Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Ligation fr3"
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{{Team:Groningen/TEMPLATES/EXPERIMENT | {{Team:Groningen/TEMPLATES/EXPERIMENT | ||
− | |title= | + | |title=Restriction ferritin (fr3) |
|protocol=Restriction | |protocol=Restriction | ||
|protocolurl=restriction | |protocolurl=restriction | ||
|goal=Development ferritin | |goal=Development ferritin | ||
− | |description= | + | |description=Restriction of ferritin |
− | |conclusion= | + | |conclusion=Restricted product |
}} | }} |
Latest revision as of 21:46, 21 November 2015
Restriction ferritin (fr3)
Restriction of ferritin
Development ferritin
Restricted product
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
16:00, 31 August 2015 - 18:30, 10 August 2015
Digest ferritin in order to ligate them. Samples were digested for 2 hours at 37 °C.
Component
Amount
Ferritin
10 µL
10x buffer (2.1)
2 µL
PstI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion.