Difference between revisions of "Team:Vanderbilt/Project/Achievements"
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<li>Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits</li> | <li>Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits</li> | ||
<li>Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements </li> | <li>Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements </li> | ||
| − | <li>Developed | + | <li>Developed software for minimizing sequence homology between synthesized genes</li> |
| + | <li>Designed assay for quantifying the effect of lowering homology on rates of unwanted recombination</li> | ||
<li>Constructed optimized circuit to demonstrate improved stability with VERT technique</li> | <li>Constructed optimized circuit to demonstrate improved stability with VERT technique</li> | ||
| − | <li>Validated bidirectional promoter for use with system to select against promoter mutation</li> | + | <li>Validated bidirectional promoter for use with an antibiotic system to select against promoter mutation</li> |
</ul> | </ul> | ||
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| − | <img class="no-shadow fit" src="https://static.igem.org/mediawiki/2015/1/14/VU15_circuit_logo.png"/> | + | <img class="no-shadow fit" src="https://static.igem.org/mediawiki/2015/1/14/VU15_circuit_logo.png" style="width:70%;"/> |
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<h2> Organism </h2> | <h2> Organism </h2> | ||
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<div class="col-md-9"> | <div class="col-md-9"> | ||
<ul> | <ul> | ||
| + | <li>Verified modeling predictions about population growth with simulated mutant cells</li> | ||
| + | <li> Calculated the selective pressure against transgene expression by comparing experimental data with the Price equation</li> | ||
<li>Cloned five exogenous DNA repair enzymes </li> | <li>Cloned five exogenous DNA repair enzymes </li> | ||
<li>Incorporated KIKO vector for genomic integration and simultaneous knock-out</li> | <li>Incorporated KIKO vector for genomic integration and simultaneous knock-out</li> | ||
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Latest revision as of 03:57, 26 November 2015
Demons in the Code
Sequence
- Invented an innovative software tool for minimizing any gene’s susceptibility to mutation
- Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques
- Experimentally validated decreased mutation in optimized sequences with
- Multiple mutagen types (UV radiation, oxidation)
- Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)
- Established high expression of optimized sequences
- Modeling and computational strategies for further improvements and expansion of software
- Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection
Circuit
- Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits
- Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements
- Developed software for minimizing sequence homology between synthesized genes
- Designed assay for quantifying the effect of lowering homology on rates of unwanted recombination
- Constructed optimized circuit to demonstrate improved stability with VERT technique
- Validated bidirectional promoter for use with an antibiotic system to select against promoter mutation
Organism
- Verified modeling predictions about population growth with simulated mutant cells
- Calculated the selective pressure against transgene expression by comparing experimental data with the Price equation
- Cloned five exogenous DNA repair enzymes
- Incorporated KIKO vector for genomic integration and simultaneous knock-out
- Designed “Incorruptible Cell” that commits suicide instead of passing on mutations
