Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Ligation fr3"

 
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|protocolurl=restriction
 
|goal=Development ferritin
 
|goal=Development ferritin
|description=restriction of ferritin  
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|description=Restriction of ferritin  
|conclusion= restricted product
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|conclusion=Restricted product
 
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Latest revision as of 21:46, 21 November 2015

Restriction ferritin (fr3)
Restriction of ferritin
Development ferritin
Restricted product

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>

16:00, 31 August 2015 - 18:30, 10 August 2015
Digest ferritin in order to ligate them. Samples were digested for 2 hours at 37 °C.
Component
Amount
Ferritin
10 µL
10x buffer (2.1)
2 µL
PstI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion.
Harm Ruesink