Difference between revisions of "Template:Team:TU Eindhoven/Timeline HTML"
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<li><span class="activiteit">Making a glycerol stock & sending the DNA for sequencing</span></li> | <li><span class="activiteit">Making a glycerol stock & sending the DNA for sequencing</span></li> | ||
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| + | <h4>Week 29</h4> | ||
| + | <h3> - Hopeful results </h3> | ||
| + | <br \> | ||
| + | <span class="activiteit"> Traditional cloning: <br />The sequencing results are positive, everything is built in correctly.</span> | ||
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Revision as of 14:16, 14 July 2015
Timeline
Week 26
- Preparing for take-off
- Inventory of the lab supplies
- Pouring LB Agar plates
- Amplification of the pET-Duet-1 vector
- Assessment of the safety requirements
- Preparing stocks for antibiotics, glycerol, LB & MilliQ
Week 27
- The Clone Wars
Gibson Assembly:
- Linearizing pET-Duet-1 for Gibson Assembly
- Our first Gibson Assembly!
Traditional cloning:
- Amplification of the pET-Duet-1 vector
- Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning
Week 28
- Et tu, pET-Duet?
Gibson Assembly:
- Linearizing pET-Duet-1 for Gibson Assembly and debugging
Traditional cloning:
- Amplification of the inserts
- Xbal & Pstl digestion of the pET-Duet-1 vector (MCS-1)
- Xba1 & Pst1 digestion of the inserts (MCS-1)
- Nde1 & Kpn1 digestion of the inserts (MCS-2)
- Ligation
- Transformation in NB
- Colony PCR & gel electrophorese: The inserts are succesfully ligated
- Culturing of the colonies with the correct plasmid
- Making a glycerol stock & sending the DNA for sequencing
Week 29
- Hopeful results
Traditional cloning:
The sequencing results are positive, everything is built in correctly.