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| + | In November 1973, Stanley Cohen and Herb Boyer marked the start of biotechnology by describing a way to construct new functional bacterial plasmids in vitro, which we now call traditional cloning. In the forty years that followed, research efforts have led to a variation in available cloning methods. Currently available methods include methods independent of ligation, independent of restriction enzymes and even independent of the use of a chassis. | ||
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| + | iGEM teams in particular have experimented and pioneered many of these cloning methods: Cambridge teams have pioneered Gibson Assembly, Freiburg introduced iGEM to the Golden Gate Standard and Lethbridge familiarized iGEM with Ligation Independent Cloning. Novel cloning methods have thus started to play a major role within the iGEM competition, and we think they will become even more important in the future. But as a new iGEM team, still unfamiliar with DNA Recombinant technology, let alone newer cloning methods, we couldn’t see forest for the trees. To enable future iGEM teams to make a more informed choice on assembly standards and methods, we are compiling a cloning guide. | ||
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| + | To be able to compile such a guide we rely heavily on collaborations with other teams. They are the ones who can guide you through the cloning methods, having hands-on experience with many of these methods. Therefore, we have reached out to many other iGEM teams. An overview of the participating teams is presented below: | ||
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Revision as of 12:19, 13 July 2015
Cloning Guide
In November 1973, Stanley Cohen and Herb Boyer marked the start of biotechnology by describing a way to construct new functional bacterial plasmids in vitro, which we now call traditional cloning. In the forty years that followed, research efforts have led to a variation in available cloning methods. Currently available methods include methods independent of ligation, independent of restriction enzymes and even independent of the use of a chassis.
iGEM teams in particular have experimented and pioneered many of these cloning methods: Cambridge teams have pioneered Gibson Assembly, Freiburg introduced iGEM to the Golden Gate Standard and Lethbridge familiarized iGEM with Ligation Independent Cloning. Novel cloning methods have thus started to play a major role within the iGEM competition, and we think they will become even more important in the future. But as a new iGEM team, still unfamiliar with DNA Recombinant technology, let alone newer cloning methods, we couldn’t see forest for the trees. To enable future iGEM teams to make a more informed choice on assembly standards and methods, we are compiling a cloning guide.
To be able to compile such a guide we rely heavily on collaborations with other teams. They are the ones who can guide you through the cloning methods, having hands-on experience with many of these methods. Therefore, we have reached out to many other iGEM teams. An overview of the participating teams is presented below: