Difference between revisions of "Team:Vanderbilt/Project/Achievements"
| (26 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{Template:Vanderbilt_Header}} | {{Template:Vanderbilt_Header}} | ||
| + | {{Template:Vanderbilt_Navbar}} | ||
{{Template:Vanderbilt_Style}} | {{Template:Vanderbilt_Style}} | ||
<html> | <html> | ||
| + | |||
| + | <style> | ||
| + | .fit { | ||
| + | display:block; | ||
| + | width: 80%; | ||
| + | margin: 0 auto; | ||
| + | } | ||
| + | </style> | ||
| + | |||
<div id="breadcrumb_container" class="container" style="position:fixed; top:88px; z-index:2;"> | <div id="breadcrumb_container" class="container" style="position:fixed; top:88px; z-index:2;"> | ||
<div class="row"> | <div class="row"> | ||
| Line 14: | Line 24: | ||
<a href="https://2015.igem.org/Team:Vanderbilt/Project/Achievements" class="btn btn-default chosen">Achievements</a> | <a href="https://2015.igem.org/Team:Vanderbilt/Project/Achievements" class="btn btn-default chosen">Achievements</a> | ||
</div> | </div> | ||
| − | </div> | + | </div> <!-- /row --> |
| − | </ | + | </div> <!-- /breadcrumb_container --> |
| − | </ | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| + | <!-- adjust the text in the big space to match the page content --> | ||
<script> | <script> | ||
document.getElementById("header_text").innerHTML = "Achievements"; | document.getElementById("header_text").innerHTML = "Achievements"; | ||
</script> | </script> | ||
| − | <div class="row | + | <div class="row"> |
| − | <div class=" | + | <div class="col-md-10 col-md-offset-1" style="background-color: #44111a; height: 100%;"> |
| − | + | <h2>Sequence</h2> | |
| − | + | <div class='row'> | |
| − | + | <div class="col-md-9"> | |
| − | + | <ul> | |
| − | <h2>Sequence</h2> | + | <li>Invented an innovative software tool for minimizing any gene’s susceptibility to mutation</li> |
| − | < | + | <li>Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques</li> |
| − | <ul> | + | <li>Experimentally validated decreased mutation in optimized sequences with</li> |
| − | <li>Invented an innovative software tool for minimizing any gene’s susceptibility to mutation</li> | + | <ul> |
| − | <li>Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques</li> | + | <li>Multiple mutagen types (UV radiation, oxidation)</li> |
| − | <li>Experimentally validated decreased mutation in optimized sequences with</li> | + | <li>Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)</li> |
| − | <ul><li>Multiple mutagen types (UV radiation, oxidation)</li> | + | </ul> |
| − | <li>Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)</li></ul> | + | <li>Established high expression of optimized sequences</li> |
| − | <li>Established high expression of optimized sequences</li> | + | <li>Modeling and computational strategies for further improvements and expansion of software</li> |
| − | <li>Modeling and computational strategies for further improvements and expansion of software</li> | + | <li>Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection</li> |
| − | <li>Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection</li | + | </ul> |
| − | + | </div> | |
| − | + | <div class="col-md-3"> | |
| − | < | + | <img class="no-shadow fit" src="https://static.igem.org/mediawiki/2015/5/57/VU15_sequence_logo.png" /> |
| − | + | </div> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | </div> | + | |
| − | + | ||
| − | <div class="col- | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| − | < | + | <h2> Circuit </h2> |
| − | </ | + | <div class='row'> |
| − | + | <div class="col-md-9"> | |
| − | + | <ul> | |
| − | + | <li>Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits</li> | |
| − | + | <li>Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements </li> | |
| − | + | <li>Developed software for minimizing sequence homology between synthesized genes</li> | |
| − | <div class= | + | <li>Designed assay for quantifying the effect of lowering homology on rates of unwanted recombination</li> |
| − | + | <li>Constructed optimized circuit to demonstrate improved stability with VERT technique</li> | |
| − | + | <li>Validated bidirectional promoter for use with an antibiotic system to select against promoter mutation</li> | |
| − | + | </ul> | |
| − | + | </div> | |
| − | + | <div class="col-md-3"> | |
| − | < | + | <img class="no-shadow fit" src="https://static.igem.org/mediawiki/2015/1/14/VU15_circuit_logo.png" style="width:70%;"/> |
| − | < | + | </div> |
| − | + | ||
| − | + | ||
| − | < | + | |
</div> | </div> | ||
| − | < | + | <h2> Organism </h2> |
| − | + | <div class='row'> | |
| − | + | <div class="col-md-9"> | |
| − | < | + | <ul> |
| − | + | <li>Verified modeling predictions about population growth with simulated mutant cells</li> | |
| − | + | <li> Calculated the selective pressure against transgene expression by comparing experimental data with the Price equation</li> | |
| − | + | <li>Cloned five exogenous DNA repair enzymes </li> | |
| − | + | <li>Incorporated KIKO vector for genomic integration and simultaneous knock-out</li> | |
| − | + | <li>Designed “Incorruptible Cell” that commits suicide instead of passing on mutations</li> | |
| − | + | </ul> | |
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
</div> | </div> | ||
| − | <div class="col-md- | + | <div class="col-md-3"> |
| − | < | + | <img class="no-shadow fit" src="https://static.igem.org/mediawiki/2015/2/27/VU15_organism_logo.png" style="width:60%;"/> |
| − | + | <br> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
</div> | </div> | ||
| − | < | + | </div> |
| + | |||
| + | </html> | ||
| + | {{Template:Vanderbilt_Footer}} | ||
| + | <html> | ||
</html> | </html> | ||
Latest revision as of 03:57, 26 November 2015
Demons in the Code
Sequence
- Invented an innovative software tool for minimizing any gene’s susceptibility to mutation
- Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques
- Experimentally validated decreased mutation in optimized sequences with
- Multiple mutagen types (UV radiation, oxidation)
- Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)
- Established high expression of optimized sequences
- Modeling and computational strategies for further improvements and expansion of software
- Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection
Circuit
- Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits
- Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements
- Developed software for minimizing sequence homology between synthesized genes
- Designed assay for quantifying the effect of lowering homology on rates of unwanted recombination
- Constructed optimized circuit to demonstrate improved stability with VERT technique
- Validated bidirectional promoter for use with an antibiotic system to select against promoter mutation
Organism
- Verified modeling predictions about population growth with simulated mutant cells
- Calculated the selective pressure against transgene expression by comparing experimental data with the Price equation
- Cloned five exogenous DNA repair enzymes
- Incorporated KIKO vector for genomic integration and simultaneous knock-out
- Designed “Incorruptible Cell” that commits suicide instead of passing on mutations
