Difference between revisions of "Team:IIT Kharagpur"

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<h2> Welcome to iGEM 2015! </h2>
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<img src="https://static.igem.org/mediawiki/2015/b/b5/Team_IIT_Kharagpur_bg1.jpg" class="img-responsive">
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    <h1>IDEA SUMMARY
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<h4>Before you start: </h4>
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</h1>
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<p> Please read the following pages:</p>
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<p>Problems like food spoilage are a major concern in our lives mainly because such problems have a direct impact on the health of a person, which is the most important thing. Food spoilage is caused by the biochemical activities of a microbial community that grows in the prevailing conditions.
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<h4> Styling your wiki </h4>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h4> Editing your wiki </h4>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:IIT_Kharagpur&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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<h4>Templates </h4>
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Some of the present tools that help in identifying food spoilage are CFU analysis, ATP bioluminescence assays, volatile compound analysis, Electrical impedance measurement etc.
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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</p>
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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<h3>Flaws</h3>
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<p> These are time-consuming, tedious, expensive and not accurate enough.
  
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What if we are given a tool which could monitor food spoilage and in itself is harmless to the body?
  
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<h4>Tips</h4>
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Let us help you know about a cool application of Biotechnology as marker of food spoilage!
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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</p>
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<ul>
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<h2>Project Overview:
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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</h2>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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It is known that bacteria often communicate with each other through Quorum sensing, where the communication is through certain signal molecules that freely diffuse into the environment. As the number of bacteria in a region increases, the concentration of signal molecules also increases.  
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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After a certain threshold concentration, these signal molecules bind to promoter and induce the gene of interest. This helps them to express their genes together and cause a larger impact.  They also “become aware” of the concentration of their own species and of other species in their vicinity.
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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Recent research has shown the possibility of the involvement of quorum sensing in food spoilage. The enzymatic activities that account for quality degradation of the food product are regulated by quorum sensing activity. Also, the quorum molecules (autoinducers) are detected in spoiled food products.
  
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<h4>Inspiration</h4>
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We aim to make a genetically engineered bacteria that senses the concentration of other food spoiling bacteria and expresses a colour pigment.
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
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The engineered E.coli  senses the concentration of other bacteria and triggers the expression of lycopene (red-coloured) pigment which can be easily observed with the naked eye.
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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<h3>Application</h3>
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<p>Packets will have a pouch with a sample of product and our engineered bacteria. Detection of AHLs (in the case of food contamination) in the product sample will trigger the expression of the lycopene gene that will produce the red-coloured pigment (easily observable to the human eye).
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<p>Copyright &copy; 2015 iGEM Team IIT Kharagpur</p>
  
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Revision as of 09:48, 14 July 2015

IDEA SUMMARY

Problems like food spoilage are a major concern in our lives mainly because such problems have a direct impact on the health of a person, which is the most important thing. Food spoilage is caused by the biochemical activities of a microbial community that grows in the prevailing conditions. Some of the present tools that help in identifying food spoilage are CFU analysis, ATP bioluminescence assays, volatile compound analysis, Electrical impedance measurement etc.

Flaws

These are time-consuming, tedious, expensive and not accurate enough. What if we are given a tool which could monitor food spoilage and in itself is harmless to the body? Let us help you know about a cool application of Biotechnology as marker of food spoilage!

Project Overview:

It is known that bacteria often communicate with each other through Quorum sensing, where the communication is through certain signal molecules that freely diffuse into the environment. As the number of bacteria in a region increases, the concentration of signal molecules also increases. After a certain threshold concentration, these signal molecules bind to promoter and induce the gene of interest. This helps them to express their genes together and cause a larger impact. They also “become aware” of the concentration of their own species and of other species in their vicinity. Recent research has shown the possibility of the involvement of quorum sensing in food spoilage. The enzymatic activities that account for quality degradation of the food product are regulated by quorum sensing activity. Also, the quorum molecules (autoinducers) are detected in spoiled food products. We aim to make a genetically engineered bacteria that senses the concentration of other food spoiling bacteria and expresses a colour pigment. The engineered E.coli senses the concentration of other bacteria and triggers the expression of lycopene (red-coloured) pigment which can be easily observed with the naked eye.

Application

Packets will have a pouch with a sample of product and our engineered bacteria. Detection of AHLs (in the case of food contamination) in the product sample will trigger the expression of the lycopene gene that will produce the red-coloured pigment (easily observable to the human eye).

Copyright © 2015 iGEM Team IIT Kharagpur