63"]
 
},
 
},
 
{
 
{
"date":"26-08-2015",
+
"date":"06-08-2015",
"title":"Psalt Ligation bslA",  
+
"title":"tasA PCR purification",  
"category":"zp",
+
"category":"t",
 
"number": 63,
 
"number": 63,
 
"isroot": false,
 
"isroot": false,
"next": ["zp64"]
+
"next": ["t64"]
 
},
 
},
 
{
 
{
"date":"26-08-2015",
+
"date":"06-08-2015",
"title":"Psalt bslA Transformation",  
+
"title":"tasA Ligation",  
"category":"zp",
+
"category":"t",
 
"number": 64,
 
"number": 64,
 
"isroot": false,
 
"isroot": false,
"next": ["zp68"]
+
"next": ["t65"]
 
},
 
},
 
{
 
{
"date":"28-08-2015",
+
"date":"07-08-2015",
"title":"Psalt bslA Miniprep",  
+
"title":"tasA Transformation",  
"category":"zp",
+
"category":"t",
 
"number": 65,
 
"number": 65,
 
"isroot": false,
 
"isroot": false,
"next": ["zp83"]
+
"next": ["t66"]
 
},
 
},
 
{
 
{
"date":"29-08-2015",
+
"date":"09-08-2015",
"title":"Psalt bslA Colony PCR",  
+
"title":"tasA Colony PCR",  
"category":"zp",
+
"category":"t",
"number": 68,
+
"number": 66,
 
"isroot": false,
 
"isroot": false,
"next": ["zp65"]
+
"next": ["t67"]
 
},
 
},
 
{
 
{
"date":"26-08-2015",
+
"date":"11-08-2015",
"title":"Psalt tasA Ligation",  
+
"title":"tasA Miniprep",  
"category":"zp",
+
"category":"t",
 +
"number": 67,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"13-08-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 
"number": 69,
 
"number": 69,
 
"isroot": false,
 
"isroot": false,
"next": ["zp70"]
+
"next": ["t70"]
 
},
 
},
 
{
 
{
"date":"26-08-2015",
+
"date":"13-08-2015",
"title":"Psalt tasA Transformation",  
+
"title":"tasA PCR purification",  
"category":"zp",
+
"category":"t",
 
"number": 70,
 
"number": 70,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["t71"]
 
},
 
},
 
{
 
{
"date":"26-08-2015",
+
"date":"13-08-2015",
"title":"Psalt GFP Ligation",  
+
"title":"tasA Ligation",  
"category":"zp",
+
"category":"t",
 
"number": 71,
 
"number": 71,
 
"isroot": false,
 
"isroot": false,
"next": ["zp72"]
+
"next": ["t72"]
 
},
 
},
 
{
 
{
"date":"28-08-2015",
+
"date":"14-08-2015",
"title":"Psalt GFP Transformation",  
+
"title":"tasA Transformation",  
"category":"zp",
+
"category":"t",
 
"number": 72,
 
"number": 72,
 
"isroot": false,
 
"isroot": false,
"next": ["zp73"]
+
"next": ["t73","t74"]
 
},
 
},
 
{
 
{
"date":"28-08-2015",
+
"date":"17-08-2015",
"title":"Psalt GFP Miniprep",  
+
"title":"tasA Colony PCR",  
"category":"zp",
+
"category":"t",
 
"number": 73,
 
"number": 73,
 
"isroot": false,
 
"isroot": false,
"next": ["zp74"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"28-08-2015",
+
"date":"18-08-2015",
"title":"Psalt GFP Digestion",  
+
"title":"tasA Colony PCR",  
"category":"zp",
+
"category":"t",
 
"number": 74,
 
"number": 74,
 
"isroot": false,
 
"isroot": false,
"next": ["zp75"]
+
"next": null
 
},
 
},
 
{
 
{
"date":"28-08-2015",
+
"date":"17-08-2015",
"title":"Psalt GFP Purification",  
+
"title":"tasA Digestion",  
"category":"zp",
+
"category":"t",
 
"number": 75,
 
"number": 75,
 
"isroot": false,
 
"isroot": false,
"next": ["zp76"]
+
"next": ["t76"]
 
},
 
},
 
{
 
{
"date":"28-08-2015",
+
"date":"17-08-2015",
"title":"Psalt GFP Ligation",  
+
"title":"tasA Purification",  
"category":"zp",
+
"category":"t",
 
"number": 76,
 
"number": 76,
 
"isroot": false,
 
"isroot": false,
"next": ["zp77"]
+
"next": ["t77"]
 
},
 
},
 
{
 
{
"date":"28-08-2015",
+
"date":"17-08-2015",
"title":"Psalt GFP Transformation",  
+
"title":"tasA Ligation Psalt",  
"category":"zp",
+
"category":"t",
 
"number": 77,
 
"number": 77,
 
"isroot": false,
 
"isroot": false,
"next": ["zp78"]
+
"next": ["t78"]
 
},
 
},
 
{
 
{
"date":"29-08-2015",
+
"date":"17-08-2015",
"title":"Psalt GFP Colony PCR",  
+
"title":"tasA Transformation",  
"category":"zp",
+
"category":"t",
 
"number": 78,
 
"number": 78,
 
"isroot": false,
 
"isroot": false,
"next": ["zp79"]
+
"next": ["t102"]
 
},
 
},
 
{
 
{
"date":"30-08-2015",
+
"date":"18-08-2015",
"title":"Psalt GFP Miniprep",  
+
"title":"tasA Colony PCR",
"category":"zp",
+
"category":"t",
 +
"number": 102,
 +
"isroot": false,
 +
"next": ["t100"]
 +
},
 +
{
 +
"date":"19-08-2015",
 +
"title":"tasA Miniprep",  
 +
"category":"t",
 +
"number": 100,
 +
"isroot": false,
 +
"next": ["t93","t84","t79"]
 +
},
 +
{
 +
"date":"19-08-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 
"number": 79,
 
"number": 79,
 
"isroot": false,
 
"isroot": false,
"next": ["zp80"]
+
"next": ["t80"]
 
},
 
},
 
{
 
{
"date":"31-08-2015",
+
"date":"19-08-2015",
"title":"Psalt GFP Transformation",  
+
"title":"tasA Purification",  
"category":"zp",
+
"category":"t",
 
"number": 80,
 
"number": 80,
 
"isroot": false,
 
"isroot": false,
"next": ["zp81","zp82"]
+
"next": ["t81"]
 
},
 
},
 
{
 
{
"date":"01-09-2015",
+
"date":"20-08-2015",
"title":"Psalt GFP Validation",  
+
"title":"tasA Ligation Psalt",  
"category":"zp",
+
"category":"t",
 
"number": 81,
 
"number": 81,
 
"isroot": false,
 
"isroot": false,
"next": null
+
"next": ["t82"]
 
},
 
},
 
{
 
{
"date":"02-09-2015",
+
"date":"20-08-2015",
"title":"Psalt GFP Validation",  
+
"title":"tasA Transformation",  
"category":"zp",
+
"category":"t",
 
"number": 82,
 
"number": 82,
 +
"isroot": false,
 +
"next": ["t83"]
 +
},
 +
{
 +
"date":"21-08-2015",
 +
"title":"tasA Colony PCR",
 +
"category":"t",
 +
"number": 83,
 
"isroot": false,
 
"isroot": false,
 
"next": null
 
"next": null
 +
},
 +
{
 +
"date":"24-08-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 84,
 +
"isroot": false,
 +
"next": ["t85"]
 +
},
 +
{
 +
"date":"24-08-2015",
 +
"title":"tasA Purification",
 +
"category":"t",
 +
"number": 85,
 +
"isroot": false,
 +
"next": ["t86"]
 +
},
 +
{
 +
"date":"24-08-2015",
 +
"title":"tasA Ligation Psalt",
 +
"category":"t",
 +
"number": 86,
 +
"isroot": false,
 +
"next": ["t87"]
 +
},
 +
{
 +
"date":"24-08-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 +
"number": 87,
 +
"isroot": false,
 +
"next": ["t88"]
 
},
 
},
 
{
 
{
 
"date":"26-08-2015",
 
"date":"26-08-2015",
"title":"GFP Digestion",  
+
"title":"tasA Miniprep",  
"category":"zp",
+
"category":"t",
"number": 103,
+
"number": 88,
 
"isroot": false,
 
"isroot": false,
"next": ["zp102"]
+
"next": ["t89"]
 
},
 
},
 
{
 
{
 
"date":"26-08-2015",
 
"date":"26-08-2015",
"title":"GFP Purification",  
+
"title":"tasA Digestion",  
"category":"zp",
+
"category":"t",
"number": 102,
+
"number": 89,
 
"isroot": false,
 
"isroot": false,
"next": ["zp71"]
+
"next": ["t90"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"tasA Purification",
 +
"category":"t",
 +
"number": 90,
 +
"isroot": false,
 +
"next": ["t91"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"tasA Ligation",
 +
"category":"t",
 +
"number": 91,
 +
"isroot": false,
 +
"next": ["t92"]
 +
},
 +
{
 +
"date":"27-08-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 +
"number": 92,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"28-08-2015",
 +
"title":"tasA Digestion",
 +
"category":"t",
 +
"number": 93,
 +
"isroot": false,
 +
"next": ["t94"]
 +
},
 +
{
 +
"date":"28-08-2015",
 +
"title":"tasA Purification",
 +
"category":"t",
 +
"number": 94,
 +
"isroot": false,
 +
"next": ["t95"]
 +
},
 +
{
 +
"date":"28-08-2015",
 +
"title":"tasA Ligation",
 +
"category":"t",
 +
"number": 95,
 +
"isroot": false,
 +
"next": ["t96"]
 +
},
 +
{
 +
"date":"28-08-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 +
"number": 96,
 +
"isroot": false,
 +
"next": ["t97"]
 +
},
 +
{
 +
"date":"29-08-2015",
 +
"title":"tasA Colony PCR",
 +
"category":"t",
 +
"number": 97,
 +
"isroot": false,
 +
"next": ["t98"]
 +
},
 +
{
 +
"date":"30-08-2015",
 +
"title":"tasA Miniprep",
 +
"category":"t",
 +
"number": 98,
 +
"isroot": false,
 +
"next": ["t99"]
 +
},
 +
{
 +
"date":"31-08-2015",
 +
"title":"tasA Transformation",
 +
"category":"t",
 +
"number": 99,
 +
"isroot": false,
 +
"next": []
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"Grow E. coli pdg",
 +
"category":"th",
 +
"number": 1,
 +
"isroot": true,
 +
"next": ["th2"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Miniprep",
 +
"category":"th",
 +
"number": 2,
 +
"isroot": false,
 +
"next": ["th3"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Restriction",
 +
"category":"th",
 +
"number": 3,
 +
"isroot": false,
 +
"next": ["th4"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Ligation",
 +
"category":"th",
 +
"number": 4,
 +
"isroot": false,
 +
"next": ["th5"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Transform k23",
 +
"category":"th",
 +
"number": 5,
 +
"isroot": false,
 +
"next": ["th6"]
 +
},
 +
{
 +
"date":"27-08-2015",
 +
"title":"Liquid LB",
 +
"category":"th",
 +
"number": 6,
 +
"isroot": false,
 +
"next": ["th7"]
 +
},
 +
{
 +
"date":"27-08-2015",
 +
"title":"Miniprep",
 +
"category":"th",
 +
"number": 7,
 +
"isroot": false,
 +
"next": ["th8"]
 +
},
 +
{
 +
"date":"27-08-2015",
 +
"title":"Restriction Analysis",
 +
"category":"th",
 +
"number": 8,
 +
"isroot": false,
 +
"next": null
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"Grow k23",
 +
"category":"th",
 +
"number": 9,
 +
"isroot": true,
 +
"next": ["th10"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Miniprep",
 +
"category":"th",
 +
"number": 10,
 +
"isroot": false,
 +
"next": ["th11"]
 +
},
 +
{
 +
"date":"26-08-2015",
 +
"title":"Restriction",
 +
"category":"th",
 +
"number": 11,
 +
"isroot": false,
 +
"next": ["th4"]
 +
},
 +
{
 +
"date":"25-08-2015",
 +
"title":"Ordered Primers",
 +
"category":"th",
 +
"number": 12,
 +
"isroot": true,
 +
"next": ["th13"]
 +
},
 +
{
 +
"date":"02-09-2015",
 +
"title":"PCR thrC 2",
 +
"category":"th",
 +
"number": 13,
 +
"isroot": false,
 +
"next": ["th14"]
 +
},
 +
{
 +
"date":"03-09-2015",
 +
"title":"Restriction",
 +
"category":"th",
 +
"number": 14,
 +
"isroot": false,
 +
"next": ["th15"]
 +
},
 +
{
 +
"date":"03-09-2015",
 +
"title":"PCR Purification",
 +
"category":"th",
 +
"number": 15,
 +
"isroot": false,
 +
"next": ["th16"]
 +
},
 +
{
 +
"date":"03-09-2015",
 +
"title":"Ligation",
 +
"category":"th",
 +
"number": 16,
 +
"isroot": false,
 +
"next": ["th17"]
 +
},
 +
{
 +
"date":"03-09-2015",
 +
"title":"Transformation",
 +
"category":"th",
 +
"number": 17,
 +
"isroot": false,
 +
"next": ["th18"]
 
},
 
},
 
{
 
{
 
"date":"04-09-2015",
 
"date":"04-09-2015",
"title":"Psalt Validation",  
+
"title":"Colony PCR",  
"category":"zp",
+
"category":"th",
"number": 83,
+
"number": 18,
 +
"isroot": false,
 +
"next": ["th19"]
 +
},
 +
{
 +
"date":"05-09-2015",
 +
"title":"Liquid Medium",
 +
"category":"th",
 +
"number": 19,
 +
"isroot": false,
 +
"next": ["th20"]
 +
},
 +
{
 +
"date":"05-09-2015",
 +
"title":"Miniprep",
 +
"category":"th",
 +
"number": 20,
 +
"isroot": false,
 +
"next": ["th21"]
 +
},
 +
{
 +
"date":"06-09-2015",
 +
"title":"Restriction with Inserts",
 +
"category":"th",
 +
"number": 21,
 +
"isroot": false,
 +
"next": ["th22"]
 +
},
 +
{
 +
"date":"06-09-2015",
 +
"title":"Ligation",
 +
"category":"th",
 +
"number": 22,
 +
"isroot": false,
 +
"next": ["th23"]
 +
},
 +
{
 +
"date":"06-09-2015",
 +
"title":"Transformation",
 +
"category":"th",
 +
"number": 23,
 +
"isroot": false,
 +
"next": ["th24"]
 +
},
 +
{
 +
"date":"07-09-2015",
 +
"title":"Colony PCR",
 +
"category":"th",
 +
"number": 24,
 +
"isroot": false,
 +
"next": ["th25"]
 +
},
 +
{
 +
"date":"08-09-2015",
 +
"title":"Miniprep",
 +
"category":"th",
 +
"number": 25,
 +
"isroot": false,
 +
"next": ["th26"]
 +
},
 +
{
 +
"date":"08-09-2015",
 +
"title":"Transformation",
 +
"category":"th",
 +
"number": 26,
 
"isroot": false,
 
"isroot": false,
 
"next": []
 
"next": []
 
}
 
}
 
]
 
]

Revision as of 00:25, 21 November 2015

[ { "date":"05-09-2015", "title":"tasA Restriction", "category":"tb", "number": 1, "isroot": true, "next": ["tb3"] }, { "date":"05-09-2015", "title":"tasA bslA ligation", "category":"tb", "number": 3, "isroot": false, "next": ["tb4"] }, { "date":"05-09-2015", "title":"bslA Restriction", "category":"tb", "number": 2, "isroot": true, "next": ["tb3"] }, { "date":"05-09-2015", "title":"tasA bslA Transformation", "category":"tb", "number": 4, "isroot": false, "next": null }, { "date":"06-09-2015", "title":"tasA Restriction", "category":"tb", "number": 5, "isroot": true, "next": ["tb7"] }, { "date":"06-09-2015", "title":"tasA bslA ligation", "category":"tb", "number": 7, "isroot": false, "next": ["tb8"] }, { "date":"06-09-2015", "title":"bslA Restriction", "category":"tb", "number": 6, "isroot": true, "next": ["tb8"] }, { "date":"06-09-2015", "title":"tasA bslA Transformation", "category":"tb", "number": 8, "isroot": false, "next": ["tb9"] }, { "date":"07-09-2015", "title":"tasA bslA Colony PCR", "category":"tb", "number": 9, "isroot": false, "next": ["tb10"] }, { "date":"08-09-2015", "title":"tasA bslA Miniprep", "category":"tb", "number": 10, "isroot": false, "next": ["tb11"] }, { "date":"08-09-2015", "title":"tasA bslA Transformation", "category":"tb", "number": 11, "isroot": false, "next": [] }, { "date":"22-07-2015", "title":"Liquid Medium", "category":"co", "number": 1, "isroot": true, "next": ["co2","co3"] }, { "date":"22-07-2015", "title":"CFR", "category":"co", "number": 2, "isroot": false, "next": null }, { "date":"24-07-2015", "title":"Centrifugation", "category":"co", "number": 3, "isroot": false, "next": ["co4"] }, { "date":"24-07-2015", "title":"Dissolve in PBS", "category":"co", "number": 4, "isroot": false, "next": ["co5"] }, { "date":"24-07-2015", "title":"CFR", "category":"co", "number": 5, "isroot": false, "next": null }, { "date":"20-07-2015", "title":"CFR", "category":"Liquid Medium", "number": 7, "isroot": true, "next": ["co8"] }, { "date":"21-07-2015", "title":"Plate", "category":"co", "number": 8, "isroot": false, "next": ["co9"] }, { "date":"22-07-2015", "title":"DFR", "category":"co", "number": 9, "isroot": false, "next": null }, { "date":"23-07-2015", "title":"Liquid Medium", "category":"co", "number": 10, "isroot": true, "next": ["co11"] }, { "date":"24-07-2015", "title":"Plate", "category":"co", "number": 11, "isroot": false, "next": ["co12"] }, { "date":"25-07-2015", "title":"DFR", "category":"co", "number": 12, "isroot": false, "next": null }, { "date":"29-07-2015", "title":"Liquid Medium", "category":"co", "number": 15, "isroot": true, "next": ["co16","co17"] }, { "date":"30-07-2015", "title":"Plate", "category":"co", "number": 16, "isroot": false, "next": ["co17"] }, { "date":"30-07-2015", "title":"Liquid Medium", "category":"co", "number": 17, "isroot": false, "next": ["co21"] }, { "date":"01-08-2015", "title":"Liquid Medium", "category":"co", "number": 18, "isroot": true, "next": ["co19"] }, { "date":"03-08-2015", "title":"Plated", "category":"co", "number": 19, "isroot": false, "next": ["co20"] }, { "date":"05-08-2015", "title":"DFR", "category":"co", "number": 20, "isroot": false, "next": null }, { "date":"05-08-2015", "title":"Plates", "category":"co", "number": 21, "isroot": false, "next": null }, { "date":"15-08-2015", "title":"Transformation with RFP", "category":"co", "number": 22, "isroot": true, "next": null }, { "date":"04-08-2015", "title":"Transformation with GFP", "category":"co", "number": 23, "isroot": true, "next": null }, { "date":"22-08-2015", "title":"Transformation with GFP", "category":"co", "number": 24, "isroot": true, "next": null }, { "date":"15-08-2015", "title":"TSB 2.0 vs TSBstar", "category":"ab", "number": 1, "isroot": true, "next": null }, { "date":"09-09-2015", "title":"Transform comI", "category":"ab", "number": 2, "isroot": true, "next": ["ab3"] }, { "date":"11-09-2015", "title":"Transform with slrR", "category":"ab", "number": 3, "isroot": false, "next": ["ab4"] }, { "date":"29-08-2015", "title":"Streak Plates", "category":"ab", "number": 4, "isroot": false, "next": ["ab5","ab6","ab7"] }, { "date":"01-09-2015", "title":"New Streak Plates", "category":"ab", "number": 5, "isroot": false, "next": [] }, { "date":"30-08-2015", "title":"New Streak Plates", "category":"ab", "number": 6, "isroot": false, "next": [] }, { "date":"03-09-2015", "title":"JH642 Streak Plate", "category":"ab", "number": 7, "isroot": false, "next": [] }, { "date":"29-08-2015", "title":"Transform Natto", "category":"ab", "number": 8, "isroot": true, "next": [] }, { "date":"14-08-2015", "title":"Transform JH642, PY79", "category":"ab", "number": 9, "isroot": false, "next": ["ab4"] }, { "date":"26-08-2015", "title":"Transform JH642, PY79, comI", "category":"ab", "number": 10, "isroot": false, "next": ["ab4"] }, { "date":"25-08-2015", "title":"Transform JH642, PY79", "category":"ab", "number": 11, "isroot": false, "next": ["ab4"] }, { "date":"14-08-2015", "title":"Transform JH642, PY79", "category":"ab", "number": 12, "isroot": false, "next": ["ab4"] }, { "date":"28-08-2015", "title":"PCR", "category":"ab", "number": 13, "isroot": true, "next": ["ab14"] }, { "date":"29-08-2015", "title":"A2 Recombination", "category":"ab", "number": 14, "isroot": false, "next": ["ab15"] }, { "date":"29-08-2015", "title":"Restriction", "category":"ab", "number": 15, "isroot": false, "next": ["ab16"] }, { "date":"29-08-2015", "title":"Ligation", "category":"ab", "number": 16, "isroot": false, "next": [] }, { "date":"28-08-2015", "title":"PCR", "category":"a", "number": 17, "isroot": false, "next": ["a18"] }, { "date":"29-08-2015", "title":"A1 Recombination", "category":"a", "number": 18, "isroot": false, "next": ["a19"] }, { "date":"29-08-2015", "title":"Restriction", "category":"a", "number": 19, "isroot": false, "next": ["a20"] }, { "date":"29-08-2015", "title":"Ligation", "category":"a", "number": 20, "isroot": false, "next": [] }, { "date":"03-09-2015", "title":"PCR", "category":"a", "number": 21, "isroot": false, "next": ["a23"] }, { "date":"05-09-2015", "title":"Digestion", "category":"a", "number": 23, "isroot": false, "next": ["a24"] }, { "date":"05-09-2015", "title":"Ligation", "category":"a", "number": 24, "isroot": false, "next": ["a25"] }, { "date":"06-09-2015", "title":"Transform E. coli", "category":"a", "number": 25, "isroot": false, "next": null }, { "date":"02-09-2015", "title":"PCR", "category":"a", "number": 26, "isroot": false, "next": null }, { "date":"26-07-2015", "title":"Transformation", "category":"a", "number": 27, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"Rigidity OD", "category":"a", "number": 29, "isroot": false, "next": null }, { "date":"08-08-2015", "title":"Transformation", "category":"a", "number": 30, "isroot": false, "next": ["a29","a32","a34","a35","a39","a40","a41"] }, { "date":"17-08-2015", "title":"OCV", "category":"a", "number": 31, "isroot": false, "next": [] }, { "date":"15-08-2015", "title":"Transformation", "category":"a", "number": 32, "isroot": false, "next": ["a31","a33"] }, { "date":"16-08-2015", "title":"OCV", "category":"a", "number": 33, "isroot": false, "next": [] }, { "date":"25-07-2015", "title":"Rigidity cfu", "category":"a", "number": 34, "isroot": false, "next": [] }, { "date":"24-07-2015", "title":"Rigidity OD", "category":"a", "number": 35, "isroot": false, "next": [] }, { "date":"25-07-2015", "title":"bd1807 Purification", "category":"a", "number": 36, "isroot": true, "next": ["a27","a30","a26","a17","a21","a41"] }, { "date":"10-08-2015", "title":"128 Submerged Plates", "category":"a", "number": 37, "isroot": true, "next": [] }, { "date":"20-08-2015", "title":"abrB Submerged Plates", "category":"a", "number": 38, "isroot": true, "next": [] }, { "date":"30-08-2015", "title":"30 vs 37", "category":"a", "number": 39, "isroot": false, "next": null }, { "date":"31-08-2015", "title":"30 vs 37", "category":"a", "number": 40, "isroot": false, "next": [] }, { "date":"08-09-2015", "title":"PCR", "category":"a", "number": 41, "isroot": false, "next": [] }, { "date":"16-04-2015", "title":"Primer Design", "category":"b", "number": 1, "isroot": true, "next": ["b2","b3","b4"] }, { "date":"15-08-2015", "title":"PCR II", "category":"b", "number": 2, "isroot": false, "next": null }, { "date":"13-05-2015", "title":"PCR I", "category":"b", "number": 3, "isroot": false, "next": null }, { "date":"17-05-2015", "title":"PCR III", "category":"b", "number": 4, "isroot": false, "next": null }, { "date":"21-05-2015", "title":"New Primers", "category":"b", "number": 5, "isroot": true, "next": ["b6"] }, { "date":"08-06-2015", "title":"PCR 168", "category":"b", "number": 6, "isroot": false, "next": ["b7"] }, { "date":"08-06-2015", "title":"Purification", "category":"b", "number": 7, "isroot": false, "next": ["b8"] }, { "date":"09-06-2015", "title":"Assembly PCR", "category":"b", "number": 8, "isroot": false, "next": ["b9"] }, { "date":"09-06-2015", "title":"Purification", "category":"b", "number": 9, "isroot": false, "next": ["b10"] }, { "date":"10-6-2015", "title":"Digestion", "category":"b", "number": 10, "isroot": false, "next": ["b11"] }, { "date":"10-06-2015", "title":"PCR Purification", "category":"b", "number": 11, "isroot": false, "next": ["b12","b26","b23"] }, { "date":"16-07-2015", "title":"Ligation with Psalt", "category":"b", "number": 12, "isroot": false, "next": ["b13"] }, { "date":"16-07-2015", "title":"PCR Purification", "category":"b", "number": 13, "isroot": false, "next": ["b14"] }, { "date":"16-07-2015", "title":"Ligation with Psalt", "category":"b", "number": 14, "isroot": false, "next": ["b15"] }, { "date":"16-07-2015", "title":"PCR Purification", "category":"b", "number": 15, "isroot": false, "next": ["b16"] }, { "date":"17-07-2015", "title":"Transformation", "category":"b", "number": 16, "isroot": false, "next": ["b17"] }, { "date":"17-07-2015", "title":"Colony PCR", "category":"b", "number": 17, "isroot": false, "next": ["b18"] }, { "date":"17-07-2015", "title":"Liquid Culture", "category":"b", "number": 18, "isroot": false, "next": ["b19"] }, { "date":"18-08-2015", "title":"Miniprep", "category":"b", "number": 19, "isroot": false, "next": ["b20","b21"] }, { "date":"20-07-2015", "title":"Sequencing", "category":"b", "number": 20, "isroot": false, "next": null }, { "date":"21-07-2015", "title":"Transformation", "category":"b", "number": 21, "isroot": false, "next": [] }, { "date":"23-07-2015", "title":"Digest with Psalt k23", "category":"b", "number": 22, "isroot": false, "next": ["b23"] }, { "date":"23-07-2015", "title":"PCR Purification", "category":"b", "number": 23, "isroot": false, "next": ["b24"] }, { "date":"23-07-2015", "title":"Ligation", "category":"b", "number": 24, "isroot": false, "next": ["b25"] }, { "date":"23-07-2015", "title":"Plate", "category":"b", "number": 25, "isroot": false, "next": null }, { "date":"10-6-2015", "title":"Ligation k23", "category":"b", "number": 26, "isroot": false, "next": ["b27"] }, { "date":"10-06-2015", "title":"Transformation E. coli", "category":"b", "number": 27, "isroot": false, "next": ["b28"] }, { "date":"11-06-2015", "title":"Liquid Medium", "category":"b", "number": 28, "isroot": false, "next": ["b29"] }, { "date":"12-06-2015", "title":"Miniprep", "category":"b", "number": 29, "isroot": false, "next": ["b30","b31"] }, { "date":"15-06-2015", "title":"Digestion", "category":"b", "number": 30, "isroot": false, "next": null }, { "date":"16-06-2015", "title":"Digestion", "category":"b", "number": 31, "isroot": false, "next": null }, { "date":"08-07-2015", "title":"Digestion psBlc3", "category":"b", "number": 32, "isroot": true, "next": ["b33"] }, { "date":"08-07-2015", "title":"PCR Purification", "category":"b", "number": 33, "isroot": false, "next": ["b34"] }, { "date":"08-07-2015", "title":"Ligation", "category":"b", "number": 34, "isroot": false, "next": ["b35"] }, { "date":"09-07-2015", "title":"Transformation", "category":"b", "number": 35, "isroot": false, "next": ["b36"] }, { "date":"09-07-2015", "title":"Liquid Medium", "category":"b", "number": 36, "isroot": false, "next": ["b37"] }, { "date":"10-07-2015", "title":"Miniprep", "category":"b", "number": 37, "isroot": false, "next": ["b67","b38","b46","b52","b54","b66","b55","b56"] }, { "date":"06-08-2015", "title":"Digestion", "category":"b", "number": 38, "isroot": false, "next": ["b39"] }, { "date":"06-08-2015", "title":"PCR Purification", "category":"b", "number": 39, "isroot": false, "next": ["b40"] }, { "date":"06-08-2015", "title":"Ligation", "category":"b", "number": 40, "isroot": false, "next": ["b41"] }, { "date":"07-08-2015", "title":"Transformation", "category":"b", "number": 41, "isroot": false, "next": ["b42"] }, { "date":"09-08-2015", "title":"Colony PCR", "category":"b", "number": 42, "isroot": false, "next": ["b43"] }, { "date":"09-08-2015", "title":"Overnight Culture", "category":"b", "number": 43, "isroot": false, "next": ["b44"] }, { "date":"11-08-2015", "title":"Miniprep", "category":"b", "number": 44, "isroot": false, "next": ["b45"] }, { "date":"13-08-2015", "title":"Check concentrations", "category":"b", "number": 45, "isroot": false, "next": null }, { "date":"28-07-2015", "title":"Psalt Digestion", "category":"b", "number": 46, "isroot": false, "next": ["b47","b49"] }, { "date":"28-07-2015", "title":"Ligation", "category":"b", "number": 47, "isroot": false, "next": ["b48"] }, { "date":"29-07-2015", "title":"Transformation", "category":"b", "number": 48, "isroot": false, "next": [] }, { "date":"29-07-2015", "title":"PCR Purification", "category":"b", "number": 49, "isroot": false, "next": ["b50"] }, { "date":"28-07-2015", "title":"Ligation", "category":"b", "number": 50, "isroot": false, "next": ["b51"] }, { "date":"29-07-2015", "title":"Transformation", "category":"b", "number": 51, "isroot": false, "next": [] }, { "date":"20-08-2015", "title":"Ligation", "category":"b", "number": 52, "isroot": false, "next": ["b53"] }, { "date":"21-08-2015", "title":"Colony PCR", "category":"b", "number": 53, "isroot": false, "next": null }, { "date":"30-07-2015", "title":"Sequencing", "category":"b", "number": 54, "isroot": false, "next": [] }, { "date":"30-08-2015", "title":"Transformation", "category":"b", "number": 55, "isroot": false, "next": [] }, { "date":"24-08-2015", "title":"Digestion Psalt", "category":"b", "number": 56, "isroot": false, "next": ["b57"] }, { "date":"24-8-2015", "title":"PCR Purification", "category":"b", "number": 57, "isroot": false, "next": ["b58"] }, { "date":"24-08-2015", "title":"Ligation", "category":"b", "number": 58, "isroot": false, "next": ["b59"] }, { "date":"24-08-2015", "title":"Transformation", "category":"b", "number": 59, "isroot": false, "next": ["b60"] }, { "date":"26-08-2015", "title":"Miniprep", "category":"b", "number": 60, "isroot": false, "next": ["b61"] }, { "date":"26-08-2015", "title":"Digest k23", "category":"b", "number": 61, "isroot": false, "next": ["b62"] }, { "date":"26-08-2015", "title":"Ligation", "category":"b", "number": 62, "isroot": false, "next": ["b63"] }, { "date":"27-08-2015", "title":"PCR I", "category":"b", "number": 63, "isroot": false, "next": ["b64"] }, { "date":"27-8-2015", "title":"Colony PCR", "category":"b", "number": 64, "isroot": false, "next": ["b65"] }, { "date":"28-8-2015", "title":"Ligation", "category":"b", "number": 65, "isroot": false, "next": ["b66"] }, { "date":"28-08-2015", "title":"Miniprep", "category":"b", "number": 66, "isroot": false, "next": [] }, { "date":"19-08-2015", "title":"Digestion", "category":"b", "number": 67, "isroot": false, "next": ["b68"] }, { "date":"20-08-2015", "title":"Ligation", "category":"b", "number": 68, "isroot": false, "next": ["b69"] }, { "date":"21-08-2015", "title":"Colony PCR", "category":"b", "number": 69, "isroot": false, "next": [] }, { "date":"17-08-2015", "title":"Digestion", "category":"b", "number": 70, "isroot": false, "next": ["b71"] }, { "date":"17-08-2015", "title":"PCR Purification", "category":"b", "number": 71, "isroot": false, "next": ["b72"] }, { "date":"17-08-2015", "title":"Transformation", "category":"b", "number": 72, "isroot": false, "next": null }, { "date":"31-08-2015", "title":"PCR", "category":"fr", "number": 1, "isroot": true, "next": ["fr2"] }, { "date":"31-08-2015", "title":"Purification", "category":"fr", "number": 2, "isroot": false, "next": ["fr3"] }, { "date":"31-08-2015", "title":"Ligation", "category":"fr", "number": 3, "isroot": false, "next": ["fr4"] }, { "date":"31-08-2015", "title":"Gel Purification", "category":"fr", "number": 4, "isroot": false, "next": ["fr5"] }, { "date":"31-08-2015", "title":"Ligation", "category":"fr", "number": 5, "isroot": false, "next": ["fr6"] }, { "date":"31-08-2015", "title":"Transformation", "category":"fr", "number": 6, "isroot": false, "next": ["fr7"] }, { "date":"01-09-2015", "title":"Colony PCR", "category":"fr", "number": 7, "isroot": false, "next": ["fr8"] }, { "date":"02-09-2015", "title":"Transformation", "category":"fr", "number": 8, "isroot": false, "next": ["fr9"] }, { "date":"02-09-2015", "title":"Miniprep", "category":"fr", "number": 9, "isroot": false, "next": [] }, { "date":"02-09-2015", "title":"Glycerol Culture", "category":"fr", "number": 10, "isroot": false, "next": ["fr11"] }, { "date":"11-09-2015", "title":"IPTG", "category":"fr", "number": 11, "isroot": false, "next": [] }, { "date":"17-08-2015", "title":"128 Plates", "category":"py", "number": 1, "isroot": true, "next": ["py2"] }, { "date":"17-08-2015", "title":"LB+G Wet", "category":"py", "number": 2, "isroot": false, "next": null }, { "date":"07-08-2015", "title":"CFR test", "category":"py", "number": 3, "isroot": true, "next": null }, { "date":"09-08-2015", "title":"DFR test", "category":"py", "number": 4, "isroot": true, "next": null }, { "date":"15-08-2015", "title":"TSB 2.0 vs TSB*", "category":"py", "number": 5, "isroot": true, "next": null }, { "date":"15-08-2015", "title":"Transform with rpf", "category":"py", "number": 6, "isroot": true, "next": null }, { "date":"11-08-2015", "title":"Partially Submerged Growth", "category":"py", "number": 7, "isroot": true, "next": null }, { "date":"23-08-2015", "title":"Plates", "category":"n", "number": 1, "isroot": true, "next": ["n2","n4"] }, { "date":"09-09-2015", "title":"Kickstarters", "category":"n", "number": 2, "isroot": false, "next": ["n3"] }, { "date":"09-09-2015", "title":"OCV on Coffee Filters", "category":"n", "number": 3, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"OCV on w2", "category":"n", "number": 4, "isroot": false, "next": null }, { "date":"28-07-2015", "title":"slrR PCR", "category":"sl", "number": 1, "isroot": true, "next": ["sl2","sl6"] }, { "date":"29-07-2015", "title":"slrR Restriction", "category":"sl", "number": 2, "isroot": false, "next": ["sl3"] }, { "date":"29-07-2015", "title":"slrR PCR Purification", "category":"sl", "number": 3, "isroot": false, "next": ["sl4","sl28"] }, { "date":"29-07-2015", "title":"slrR Ligation in pSB1C3", "category":"sl", "number": 28, "isroot": false, "next": ["sl29"] }, { "date":"30-07-2015", "title":"SlrR Transformation", "category":"sl", "number": 29, "isroot": false, "next": [] }, { "date":"29-07-2015", "title":"SlrR Ligation in K823023", "category":"sl", "number": 4, "isroot": false, "next": ["sl5"] }, { "date":"30-07-2015", "title":"SlrR Transformation", "category":"sl", "number": 5, "isroot": false, "next": null }, { "date":"17-08-2015", "title":"SlrR Restriction", "category":"sl", "number": 6, "isroot": false, "next": ["sl7"] }, { "date":"17-08-2015", "title":"SlrR PCR Purification", "category":"sl", "number": 7, "isroot": false, "next": ["sl8"] }, { "date":"17-08-2015", "title":"SlrR Ligation in K823023", "category":"sl", "number": 8, "isroot": false, "next": ["sl9"] }, { "date":"17-08-2015", "title":"SlrR Transformation", "category":"sl", "number": 9, "isroot": false, "next": ["sl10"] }, { "date":"18-08-2015", "title":"SlrR Colony PCR", "category":"sl", "number": 10, "isroot": false, "next": ["sl11"] }, { "date":"19-08-2015", "title":"SlrR Miniprep", "category":"sl", "number": 11, "isroot": false, "next": ["sl12","sl16","sl20","sl13","sl17","sl90","sl14","sl21","sl18","sl24"] }, { "date":"25-08-2015", "title":"SlrR Transformation 3610 JH642", "category":"sl", "number": 12, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"SlrR Transformation PY79", "category":"sl", "number": 16, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"SlrR Transformation JH642", "category":"sl", "number": 20, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"SlrR Transformation 3610 comI", "category":"sl", "number": 13, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"SlrR Transformation PY79", "category":"sl", "number": 17, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"SlrR Transformation JH642", "category":"sl", "number": 90, "isroot": false, "next": null }, { "date":"27-08-2015", "title":"SlrR Transformation PY79", "category":"sl", "number": 18, "isroot": false, "next": ["sl19"] }, { "date":"27-08-2015", "title":"SlrR Transformation JH642", "category":"sl", "number": 21, "isroot": false, "next": ["sl22","sl23"] }, { "date":"26-08-2015", "title":"SlrR Transformation 3610 comI", "category":"sl", "number": 14, "isroot": false, "next": ["sl15"] }, { "date":"29-08-2015", "title":"SlrR Streak plate 3610 comI", "category":"sl", "number": 15, "isroot": false, "next": [] }, { "date":"29-08-2015", "title":"SlrR Streak plate PY79", "category":"sl", "number": 19, "isroot": false, "next": null }, { "date":"29-08-2015", "title":"SlrR Streak plate JH642", "category":"sl", "number": 22, "isroot": false, "next": null }, { "date":"30-08-2015", "title":"SlrR Streak plate JH642", "category":"sl", "number": 23, "isroot": false, "next": null }, { "date":"29-08-2015", "title":"SlrR Transformation Natto", "category":"sl", "number": 24, "isroot": false, "next": ["sl25","sl26"] }, { "date":"01-09-2015", "title":"SlrR Streak plate Natto", "category":"sl", "number": 25, "isroot": false, "next": null }, { "date":"02-09-2015", "title":"SlrR Streak plate Natto", "category":"sl", "number": 26, "isroot": false, "next": ["sl27"] }, { "date":"09-09-2015", "title":"SlrR Natto biofilms", "category":"sl", "number": 27, "isroot": false, "next": []

       },

{ "date":"16-04-2015", "title":"tasA Primer design", "category":"t", "number": 1, "isroot": true, "next": ["t2","t4"] }, { "date":"13-05-2015", "title":"tasA PCR", "category":"t", "number": 2, "isroot": false, "next": ["t3"] }, { "date":"13-05-2015", "title":"tasA PCR purification", "category":"t", "number": 3, "isroot": false, "next": null }, { "date":"15-05-2015", "title":"tasA PCR", "category":"t", "number": 4, "isroot": false, "next": ["t5"] }, { "date":"18-05-2015", "title":"tasA PCR purification", "category":"t", "number": 5, "isroot": false, "next": ["t6","t7"] }, { "date":"18-05-2015", "title":"tasA Assembly PCR", "category":"t", "number": 6, "isroot": false, "next": null }, { "date":"19-05-2015", "title":"tasA Gibson Assembly", "category":"t", "number": 7, "isroot": false, "next": ["t8"] }, { "date":"19-05-2015", "title":"tasA PCR purification", "category":"t", "number": 8, "isroot": false, "next": ["t9"] }, { "date":"19-05-2015", "title":"tasA PCR", "category":"t", "number": 9, "isroot": false, "next": ["t10"] }, { "date":"20-05-2015", "title":"tasA PCR purification", "category":"t", "number": 10, "isroot": false, "next": ["t11"] }, { "date":"20-05-2015", "title":"tasA Digestion", "category":"t", "number": 11, "isroot": false, "next": ["t13"] }, { "date":"30-04-2015", "title":"Backbone Digestion", "category":"t", "number": 24, "isroot": true, "next": ["t25"] }, { "date":"30-04-2015", "title":"Backbone miniprep", "category":"t", "number": 25, "isroot": false, "next": ["t13","t26"] }, { "date":"27-05-2015", "title":"Backbone Digestion", "category":"t", "number": 26, "isroot": false, "next": ["t27"] }, { "date":"27-05-2015", "title":"Backbone Digestion", "category":"t", "number": 27, "isroot": false, "next": null }, { "date":"20-05-2015", "title":"tasA PCR purification", "category":"t", "number": 13, "isroot": false, "next": ["t14","t21","t28"] }, { "date":"21-05-2015", "title":"tasA Gel purification", "category":"t", "number": 14, "isroot": false, "next": ["t15"] }, { "date":"21-05-2015", "title":"tasA Ligation", "category":"t", "number": 15, "isroot": false, "next": ["t16"] }, { "date":"21-05-2015", "title":"tasA Heat shock transformation", "category":"t", "number": 16, "isroot": false, "next": ["t18"] }, { "date":"27-05-2015", "title":"tasA Miniprep", "category":"t", "number": 18, "isroot": false, "next": ["t19"] }, { "date":"28-05-2015", "title":"tasA Digestion", "category":"t", "number": 19, "isroot": false, "next": null }, { "date":"02-06-2015", "title":"tasA Digestion", "category":"t", "number": 20, "isroot": false, "next": ["t21"] }, { "date":"02-06-2015", "title":"tasA Gel purification", "category":"t", "number": 21, "isroot": false, "next": ["t22"] }, { "date":"03-06-2015", "title":"tasA Ligation", "category":"t", "number": 22, "isroot": false, "next": ["t23"] }, { "date":"03-06-2015", "title":"tasA Transformation", "category":"t", "number": 23, "isroot": false, "next": null }, { "date":"08-06-2015", "title":"tasA PCR", "category":"t", "number": 28, "isroot": false, "next": ["t29"] }, { "date":"08-06-2015", "title":"tasA PCR purification", "category":"t", "number": 29, "isroot": false, "next": ["t30"] }, { "date":"09-06-2015", "title":"tasA Digestion", "category":"t", "number": 30, "isroot": false, "next": ["t31"] }, { "date":"09-06-2015", "title":"tasA PCR purification", "category":"t", "number": 31, "isroot": false, "next": ["t32"] }, { "date":"09-06-2015", "title":"tasA Ligation", "category":"t", "number": 32, "isroot": false, "next": ["t33"] }, { "date":"09-06-2015", "title":"tasA Heat shock transformation", "category":"t", "number": 33, "isroot": false, "next": ["t34"] }, { "date":"11-06-2015", "title":"tasA Miniprep", "category":"t", "number": 34, "isroot": false, "next": ["t35","t36","t37"] }, { "date":"11-06-2015", "title":"tasA Digestion", "category":"t", "number": 35, "isroot": false, "next": null }, { "date":"15-06-2015", "title":"tasA Digestion", "category":"t", "number": 36, "isroot": false, "next": null }, { "date":"16-06-2015", "title":"tasA Digestion", "category":"t", "number": 37, "isroot": false, "next": ["t38"] }, { "date":"18-06-2015", "title":"tasA Sequencing", "category":"t", "number": 38, "isroot": false, "next": ["t39"] }, { "date":"08-07-2015", "title":"tasA Digestion pSB1C3", "category":"t", "number": 39, "isroot": false, "next": ["t40"] }, { "date":"08-07-2015", "title":"tasA PCR purification", "category":"t", "number": 40, "isroot": false, "next": ["t41"] }, { "date":"08-07-2015", "title":"tasA Ligation pSB1C3", "category":"t", "number": 41, "isroot": false, "next": ["t42"] }, { "date":"08-07-2015", "title":"tasA Transformation", "category":"t", "number": 42, "isroot": false, "next": ["t43"] }, { "date":"10-07-2015", "title":"tasA Miniprep", "category":"t", "number": 43, "isroot": false, "next": ["t44","t69","t61","t75"] }, { "date":"16-07-2015", "title":"tasA Digestion K23", "category":"t", "number": 44, "isroot": false, "next": ["t45","t52","t56"] }, { "date":"16-07-2015", "title":"tasA PCR purification", "category":"t", "number": 45, "isroot": false, "next": ["t46"] }, { "date":"16-07-2015", "title":"tasA Ligation", "category":"t", "number": 46, "isroot": false, "next": ["t47"] }, { "date":"16-07-2015", "title":"tasA Transformation", "category":"t", "number": 47, "isroot": false, "next": ["t48"] }, { "date":"17-07-2015", "title":"tasA Miniprep", "category":"t", "number": 48, "isroot": false, "next": ["t49","t50"] }, { "date":"21-07-2015", "title":"tasA Transformation", "category":"t", "number": 49, "isroot": false, "next": null }, { "date":"20-07-2015", "title":"tasA Sequencing", "category":"t", "number": 50, "isroot": false, "next": ["t69","t61"] }, { "date":"23-07-2015", "title":"tasA Digestion Psalt", "category":"t", "number": 51, "isroot": true, "next": ["t52"] }, { "date":"23-07-2015", "title":"tasA PCR purification", "category":"t", "number": 52, "isroot": false, "next": ["t53"] }, { "date":"23-07-2015", "title":"tasA Ligation", "category":"t", "number": 53, "isroot": false, "next": ["t54","t55"] }, { "date":"31-07-2015", "title":"tasA Colony PCR", "category":"t", "number": 54, "isroot": false, "next": null }, { "date":"23-07-2015", "title":"tasA Transformation", "category":"t", "number": 55, "isroot": false, "next": null }, { "date":"28-07-2015", "title":"tasA Digestion", "category":"t", "number": 56, "isroot": false, "next": ["t57"] }, { "date":"28-07-2015", "title":"tasA PCR purification", "category":"t", "number": 57, "isroot": false, "next": ["t58","t59"] }, { "date":"28-07-2015", "title":"tasA Ligation", "category":"t", "number": 58, "isroot": false, "next": ["t60"] }, { "date":"30-07-2015", "title":"tasA Sequencing", "category":"t", "number": 59, "isroot": false, "next": [] }, { "date":"29-07-2015", "title":"tasA Transformation", "category":"t", "number": 60, "isroot": false, "next": null }, { "date":"06-08-2015", "title":"tasA Digestion", "category":"t", "number": 61, "isroot": false, "next": ["t62"] }, { "date":"06-08-2015", "title":"tasA Digestion", "category":"t", "number": 62, "isroot": false, "next": ["t63"] }, { "date":"06-08-2015", "title":"tasA PCR purification", "category":"t", "number": 63, "isroot": false, "next": ["t64"] }, { "date":"06-08-2015", "title":"tasA Ligation", "category":"t", "number": 64, "isroot": false, "next": ["t65"] }, { "date":"07-08-2015", "title":"tasA Transformation", "category":"t", "number": 65, "isroot": false, "next": ["t66"] }, { "date":"09-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 66, "isroot": false, "next": ["t67"] }, { "date":"11-08-2015", "title":"tasA Miniprep", "category":"t", "number": 67, "isroot": false, "next": null }, { "date":"13-08-2015", "title":"tasA Digestion", "category":"t", "number": 69, "isroot": false, "next": ["t70"] }, { "date":"13-08-2015", "title":"tasA PCR purification", "category":"t", "number": 70, "isroot": false, "next": ["t71"] }, { "date":"13-08-2015", "title":"tasA Ligation", "category":"t", "number": 71, "isroot": false, "next": ["t72"] }, { "date":"14-08-2015", "title":"tasA Transformation", "category":"t", "number": 72, "isroot": false, "next": ["t73","t74"] }, { "date":"17-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 73, "isroot": false, "next": null }, { "date":"18-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 74, "isroot": false, "next": null }, { "date":"17-08-2015", "title":"tasA Digestion", "category":"t", "number": 75, "isroot": false, "next": ["t76"] }, { "date":"17-08-2015", "title":"tasA Purification", "category":"t", "number": 76, "isroot": false, "next": ["t77"] }, { "date":"17-08-2015", "title":"tasA Ligation Psalt", "category":"t", "number": 77, "isroot": false, "next": ["t78"] }, { "date":"17-08-2015", "title":"tasA Transformation", "category":"t", "number": 78, "isroot": false, "next": ["t102"] }, { "date":"18-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 102, "isroot": false, "next": ["t100"] }, { "date":"19-08-2015", "title":"tasA Miniprep", "category":"t", "number": 100, "isroot": false, "next": ["t93","t84","t79"] }, { "date":"19-08-2015", "title":"tasA Digestion", "category":"t", "number": 79, "isroot": false, "next": ["t80"] }, { "date":"19-08-2015", "title":"tasA Purification", "category":"t", "number": 80, "isroot": false, "next": ["t81"] }, { "date":"20-08-2015", "title":"tasA Ligation Psalt", "category":"t", "number": 81, "isroot": false, "next": ["t82"] }, { "date":"20-08-2015", "title":"tasA Transformation", "category":"t", "number": 82, "isroot": false, "next": ["t83"] }, { "date":"21-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 83, "isroot": false, "next": null }, { "date":"24-08-2015", "title":"tasA Digestion", "category":"t", "number": 84, "isroot": false, "next": ["t85"] }, { "date":"24-08-2015", "title":"tasA Purification", "category":"t", "number": 85, "isroot": false, "next": ["t86"] }, { "date":"24-08-2015", "title":"tasA Ligation Psalt", "category":"t", "number": 86, "isroot": false, "next": ["t87"] }, { "date":"24-08-2015", "title":"tasA Transformation", "category":"t", "number": 87, "isroot": false, "next": ["t88"] }, { "date":"26-08-2015", "title":"tasA Miniprep", "category":"t", "number": 88, "isroot": false, "next": ["t89"] }, { "date":"26-08-2015", "title":"tasA Digestion", "category":"t", "number": 89, "isroot": false, "next": ["t90"] }, { "date":"26-08-2015", "title":"tasA Purification", "category":"t", "number": 90, "isroot": false, "next": ["t91"] }, { "date":"26-08-2015", "title":"tasA Ligation", "category":"t", "number": 91, "isroot": false, "next": ["t92"] }, { "date":"27-08-2015", "title":"tasA Transformation", "category":"t", "number": 92, "isroot": false, "next": null }, { "date":"28-08-2015", "title":"tasA Digestion", "category":"t", "number": 93, "isroot": false, "next": ["t94"] }, { "date":"28-08-2015", "title":"tasA Purification", "category":"t", "number": 94, "isroot": false, "next": ["t95"] }, { "date":"28-08-2015", "title":"tasA Ligation", "category":"t", "number": 95, "isroot": false, "next": ["t96"] }, { "date":"28-08-2015", "title":"tasA Transformation", "category":"t", "number": 96, "isroot": false, "next": ["t97"] }, { "date":"29-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 97, "isroot": false, "next": ["t98"] }, { "date":"30-08-2015", "title":"tasA Miniprep", "category":"t", "number": 98, "isroot": false, "next": ["t99"] }, { "date":"31-08-2015", "title":"tasA Transformation", "category":"t", "number": 99, "isroot": false, "next": [] }, { "date":"25-08-2015", "title":"Grow E. coli pdg", "category":"th", "number": 1, "isroot": true, "next": ["th2"] }, { "date":"26-08-2015", "title":"Miniprep", "category":"th", "number": 2, "isroot": false, "next": ["th3"] }, { "date":"26-08-2015", "title":"Restriction", "category":"th", "number": 3, "isroot": false, "next": ["th4"] }, { "date":"26-08-2015", "title":"Ligation", "category":"th", "number": 4, "isroot": false, "next": ["th5"] }, { "date":"26-08-2015", "title":"Transform k23", "category":"th", "number": 5, "isroot": false, "next": ["th6"] }, { "date":"27-08-2015", "title":"Liquid LB", "category":"th", "number": 6, "isroot": false, "next": ["th7"] }, { "date":"27-08-2015", "title":"Miniprep", "category":"th", "number": 7, "isroot": false, "next": ["th8"] }, { "date":"27-08-2015", "title":"Restriction Analysis", "category":"th", "number": 8, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"Grow k23", "category":"th", "number": 9, "isroot": true, "next": ["th10"] }, { "date":"26-08-2015", "title":"Miniprep", "category":"th", "number": 10, "isroot": false, "next": ["th11"] }, { "date":"26-08-2015", "title":"Restriction", "category":"th", "number": 11, "isroot": false, "next": ["th4"] }, { "date":"25-08-2015", "title":"Ordered Primers", "category":"th", "number": 12, "isroot": true, "next": ["th13"] }, { "date":"02-09-2015", "title":"PCR thrC 2", "category":"th", "number": 13, "isroot": false, "next": ["th14"] }, { "date":"03-09-2015", "title":"Restriction", "category":"th", "number": 14, "isroot": false, "next": ["th15"] }, { "date":"03-09-2015", "title":"PCR Purification", "category":"th", "number": 15, "isroot": false, "next": ["th16"] }, { "date":"03-09-2015", "title":"Ligation", "category":"th", "number": 16, "isroot": false, "next": ["th17"] }, { "date":"03-09-2015", "title":"Transformation", "category":"th", "number": 17, "isroot": false, "next": ["th18"] }, { "date":"04-09-2015", "title":"Colony PCR", "category":"th", "number": 18, "isroot": false, "next": ["th19"] }, { "date":"05-09-2015", "title":"Liquid Medium", "category":"th", "number": 19, "isroot": false, "next": ["th20"] }, { "date":"05-09-2015", "title":"Miniprep", "category":"th", "number": 20, "isroot": false, "next": ["th21"] }, { "date":"06-09-2015", "title":"Restriction with Inserts", "category":"th", "number": 21, "isroot": false, "next": ["th22"] }, { "date":"06-09-2015", "title":"Ligation", "category":"th", "number": 22, "isroot": false, "next": ["th23"] }, { "date":"06-09-2015", "title":"Transformation", "category":"th", "number": 23, "isroot": false, "next": ["th24"] }, { "date":"07-09-2015", "title":"Colony PCR", "category":"th", "number": 24, "isroot": false, "next": ["th25"] }, { "date":"08-09-2015", "title":"Miniprep", "category":"th", "number": 25, "isroot": false, "next": ["th26"] }, { "date":"08-09-2015", "title":"Transformation", "category":"th", "number": 26, "isroot": false, "next": [] } ]