Difference between revisions of "Team:UC San Diego/Description"

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<h2> Project Description </h2>
 
  
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
 
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<li> A clear and concise description of your project.</li>
 
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
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<h4>Advice on writing your Project Description</h4>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<h4>References</h4>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
 
  
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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---------------->
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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            <h2>Abstract</h2>
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            <p>The lux system’s characteristic bioluminescent reaction has been the subject of intense research due to the potential of luciferase as a reporter. Compared to common fluorescent reporter systems, luciferase assays offer increased sensitivity and do not run the risk of inducing phototoxicity in the system being analyzed. As a result, they offer a powerful tool to researchers seeking to monitor a wide variety of biological processes. However, the environmental conditions, such as the amount of metabolites available, affects the bioluminescent reaction. Therefore, our objective is to modify the expression of the genes lux C, D, and E to determine the rate-limiting steps.</p>
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<p>We will develop and assemble three plasmids that expressed the aforementioned genes in a 1:1:2 ratio.. The plasmid vectors will be inserted into Saccharomyces cerevisiae, and the variation in light yield will be measured. To further our findings, we will create an experimentally validated mathematical model that provides an in depth in silico analysis. With these results, we can optimize the pathway by controlling the expression of certain enzymes.</p>
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Revision as of 20:01, 13 July 2015

Project

post header

Abstract

The lux system’s characteristic bioluminescent reaction has been the subject of intense research due to the potential of luciferase as a reporter. Compared to common fluorescent reporter systems, luciferase assays offer increased sensitivity and do not run the risk of inducing phototoxicity in the system being analyzed. As a result, they offer a powerful tool to researchers seeking to monitor a wide variety of biological processes. However, the environmental conditions, such as the amount of metabolites available, affects the bioluminescent reaction. Therefore, our objective is to modify the expression of the genes lux C, D, and E to determine the rate-limiting steps.

We will develop and assemble three plasmids that expressed the aforementioned genes in a 1:1:2 ratio.. The plasmid vectors will be inserted into Saccharomyces cerevisiae, and the variation in light yield will be measured. To further our findings, we will create an experimentally validated mathematical model that provides an in depth in silico analysis. With these results, we can optimize the pathway by controlling the expression of certain enzymes.

Contact Us: ucsd.igem.2015@gmail.com