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| | {{Template:Vanderbilt_Header}} | | {{Template:Vanderbilt_Header}} |
| | {{Template:Vanderbilt_Navbar}} | | {{Template:Vanderbilt_Navbar}} |
| | + | {{Template:Vanderbilt_Style}} |
| | + | |
| | <html> | | <html> |
| | | | |
| − | <style> | + | <div id="breadcrumb_container" class="container" style="position:fixed; top:88px; z-index:2;"> |
| − | | + | <div class="row"> |
| − | html, body {
| + | <div class=" btn-group btn- breadcrumb"> |
| − | height: 100%;
| + | <a href="#" class="btn btn-default"><i class="glyphicon glyphicon-wrench"></i></a> |
| − | background-color: #3e0812;
| + | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Part_Collection" class="btn btn-default">Part Collection</a> |
| − | }
| + | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Optimized_RFPs" class="btn btn-default chosen">Optimized RFPs</a> |
| − | | + | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Stable_Circuits" class="btn btn-default">Stable Circuits</a> |
| − | body {
| + | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Repair_Enzymes" class="btn btn-default">Repair Enzymes</a> |
| − | position: relative;
| + | </div> |
| − | height: 100%;
| + | </div> |
| − | }
| + | |
| − | | + | |
| − | .footer {
| + | |
| − | position: absolute;
| + | |
| − | bottom: 0;
| + | |
| − | width: 100%;
| + | |
| − | /* Set the fixed height of the footer here */
| + | |
| − | background-color: #3e0812;
| + | |
| − | left: 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | .row-height {
| + | |
| − | display: table;
| + | |
| − | table-layout: fixed;
| + | |
| − | height: 100%;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | @keyframes dnaFadeInOut {
| + | |
| − | 0% {opacity:1;}
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| − | 17% {opacity:1;}
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| − | 25% {opacity:0;}
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| − | 92% {opacity:0;}
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| − | 100% {opacity:1;}
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| − | }
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| − | | + | |
| − | @-o-keyframes dnaFadeInOut {
| + | |
| − | 0% {opacity:1;}
| + | |
| − | 17% {opacity:1;}
| + | |
| − | 25% {opacity:0;}
| + | |
| − | 92% {opacity:0;}
| + | |
| − | 100% {opacity:1;}
| + | |
| − | }
| + | |
| − | @-moz-keyframes dnaFadeInOut {
| + | |
| − | 0% {opacity:1;}
| + | |
| − | 17% {opacity:1;}
| + | |
| − | 25% {opacity:0;}
| + | |
| − | 92% {opacity:0;}
| + | |
| − | 100% {opacity:1;}
| + | |
| − | }
| + | |
| − | @-webkit-keyframes dnaFadeInOut {
| + | |
| − | 0% {opacity:1;}
| + | |
| − | 17% {opacity:1;}
| + | |
| − | 25% {opacity:0;}
| + | |
| − | 92% {opacity:0;}
| + | |
| − | 100% {opacity:1;}
| + | |
| − | }
| + | |
| − | | + | |
| − | .dnaStrand img:nth-of-type(1) {
| + | |
| − | -webkit-animation-delay: 6s;
| + | |
| − | -moz-animation-delay: 6s;
| + | |
| − | -o-animation-delay: 6s;
| + | |
| − | animation-delay: 6s;
| + | |
| − | }
| + | |
| − | .dnaStrand img:nth-of-type(2) {
| + | |
| − | -webkit-animation-delay: 4s;
| + | |
| − | -moz-animation-delay: 4s;
| + | |
| − | -o-animation-delay: 4s;
| + | |
| − | animation-delay: 4s;
| + | |
| − | }
| + | |
| − | .dnaStrand img:nth-of-type(3) {
| + | |
| − | -webkit-animation-delay: 2s;
| + | |
| − | -moz-animation-delay: 2s;
| + | |
| − | -o-animation-delay: 2s;
| + | |
| − | animation-delay: 2s;
| + | |
| − | }
| + | |
| − | .dnaStrand img:nth-of-type(4) {
| + | |
| − | -webkit-animation-delay: 0;
| + | |
| − | -moz-animation-delay: 0;
| + | |
| − | -o-animation-delay: 0;
| + | |
| − | animation-delay: 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | .dnaStrand {
| + | |
| − | position: relative;
| + | |
| − | width: 100%;
| + | |
| − | height: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | .dnaStrand img {
| + | |
| − | position: absolute;
| + | |
| − | height: 95%;
| + | |
| − | width: 95%;
| + | |
| − | vertical-align: top;
| + | |
| − | }
| + | |
| − |
| + | |
| − | .dnaStrand img {
| + | |
| − | -webkit-animation-name: dnaFadeInOut;
| + | |
| − | -webkit-animation-timing-function: ease-in-out;
| + | |
| − | -webkit-animation-iteration-count: infinite;
| + | |
| − | -webkit-animation-duration: 8s;
| + | |
| − | | + | |
| − | -moz-animation-name: dnaFadeInOut;
| + | |
| − | -moz-animation-timing-function: ease-in-out;
| + | |
| − | -moz-animation-iteration-count: infinite;
| + | |
| − | -moz-animation-duration: 8s;
| + | |
| − | | + | |
| − | -o-animation-name: dnaFadeInOut;
| + | |
| − | -o-animation-timing-function: ease-in-out;
| + | |
| − | -o-animation-iteration-count: infinite;
| + | |
| − | -o-animation-duration: 8s;
| + | |
| − | | + | |
| − | animation-name: dnaFadeInOut;
| + | |
| − | animation-timing-function: ease-in-out;
| + | |
| − | animation-iteration-count: infinite;
| + | |
| − | animation-duration: 8s;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .grid {
| + | |
| − | background-color: #5f212c;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* clear fix */
| + | |
| − | .grid:after {
| + | |
| − | content: '';
| + | |
| − | display: block;
| + | |
| − | clear: both;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* ---- .element-item ---- */
| + | |
| − | | + | |
| − | /* 6 columns, percentage width */
| + | |
| − | .grid-item,
| + | |
| − | .grid-sizer {
| + | |
| − | width: 16.666%;
| + | |
| − | margin: 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .grid-item {
| + | |
| − | float: left;
| + | |
| − | background: #0D8;
| + | |
| − | border: 10px solid #44111a;
| + | |
| − | width: 33.333%;
| + | |
| − | margin: 0px;
| + | |
| − | text-align: center;
| + | |
| − | }
| + | |
| − | | + | |
| − | .grid-item--widthFull { width:100%; }
| + | |
| − | | + | |
| − | .grid-item img {
| + | |
| − | display: block;
| + | |
| − | max-width: 100%;
| + | |
| − | margin: 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .grid-textbox-top {
| + | |
| − | border-left: 0px;
| + | |
| − | border-top: 0px;
| + | |
| − | border-right: 0px;
| + | |
| − | border-bottom: 5px solid #44111a;
| + | |
| − | line-height: 100%;
| + | |
| − | vertical-align: middle;
| + | |
| − | }
| + | |
| − | | + | |
| − | .grid-textbox-mid {
| + | |
| − | border-right: 0px;
| + | |
| − | border-left: 0px;
| + | |
| − | border-top: 5px solid #44111a;
| + | |
| − | border-bottom: 5px solid #44111a;
| + | |
| − | line-height: 100%;
| + | |
| − | vertical-align: middle;
| + | |
| − | }
| + | |
| − | | + | |
| − | .grid-textbox-bot {
| + | |
| − | border-right: 0px;
| + | |
| − | border-left: 0px;
| + | |
| − | border-top: 5px solid #44111a;
| + | |
| − | border-bottom: 0px;
| + | |
| − | line-height: 100%;
| + | |
| − | vertical-align: middle;
| + | |
| − | }
| + | |
| − | | + | |
| − | .carousel-inner > .item > img,
| + | |
| − | .carousel-inner > .item > a > img {
| + | |
| − | width: 100%;
| + | |
| − | margin: auto;
| + | |
| − | }
| + | |
| − | | + | |
| − | </style>
| + | |
| − | | + | |
| − | <div class="row" style="height: 100%; min-height: 100%;"> | + | |
| − | <div class="row-height"> | + | |
| − | <div class="col-lg-1 hidden-sm" style="height:100%"> | + | |
| − | <br>
| + | |
| − | <div class="dnaStrand" alt="dna strand">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/a/a6/Vanderbilt_2015_dna_strand_1.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/d/d1/Vanderbilt_2015_dna_strand_2.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/2/2a/Vanderbilt_2015_dna_strand_3.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/f/f4/Vanderbilt_2015_dna_strand_4.png"/>
| + | |
| − | </div>
| + | |
| | </div> | | </div> |
| | | | |
| − | <div class="col-md-10" style="background-color: #44111a; height: 100%;"> | + | <script> |
| − | <p>Put content here</p>
| + | document.getElementById("header_text").innerHTML = "Optimized RFPs"; |
| | + | </script> |
| | | | |
| − | <!-- footer -->
| + | <div class="row"> |
| − | <div class="row" style="margin:0; border: 0; width: 100%;"> | + | <div class="col-md-10 col-md-offset-1" style="background-color: #44111a; height: 100%;"> |
| − | <div class="col- lg- 2" style="height:100%"> | + | <h2>Optimized RFPs</h2> |
| − | <h3><font color="#7e1d2e">Team</font></h3>
| + | <p> While testing our system for lowering mutation rates, we relied heavily on fluorescent reporter genes with their sequences altered according to our optimization algorithm. These parts were made resistant to a variety of mutagen types, and placed in expression cassettes for studying rates of deleterious mutations <i>in vivo</i>. The part numbers and their corresponding genes are listed below: </p><br> |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Team/About"><font color="#9e515e">About</font></a>
| + | <p>K1673010 Partially UV-Resistant RFP |
| − | <br>
| + | <p>K1673011 Constitutively Expressed Partially UV-Resistant RFP |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Team/Members"><font color="#9e515e">Members</font></a>
| + | <p>K1673101 UV-Resistant RFP |
| − | <br>
| + | <p>K1673102 UV Mutation Optimized RFP, Improved Expression |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Team/Attributes"><font color="#9e515e">Attributes</font></a>
| + | <p>K1673103 UV Mutation Maximizied RFP |
| − | <br>
| + | <p>K1673104 Oxidation-Resistant RFP |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Team/Sponsors"><font color="#9e515e">Sponsors</font></a>
| + | <p>K1673110 IPTG Promoter Standard RFP |
| − | <br>
| + | <p>K1673111 IPTG Promoter UV-Resistant RFP |
| − | </div> | + | <p>K1673112 IPTG Promoter UV-Resistant RFP, Improved Expression |
| − | <div class="col-lg-2" style="height:100%"> | + | <p>K1673113 IPTG Promoter UV Mutation Maximized RFP |
| − | <h3><font color="#7e1d2e">Project</font></h3>
| + | <p>K1673114 IPTG Promoter Oxidation-Resistant RFP |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Background"><font color="#9e515e">Background</font></a>
| + | <p>K1673120 T7 Promoter Standard RFP |
| − | <br>
| + | <p>K1673121 T7 Promoter UV-Resistant RFP |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Sequence"><font color="#9e515e">Sequence</font></a>
| + | <p>K1673124 T7 Promoter Oxidation-Resistant RFP |
| − | <br>
| + | </p> |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Circuit"><font color="#9e515e">Circuit</font></a>
| + | |
| − | <br>
| + | |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Organism"><font color="#9e515e">Organism</font></a>
| + | |
| − | <br>
| + | |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Nanopore"><font color="#9e515e">Nanopore</font></a>
| + | |
| − | <br>
| + | |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Achievements"><font color="#9e515e">Achievements</font></a>
| + | |
| − | <br>
| + | |
| − | </div> | + | |
| − | <div class="col-lg-2" style="height:100%">
| + | |
| − | <h3><font color="#7e1d2e">Parts</font></h3>
| + | |
| | | | |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Part_Collection"><font color="#9e515e">Part Collection</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Optimized_RFPs"><font color="#9e515e">Optimized RFPs</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Visualizing_Evolution"><font color="#9e515e">Visualizing Evolution</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Repair Enzymes"><font color="#9e515e">Repair Enzymes</font></a>
| |
| − | <br>
| |
| − | </div>
| |
| − | <div class="col-lg-2" style="height:100%">
| |
| − | <h3><font color="#7e1d2e">Notebook</font></h3>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/May"><font color="#9e515e">May</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/June"><font color="#9e515e">June</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/July"><font color="#9e515e">July</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/August"><font color="#9e515e">August</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/September"><font color="#9e515e">September</font></a>
| |
| − | <br>
| |
| − | </div>
| |
| − | <div class="col-lg-2" style="height:100%">
| |
| − | <h3><font color="#7e1d2e">Practices</font></h3>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Practices/Safety"><font color="#9e515e">Safety</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Practices/Collaborations"><font color="#9e515e">Collaborations</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Practices/Real_World_Applications"><font color="#9e515e">Applications</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Practices/Interlab"><font color="#9e515e">Interlab</font></a>
| |
| − | <br>
| |
| − | </div>
| |
| − | <div class="col-lg-2" style="height:100%">
| |
| − | <h3><font color="#7e1d2e">Software</font></h3>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Software/Development"><font color="#9e515e">Development</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Software/User_Guide"><font color="#9e515e">User Guide</font></a>
| |
| − | <br>
| |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Software/Stats"><font color="#9e515e">Statistics</font></a>
| |
| − | <br>
| |
| − | </div>
| |
| | </div> | | </div> |
| − | <br>
| |
| − | <!-- /footer -->
| |
| − |
| |
| | </div> | | </div> |
| | | | |
| − | <div class="col-lg-1 hidden-sm" style="height:100%;"> | + | </html> |
| − | <br>
| + | {{Template:Vanderbilt_Footer}} |
| − | <div class="dnaStrand" alt="dna strand">
| + | <html> |
| − | <img src="https://static.igem.org/mediawiki/2015/a/a6/Vanderbilt_2015_dna_strand_1.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/d/d1/Vanderbilt_2015_dna_strand_2.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/2/2a/Vanderbilt_2015_dna_strand_3.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/f/f4/Vanderbilt_2015_dna_strand_4.png"/>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| | </html> | | </html> |
