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| − | <h2> Welcome to iGEM 2015! </h2> | + | <h1> Methane Incorporated </h1> |
| − | <p>Your team has been approved and you are ready to start the iGEM season! </p> | + | <p>Methane (CH<sub>4</sub>) is the second most prevalent emitted greenhouse gas on earth. |
| | + | Today we are facing the challenge is that methane concentration in the atmosphere is increasing, |
| | + | leading to global warming. The global warming potential of methane is about 84 |
| | + | times higher than that of carbon dioxide (CO<sub>2</sub>) when compered over 20 years. |
| | + | Technical methods to reuse emitted methane gas are cost- and time- intensive and therefore rarely used.</p> |
| | + | <p>The goal of the project is to develop an <i>Escherichia coli</i> |
| | + | based model that filters methane out of the air and converts it into biomass. First part of the project is to |
| | + | express the soluble methane monooxygenase enzyme complex (sMMO) of the |
| | + | methanotroph <i>Methylococcus capsulatus</i> in <i>E. coli</i>. In order for the multisubunit |
| | + | enzyme complex to break down methane to methanol oxygen is required. Second |
| | + | part of the project is to establishing the Ribulose-Monophosphate (RuMP)- pathway |
| | + | in <i>E.coli.</i> The pathway is found in the methanotroph <i>Bacillus methanolicus</i> and enables |
| | + | <i>E. coli</i> to convert methanol into biomass.</p> |
| | + | <p>To test the functionality of the modified <i>E. coli</i>, the bacteria are grown in a closed |
| | + | system under methane rich conditions. Additionally, a filter was created that can |
| | + | contain the modified <i>E.coli</i> and evenly distribute methane. The filter can then be |
| | + | placed in areas where methane emission is a problem. </p> |
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| − | <h4>Before you start: </h4>
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| − | <p> Please read the following pages:</p>
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| − | <ul>
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| − | <li> <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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| − | <li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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| − | </ul>
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| − | <div class="highlightBox">
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| − | <h4> Styling your wiki </h4>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | </div>
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| − | <h4> Editing your wiki </h4>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2015.igem.org/wiki/index.php?title=Team:UiOslo_Norway&action=edit"> Click here to edit this page! </a></p>
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| − | <p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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| − | <h4>Templates </h4>
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| − | <p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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| − | <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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| − | <h4>Tips</h4>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <ul>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | <h4>Inspiration</h4>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| − | <h4> Uploading pictures and files </h4>
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| − | <p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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| − | <a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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Methane Incorporated
Methane (CH4) is the second most prevalent emitted greenhouse gas on earth.
Today we are facing the challenge is that methane concentration in the atmosphere is increasing,
leading to global warming. The global warming potential of methane is about 84
times higher than that of carbon dioxide (CO2) when compered over 20 years.
Technical methods to reuse emitted methane gas are cost- and time- intensive and therefore rarely used.
The goal of the project is to develop an Escherichia coli
based model that filters methane out of the air and converts it into biomass. First part of the project is to
express the soluble methane monooxygenase enzyme complex (sMMO) of the
methanotroph Methylococcus capsulatus in E. coli. In order for the multisubunit
enzyme complex to break down methane to methanol oxygen is required. Second
part of the project is to establishing the Ribulose-Monophosphate (RuMP)- pathway
in E.coli. The pathway is found in the methanotroph Bacillus methanolicus and enables
E. coli to convert methanol into biomass.
To test the functionality of the modified E. coli, the bacteria are grown in a closed
system under methane rich conditions. Additionally, a filter was created that can
contain the modified E.coli and evenly distribute methane. The filter can then be
placed in areas where methane emission is a problem.