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| − | <h2> Welcome to iGEM 2015! </h2> | + | <h2> Project Description </h2> |
| − | <p>Your team has been approved and you are ready to start the iGEM season! </p>
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| − | <h4>Before you start: </h4> | + | <p>Have you ever heard of the god of beginnings and transitions in in ancient Roman myth? He is Janus, who is usually depicted as having two faces, since he looks to the future and to the past. Our project is focused on another Janus, a kind of protein, who looks to the hydrophilicity and to the hydrophobicity. |
| − | <p> Please read the following pages:</p>
| + | Its scientific name is hydrophobin.</p> |
| − | <ul>
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| − | <li> <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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| − | <li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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| − | </ul>
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| − | <div class="highlightBox">
| + | <p>Hydrophobins are proteins that are produced by filamentous fungi. They are characterized by having a hydrophobic patch on one part of the surface. Just because of this, a sea of possibilities are opened. Our project is mainly focused on its modification and new applications.</p> |
| − | <h4> Styling your wiki </h4>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p> | + | |
| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | </div>
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| − | <h4> Editing your wiki </h4> | + | <li>1. Protein Modification |
| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
| + | Natural hydrophobins couldn't be expressed by E.coli because of error of fold. However, it really restricts its broader application. To improve this condition, we re-designed the structures of two kinds of hydrophobins, making expression in E.coli possible.</li> |
| − | <p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Tianjin&action=edit"> Click here to edit this page! </a></p>
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| − | <p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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| | + | <li>2.Protein Chip |
| | + | Another amazing characteristic of hydrophobins is that they are able to assemble spontaneously into amphipathic monolayers at hydrophobic-hydrophilic interfaces. Thus, we take this advantage to make a high-flux tumor detection chip. In this process, they could act as a medium in antibody-fixing by electrostatic force, which not only ensures the activity of antibody, but also improves the detections' accuracy rate.</li> |
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| − | <h4>Templates </h4> | + | <li>3.Stimulate Plastic Degradation |
| − | <p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
| + | Enzymatic hydrolysis of plastic has a broad developing prospect, however, the rate of hydrolysis is low due to many reasons. Some research has published that the cutinase degrading plastic could be enhanced by hydrophobins. In our experiment, we make them into a fusion to test if the effect could be better. </li> |
| − | <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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| − | <h4>Tips</h4>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <ul>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | <h4>Inspiration</h4>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| − | <h4> Uploading pictures and files </h4>
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| − | <p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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| − | <a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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| − | </div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
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| | + | <li>4.Protein Extraction |
| | + | Because of hydrophobins' amphipathicity they could be used as a special purification tag for protein, and the purification system could be as simple as oil and water. With help of this, we could even achieve the recycle of enzymes used for plastic degradation.</li> |
| | </html> | | </html> |
Add a banner to your wiki!
You can make the image 980px by 200px
Remember to call the file: "Team_Tianjin_banner.jpg"
Project Description
Have you ever heard of the god of beginnings and transitions in in ancient Roman myth? He is Janus, who is usually depicted as having two faces, since he looks to the future and to the past. Our project is focused on another Janus, a kind of protein, who looks to the hydrophilicity and to the hydrophobicity.
Its scientific name is hydrophobin.
Hydrophobins are proteins that are produced by filamentous fungi. They are characterized by having a hydrophobic patch on one part of the surface. Just because of this, a sea of possibilities are opened. Our project is mainly focused on its modification and new applications.
1. Protein Modification
Natural hydrophobins couldn't be expressed by E.coli because of error of fold. However, it really restricts its broader application. To improve this condition, we re-designed the structures of two kinds of hydrophobins, making expression in E.coli possible.
2.Protein Chip
Another amazing characteristic of hydrophobins is that they are able to assemble spontaneously into amphipathic monolayers at hydrophobic-hydrophilic interfaces. Thus, we take this advantage to make a high-flux tumor detection chip. In this process, they could act as a medium in antibody-fixing by electrostatic force, which not only ensures the activity of antibody, but also improves the detections' accuracy rate.
3.Stimulate Plastic Degradation
Enzymatic hydrolysis of plastic has a broad developing prospect, however, the rate of hydrolysis is low due to many reasons. Some research has published that the cutinase degrading plastic could be enhanced by hydrophobins. In our experiment, we make them into a fusion to test if the effect could be better.
4.Protein Extraction
Because of hydrophobins' amphipathicity they could be used as a special purification tag for protein, and the purification system could be as simple as oil and water. With help of this, we could even achieve the recycle of enzymes used for plastic degradation.