Difference between revisions of "Team:CHINA CD UESTC"

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<head>
 
<head>
  
−
<link rel="stylesheet" href="https://2015.igem.org/Team:CHINA_CD_UESTC/Template:RightStyle.css?action=raw&ctype=text/css" type="text/css" />
 
 
</head>
 
</head>
 +
 +
<style type="text/css">
 +
/* General Slides */
 +
 +
#content{
 +
height:90px;
 +
  padding: 0px 0px 0px 0px;
 +
  border-left: 0px solid #444444;
 +
  border-right: 0px solid #444444;
 +
}
 +
.slide{
 +
background-attachment: fixed;
 +
width:100%;
 +
height:100%;
 +
position: relative;
 +
padding:0 10px 0 10px;
 +
margin: 0 10px 0 10px;
 +
z-index:12;
 +
overflow:hidden;
 +
}
 +
 +
 +
h2 {
 +
display:block;
 +
margin:0 0 23px;
 +
padding:0;
 +
text-transform:none;
 +
text-align:left;
 +
font-style:normal;
 +
border-bottom:1px solid #e0e0e0;
 +
font-family: Helvetica ;
 +
font-size: 70px;
 +
line-height: 82px;
 +
color:#343434;
 +
font-weight: 300;
 +
}
 +
 +
 +
h3 {
 +
margin:0;
 +
padding:40px 0 32px 0;
 +
text-transform:uppercase;
 +
text-align:center;
 +
font-family: Helvetica ;
 +
font-weight:800;
 +
font-size:32px;
 +
font-style:normal;
 +
line-height:36px;
 +
color:#3e3e3e;
 +
}
 +
 +
h4 {
 +
margin:0;
 +
padding:0 0 15px;
 +
text-transform:none;
 +
text-align:left;
 +
font-family: Helvetica ;
 +
font-weight:300;
 +
font-size:32px;
 +
font-style:normal;
 +
line-height:36px;
 +
color:#222;
 +
}
 +
 +
img.icon_img {
 +
float:left;
 +
margin-right:20px;
 +
margin-top:20px;
 +
margin-bottom:25px;
 +
}
 +
 +
.effect_2 {
 +
background:url(../images/effect_2.png) repeat left top;
 +
position:relative;
 +
top:0;
 +
bottom:-57px;
 +
height:100%;
 +
z-index:999 !important;
 +
}
 +
 +
 +
/* ____________________________________ Slide 2 标准文本____________________________________ */
 +
 +
#slide2 {
 +
background:url(../images/slide2_bg.jpg) repeat left top;
 +
width:100%;
 +
padding:20px 0 20px;
 +
}
 +
 +
.det_inf {margin-bottom:24px;
 +
}
 +
.det_inf li {margin-bottom:10px;
 +
}
 +
.det_inf li a  {
 +
color:#929292;
 +
}
 +
.det_inf li a:hover {
 +
color:#f14833;
 +
}
 +
li.phone {
 +
background:url(../images/inf_icon.png) no-repeat 0 0;
 +
padding-left:44px;
 +
}
 +
li.address {
 +
background:url(../images/inf_icon.png) no-repeat 0 -99px;
 +
padding-left:44px;
 +
}
 +
li.pers_mail {
 +
background:url(../images/inf_icon.png) no-repeat 0 -188px;
 +
padding-left:44px;
 +
}
 +
li.pers_site {
 +
background:url(../images/inf_icon.png) no-repeat 0 -265px;
 +
padding-left:44px;
 +
}
 +
 +
 +
/* ___________________________________ //Slide 2 ___________________________________ */
 +
 +
/*____________________________grid.css________________________________*/
 +
 +
/* Computer */
 +
.grid_1 { width: 6.5%; }
 +
.grid_2 { width: 15%; }
 +
.grid_3 { width: 23.5%; }
 +
.grid_4 { width: 32%; }
 +
.grid_5 { width: 40.5%; }
 +
.grid_6 { width: 49%; }
 +
.grid_7 { width: 57.5%; }
 +
.grid_8 { width: 66%; font-family: Verdana,Arial  ;
 +
}
 +
.grid_9 { width: 74.5%; }
 +
.grid_10 { width: 83%; }
 +
.grid_11 { width: 91.5%; }
 +
.grid_12 { width: 100%; }
 +
 +
.grid_1,
 +
.grid_2,
 +
.grid_3,
 +
.grid_4,
 +
.grid_5,
 +
.grid_6,
 +
.grid_7,
 +
.grid_8,
 +
.grid_9,
 +
.grid_10,
 +
.grid_11,
 +
.grid_12 {
 +
margin: 0 20px 20px 0;
 +
float: left;
 +
display: block;
 +
}
 +
 +
.alpha {margin-left:0;}
 +
.omega {margin-right:0;}
 +
.gallery-list {margin:0;padding:0;}
 +
.little_respond {display:none;}
 +
 +
.container{
 +
width: 100%;
 +
max-width: 1000px;
 +
margin: auto;
 +
 +
}
 +
 +
 +
.clear{clear:both;display:block;overflow:hidden;visibility:hidden;width:0;height:0}
 +
.clearfix:after{position:relative;z-index:99;clear:both;content:' ';display:block;font-size:0;line-height:0;visibility:hidden;width:0;height:0}* html .clearfix,*:first-child+html .clearfix{zoom:1}
 +
 +
@media (max-width: 1024px) {
 +
.slide{background-attachment: scroll;background-position:0 0 !important;
 +
}
 +
}
 +
 +
@media (min-width: 767px) and (max-width: 1023px) {
 +
#firstTitle {font-size: 80px;}
 +
 +
 +
.gallery-list .omega {margin-right:10px;}
 +
 +
.filtr_bg {left:50%;}
 +
 +
h4 {font-size:27px;}
 +
 +
#slide1 h1 {font-size:50px;line-height:65px;}
 +
#slide1 p {font-size:30px;line-height:36px;}
 +
 +
.padding_slide1 {padding:150px 0 30px;}
 +
 +
.pers_inf li span {width:130px;}
 +
 +
.exhib_cont {width:282px;}
 +
.exhib_block p {width:250px;}
 +
.exhib_date_top {left:49.3%;}
 +
 +
 +
/* ______________ //Gallery _______________ */
 +
/* ___________ SERV_BLOCK __________ */
 +
.servc_block p {font-size:28px;}
 +
 +
/* __________ //SERV_BLOCK _________ */
 +
 +
.container { width: 100%; }
 +
.container .alpha        { margin-left: 0; margin-right: 5px; }
 +
.container .omega        { margin-right: 0; }
 +
.gallery-list .omega {margin-right:10px;}
 +
.container .respond_pad {padding-right:20px;}
 +
 +
.grid_3 { width: 166px; }
 +
.grid_4 { width: 228px; }
 +
.grid_5      { width: 290px; }
 +
.grid_6      { width: 352px; }
 +
.grid_7  { width: 414px; }
 +
.grid_8  { width: 475px; }
 +
.grid_9 { width: 538px; }
 +
.grid_12 { width: 724px; }
 +
}
 +
 +
@media (max-width: 766px) {
 +
        #firstTitle {font-size: 80px;}
 +
 +
h2 {padding-bottom:10px;font-size:45px;line-height:58px;}
 +
 +
 +
#slide12 .foto {float:none;}
 +
#slide12 h5 {font-size:26px;}
 +
 +
 +
.inset_shadow {top:76px;bottom:0;}
 +
 +
.menu {position:fixed;top:0;margin-top:0 !important;}
 +
#undefined-sticky-wrapper {height:0 !important;}
 +
 +
 +
 +
}
 +
 +
@media (min-width: 480px) and (max-width: 766px) {
 +
h1 {font-size:46px;line-height:115px;padding-bottom:0;}
 +
#firstTitle {font-size: 60px;}
 +
 +
 +
.filtr_bg {width:420px;left:50%;}
 +
.slogan_text {font-size:21px;}
 +
.left_bord, .right_bord {margin-bottom:6px;}
 +
#slide1 h1 {font-size:30px;line-height:40px;}
 +
#slide1 p {font-size:21px;line-height:42px;}
 +
 +
#slide1 .button {margin:50px auto 0;}
 +
#slide1 .button:hover {margin:51px auto 0;}
 +
 +
.exhib_line_center {left:4px;height:79%;}
 +
.exhib_date_top {left:0;}
 +
.exhib_p_top {margin-left:120px;}
 +
.exhib_date_left, .exhib_date_right {
 +
top:-30px;
 +
left:-42px;
 +
height:115px;
 +
padding-left:40px;
 +
text-align:left;
 +
}
 +
#slide8 .exhib_date_left, #slide8 .exhib_date_right {
 +
top:-30px;
 +
left:-42px;
 +
height:115px;
 +
padding-left:40px;
 +
text-align:left;
 +
}
 +
 +
#slide8 .exhib_block .fleft {margin-left:42px;}
 +
#slide8 .exhib_date_top {width:155px;}
 +
#slide8 .exhib_p_top {margin-left:170px;}
 +
#slide8 .exhib_line_center {height:83%;}
 +
 +
.exhib_cont, .exhib_block .fleft {
 +
margin-left:42px;
 +
margin-top:50px;
 +
width:230px;
 +
}
 +
 +
.gallery-list {margin-right:0 !important;}
 +
 +
/* _____________ menu ______________ */
 +
#nav {margin:0;}
 +
 +
/* ____________ //menu _____________ */
 +
 +
/* _______________ JCAROUSEL ________________ */
 +
 +
/* ______________ //JCAROUSEL _______________ */
 +
 +
.container { width: 100%; }
 +
 +
.grid_1,
 +
.grid_2,
 +
.grid_3,
 +
.grid_4,
 +
.grid_5,
 +
.grid_6,
 +
.grid_7,
 +
.grid_8,
 +
.grid_9,
 +
.grid_10,
 +
.grid_11,
 +
.grid_12 {width:420px;}
 +
 +
.fright{float:none;}
 +
.fleft{float:none;}
 +
 +
}
 +
 +
@media (max-width : 479px) {
 +
  #firstTitle {font-size: 60px;}
 +
 +
h2 {font-size:40px;line-height:50px;margin:0;}
 +
h4 {font-size:22px;line-height:28px;}
 +
 +
 +
 +
.padding_slide1 {padding:188px 0 100px;}
 +
 +
.filtr_bg {width:295px;}
 +
.slogan_text {font-size:21px;}
 +
.left_bord, .right_bord {margin-bottom:6px;width:70px;}
 +
#slide1 h1 {font-size:24px;line-height:30px;}
 +
#slide1 p {font-size:21px;line-height:42px;}
 +
#slide1 .button {margin:40px auto 0;}
 +
#slide1 .button:hover {margin:41px auto 0;}
 +
 +
 +
 +
.educ_block p {font-size:16px;}
 +
 +
.pers_inf li {font-size:12px;}
 +
.pers_inf li span {width:110px;}
 +
 +
 +
.exhib_line_center {left:4px;height:79%;}
 +
.exhib_date_top {left:0;}
 +
.exhib_p_top {margin-left:42px;padding-top:30px;}
 +
.exhib_date_left, .exhib_date_right {
 +
top:-30px;
 +
left:-42px;
 +
height:115px;
 +
padding-left:40px;
 +
text-align:left;
 +
}
 +
#slide8 .exhib_date_left, #slide8 .exhib_date_right {
 +
top:-30px;
 +
left:-42px;
 +
height:115px;
 +
padding-left:40px;
 +
text-align:left;
 +
}
 +
 +
#slide8 .exhib_block .fleft {margin-left:42px;}
 +
#slide8 .exhib_date_top {width:155px;}
 +
#slide8 .exhib_p_top {margin-left:42px;padding-top:30px;}
 +
#slide8 .exhib_line_center {height:84%;}
 +
 +
.exhib_cont, .exhib_block .fleft {
 +
margin-left:42px;
 +
margin-top:50px;
 +
width:210px;
 +
}
 +
 +
.gallery-list {margin-right:0 !important;}
 +
 +
.client_foto img {width:300px;}
 +
 +
/* _______________ JCAROUSEL ________________ */
 +
 +
/* ______________ //JCAROUSEL _______________ */
 +
 +
/* ___________ SERV_BLOCK __________ */
 +
 +
 +
/* __________ //SERV_BLOCK _________ */
 +
 +
.container { width: 100%; }
 +
 +
.grid_1,
 +
.grid_2,
 +
.grid_3,
 +
.grid_4,
 +
.grid_5,
 +
.grid_6,
 +
.grid_7,
 +
.grid_8,
 +
.grid_9,
 +
.grid_10,
 +
.grid_11,
 +
.grid_12 {
 +
width:300px;
 +
}
 +
 +
.fright{float:none;}
 +
.fleft{float:none;}
 +
 +
}
 +
 +
@media (max-width : 399px) {
 +
.btn_dropdown {margin-right:3px;}
 +
  #firstTitle {font-size: 60px;}
 +
 +
 +
.gallery-list {width:270px;margin-left:15px !important;}
 +
 +
 +
#map_block iframe {height:300px !important;}
 +
 +
 +
.contact_form input[type="text"], .contact_form textarea {width:88%;}
 +
 +
 +
.container { width: 100%; }
 +
 +
.grid_1,
 +
.grid_2,
 +
.grid_3,
 +
.grid_4,
 +
.grid_5,
 +
.grid_6,
 +
.grid_7,
 +
.grid_8,
 +
.grid_9,
 +
.grid_10,
 +
.grid_11,
 +
.grid_12 {
 +
width:295px;
 +
}
 +
 +
}
 +
 +
 +
 +
 +
 +
/*____________________________//grid.css________________________________*/
 +
 +
</style>
 
<style type="text/css">
 
<style type="text/css">
 
/****************
 
/****************
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</div>
 
</div>
−
<div class="grid_4 omega">
 
−
<h4>Personal Info</h4>
 
−
<ul class="det_inf">
 
−
<li class="phone">(+1) 245 5462 252</li>
 
−
<li class="address">3301 Lorem Ipsum, Dolor Sit St</li>
 
−
<li class="pers_mail"><a href="javascript:void(0);">support@lorem.com</a></li>
 
−
<li class="pers_site"><a href="javascript:void(0);">www.lorem.com</a></li>
 
−
</ul>
 
−
<h4>My Recent Tweets</h4>
 
−
<div class="ftr_twit">
 
−
<div class="tweet_block"><p class="loading">loading tweets...</p></div>
 
−
</div>
 
−
</div>
 
−
<div class="clear"></div>
 
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</div>
 
−
 
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</div>
 
−
</div>
 
  
−
 
−
<div class="slide" id="slide2" data-slide="4" data-stellar-background-ratio="0.5" style="background-position: 0px 669px;">
 
−
<div class="container clearfix">
 
−
 
−
<div id="content" class="grid_12">
 
−
<h2>About Me</h2>
 
−
</div>
 
−
<div class="clear"></div>
 
−
 
−
<div id="content">
 
−
<div class="grid_8">
 
−
<h4>Biography</h4>
 
−
<p>Morbi rutrum, elit ac fermentum egestas, tortor ante vestibulum est, eget scelerisque nisl velit eget tellus. Fusce porta facilisis luctus. Integer neque dolor, rhoncus nec euismod eget, pharetra et tortor. Nulla id pulvinar nunc. Vestibulum auctor nisl vel lectus ullamcorper sed pellentesque dolor eleifend. Praesent lobortis magna vel diam mattis sagittis.</p>
 
−
<p>Mauris porta odio eu risus scelerisque id facilisis ipsum dictum vitae volutpat. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed pulvinar neque eu purus sollicitudin et sollicitudin dui ultricies. Maecenas cursus auctor tellus sit amet blandit. Maecenas a erat ac nibh molestie interdum. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Sed lorem enim, ultricies sed sodales id, convallis molestie ipsum. Morbi eget dolor ligula. Vivamus accumsan rutrum nisi nec elementum. Pellentesque at nunc risus. Phasellus ullamcorper bibendum varius.  Quisque quis ligula sit amet felis ornare porta. Aenean viverra lacus et mi elementum mollis. Praesent eu justo elit.</p>
 
−
</div>
 
−
<div class="grid_4 omega">
 
−
<h4>Personal Info</h4>
 
−
<ul class="det_inf">
 
−
<li class="phone">(+1) 245 5462 252</li>
 
−
<li class="address">3301 Lorem Ipsum, Dolor Sit St</li>
 
−
<li class="pers_mail"><a href="javascript:void(0);">support@lorem.com</a></li>
 
−
<li class="pers_site"><a href="javascript:void(0);">www.lorem.com</a></li>
 
−
</ul>
 
−
<h4>My Recent Tweets</h4>
 
−
<div class="ftr_twit">
 
−
<div class="tweet_block"><p class="loading">loading tweets...</p></div>
 
−
</div>
 
−
</div>
 
 
<div class="clear"></div>
 
<div class="clear"></div>
 
 
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</div>
 
</div>
 
</div>
 
</div>
−
 
−
 
−
<div class="slide" id="slide2" data-slide="6" data-stellar-background-ratio="0.5" style="background-position: 0px 669px;">
 
−
<div class="container clearfix">
 
−
 
−
<div id="content" class="grid_12">
 
−
<h2>About Me</h2>
 
−
</div>
 
−
<div class="clear"></div>
 
−
 
−
<div id="content">
 
−
<div class="grid_8">
 
−
<h4>Biography</h4>
 
−
<p>Morbi rutrum, elit ac fermentum egestas, tortor ante vestibulum est, eget scelerisque nisl velit eget tellus. Fusce porta facilisis luctus. Integer neque dolor, rhoncus nec euismod eget, pharetra et tortor. Nulla id pulvinar nunc. Vestibulum auctor nisl vel lectus ullamcorper sed pellentesque dolor eleifend. Praesent lobortis magna vel diam mattis sagittis.</p>
 
−
<p>Mauris porta odio eu risus scelerisque id facilisis ipsum dictum vitae volutpat. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed pulvinar neque eu purus sollicitudin et sollicitudin dui ultricies. Maecenas cursus auctor tellus sit amet blandit. Maecenas a erat ac nibh molestie interdum. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Sed lorem enim, ultricies sed sodales id, convallis molestie ipsum. Morbi eget dolor ligula. Vivamus accumsan rutrum nisi nec elementum. Pellentesque at nunc risus. Phasellus ullamcorper bibendum varius.  Quisque quis ligula sit amet felis ornare porta. Aenean viverra lacus et mi elementum mollis. Praesent eu justo elit.</p>
 
−
</div>
 
−
<div class="grid_4 omega">
 
−
<h4>Personal Info</h4>
 
−
<ul class="det_inf">
 
−
<li class="phone">(+1) 245 5462 252</li>
 
−
<li class="address">3301 Lorem Ipsum, Dolor Sit St</li>
 
−
<li class="pers_mail"><a href="javascript:void(0);">support@lorem.com</a></li>
 
−
<li class="pers_site"><a href="javascript:void(0);">www.lorem.com</a></li>
 
−
</ul>
 
−
<h4>My Recent Tweets</h4>
 
−
<div class="ftr_twit">
 
−
<div class="tweet_block"><p class="loading">loading tweets...</p></div>
 
−
</div>
 
−
</div>
 
−
<div class="clear"></div>
 
−
 
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</div>
 
−
 
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</div>
 
−
</div>
 
−
 
−
 
−
<div class="slide" id="slide2" data-slide="4" data-stellar-background-ratio="0.5" style="background-position: 0px 669px;">
 
−
<div class="container clearfix">
 
−
 
−
<div id="content" class="grid_12">
 
−
<h2>About Me</h2>
 
−
</div>
 
−
<div class="clear"></div>
 
−
 
−
<div id="content">
 
−
<div class="grid_8">
 
−
<h4>Biography</h4>
 
−
<p>Morbi rutrum, elit ac fermentum egestas, tortor ante vestibulum est, eget scelerisque nisl velit eget tellus. Fusce porta facilisis luctus. Integer neque dolor, rhoncus nec euismod eget, pharetra et tortor. Nulla id pulvinar nunc. Vestibulum auctor nisl vel lectus ullamcorper sed pellentesque dolor eleifend. Praesent lobortis magna vel diam mattis sagittis.</p>
 
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Revision as of 05:18, 14 July 2015

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PROJECT intro

  We are a skillful and persistent group of nine Finns. We started as a group of students who didn't really know each other, assuming that we were going to spend our summer studying synthetic biology with strange colleagues. In the end we got a bunch of new friends and (in addition to studying synthetic biology) we just might have spent one of the best summers of our lives.

The new Magical Bacteria: Magnetotactic E.Coli

2015-UESTC TEAM

There is a special microbe in nature which can be attracted by magnet ---Magnetotactic bacterium(MTB). Scientists are very interested in it because its Magnetotaxis. Magnetosome, some Fe3O4 nanocrystals covered by membrane with unprecedented magnetic properties, is the reason why MTB has the ability to be attracted by magnet. The process of magnetosome formation which is controled by many related gene cluster is very complex , including----- membrane invaginate forming vesicle---absorbing Iron ions -----Iron ions being oxidized into Fe3O4 ------magnetosome connect to each other and form nanopaticle chain.

A lot of great value about magnetotaxis application can be excavated . But MTB is anaerobic, and hard to be raised , also hard to transform. Obviously it is hard to modify and research MTB at molecular level . the large size and the complexity of related gene cluster is also a reason restricted the use of magnetotaxis.

In our project, we are aiming to transfer mamAB、mamGFDC、mms6、mamXYZ four main gene clusters which are related to the formation of magnetosome into E. Coli through recombinant plasmid. The E.Coli will produce magnetosome and abtain the magnetotactic ability. The Magnetotactic E.Coli will be applied in a variety of areas. We will explore the potential in the applaction of Microbial Fuel Cell.

In addition to this ,we will research several genes related to the producing of magnetosome, find out the Specific function in this processing.