Difference between revisions of "Team:HSNU-TAIPEI/projectafla"

 
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<a>Project</a>
 
<a>Project</a>
 
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectoverview">Overview</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Description">Overview</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectBP">Benzo[A]Pyrene</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectBP">Benzo[A]Pyrene</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectcopper">Copper</a></li>
 
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectcadmium">Cadmium</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectcadmium">Cadmium</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectmercury">Mercury</a></li>
 
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectlead">Lead</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectlead">Lead</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectafla">Aflatoxin</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectafla">Aflatoxin</a></li>
 
</ul>
 
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</li>
 
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Design">Product</a></li>
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              <a>Parts</a>
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                <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Parts">Parts</a></li>
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                <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Basic_Part">Basic Part</a></li>
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                <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Composite_Part">Composite Part</a></li>
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                <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Part_Collection">Part Collection</a></li>
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              </ul>
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            </li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/labnotes">Notebook</a></li>
 
             <li class="list-item has-sub-nav">
 
             <li class="list-item has-sub-nav">
 
<a>Human Practice</a>
 
<a>Human Practice</a>
 
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/overview">Overview</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Practices">Overview</a></li>
 
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<a>Organization</a>
 
<a>Organization</a>
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                         <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/education/elementary">Elementary</a></li>
 
                         <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/education/elementary">Elementary</a></li>
 
                         <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/education/kindergarten">Kindergarten</a></li>
 
                         <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/education/kindergarten">Kindergarten</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/education/teacher">Teacher</a></li>
 
                       </ul>
 
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/ngo">NGO</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/ngo">NGO</a></li>
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<li class="list-item has-sub-nav">
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/industry/vendors">Industry</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/cooperation/meetup">Meet Up</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/cooperation/meetup">Meet Up</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/cooperation/ansianconference">Ansian Conference</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/cooperation/asianconference">Asian Conference</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/community">Community</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/community">Community</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/hp/lawyer">Lawyer</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
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            <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/labnotes">Notebook</a></li>
 
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            <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/parts">Parts</a></li>
 
 
             <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/achievement">Achievement</a></li>
 
             <li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/achievement">Achievement</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/product">Product</a></li>
 
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Team/members">Team</a></li>
 
<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Team/members">Team</a></li>
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<li class="list-item"><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Attributions">Attributions</a></li>
 
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       <li>
 
       <li>
 
         <span>Why do we detect Aflatoxin?</span>
 
         <span>Why do we detect Aflatoxin?</span>
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         <p class="article-p">黃麴毒素常見在花生、五穀雜糧裡，若放置在25～26度的地方時，就容易受到毒素的汙染，然而餿水油中含有很毒的黃麴毒素[1]，而黃麴毒素會累積在人體的人體的肝與 腎，有傷肝、肝硬化的風險，加上台灣B肝帶原者人數又多，若再受到餿水油的毒害，容易引發肝病或是造成洗腎。</p>
+
         <p class="article-p">Gutter oil contains aflatoxin which is very toxic and will accumulate in people’s liver and kidney. </p>
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         <p class="article-p">而黃麴毒素種類眾多，像黃麴毒素B1、B2、G1 及、G2 四種。黃麴毒素B1又是對於人體傷害最大[2]，所以我們決定偵測黃麴毒素B1。</p>
+
         <p class="article-p">In Taiwan, there are many people who are the asymptomatic carriers of hepatitis B, therefore if they be poisoned oil again, it will be easier for them to get liver disease or resulting in kidney dialysis.</p>
−
      </li>
+
         <p class="article-p">In our experiment, we try to detect Afb1 because it is the most toxic of aflatoxin. Our experiment was to find out more convenient detection method, so that everyone can easily detect their own oil if it is safe to eat.</p>
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      <li>
+
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        <span>Harm?</span>
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         <p class="article-p">黃麴毒素對身體的影響主要是在肝臟，若誤食含高劑量黃麴毒素的食物會造成肝臟壞死及急性的肝衰竭，爾後隨著病程的發展，有可能會進一步的發展成肝硬化或是引發肝癌[2]。</p>
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      </li>
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       <li>
 
       <li>
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         <span>Taiwan Regulations</span>
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         <span>Taiwanese regulations</span>
 +
<p class="article-p">&#9660;Table1: The regulation of Aflatoxin in Taiwan..</p>
 
         <div class="article-img"><img src="https://static.igem.org/mediawiki/2015/9/90/2015hsnu-aflatoxin_1.png"></div>
 
         <div class="article-img"><img src="https://static.igem.org/mediawiki/2015/9/90/2015hsnu-aflatoxin_1.png"></div>
 
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       </li>
 
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       <li>
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         <span>National Regulations</span>
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         <span>National regulations</span>
 
         <ol class="article-ol">
 
         <ol class="article-ol">
 
           <li>groundnuts, nuts, dried fruit and processed products of such products or food additives: B1 aflatoxin not exceed 2ug / kg (ppb), and B1, B2, G1, G2 sum not exceeding 4ug / kg.</li>
 
           <li>groundnuts, nuts, dried fruit and processed products of such products or food additives: B1 aflatoxin not exceed 2ug / kg (ppb), and B1, B2, G1, G2 sum not exceeding 4ug / kg.</li>
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     <h3 class="article-title">Circuit Design</h3>
 
     <h3 class="article-title">Circuit Design</h3>
 
     <div class="article-img"><img src="https://static.igem.org/mediawiki/2015/3/33/2015hsnu-aflatoxin_2.png"></div>
 
     <div class="article-img"><img src="https://static.igem.org/mediawiki/2015/3/33/2015hsnu-aflatoxin_2.png"></div>
 +
<p class="article-p">&#9650;Fig.1-1: Circuit design of detecting Lead ion.</p>
 
     <p class="article-p">When aflatoxin goes into the cell, CYP1A2 will oxidize the Aflatoxin B1, and then it will turn into Aflatoxin oxidation AFBO[5].</p>
 
     <p class="article-p">When aflatoxin goes into the cell, CYP1A2 will oxidize the Aflatoxin B1, and then it will turn into Aflatoxin oxidation AFBO[5].</p>
 
     <div class="article-img"><img src="https://static.igem.org/mediawiki/2015/b/bf/2015hsnu-aflatoxin_3.png"></div>
 
     <div class="article-img"><img src="https://static.igem.org/mediawiki/2015/b/bf/2015hsnu-aflatoxin_3.png"></div>
 
     <div class="article-img"><img src="https://static.igem.org/mediawiki/2015/e/ed/2015hsnu-aflatoxin_4.png"></div>
 
     <div class="article-img"><img src="https://static.igem.org/mediawiki/2015/e/ed/2015hsnu-aflatoxin_4.png"></div>
 +
<p class="article-p">&#9650;Fig.1-2: Circuit design of detecting Lead ion.</p>
 
     <p class="article-p">Single-strand DNA(ssDNA) can activate protein RecA. Ecoli has a sos response system, and there is a repressor LexA which inhibits the next reaction, so the fluorescent gene won’t express.But when the activated protein RecA comes and hydrolysis the represser LexA, the next reaction can work.[7]</p>
 
     <p class="article-p">Single-strand DNA(ssDNA) can activate protein RecA. Ecoli has a sos response system, and there is a repressor LexA which inhibits the next reaction, so the fluorescent gene won’t express.But when the activated protein RecA comes and hydrolysis the represser LexA, the next reaction can work.[7]</p>
 
     <p class="article-p">The Relationship between Cytochrome CYP3A4 in Vivo and the Toxicity of Aflatoxin B1 in Feedstuffs<br>ZHENG Lili1, ZHU Yujing1, SHAO Caimei2, ZHANG Yong1</p>
 
     <p class="article-p">The Relationship between Cytochrome CYP3A4 in Vivo and the Toxicity of Aflatoxin B1 in Feedstuffs<br>ZHENG Lili1, ZHU Yujing1, SHAO Caimei2, ZHANG Yong1</p>
 
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        <ul>
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                <li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectBP">Benzo[A]Pyrene</a></li>
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                <li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectcopper">Copper</a></li>
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                <li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectcadmium">Cadmium</a></li>
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                <li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectmercury">Mercury</a></li>
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                <li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectlead">Lead</a></li>
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                <li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectafla">Aflatoxin</a></li>
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        </ul>
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<div class="side-nav">
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<h4>Project</h4>
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<ul>
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<li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/Description">Overview</a></li>
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<li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectBP">Benzo[A]Pyrene</a></li>
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<li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectcopper">Copper</a></li>
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<li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectcadmium">Cadmium</a></li>
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<li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectmercury">Mercury</a></li>
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<li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectlead">Lead</a></li>
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<li><a href="https://2015.igem.org/Team:HSNU-TAIPEI/projectafla">Aflatoxin</a></li>
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</ul>
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<p><a href="http://www.hs.ntnu.edu.tw">HSNU | Taipei</a></p>
 
<p><a href="http://www.hs.ntnu.edu.tw">HSNU | Taipei</a></p>
 
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Latest revision as of 07:43, 26 November 2015

ProjectAflatoxin

Introduction

  1. Why do we detect Aflatoxin?

    Gutter oil contains aflatoxin which is very toxic and will accumulate in people’s liver and kidney.

    In Taiwan, there are many people who are the asymptomatic carriers of hepatitis B, therefore if they be poisoned oil again, it will be easier for them to get liver disease or resulting in kidney dialysis.

    In our experiment, we try to detect Afb1 because it is the most toxic of aflatoxin. Our experiment was to find out more convenient detection method, so that everyone can easily detect their own oil if it is safe to eat.

  2. Taiwanese regulations

    ▼Table1: The regulation of Aflatoxin in Taiwan..

  3. National regulations
    1. groundnuts, nuts, dried fruit and processed products of such products or food additives: B1 aflatoxin not exceed 2ug / kg (ppb), and B1, B2, G1, G2 sum not exceeding 4ug / kg.
    2. for the storage or not for direct human consumption's peanuts: B1 aflatoxin 8ug / kg, and B1, B2, G1, G2 sum shall not exceed 15ug / kg.
    3. for the storage or not for direct human consumption's nuts and dried fruits: B1 aflatoxin 5ug / kg, and B1, B2, G1, G2 sum shall not exceed 10ug / kg.
    4. Grains class for the direct human consumption: B1 aflatoxin 2ug / kg, or B1, B2, G1, G2 sum not exceeding 4ug / kg.
    5. Dairy: M1 aflatoxin not exceed 0.05 ug / kg.[4]

Circuit Design

▲Fig.1-1: Circuit design of detecting Lead ion.

When aflatoxin goes into the cell, CYP1A2 will oxidize the Aflatoxin B1, and then it will turn into Aflatoxin oxidation AFBO[5].

▲Fig.1-2: Circuit design of detecting Lead ion.

Single-strand DNA(ssDNA) can activate protein RecA. Ecoli has a sos response system, and there is a repressor LexA which inhibits the next reaction, so the fluorescent gene won’t express.But when the activated protein RecA comes and hydrolysis the represser LexA, the next reaction can work.[7]

The Relationship between Cytochrome CYP3A4 in Vivo and the Toxicity of Aflatoxin B1 in Feedstuffs
ZHENG Lili1, ZHU Yujing1, SHAO Caimei2, ZHANG Yong1

Reference

  • [1]Veronica S. Mary a, Ana Valdehita b, Jose M. Navas b, Hector R. Rubinstein a, Maria L. Fernandez-Cruz. Effects of aflatoxin B1, fumonisin B1 and their mixture on the aryl hydrocarbon receptor and cytochrome P450 1A induction
  • [2]Yan-Ping Du Hui-Lian Zhu. The possible mechanism of Aflatoxin B1 Hepatocarcinogenesis and preventive effects of phytochemicals
  • [3]Food hygiene standards - Food in aflatoxin limits (human) 82 1.4.4 Health Department Announcement fresh word No. 8189322
  • [4]EU animal and plant inspection and quarantine of food hygiene regulations cum Profile
  • [5]Veronica S. Mary a, Ana Valdehita b, Jose M. Navas b, Hector R. Rubinstein a,*Maria L. Fernandez-Cruz b. Effects of aflatoxin B1, fumonisin B1 and their mixture on the aryl hydrocarbon receptor and cytochrome P450 1A induction
  • [6]ZHENG Lili, ZHU Yujing, SHAO Caimei, ZHANG Yong The Relationship between Cytochrome CYP3A4 in Vivo and the Toxicity of Aflatoxin B1 in Feedstuffs
  • [7]Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1

Project