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| | {{Template:Vanderbilt_Header}} | | {{Template:Vanderbilt_Header}} |
| | + | {{Template:Vanderbilt_Navbar}} |
| | + | {{Template:Vanderbilt_Style}} |
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| | <html> | | <html> |
| − | <style>
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| − | .btn {
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| − | -webkit-border-radius: 0 !important;
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| − | -moz-border-radius: 0 !important;
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| − | border-radius: 0 !important;
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| − | background-color: #3e0812;
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| − | color: #9e515e;
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| − | }
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| − | a:link {
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| − | text-decoration: none;
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| − | color: #9e515e;
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| − | }
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| − | a:visited {
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| − | text-decoration: none;
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| − | color: #9e515e;
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| − | .chosen {
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| − | background-color: #220006;
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| − | color: #3e0812;
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| − | }
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| − | </style>
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| | <div id="breadcrumb_container" class="container" style="position:fixed; top:88px; z-index:2;"> | | <div id="breadcrumb_container" class="container" style="position:fixed; top:88px; z-index:2;"> |
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| | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Part_Collection" class="btn btn-default">Part Collection</a> | | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Part_Collection" class="btn btn-default">Part Collection</a> |
| | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Optimized_RFPs" class="btn btn-default">Optimized RFPs</a> | | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Optimized_RFPs" class="btn btn-default">Optimized RFPs</a> |
| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Visualizing_Evolution" class="btn btn-default">Visualizing Evolution</a> | + | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Stable_Circuits" class="btn btn-default">Stable Circuits</a> |
| | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Repair_Enzymes" class="btn btn-default chosen">Repair Enzymes</a> | | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Repair_Enzymes" class="btn btn-default chosen">Repair Enzymes</a> |
| | </div> | | </div> |
| | </div> | | </div> |
| | </div> | | </div> |
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| − | </html>
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| − | {{Template:Vanderbilt_Navbar}}
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| − | <html>
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| | <script> | | <script> |
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| | </script> | | </script> |
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| − | | + | <div class="row"> |
| − | <style>
| + | <div class="col-md-10 col-md-offset-1" style="background-color: #44111a; height: 100%;"> |
| − | | + | <h2>Repair Enzymes</h2> |
| − | html, body {
| + | <p> To build our genetically-engineered strain of bacteria with decreased susceptibility to mutation, we produced several parts for expression DNA repair enzymes. Each of these genes were themselves optimized by our software to have a minimal number of overall mutation hotspots. Included are enzymes for repairing UV-induced lesions, oxidative damage, and the enzymes necessary to construct our "incorruptible cell". Part numbers and their corresponding genes for each are listed below:</p><br> |
| − | height: 100%;
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| − | background-color: #3e0812;
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| − | }
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| − | | + | |
| − | body {
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| − | position: relative;
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| − | height: 100%;
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| − | }
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| − | | + | |
| − | .footer {
| + | |
| − | position: absolute;
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| − | bottom: 0;
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| − | width: 100%;
| + | |
| − | /* Set the fixed height of the footer here */
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| − | background-color: #3e0812;
| + | |
| − | left: 0;
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| − | }
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| − | | + | |
| − | .row-height {
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| − | display: table;
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| − | table-layout: fixed;
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| − | height: 100%;
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| − | width: 100%;
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| − | | + | |
| − | @keyframes dnaFadeInOut {
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| − | 0% {opacity:1;}
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| − | 17% {opacity:1;}
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| − | 92% {opacity:0;}
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| − | }
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| − | @-o-keyframes dnaFadeInOut {
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| − | 0% {opacity:1;}
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| − | 17% {opacity:1;}
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| − | 25% {opacity:0;}
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| − | 92% {opacity:0;}
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| − | 100% {opacity:1;}
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| − | }
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| − | @-moz-keyframes dnaFadeInOut {
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| − | 0% {opacity:1;}
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| − | 17% {opacity:1;}
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| − | 25% {opacity:0;}
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| − | 92% {opacity:0;}
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| − | 100% {opacity:1;}
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| − | }
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| − | @-webkit-keyframes dnaFadeInOut {
| + | |
| − | 0% {opacity:1;}
| + | |
| − | 17% {opacity:1;}
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| − | 25% {opacity:0;}
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| − | 92% {opacity:0;}
| + | |
| − | 100% {opacity:1;}
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| − | }
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| − | | + | |
| − | .dnaStrand img:nth-of-type(1) {
| + | |
| − | -webkit-animation-delay: 6s;
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| − | -moz-animation-delay: 6s;
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| − | -o-animation-delay: 6s;
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| − | animation-delay: 6s;
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| − | }
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| − | .dnaStrand img:nth-of-type(2) {
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| − | -webkit-animation-delay: 4s;
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| − | -moz-animation-delay: 4s;
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| − | -o-animation-delay: 4s;
| + | |
| − | animation-delay: 4s;
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| − | }
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| − | .dnaStrand img:nth-of-type(3) {
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| − | -webkit-animation-delay: 2s;
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| − | -moz-animation-delay: 2s;
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| − | -o-animation-delay: 2s;
| + | |
| − | animation-delay: 2s;
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| − | }
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| − | .dnaStrand img:nth-of-type(4) {
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| − | -webkit-animation-delay: 0;
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| − | -moz-animation-delay: 0;
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| − | -o-animation-delay: 0;
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| − | animation-delay: 0;
| + | |
| − | }
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| − | | + | |
| − | .dnaStrand {
| + | |
| − | position: relative;
| + | |
| − | width: 100%;
| + | |
| − | height: 100%;
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| − | }
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| − | | + | |
| − | .dnaStrand img {
| + | |
| − | position: absolute;
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| − | height: 95%;
| + | |
| − | width: 95%;
| + | |
| − | vertical-align: top;
| + | |
| − | }
| + | |
| − |
| + | |
| − | .dnaStrand img {
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| − | -webkit-animation-name: dnaFadeInOut;
| + | |
| − | -webkit-animation-timing-function: ease-in-out;
| + | |
| − | -webkit-animation-iteration-count: infinite;
| + | |
| − | -webkit-animation-duration: 8s;
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| − | | + | |
| − | -moz-animation-name: dnaFadeInOut;
| + | |
| − | -moz-animation-timing-function: ease-in-out;
| + | |
| − | -moz-animation-iteration-count: infinite;
| + | |
| − | -moz-animation-duration: 8s;
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| − | | + | |
| − | -o-animation-name: dnaFadeInOut;
| + | |
| − | -o-animation-timing-function: ease-in-out;
| + | |
| − | -o-animation-iteration-count: infinite;
| + | |
| − | -o-animation-duration: 8s;
| + | |
| − | | + | |
| − | animation-name: dnaFadeInOut;
| + | |
| − | animation-timing-function: ease-in-out;
| + | |
| − | animation-iteration-count: infinite;
| + | |
| − | animation-duration: 8s;
| + | |
| − | }
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| − | | + | |
| − | | + | |
| − | .grid {
| + | |
| − | background-color: #5f212c;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* clear fix */
| + | |
| − | .grid:after {
| + | |
| − | content: '';
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| − | display: block;
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| − | clear: both;
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| − | }
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| − | | + | |
| − | /* ---- .element-item ---- */
| + | |
| − | | + | |
| − | /* 6 columns, percentage width */
| + | |
| − | .grid-item,
| + | |
| − | .grid-sizer {
| + | |
| − | width: 16.666%;
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| − | margin: 0px;
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| − | }
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| − | | + | |
| − | .grid-item {
| + | |
| − | float: left;
| + | |
| − | background: #0D8;
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| − | border: 10px solid #44111a;
| + | |
| − | width: 33.333%;
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| − | margin: 0px;
| + | |
| − | text-align: center;
| + | |
| − | }
| + | |
| − | | + | |
| − | .grid-item--widthFull { width:100%; }
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| − | | + | |
| − | .grid-item img {
| + | |
| − | display: block;
| + | |
| − | max-width: 100%;
| + | |
| − | margin: 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .grid-textbox-top {
| + | |
| − | border-left: 0px;
| + | |
| − | border-top: 0px;
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| − | border-right: 0px;
| + | |
| − | border-bottom: 5px solid #44111a;
| + | |
| − | line-height: 100%;
| + | |
| − | vertical-align: middle;
| + | |
| − | }
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| − | | + | |
| − | .grid-textbox-mid {
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| − | border-right: 0px;
| + | |
| − | border-left: 0px;
| + | |
| − | border-top: 5px solid #44111a;
| + | |
| − | border-bottom: 5px solid #44111a;
| + | |
| − | line-height: 100%;
| + | |
| − | vertical-align: middle;
| + | |
| − | }
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| − | | + | |
| − | .grid-textbox-bot {
| + | |
| − | border-right: 0px;
| + | |
| − | border-left: 0px;
| + | |
| − | border-top: 5px solid #44111a;
| + | |
| − | border-bottom: 0px;
| + | |
| − | line-height: 100%;
| + | |
| − | vertical-align: middle;
| + | |
| − | }
| + | |
| − | | + | |
| − | .carousel-inner > .item > img,
| + | |
| − | .carousel-inner > .item > a > img {
| + | |
| − | width: 100%;
| + | |
| − | margin: auto;
| + | |
| − | }
| + | |
| − | | + | |
| − | </style>
| + | |
| − | | + | |
| − | <div class="row" style="height: 100%; min-height: 100%;"> | + | |
| − | <div class="row-height">
| + | |
| − | <div class="col-lg-1 hidden-sm" style="height:100%" id="dnaStrand1"> | + | |
| − | <br>
| + | |
| − | <div class="dnaStrand" alt="dna strand">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/a/a6/Vanderbilt_2015_dna_strand_1.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/d/d1/Vanderbilt_2015_dna_strand_2.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/2/2a/Vanderbilt_2015_dna_strand_3.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/f/f4/Vanderbilt_2015_dna_strand_4.png"/>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="col-md-10" style="background-color: #44111a; height: 100%;">
| + | |
| − | <p> To build our genetically-engineered strain of bacteria with decreased susceptibility to mutation, we produced several parts for expression DNA repair enzymes. Each of these genes were themselves optimized by our software to have a minimal number of overall mutation hotspots. Included are enzymes for repairing UV-induced lesions, oxidative damage, and the enzymes necessary to construct our "incorruptible cell". Part numbers and their corresponding genes for each are listed below:</p> | + | |
| | | | |
| | <p>K1673201 T4 Pyrimidine Dimer Glycosylase (Mutation Optimized) | | <p>K1673201 T4 Pyrimidine Dimer Glycosylase (Mutation Optimized) |
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| | <p>K1673224 T7 Promoter Mutation Optimized T7 Nuclease | | <p>K1673224 T7 Promoter Mutation Optimized T7 Nuclease |
| | </p> | | </p> |
| − |
| |
| − | <!-- footer -->
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| − | <div class="row" style="margin:0; border: 0; width: 100%;">
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| − | <div class="col-lg-2" style="height:100%">
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| − | <h3><font color="#7e1d2e">Team</font></h3>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Team/About"><font color="#9e515e">About</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Team/Members"><font color="#9e515e">Members</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Team/Attributes"><font color="#9e515e">Attributes</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Team/Sponsors"><font color="#9e515e">Sponsors</font></a>
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| − | <br>
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| − | </div>
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| − | <div class="col-lg-2" style="height:100%">
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| − | <h3><font color="#7e1d2e">Project</font></h3>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Background"><font color="#9e515e">Background</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Sequence"><font color="#9e515e">Sequence</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Circuit"><font color="#9e515e">Circuit</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Organism"><font color="#9e515e">Organism</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Nanopore"><font color="#9e515e">Nanopore</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Achievements"><font color="#9e515e">Achievements</font></a>
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| − | <br>
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| − | </div>
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| − | <div class="col-lg-2" style="height:100%">
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| − | <h3><font color="#7e1d2e">Parts</font></h3>
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| − |
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Part_Collection"><font color="#9e515e">Part Collection</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Optimized_RFPs"><font color="#9e515e">Optimized RFPs</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Visualizing_Evolution"><font color="#9e515e">Visualizing Evolution</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Parts/Repair Enzymes"><font color="#9e515e">Repair Enzymes</font></a>
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| − | <br>
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| − | </div>
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| − | <div class="col-lg-2" style="height:100%">
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| − | <h3><font color="#7e1d2e">Notebook</font></h3>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/May"><font color="#9e515e">May</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/June"><font color="#9e515e">June</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/July"><font color="#9e515e">July</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/August"><font color="#9e515e">August</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Notebook/September"><font color="#9e515e">September</font></a>
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| − | <br>
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| − | </div>
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| − | <div class="col-lg-2" style="height:100%">
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| − | <h3><font color="#7e1d2e">Practices</font></h3>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Practices/Safety"><font color="#9e515e">Safety</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Practices/Collaborations"><font color="#9e515e">Collaborations</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Practices/Real_World_Applications"><font color="#9e515e">Applications</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Practices/Interlab"><font color="#9e515e">Interlab</font></a>
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| − | <br>
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| − | </div>
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| − | <div class="col-lg-2" style="height:100%">
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| − | <h3><font color="#7e1d2e">Software</font></h3>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Software/Development"><font color="#9e515e">Development</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Software/User_Guide"><font color="#9e515e">User Guide</font></a>
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| − | <br>
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| − | <a href="https://2015.igem.org/Team:Vanderbilt/Software/Stats"><font color="#9e515e">Statistics</font></a>
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| − | <br>
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| − | </div>
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| | </div> | | </div> |
| − | <br>
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| − | <!-- /footer -->
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| − |
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| | </div> | | </div> |
| | | | |
| − | <div class="col-lg-1 hidden-sm" style="height:100%;" id="dnaStrand2"> | + | </html> |
| − | <br>
| + | {{Template:Vanderbilt_Footer}} |
| − | <div class="dnaStrand" alt="dna strand">
| + | <html> |
| − | <img src="https://static.igem.org/mediawiki/2015/a/a6/Vanderbilt_2015_dna_strand_1.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/d/d1/Vanderbilt_2015_dna_strand_2.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/2/2a/Vanderbilt_2015_dna_strand_3.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/f/f4/Vanderbilt_2015_dna_strand_4.png"/>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
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| | </html> | | </html> |
